##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522052_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35326833
Sequences flagged as poor quality	0
Sequence length	36
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.47367201016859	35.0	26.0	39.0	2.0	39.0
2	27.25296436847311	35.0	16.0	39.0	2.0	39.0
3	26.90903738809533	35.0	11.0	39.0	2.0	39.0
4	26.705874908175325	35.0	8.0	39.0	2.0	39.0
5	26.518392463881494	35.0	2.0	39.0	2.0	39.0
6	25.863867502643103	34.0	2.0	39.0	2.0	39.0
7	25.6043577979379	34.0	2.0	38.0	2.0	39.0
8	25.40235732424698	33.0	2.0	38.0	2.0	39.0
9	25.189696710146645	33.0	2.0	38.0	2.0	39.0
10	24.98728801984599	33.0	2.0	38.0	2.0	39.0
11	24.814675235677083	33.0	2.0	38.0	2.0	39.0
12	24.59606495153415	33.0	2.0	38.0	2.0	39.0
13	24.355660554117602	33.0	2.0	38.0	2.0	39.0
14	24.333272274930504	33.0	2.0	38.0	2.0	39.0
15	24.15853025942065	33.0	2.0	38.0	2.0	39.0
16	23.93391391750288	33.0	2.0	38.0	2.0	39.0
17	23.671646847029848	32.0	2.0	38.0	2.0	39.0
18	23.581643449329295	32.0	2.0	38.0	2.0	39.0
19	23.4065390180886	32.0	2.0	38.0	2.0	39.0
20	23.199590690736414	31.0	2.0	38.0	2.0	39.0
21	22.966654016226137	31.0	2.0	38.0	2.0	39.0
22	22.735599367200564	31.0	2.0	38.0	2.0	39.0
23	22.50197253741936	31.0	2.0	38.0	2.0	39.0
24	22.331034768953106	30.0	2.0	37.0	2.0	39.0
25	21.93648884404668	30.0	2.0	37.0	2.0	39.0
26	21.773153511949403	29.0	2.0	36.0	2.0	39.0
27	21.536015640009396	29.0	2.0	36.0	2.0	39.0
28	21.349601363926396	29.0	2.0	36.0	2.0	39.0
29	21.20540624176529	29.0	2.0	36.0	2.0	39.0
30	20.741499499827793	27.0	2.0	36.0	2.0	38.0
31	20.776061669609614	29.0	2.0	36.0	2.0	39.0
32	20.503066125401052	27.0	2.0	36.0	2.0	38.0
33	20.25573931294662	27.0	2.0	35.0	2.0	38.0
34	19.95013478847651	27.0	2.0	35.0	2.0	38.0
35	19.632346324393133	26.0	2.0	35.0	2.0	38.0
36	19.62164032649063	26.0	2.0	35.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-26.2559551108822
1	2	-25.0425470309554
1	3	-24.69450813624278
1	4	-24.500305118785043
1	5	-24.31456064223731
1	6	-23.663544810849363
1	7	-23.402255438373146
1	8	-23.202708945981474
1	9	-22.99850534363398
1	10	-22.799068140975415
1	11	-22.622280049044956
1	12	-22.407394205184314
1	13	-22.159254923067103
1	14	-22.14375200641779
1	15	-21.96696207882427
1	16	-21.74390526948457
1	17	-21.48587020751349
1	18	-21.3917085440305
1	19	-21.22216962694307
1	20	-21.01511434227623
1	21	-20.781049532614436
1	22	-20.5554195385001
1	23	-20.325389207637947
1	24	-20.155518164933955
1	25	-19.75605777242327
1	26	-19.602428577451942
1	27	-19.371182525092866
1	28	-19.19021751032417
1	29	-19.04050222988737
1	30	-18.576377001690393
1	31	-18.616666594244727
1	32	-18.352074783528757
1	33	-18.1070218886245
1	34	-17.801217907484666
1	35	-17.484554496633105
1	36	-17.473958700569685
2	1	-0.5268282981700771
2	2	-0.39567878041544446
2	3	-0.49448889723669254
2	4	-0.3655661375437269
2	5	-0.35567068431730675
2	6	-0.24508747823212218
2	7	-0.2652942275383374
2	8	-0.18162281168361716
2	9	-0.18831090942868656
2	10	-0.14664050478288715
