##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522053_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44193611
Sequences flagged as poor quality	0
Sequence length	36
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.27912233738945	33.0	10.0	38.0	2.0	39.0
2	25.253817933094446	33.0	2.0	38.0	2.0	39.0
3	25.033778887178965	33.0	2.0	38.0	2.0	39.0
4	24.780700653766445	33.0	2.0	38.0	2.0	39.0
5	24.558940838756083	33.0	2.0	38.0	2.0	39.0
6	23.92532721528458	32.0	2.0	38.0	2.0	39.0
7	23.66750963165241	32.0	2.0	38.0	2.0	39.0
8	23.445735742209433	32.0	2.0	38.0	2.0	39.0
9	23.220068348793674	31.0	2.0	38.0	2.0	39.0
10	23.036135653182992	31.0	2.0	38.0	2.0	39.0
11	22.887849580791215	31.0	2.0	38.0	2.0	39.0
12	22.68194060901699	31.0	2.0	38.0	2.0	39.0
13	22.544341511265056	31.0	2.0	38.0	2.0	39.0
14	22.42855128991383	31.0	2.0	38.0	2.0	39.0
15	22.287454514635613	31.0	2.0	38.0	2.0	39.0
16	22.108969823715015	31.0	2.0	38.0	2.0	39.0
17	21.957888120977486	30.0	2.0	38.0	2.0	39.0
18	21.75698129759073	30.0	2.0	38.0	2.0	39.0
19	21.642651830374305	29.0	2.0	38.0	2.0	39.0
20	21.493896391494236	29.0	2.0	38.0	2.0	39.0
21	21.307924871764836	29.0	2.0	37.0	2.0	39.0
22	21.09920139813875	29.0	2.0	37.0	2.0	39.0
23	20.947017364116274	28.0	2.0	37.0	2.0	39.0
24	20.76077770608064	27.0	2.0	36.0	2.0	39.0
25	20.60847679996097	27.0	2.0	36.0	2.0	39.0
26	20.331696633705718	27.0	2.0	36.0	2.0	39.0
27	20.09537057290928	27.0	2.0	36.0	2.0	39.0
28	20.00151053508617	26.0	2.0	36.0	2.0	39.0
29	19.813681416528738	26.0	2.0	36.0	2.0	39.0
30	19.61454885413188	25.0	2.0	35.0	2.0	38.0
31	19.511763159611466	25.0	2.0	36.0	2.0	39.0
32	19.232033290965973	23.0	2.0	35.0	2.0	38.0
33	19.004099212440458	23.0	2.0	35.0	2.0	38.0
34	18.758494570629225	23.0	2.0	35.0	2.0	38.0
35	18.47791788274554	21.0	2.0	35.0	2.0	38.0
36	18.457923024212707	20.0	2.0	35.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-24.08246655285063
1	2	-23.069091055537204
1	3	-22.60854353642265
1	4	-22.33524399043125
1	5	-22.127847965389076
1	6	-21.3399742711983
1	7	-21.092880009757785
1	8	-20.86928247650064
1	9	-20.672149593721308
1	10	-20.456987568519896
1	11	-19.75854938379262
1	12	-19.554874861902306
1	13	-19.43237222918613
1	14	-19.316001860881318
1	15	-19.206147437121174
1	16	-19.139533970171875
1	17	-19.008420170586692
1	18	-18.81824254073262
1	19	-18.734272001526172
1	20	-18.605616281401755
1	21	-18.517530145590595
1	22	-18.33877820367388
1	23	-18.176091394808438
1	24	-18.00080804477951
1	25	-17.866230943336564
1	26	-17.621821088246868
1	27	-17.398926901464353
1	28	-17.297483644102876
1	29	-17.125050552491494
1	30	-16.940578868705835
1	31	-16.83884882438579
1	32	-16.56993465280002
1	33	-16.337377775030255
1	34	-16.108154227371102
1	35	-15.84327823950982
1	36	-15.829760710131062
2	1	-1.3395283543131242
2	2	-1.3112344192780618
2	3	-1.2313646958484554
2	4	-1.1794316665265043
2	5	-1.206741880719548
2	6	-1.1659209692552572
2	7	-1.063528084563842
2	8	-1.064425847123296
2	9	-1.1946472719393952
2	10	-1.038026135997935
2	11	-1.1381200171830876
2	12	-0.9431658845824735
2	13	-0.9419790353813298
2	14	-0.9389012969755761
2	15	-1.192407141216961
2	16	-0.9732732341078396
2	17	-0.9016684186221973
2	18	-0.8157717031690304
