FastQCFastQC Report
Fri 27 May 2016
SRR522053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44193611
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN924480.2091886087335113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG53400.011.15113520
GATCGGA57100.010.11415521
CGGTTCA67300.09.9160021
AATGCCG67600.09.76899313
CGAGATC60250.09.66156118
GAGATCG63200.09.25398919
CCGAGAT74650.08.31611917
ATGCCGA79300.08.16070514
TGCCGAG83700.07.95154315
GCCGAGA86450.07.683537516
ATCGGAA74300.07.676286722
GAGCGGT72800.07.42439429
AGCGGTT69100.07.364322730
AAGAGCG82850.07.196229527
TCGGAAG86650.06.947792523
TCAACGC158750.06.75412759
GCGGTTC60500.06.55981711
CAACGCA164000.06.47893210
AGTGGTA158750.06.38776542
GGTATCA165500.06.38509045
Produced by FastQC (version 0.11.5)