2	11	-0.08381233755257256
2	12	-0.026748153673416653
2	13	0.005994645876686633
2	14	-0.1898569576838156
2	15	-0.06271220133373845
2	16	0.0012632847627642718
2	17	-0.0723133068202273
2	18	-0.05061280681495006
2	19	-0.02112325720668906
2	20	-0.06071755116237654
2	21	0.01648209342690876
2	22	0.0490852439744458
2	23	0.12107793213372986
2	24	-0.12938425564669842
2	25	0.29914124022714716
2	26	0.14317463143420284
2	27	-0.03610076020548547
2	28	0.16954799414975597
2	29	0.05706025082846011
2	30	0.09357054536298648
2	31	0.0885169910684489
2	32	0.07578698752955759
2	33	-0.03278554181796167
2	34	0.12892710948540298
2	35	-0.0274424941647311
2	36	0.07800057125999516
3	1	-0.5559085342636649
3	2	-0.5580880960074097
3	3	-0.4326917506497807
3	4	-0.43767238607757974
3	5	-0.5639692217118437
3	6	-0.6096561132603142
3	7	-0.6297933641008591
3	8	-0.549188867441142
3	9	-0.6104098885993139
3	10	-0.6274975289350415
3	11	-0.46648364147418064
3	12	-0.5483219087764013
3	13	-0.50440557340659
3	14	-0.4867911833835805
3	15	-0.42436237983911695
3	16	-0.5522846794538623
3	17	-0.3763046063864479
3	18	-0.32643479455250457
3	19	-0.3756507291961668
3	20	-0.27898641183064754
3	21	-0.4332463964554556
3	22	-0.359735638484576
3	23	-0.3399521637006693
3	24	-0.28652266734041376
3	25	-0.3276149840363729
3	26	-0.25288658086756044
3	27	-0.1968617674465385
3	28	-0.21605200807296754
3	29	-0.29179861243666494
3	30	-0.02183578138298614
3	31	-0.3351575118041126
3	32	-0.3156829189114596
3	33	-0.31664772312225153
3	34	-0.3809105018023189
3	35	-0.20863460841698966
3	36	-0.21788045185054372
4	1	-0.5962029051378792
4	2	-0.8554811402101414
4	3	-0.5744279798649146
4	4	-0.6832936549533706
4	5	-0.5866023806094596
4	6	-0.6310117595364737
4	7	-0.6004779628676715
4	8	-0.8327112599935234
4	9	-0.7595069693286511
4	10	-0.7186998873947701
4	11	-0.9499430546041232
4	12	-0.7696295153552093
4	13	-0.5980811849932053
4	14	-0.9777350169829049
4	15	-0.6818661831882693
4	16	-0.6498744931844023
4	17	-0.38319281641065217
4	18	-0.4805198927538008
4	19	-0.6046421983683246
4	20	-0.5369527754155854
4	21	-0.29800716980860287
4	22	-0.3653187938658462
4	23	-0.24707746244052586
4	24	-0.27475350690254174
4	25	-0.026097194236673005
4	26	-0.25054196209620727
4	27	-0.19944733956004512
4	28	-0.16853551295229252
4	29	-0.2306029745216236
4	30	-0.2675102223899728
4	31	-0.34424934876572166
4	32	-0.4404609921514968
4	33	-0.4270268945690603
4	34	-0.590624077311336
4	35	-0.640489669651064
4	36	-0.47818246629147865
5	1	-0.3737503071550634
5	2	-0.27788558983725764
5	3	-0.3317364967091194
5	4	-0.33297715004297146
5	5	-0.2611037003458563
5	6	-0.2487404251798857
5	7	-0.2573053708088757
5	8	-0.1445332230717966
5	9	-0.21369506284978002
5	10	-0.40948241659862106
5	11	-0.1358983828837772
5	12	-0.2624045496001486
5	13	-0.14820929745114242
5	14	-0.10081794311492587
5	15	-0.1453583268507721
5	16	0.01570781315823666
5	17	0.1140588690876072
5	18	-0.04604704636581758
5	19	0.10491913801157082
5	20	0.167150271946074