2	19	-1.1333964856334013
2	20	-0.8316118777844963
2	21	-0.9623632650648268
2	22	-0.8786522171489146
2	23	-1.1210958402570625
2	24	-0.7698196948363822
2	25	-0.8935560788371291
2	26	-1.3499250809184744
2	27	-0.8286951567897702
2	28	-1.3958035262087805
2	29	-1.0111474211738027
2	30	-1.0627529617203884
2	31	-1.0724855902983244
2	32	-1.20103215681592
2	33	-0.9945973779379891
2	34	-0.9473878098752948
2	35	-0.908845999289781
2	36	-1.0061333145745408
3	1	-1.0427469843596775
3	2	-1.0730654809735256
3	3	-0.9452963231159153
3	4	-0.9414066354649684
3	5	-0.9803961011478322
3	6	-0.8870644887708607
3	7	-0.9969722724301313
3	8	-0.9893870379635423
3	9	-1.0134053086619588
3	10	-1.0720596556114081
3	11	-0.8514144381461897
3	12	-0.8570495798781366
3	13	-0.9279674715658288
3	14	-0.9176613808463472
3	15	-0.8468909015700952
3	16	-0.8499186125618756
3	17	-0.8608783862114855
3	18	-0.7492125753560295
3	19	-0.8463553914876343
3	20	-0.6770779732156456
3	21	-0.8154145039298442
3	22	-0.6562124255093131
3	23	-0.7007237164868201
3	24	-0.7333854199594647
3	25	-0.648180564721418
3	26	-0.8951804413248716
3	27	-0.5925866541649754
3	28	-0.8289566294913122
3	29	-0.809603484167809
3	30	-0.9047602608985557
3	31	-0.8756070163041763
3	32	-0.9275960844791911
3	33	-0.9518025072448282
3	34	-1.147299892936374
3	35	-1.102585274607538
3	36	-1.2808593388989458
4	1	-0.9306937148179095
4	2	-0.9650828999849637
4	3	-0.819961800176177
4	4	-0.867947500967265
4	5	-0.7571649935104929
4	6	-0.7688760286755354
4	7	-0.8802266560865988
4	8	-0.9834964964470245
4	9	-0.715097692590124
4	10	-0.7919965656223305
4	11	-0.6166740111980076
4	12	-0.7076734829832425
4	13	-0.6931309895795046
4	14	-0.6222542203443098
4	15	-0.7996476054831128
4	16	-0.7747230002257943
4	17	-0.7761273636361459
4	18	-0.66747148354656
4	19	-0.6703350588877299
4	20	-0.733234916836107
4	21	-0.7205521565410073
4	22	-0.5939832765852735
4	23	-0.6376799779714091
4	24	-0.4350539926826187
4	25	-0.5257175025920553
4	26	-0.4831938217968421
4	27	-0.5054474179972743
4	28	-0.4892702490067151
4	29	-0.5542304225045811
4	30	-0.4939549006856616
4	31	-0.6890844268178249
4	32	-0.5416465802982344
4	33	-0.5655673385771038
4	34	-0.5654231411550796
4	35	-0.5821449193780204
4	36	-0.6218688810326576
5	1	-0.8057250416239441
5	2	-0.7183134388947074
5	3	-0.6411276325675708
5	4	-0.5736033182592486
5	5	-0.6463869814135812
5	6	-0.6397087173313842
5	7	-0.6656392625969012
5	8	-0.6232140289936083
5	9	-0.5277809283093546
5	10	-0.5200559498246058
5	11	-0.5097787337814275
5	12	-0.4832910203525209
5	13	-0.41944131113395144
5	14	-0.5610531758482864
5	15	-0.5396846914080271
5	16	-0.5048934201644357
5	17	-0.4590608917353798
5	18	-0.25997891851605104
5	19	-0.3712157653633845
5	20	-0.3344239787798635
5	21	-0.20594296705228388
5	22	-0.39941927299821245
5	23	-0.2986662485933884
5	24	-0.15719228383754924
5	25	-0.2091576659386547
5	26	-0.28390996208873887
5	27	-0.11170735843047908
5	28	-0.25916402761823676
5	29	-0.13115869305950412
5	30	-0.09549879107871106
5	31	-0.23465320703514791
5	32	-0.44478901247662606
5	33	-0.16163251293628278
5	34	-0.3617242052415435
5	35	-0.3791708211672109
5	36	-0.11386029499165318
6	1	-0.9254156722734308
6	2	-0.8260617697692361
