FastQCFastQC Report
Fri 27 May 2016
SRR522052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35326833
Sequences flagged as poor quality0
Sequence length36
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN663070.18769585147924242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG41400.010.05152920
GATCGGA43400.09.55529721
CGGTTCA53150.09.2593811
CGAGATC46300.09.15886618
GAGATCG48150.08.9229119
AATGCCG53450.08.7823213
AGCGGTT52950.07.469314630
GAGCGGT54800.07.38136429
CCGAGAT58700.07.362962217
TCAACGC123800.07.23318679
ATGCCGA65750.07.1122114
ATCGGAA59050.07.048805722
ATCAACG132850.06.85798748
TGCCGAG70000.06.85491815
GGTATCA128000.06.85066565
GCCGAGA68300.06.78637816
GTATCAA128550.06.68721256
AAGCAGT101400.06.6826631
AGTGGTA126100.06.6768582
CAACGCA130200.06.651795410
Produced by FastQC (version 0.11.5)