5	21	0.163421144503328
5	22	0.0028956942886431136
5	23	-0.08063836556913273
5	24	0.11973602967050923
5	25	0.22071057208518496
5	26	0.07865556647099581
5	27	0.13874916502581058
5	28	0.3148663817651034
5	29	0.10697785951358085
5	30	0.23317379001168703
5	31	0.08705110021807982
5	32	0.2170692968061836
5	33	0.2507707893117228
5	34	0.13783834586000054
5	35	0.2462353166951914
5	36	0.15880485909915265
6	1	-0.34984350769353867
6	2	-0.31130573018354113
6	3	-0.20474799451472947
6	4	-0.2618938710314893
6	5	-0.19987958440145093
6	6	-0.29838901038944243
6	7	-0.25779126710201083
6	8	-0.2091549515140514
6	9	-0.2628597383858384
6	10	-0.3851063496967413
6	11	-0.23633787054725985
6	12	-0.46425855585275855
6	13	-0.2506947514022535
6	14	-0.3898799278821343
6	15	-0.32548402787307396
6	16	-0.37620308733149344
6	17	-0.20856779530083003
6	18	-0.15458968466623801
6	19	-0.16321095991083112
6	20	-0.19615318828407524
6	21	0.14119518458156577
6	22	0.10002775999414126
6	23	0.11188144907469422
6	24	-0.13821460932216212
6	25	0.30205307156002803
6	26	0.11850018377797156
6	27	0.19009167457814513
6	28	0.017994561041131618
6	29	0.1442693758319784
6	30	0.4590525820119993
6	31	0.25819105474932513
6	32	0.13448095479846245
6	33	-0.02259192531942844
6	34	0.20912626561415593
6	35	0.20777752917987868
6	36	0.1448569390608334
7	1	-0.7596449130324636
7	2	-0.5814171880900787
7	3	-0.6667250528184496
7	4	-0.6416047121877071
7	5	-0.7135222626745481
7	6	-0.6768552736347146
7	7	-0.7829413877393847
7	8	-0.6429274950871573
7	9	-0.6594122316925208
7	10	-0.6615474994035253
7	11	-0.7067324515046423
7	12	-0.7584030043613481
7	13	-0.5424222565020393
7	14	-0.7593132580409083
7	15	-0.7700352586870984
7	16	-2.4009446764498925
7	17	-10.416456201787984
7	18	-2.3832794097400196
7	19	-2.2367063301740586
7	20	-2.248208050527392
7	21	-1.335442305691604
7	22	-1.2607761133458268
7	23	-1.3150832085285877
7	24	-1.1976646119089551
7	25	-1.065015085229426
7	26	-0.3127473000169907
7	27	-0.20215141624370148
7	28	-0.20074616990811478
7	29	-0.33835832760350826
7	30	0.0073433367535287175
7	31	-0.4890503336683558
7	32	-0.4294333941273969
7	33	-0.4584119261586963
7	34	-0.42902501989639674
7	35	-0.5444183556572177
7	36	-0.43811036416151694
8	1	-0.3940388211453083
8	2	-0.2927859301081064
8	3	-0.01914081308749971
8	4	-0.0387243830648103
8	5	-0.2703648805268912
8	6	-0.17738164308579485
8	7	-0.09251331835170262
8	8	-0.031196718594806327
8	9	-0.018462823834749287
8	10	-0.09659543950324334
8	11	-0.07045064381276589
8	12	-0.24283625309603352
8	13	-0.013299251669401713
8	14	-0.10100076898836363
8	15	-0.07471730759857564
8	16	0.07829145969785856
8	17	0.07809986217577958
8	18	0.12979378494806681
8	19	0.013450049299308375
8	20	0.15870868123144533
8	21	0.16135984497363864
8	22	0.08505316329005197
8	23	0.14970157404007978
8	24	0.07255089284778649
8	25	0.39986374124176294
8	26	0.2839191323015129
8	27	0.015165184660588693
8	28	0.23801718420344642
8	29	0.19486121535694423
8	30	0.1884825256078173