6	3	-0.7756083377795484
6	4	-0.9185767336104291
6	5	-0.8900645099449314
6	6	-0.8067918521767865
6	7	-0.9833062076758665
6	8	-0.8453466392877402
6	9	-0.7820236680221626
6	10	-0.8073826335153846
6	11	-0.7937881651182153
6	12	-0.7409386448742588
6	13	-0.8108286223320675
6	14	-0.8681879651587998
6	15	-0.7371169623851586
6	16	-0.5350551725506527
6	17	-0.5982735290234125
6	18	-0.5423492934625251
6	19	-0.7466551323494244
6	20	-0.6204227689458612
6	21	-0.5620474493127929
6	22	-0.5818245404446394
6	23	-0.5103312379389529
6	24	-0.5789577093849054
6	25	-0.4489290676786766
6	26	-0.5670633013872006
6	27	-0.4577896863920685
6	28	-0.5587391364927292
6	29	-0.5979702278042183
6	30	-0.6033297387498955
6	31	-0.6212534785599573
6	32	-0.7676428448904922
6	33	-0.5388120675481574
6	34	-0.5656042862140325
6	35	-0.6292652866359774
6	36	-0.7093248038104463
7	1	-0.7356958113172034
7	2	-0.6049842948674318
7	3	-0.686180288741209
7	4	-0.5306687621246766
7	5	-0.5208838030474894
7	6	-0.628958302679294
7	7	-0.7502982676292014
7	8	-0.6259422356563107
7	9	-0.46870104782042077
7	10	-0.5656549223659404
7	11	-0.5666670001197041
7	12	-0.46934200492328415
7	13	-0.4877504282052776
7	14	-0.6332166681299043
7	15	-0.409169774248511
7	16	-0.42932208509366276
7	17	-0.5404561855063434
7	18	-0.3736680227440772
7	19	-0.46420045208187943
7	20	-0.3779031262342549
7	21	-0.20774957632895763
7	22	-0.3015991964867979
7	23	-0.2958316870626909
7	24	-0.33214869474221587
7	25	-0.18445649381079932
7	26	-0.2655202616722008
7	27	-0.36213011131842165
7	28	-0.26590784165792414
7	29	-0.30561198871342654
7	30	-0.4916524033437639
7	31	-0.5241278985397706
7	32	-0.3933757591314553
7	33	-0.5265044601453326
7	34	-0.37865864645267067
7	35	-0.3126706428167765
7	36	-0.45153105198818366
8	1	-0.7754174472914137
8	2	-0.7997199512955788
8	3	-0.7923651638872293
8	4	-0.7734555722337682
8	5	-0.8859133857200945
8	6	-0.6624478551569055
8	7	-0.6072669545792095
8	8	-0.6382202022831542
8	9	-0.8993671659155993
8	10	-0.8085665780632922
8	11	-0.5034934487700546
8	12	-0.569415680803985
8	13	-0.6304487984198168
8	14	-0.5952097752921901
8	15	-0.5382599211121359
8	16	-0.5264722118912175
8	17	-0.4624657771197853
8	18	-0.4444999757857744
8	19	-0.4551897978945547
8	20	-0.4303411079496051
8	21	-0.5977155803065592
8	22	-0.2726896138762065
8	23	-0.3880252853987898
8	24	-0.3981639107552546
8	25	-0.30940613324979793
8	26	-0.35136885455053957
8	27	-0.42770549676167846
8	28	-0.3486721822152177
8	29	-0.31810213644090624
8	30	-0.219827222180907
8	31	-0.3326337684076037
8	32	-0.2930530102985678
8	33	-0.25631821531020904
8	34	-0.24110862012518197
8	35	-0.15248744578298457
8	36	-0.2614901765133588
9	1	-0.5273003559536491
9	2	-0.5320808221759386
9	3	-0.5720880264806496
9	4	-0.5489339764319077
9	5	-0.5145008917786207
9	6	-0.6663203517214704
9	7	-0.4266146808428033
9	8	-0.7489572162887228
9	9	-0.5067411696691408
9	10	-0.5814255765377503
9	11	-0.4258821024113253
9	12	-0.3602433181297684
9	13	-0.42522941304061135
9	14	-0.3142413349969715
9	15	-0.40134357953430566
9	16	-0.3604597931336393
9	17	-0.3863004652161557
9	18	-0.3217098116788826
9	19	-0.39581240450502264
9	20	-0.334289429890795
9	21	-0.3824443470356975