8	31	-0.22656020448872383
8	32	0.006114679425948566
8	33	-0.20070220970496422
8	34	-0.12739488397699006
8	35	-0.039278329212578456
8	36	-0.3806924713539104
9	1	-0.4285625092587928
9	2	-0.3678381856283117
9	3	-0.13441086655784673
9	4	-0.17716091815119128
9	5	-0.16493531582935006
9	6	-0.34252336361135605
9	7	-0.3203055957767944
9	8	-0.21940219514824122
9	9	-0.2522931418719381
9	10	-0.2442635455558495
9	11	-0.10972321592048218
9	12	-0.2394425887815821
9	13	-0.14023333329339494
9	14	-0.0646386588077732
9	15	-0.1535935417631933
9	16	-0.26264842313448966
9	17	-0.26695156840437306
9	18	0.002538773621189705
9	19	0.03745675323870046
9	20	0.03583371508845801
9	21	0.1522174955224962
9	22	0.1688257051714963
9	23	0.24482478703624722
9	24	0.2555312752149099
9	25	0.3938668321517902
9	26	0.07459025536271469
9	27	0.296050944059175
9	28	0.16388432162423783
9	29	0.5184184926253828
9	30	0.31681816016914865
9	31	-0.3822991137352467
9	32	-0.037563570464495655
9	33	0.022882824391636802
9	34	0.06868488054189825
9	35	0.157012444879296
9	36	0.10668232095811447
10	1	-0.1827608893733732
10	2	-0.4323704659473755
10	3	-0.009381720578080177
10	4	-0.08813497986940533
10	5	-0.22782909013601227
10	6	-0.11231066153315439
10	7	-0.06128221848301152
10	8	-0.13860917669094874
10	9	-0.06846656952804508
10	10	-0.022832899278967034
10	11	0.07444378398017903
10	12	-0.022548381789999183
10	13	0.09470807528708391
10	14	-0.2694037753188212
10	15	0.10908759689749203
10	16	0.15928303868806637
10	17	0.1601487921990099
10	18	0.09834974809077224
10	19	-1.3701700478208068E-4
10	20	0.16639448597416973
10	21	0.02774661505811693
10	22	0.00990951446940258
10	23	0.09794947615017335
10	24	0.18303721227310632
10	25	0.16333227573082354
10	26	-0.04268539928179749
10	27	0.22480463381564064
10	28	0.156491959980805
10	29	0.20306117300187765
10	30	0.5098830304123361
10	31	0.4157892645032675
10	32	0.2665769018645001
10	33	0.27397329596619
10	34	0.2420244681172612
10	35	0.30902495762050464
10	36	0.40129009557798767
11	1	-0.13989230117758567
11	2	-0.20655372379277637
11	3	-0.014260093712028521
11	4	-0.1375350261672459
11	5	-0.006408616181232674
11	6	-0.0817814003576558
11	7	-0.059556552713964095
11	8	-0.2645281604275169
11	9	0.019764250723632415
11	10	-0.12901806564491736
11	11	0.15132790209565883
11	12	0.09467117564933147
11	13	0.2973763886501253
11	14	0.2081750128739266
11	15	0.25014287984578587
11	16	0.366507111251515
11	17	0.3760435392947272
11	18	0.27050897217118575
11	19	0.3955469561226366
11	20	0.37020646343842145
11	21	0.29189595937346624
11	22	0.269794439233344
11	23	0.3073735184061981
11	24	0.11087606892922608
11	25	0.4587565656937258
11	26	0.3106932450591593
11	27	0.3393844354580047
11	28	0.3093320102369965
11	29	0.26698216421778653
11	30	0.43745915250228506
11	31	0.22106427646767557
11	32	0.18618379166913357
11	33	0.08732781212844287
11	34	0.12393815146378628
11	35	0.14383214343382278
11	36	0.3352954341880192
12	1	-0.014984218193102805
12	2	-0.14249186659806412
12	3	0.05364934600245164