9	22	-0.5336515697990052
9	23	-0.43215017065098493
9	24	-0.3945967119518059
9	25	-0.5486458038059894
9	26	-0.6221633688994359
9	27	-0.3071837711292744
9	28	-0.8150387828443684
9	29	-0.5731062496352308
9	30	-0.5237974617445751
9	31	-0.39881300278786114
9	32	-0.4752551152378288
9	33	-0.4454779959825501
9	34	-0.5130158891162644
9	35	-0.8204762357651099
9	36	-0.7564511803360681
10	1	-0.41976571900885773
10	2	-0.5356356965784883
10	3	-0.400580535148066
10	4	-0.4158217791044372
10	5	-0.4627738562640076
10	6	-0.4284772855655703
10	7	-0.46362971314740165
10	8	-0.30036966731800163
10	9	-0.33149323317216783
10	10	-0.29344791067282827
10	11	-0.17432525199059867
10	12	-0.338177364194884
10	13	-0.1777720706116135
10	14	-0.13601467810697798
10	15	0.0047114382168267355
10	16	-0.21869679939615239
10	17	-0.03325242391242966
10	18	-0.025436783006178132
10	19	-0.16643190308198896
10	20	-0.06090420615259262
10	21	-0.044017564758110694
10	22	-0.07815371866434973
10	23	-0.15499482498987405
10	24	-0.12783307138338174
10	25	-0.043666822635525904
10	26	-0.021001114789505948
10	27	-0.17074746698896348
10	28	0.0036714865333706825
10	29	0.03875665848972076
10	30	-0.00901143318755615
10	31	0.06914260893069724
10	32	0.2711512682848287
10	33	0.18185721089853857
10	34	0.21773242928983905
10	35	0.12070679928000416
10	36	0.24490489104921664
11	1	-0.2072653263270574
11	2	-0.21382083706269128
11	3	-0.09042484061498612
11	4	-0.13979924739309268
11	5	-0.11775246903984282
11	6	-0.12466339039052343
11	7	-0.18651572676104422
11	8	-0.1976034140527041
11	9	-0.2894108480240938
11	10	-0.2785390962584948
11	11	-0.18986881168559933
11	12	-0.1627170086087517
11	13	-0.12792601746708954
11	14	-0.032331110415690034
11	15	-0.13779164457889337
11	16	-0.2246156502505805
11	17	-0.038986780580376745
11	18	-0.2685944244614653
11	19	-0.08329464950979215
11	20	-0.05179676739906469
11	21	-0.27418288554932246
11	22	-0.1965381509103885
11	23	-0.2541134950274859
11	24	-0.40214835904901136
11	25	-0.022816473415709027
11	26	-0.10770297782786287
11	27	-0.27123744228060787
11	28	-0.11426531184753941
11	29	-0.12457743565241586
11	30	-0.261372345294415
11	31	-0.23911425181471202
11	32	-0.05350714337244611
11	33	-0.18813575123025217
11	34	-0.17710736744948719
11	35	-0.14645584301209524
11	36	0.08385899175021194
12	1	-0.3602158610396202
12	2	-0.3909369097956841
12	3	-0.2158971471645863
12	4	-0.24250053713028308
12	5	-0.29947957479935994
12	6	-0.1678123960891007
12	7	-0.18214018884786043
12	8	-0.17197552867515498
12	9	-0.1483163641130929
12	10	-0.19867189487598935
12	11	-0.10749882507379027
12	12	-0.1250838391882283
12	13	-0.1230573806018711
12	14	-0.1777971338394444
12	15	-0.05424474284425429
12	16	-0.05944493203952206
12	17	-0.0841301107633079
12	18	-0.07445989587942137
12	19	-0.1570688667579283
12	20	-0.09556165050677379
12	21	-0.0651208098555891
12	22	-0.12190011745244078
12	23	-0.1444350914743957
12	24	-0.03473066615787701
12	25	-0.21610325271876363
12	26	-0.12893776166197313
12	27	-0.26827891862387077
12	28	-0.24829133875651976
12	29	-0.2546104164652405
12	30	-0.34040719127232677
12	31	-0.22311226045809107
12	32	-0.2968490419381524
12	33	-0.18320659690169805
12	34	0.023342449652663078
12	35	-0.37073833768241116
12	36	-0.107025198034993
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117	32	-0.9376640377841525
117	33	-0.9094963298511907
117	34	-0.8536198258005463
117	35	-0.8137474428894969
117	36	-0.7126281705328523
118	1	-1.1108478068715115
118	2	-1.2493998594170463
118	3	-1.1918999153570873
118	4	-1.2594609885661505
118	5	-1.3220471838115877
118	6	-1.2566631264302934
118	7	-1.305353027846973
118	8	-1.3941214006365463
118	9	-1.267780013984055
118	10	-1.5205527309285962
118	11	-1.2970638144685047
118	12	-1.1881340457621405
118	13	-1.3495298086714627
118	14	-1.2927079059947602
118	15	-1.2711880911397913
118	16	-1.2061018218433546
118	17	-1.1832427671485277
118	18	-1.0119399954508879
118	19	-1.1409202495904793
118	20	-0.9974079527777064
118	21	-1.152119681009271
118	22	-1.2409519919940024
118	23	-1.0343049273986225
118	24	-1.0704297544385923
118	25	-1.0167461645341227
118	26	-1.1981815450986701
118	27	-1.12666370318372
118	28	-1.2148513518654873
118	29	-1.1175541322090972
118	30	-1.2495222026520096
118	31	-1.274242588061199
118	32	-1.3608275911243126
118	33	-1.188064449469227
118	34	-1.2665482051814152
118	35	-1.1570330230886796
118	36	-1.1545248533778434
119	1	-1.235585969352087
119	2	-1.337661924666115
119	3	-1.2958154825620163
119	4	-1.4523269256353153
119	5	-1.2669667395719593
119	6	-1.418638708187185
119	7	-1.30087454837928
119	8	-1.4167102648003471
119	9	-1.3447942040445042
119	10	-1.3791536141903435
119	11	-1.4004098649809542
119	12	-1.9708156249224942
119	13	-1.4457108559908711
119	14	-1.3818757632205312
119	15	-1.4890551062465107
119	16	-1.37846315095166
119	17	-1.303615155216093
119	18	-1.3289062302245966
119	19	-1.4198056994221844
119	20	-1.2900889765947383
119	21	-1.372430733006663
119	22	-1.3999805192871193
119	23	-1.3250642809173705
119	24	-1.3807385663489846
119	25	-1.2795116301990639
119	26	-1.3329949137391388
119	27	-1.2833581715247
119	28	-1.483213605390464
119	29	-1.300179965554289
119	30	-1.445770234920822
119	31	-1.5718277546297763
119	32	-1.601956568445619
119	33	-1.4740785415509308
119	34	-1.3489235664439079
119	35	-1.335779082825237
119	36	-1.3480078660344752
120	1	-24.08246655285063
120	2	-23.069091055537204
120	3	-22.84805867222352
120	4	-22.595631622504886
120	5	-22.37527131406348
120	6	-21.75065035857757
120	7	-21.492763331207193
120	8	-21.274286689892744
120	9	-21.052359615112376
120	10	-20.867858120150153
120	11	-20.720110283513886
120	12	-20.51549585107713
120	13	-20.380385452755196
120	14	-20.263658566656908
120	15	-20.125347540835442
120	16	-19.94688471885924
120	17	-19.797361636847146
120	18	-19.592728831002923
120	19	-19.48273249287251
120	20	-19.331648562467418
120	21	-19.14590896195152
120	22	-18.943950952750175
120	23	-18.791765213438115
120	24	-18.603809276386432
120	25	-18.456494766503738
120	26	-18.184795094858654
120	27	-17.94848287475793
120	28	-17.854753366018997
120	29	-17.667314116373
120	30	-17.47098011327704
120	31	-17.365522837479716
120	32	-17.089510720732864
120	33	-16.864537948751654
120	34	-16.61781261850353
120	35	-16.339939936534517
120	36	-16.31961615837116
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	1.2013423E7
3	593759.0
4	627674.0