12	4	-0.2172010052662614
12	5	-0.01144972519959353
12	6	0.13732496983934794
12	7	0.01639941384860677
12	8	0.006041192695651176
12	9	-0.017356853397650696
12	10	-0.06044540297772727
12	11	0.1285087929173656
12	12	0.009423127546089205
12	13	0.23215435566713083
12	14	0.1200004595183124
12	15	0.16595351136143677
12	16	0.18394905056338118
12	17	0.30926838186911354
12	18	0.3082520809789955
12	19	0.18830792054427903
12	20	0.3130811342464668
12	21	0.2123956437492609
12	22	0.18384774903600132
12	23	0.14013306902753087
12	24	0.21787963498167429
12	25	0.5366702672950687
12	26	0.0594788113269793
12	27	0.050078467216572164
12	28	0.276505402353898
12	29	0.09536433726712445
12	30	0.38176298597874947
12	31	0.017236770781071442
12	32	-0.07521460894978915
12	33	-0.0744424681747482
12	34	-0.05604478875149965
12	35	0.0028712748566555035
12	36	-0.250380805252167
13	1	-0.026906100820532686
13	2	-0.06439058824199506
13	3	0.057389787911535706
13	4	-0.008308163723430795
13	5	-0.05136768400315006
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118	8	-0.5530433052625661
118	9	-0.3446939198570931
118	10	-0.2921289723113354
118	11	-0.46987510868723703
118	12	-0.2596610054367048
118	13	-0.2637957162654736
118	14	-0.25308829494442264
118	15	-0.15497542941389142
118	16	-0.26976967059173873
118	17	-0.29781077854493887
118	18	-0.26009506629354107
118	19	-0.17826730569394655
118	20	-0.3208503734624202
118	21	-0.1310897528978323
118	22	-0.2371224034218251
118	23	-0.155535856978954
118	24	-0.07269531981298627
118	25	0.14812513705015462
118	26	-0.2573385458944877
118	27	-0.18047650442582963
118	28	-0.22152745401577434
118	29	-0.2846702887946151
118	30	-0.20427750289699986
118	31	-0.6732534391181879
118	32	-0.5053450019558348
118	33	-0.35827490514575544
118	34	-0.39988135652865964
118	35	-0.3929760052031206
118	36	-0.432036789796836
119	1	-0.3840274369624943
119	2	-0.46154795035472773
119	3	-0.3491989819683745
119	4	-0.4393587746519998
119	5	-0.4167231398170266
119	6	-0.3618930161497538
119	7	-0.4744691103770968
119	8	-0.5228384726199629
119	9	-0.438892302083687
119	10	-0.4285836362987858
119	11	-0.3947898974196704
119	12	-0.4457330452844346
119	13	-0.11521558357788919
119	14	-0.33014478461169006
119	15	-0.550075666089576
119	16	-0.3232596095853637
119	17	-0.24752558991200857
119	18	-0.31087287493730287
119	19	-0.24013873872636537
119	20	-0.34756913847604665
119	21	-0.2587081289981299
119	22	-0.20007169908851452
119	23	-0.28937940071180535
119	24	-0.5074948734843687
119	25	-0.031142050694796808
119	26	-0.14138046222056033
119	27	-0.23440655206191252
119	28	-0.2928224750805306
119	29	-0.34791871778194405
119	30	-0.35538405041855725
119	31	-0.10281431106973571
119	32	-0.35363776238257216
119	33	-0.39381318497754947
119	34	-0.28647687162809277
119	35	-0.30098109792026406
119	36	-0.35314962916300274
120	1	-26.2559551108822
120	2	-25.02982215162236
120	3	-24.69535282812222
120	4	-24.50126499592077
120	5	-24.31540533411675
120	6	-23.66492703392481