5	728756.0
6	622592.0
7	299152.0
8	325965.0
9	382610.0
10	296949.0
11	227849.0
12	242576.0
13	261693.0
14	250649.0
15	230330.0
16	238397.0
17	250950.0
18	257657.0
19	265266.0
20	296885.0
21	344369.0
22	391418.0
23	435328.0
24	485099.0
25	537592.0
26	597886.0
27	663806.0
28	745093.0
29	854238.0
30	996490.0
31	1184876.0
32	1427686.0
33	1756955.0
34	2200410.0
35	2720330.0
36	3227192.0
37	3405182.0
38	2600556.0
39	1205718.0
40	255.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.541278405766104	29.967317190442945	23.538983863835654	26.952420539955295
2	25.428109093304414	29.861910381531853	24.14169557441978	20.568284950743955
3	24.32779255207376	27.77403195003099	24.67062319837517	23.227552299520074
4	26.944098669351373	26.61490039984691	23.43164688856076	23.009354042240954
5	26.980280873035195	27.86185021336695	24.246710526555415	20.911158387042438
6	25.8113653220368	26.674903383023345	22.230783195188998	25.28294809975085
7	24.61041400396984	26.215309549796316	25.430383523833367	23.743892922400477
8	24.553553901608304	25.854774009933372	25.148242612641987	24.443429475816338
9	23.81961333643385	25.560464489292578	25.484143207335197	25.13577896693837
10	24.054790367148236	26.93728372020685	23.798267703208662	25.209658209436252
11	24.39668715240092	26.209126835810437	24.360098397872807	25.03408761391584
12	24.19917073130099	25.84757283359675	24.93872809719948	25.01452833790278
13	24.02686209407737	26.05417601218652	25.02538422074067	24.89357767299544
14	23.975784791065042	26.110526923833106	25.16016444758156	24.753523837520294
15	23.602596069521645	25.8019408765432	25.349645772493368	25.24581728144179
16	23.746245166891434	25.743026007926535	25.193131157132925	25.317597668049107
17	23.792660048220444	25.598490333749773	25.220413059457975	25.388436558571808
18	23.548730991711704	25.437085203607264	25.064350085311503	25.94983371936953
19	23.896980076290607	25.50681074497751	25.078583591913358	25.517625586818525
20	23.803234414145255	25.555232758398827	25.127139909796504	25.514392917659418
21	23.721769381244982	25.500125082677943	25.003244927531288	25.774860608545787
22	23.734074966632797	25.45626634543917	24.873249280098957	25.936409407829075
23	23.782174906862153	25.47688546989378	24.982808649373588	25.758130973870475
24	23.797419082456546	25.349813381890556	24.749039238703872	26.10372829694902
25	23.98533902837464	25.32089872997029	24.614370123591574	26.0793921180635
26	24.065542485316392	25.324211490595903	24.737138412243514	25.873107611844194
27	24.025208773026836	25.140668842429843	24.594152169604016	26.239970214939305
28	23.989266173645746	25.29309756715876	24.682460402666575	26.035175856528912
29	23.924289034933256	25.341557794662727	24.77366699100645	25.960486179397563
30	23.79763871418748	25.20755080039879	24.720644397673464	26.27416608774027
31	23.888210175675944	25.20875565021835	24.687323530862766	26.21571064324294
32	23.852036849779335	25.192723593747203	24.728472445563447	26.226767110910014
33	23.814811066784248	25.054229555884216	24.620589216943994	26.51037016038754
34	23.882025903592137	25.023135837642496	24.4854302662553	26.609407992510064
35	23.774684295987804	25.086630014190025	24.600454974933434	26.538230714888737
36	23.8203178846508	24.964153430855706	24.53121996659179	26.6843087179017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1015022.0
1	1194375.0
2	1373728.0
3	1373728.0
4	1091556.5
5	809385.0
6	809385.0
7	633290.5
8	457196.0
9	457196.0
10	364086.0
11	270976.0
12	270976.0
13	228478.0
14	185980.0
15	179345.0
16	172710.0
17	172710.0
18	198892.5
19	225075.0
20	225075.0
21	285550.5
22	346026.0
23	346026.0
24	429327.0
25	512628.0
26	638911.0
27	765194.0
28	765194.0
29	946497.5
30	1127801.0
31	1127801.0
32	1331244.5
33	1534688.0
34	1534688.0
35	1756946.0
36	1979204.0
37	1979204.0
38	2195575.0
39	2411946.0
40	2641977.5
41	2872009.0
42	2872009.0
43	3083365.0
44	3294721.0
45	3294721.0
46	3449299.0
47	3603877.0
48	3603877.0
49	3667201.5
50	3730526.0
51	3701523.0
52	3672520.0
53	3672520.0
54	3533107.0
55	3393694.0
56	3393694.0
57	3183758.0
58	2973822.0
59	2973822.0
60	2688494.0
61	2403166.0
62	2403166.0
63	2105072.5
64	1806979.0
65	1535333.5
66	1263688.0
67	1263688.0
68	1044310.5
69	824933.0
70	824933.0
71	666829.0
72	508725.0
73	508725.0
74	404652.0
75	300579.0
76	235617.0
77	170655.0
78	170655.0
79	132315.0
80	93975.0
81	93975.0
82	71591.5
83	49208.0
84	49208.0
85	36638.5
86	24069.0
87	24069.0
88	17325.5
89	10582.0
90	7429.5
91	4277.0
92	4277.0
93	3008.0
94	1739.0
95	1739.0
96	1353.0
97	967.0
98	967.0
99	1154.0
100	1341.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.8777284345467944
2	3.1979215276162885
3	4.431572699501745
4	5.752913017223237
5	6.8127585229457726
6	7.575859777559249
7	8.05828471450319
8	8.47572061943524
9	8.79397250430611
10	8.981151144223087
11	9.145960034811367
12	9.268563729721023
13	9.32371876106707
14	9.354653096801707
15	9.42466321659029
16	9.517547683532808
17	9.586679848360886
18	9.69500546130978
19	9.676161108446196
20	9.704909607861643
21	9.766088134323308
22	9.791840725574563
23	9.802312827526134
24	9.884148186035308
25	9.924242216821794
26	9.920567477502573
27	9.929989653934367
28	10.00124203473665
29	9.977012288043174
30	9.969499889927528
31	10.010290854033176
32	9.991914894666563
33	10.000622035615057
34	9.976584624415507
35	10.004409913460115
36	9.966569602108324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	4.4193611E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.04297125204955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.47367908358156	98.5216873781077
2	0.4170689905576326	0.8261550408384185
3	0.07808979186388285	0.23202735031959246
4	0.01617493251465925	0.06408053504212952
5	0.005465262492353836	0.02706479179575528
6	0.002195350917578837	0.013046044669075283
7	0.0023186125647389273	0.01607495943158549
8	0.0013963784764057033	0.011064117863650465
9	4.518683743662652E-4	0.004027894777086153
>10	0.0029795957817189325	0.0541366241470472
>50	9.322930195729098E-5	0.006490351263433167
>100	8.129432557956756E-5	0.012134989899378806
>500	3.3123593686549685E-6	0.0016993814331014623
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.296888210660083E-6	0.2103105404120906
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	92448	0.2091886087335113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8102164134087165E-5	3.3941557751413434E-5	0.0	0.0	4.525541033521791E-6