120	7	-23.403368895850587
120	8	-23.20374561328806
120	9	-22.999542010940566
120	10	-22.800296783709143
120	11	-22.625620421477286
120	12	-22.410734577616644
120	13	-22.162595295499433
120	14	-22.14709237885012
120	15	-21.9703024512566
120	16	-21.745018726962012
120	17	-21.48675329447836
120	18	-21.392822001507945
120	19	-21.223283084420512
120	20	-21.01511434227623
120	21	-20.781049532614436
120	22	-20.5554195385001
120	23	-20.325389207637947
120	24	-20.155518164933955
120	25	-19.75605777242327
120	26	-19.602428577451942
120	27	-19.371182525092866
120	28	-19.19021751032417
120	29	-19.04050222988737
120	30	-18.576377001690393
120	31	-18.616666594244727
120	32	-18.352074783528757
120	33	-18.1070218886245
120	34	-17.801217907484666
120	35	-17.484554496633105
120	36	-17.473958700569685
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	8013406.0
3	469904.0
4	418187.0
5	489399.0
6	500816.0
7	205167.0
8	237316.0
9	312236.0
10	238838.0
11	175817.0
12	197382.0
13	225225.0
14	211665.0
15	189135.0
16	196194.0
17	209605.0
18	211991.0
19	216128.0
20	234272.0
21	266040.0
22	299664.0
23	335137.0
24	374248.0
25	420389.0
26	472463.0
27	532158.0
28	606776.0
29	708448.0
30	847526.0
31	1031912.0
32	1271592.0
33	1597242.0
34	2031902.0
35	2552739.0
36	3027249.0
37	3072421.0
38	2094040.0
39	832058.0
40	146.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.44781336041569	29.609718740811196	23.22488367626686	27.717584222506254
2	25.31206098957296	29.472296737513876	24.01961019561152	21.196032077301652
3	24.02855182976184	27.390847650717227	24.676915284264147	23.903685235256788
4	26.87950275666508	26.150715129755493	23.305839895350488	23.663942218228943
5	26.775832785219205	27.532642107243188	24.248571015485858	21.442954092051746
6	25.999212940412903	26.355886506375615	22.04257912617369	25.6023214270378
7	24.7727558043881	25.868085492237654	25.214750427455325	24.14440827591892
8	24.66730161194345	25.54266183757759	24.901441299325693	24.888595251153262
9	23.962246055702106	25.23667218318752	25.160508297194323	25.64057346391605
10	24.22399778037911	26.60438842742042	23.50858154690742	25.66303224529305
11	24.581461423268674	25.851867492924796	24.09113079019967	25.47554029360686
12	24.377984257968897	25.487850704221128	24.676716324919283	25.457448712890695
13	24.132785871974637	25.675945238582408	24.7324743879311	25.458794501511857
14	24.12704037284288	25.804528614112503	24.875346679849887	25.193084333194726
15	23.7690662483155	25.467112592799324	25.041381240287276	25.722439918597896
16	23.927778201822775	25.378008959590346	24.86354002205619	25.830672816530686
17	23.913206202909485	25.173504507603926	24.768429162156718	26.144860127329867
18	23.75007341333574	25.185725930054755	24.830912333380763	26.233288323228738
19	24.087152213000635	25.169870325380412	24.757899789407624	25.985077672211332
20	24.01066839632802	25.19288409390374	24.76453076937608	26.031916740392163
21	23.882974780398207	25.121939518803458	24.674719492108395	26.32036620868994