2	2.489047568436985E-5	7.240865653634866E-5	2.2627705167608956E-6	4.525541033521791E-6	4.525541033521791E-6
3	2.7153246201130747E-5	1.1313852583804478E-4	2.2627705167608956E-6	1.1313852583804478E-5	9.051082067043582E-6
4	3.167878723465254E-5	1.4708008358945823E-4	2.2627705167608956E-6	1.1313852583804478E-5	1.583939361732627E-5
5	3.3941557751413434E-5	1.7875887082411076E-4	2.2627705167608956E-6	1.3576623100565373E-5	1.583939361732627E-5
6	4.072986930169612E-5	2.081748875420024E-4	2.2627705167608956E-6	1.583939361732627E-5	1.583939361732627E-5
7	4.525541033521791E-5	2.3985367477665493E-4	2.2627705167608956E-6	1.8102164134087165E-5	1.8102164134087165E-5
8	5.20437218855006E-5	2.4664198632693763E-4	2.2627705167608956E-6	1.8102164134087165E-5	2.489047568436985E-5
9	9.95619027374794E-5	2.60218609427503E-4	2.2627705167608956E-6	1.8102164134087165E-5	2.9416016717891642E-5
10	6.132108100422027E-4	2.7832077356159014E-4	2.2627705167608956E-6	2.2627705167608956E-5	3.3941557751413434E-5
11	7.376631884640519E-4	3.190506428632863E-4	2.2627705167608956E-6	2.489047568436985E-5	4.2992639818457016E-5
12	8.078090744836397E-4	3.416783480308952E-4	4.525541033521791E-6	3.167878723465254E-5	4.751818085197881E-5
13	8.44013402751814E-4	3.5751774164822153E-4	4.525541033521791E-6	3.8467098784935225E-5	5.656926291902239E-5
14	8.802177310199883E-4	3.8014544681583046E-4	4.525541033521791E-6	4.525541033521791E-5	6.788311550282687E-5
15	0.0025818211596241817	3.959848404331567E-4	4.525541033521791E-6	4.97809513687397E-5	7.693419756987045E-5
16	0.0026519670456437694	4.2540085715104837E-4	4.525541033521791E-6	5.4306492402261494E-5	8.145973860339224E-5
17	0.0027922588176829454	4.593424149024618E-4	6.788311550282687E-6	6.335757446930508E-5	9.051082067043582E-5
18	0.0029778060000573386	5.068605957544406E-4	6.788311550282687E-6	7.240865653634866E-5	9.503636170395761E-5
19	0.0032878055608535814	5.385393829890931E-4	6.788311550282687E-6	7.240865653634866E-5	1.018246732542403E-4
20	0.04049906670898651	5.679553997069848E-4	6.788311550282687E-6	7.693419756987045E-5	1.0861298480452299E-4
21	0.04129556193088634	5.883203343578329E-4	6.788311550282687E-6	7.919696808663134E-5	1.1540129635480567E-4
22	0.04149016019532778	6.109480395254418E-4	6.788311550282687E-6	8.598527963691403E-5	1.2671514893861015E-4
23	0.04168249568925246	6.539406793438988E-4	6.788311550282687E-6	8.824805015367493E-5	1.3350346048889285E-4
24	0.041886145035760936	6.72042843477986E-4	1.1313852583804478E-5	9.051082067043582E-5	1.4029177203917553E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	5340	0.0	11.151135	20
GATCGGA	5710	0.0	10.114155	21
CGGTTCA	6730	0.0	9.916002	1
AATGCCG	6760	0.0	9.768993	13
CGAGATC	6025	0.0	9.661561	18
GAGATCG	6320	0.0	9.253989	19
CCGAGAT	7465	0.0	8.316119	17
ATGCCGA	7930	0.0	8.160705	14
TGCCGAG	8370	0.0	7.951543	15
GCCGAGA	8645	0.0	7.6835375	16
ATCGGAA	7430	0.0	7.6762867	22
GAGCGGT	7280	0.0	7.424394	29
AGCGGTT	6910	0.0	7.3643227	30
AAGAGCG	8285	0.0	7.1962295	27
TCGGAAG	8665	0.0	6.9477925	23
TCAACGC	15875	0.0	6.7541275	9
GCGGTTC	6050	0.0	6.559817	11
CAACGCA	16400	0.0	6.478932	10
AGTGGTA	15875	0.0	6.3877654	2
GGTATCA	16550	0.0	6.3850904	5
>>END_MODULE