22	23.962237388668214	25.02907972855166	24.52156977088568	26.487113111894438
23	23.97081833449699	24.96796010665112	24.487934409284684	26.573287149567204
24	24.033860156739543	24.91038417642354	24.434250896362776	26.621504770474143
25	24.126755752440683	24.785739183412076	24.23023611374997	26.857268950397273
26	24.26501857167585	24.896096892074755	24.366573395520536	26.47231114072886
27	24.28079569633825	24.6729219967923	24.25397246812194	26.792309838747506
28	24.17279995768226	24.77085290082756	24.197412887326184	26.858934254164
29	24.166144526802718	24.914102008871716	24.44571294828762	26.474040516037945
30	23.937833168715983	24.62563553576677	24.31176617300392	27.124765122513327
31	24.087254670693696	24.725314535054764	24.32245691181358	26.86497388243796
32	24.111157993414324	24.719949638011393	24.34821339837426	26.820678970200024
33	24.03445049476751	24.555141430123875	24.26833348291278	27.142074592195836
34	24.118548180915706	24.511491386273303	24.087379417184447	27.28258101562654
35	24.059457869891446	24.578091993138447	24.186602530061514	27.175847606908594
36	24.086857162164392	24.471412674017987	24.092565137182557	27.349165026635063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	797280.0
1	962259.5
2	1127239.0
3	1127239.0
4	896541.5
5	665844.0
6	665844.0
7	523975.0
8	382106.0
9	382106.0
10	304100.5
11	226095.0
12	226095.0
13	186773.0
14	147451.0
15	139094.5
16	130738.0
17	130738.0
18	148300.0
19	165862.0
20	165862.0
21	208731.0
22	251600.0
23	251600.0
24	313295.0
25	374990.0
26	467102.5
27	559215.0
28	559215.0
29	694528.5
30	829842.0
31	829842.0
32	983686.0
33	1137530.0
34	1137530.0
35	1307923.5
36	1478317.0
37	1478317.0
38	1649820.0
39	1821323.0
40	2004647.5
41	2187972.0
42	2187972.0
43	2371963.0
44	2555954.0
45	2555954.0
46	2692007.5
47	2828061.0
48	2828061.0
49	2899499.5
50	2970938.0
51	2964983.5
52	2959029.0
53	2959029.0
54	2863737.0
55	2768445.0
56	2768445.0
57	2611902.5
58	2455360.0
59	2455360.0
60	2234321.5
61	2013283.0
62	2013283.0
63	1774898.5
64	1536514.0
65	1314284.5
66	1092055.0
67	1092055.0
68	911034.5
69	730014.0
70	730014.0
71	597397.5
72	464781.0
73	464781.0
74	376123.0
75	287465.0
76	229603.0
77	171741.0
78	171741.0
79	135911.5
80	100082.0
81	100082.0
82	77891.5
83	55701.0
84	55701.0
85	42321.5
86	28942.0
87	28942.0
88	21403.5
89	13865.0
90	9813.5
91	5762.0
92	5762.0
93	4009.5
94	2257.0
95	2257.0
96	1730.0
97	1203.0
98	1203.0
99	1590.0
100	1977.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.96926794994615
2	3.2648978186071758
3	4.5258430043814
4	5.8528427951636655
5	6.8634004072768136
6	7.712191466469695
7	8.250595234506303
8	8.684291059999632
9	9.00268642818902
10	9.1977760927508
11	9.362916851335076
12	9.423230777579185
13	9.605318993638631
14	9.522466958756253
15	9.608812089099523
16	9.658326858793144
17	9.920518490859342
18	9.821265325425577
19	9.864314754736151
20	9.940721264201633
21	9.956202414181876
22	10.013651662462921
23	10.042510745302303
24	10.043713796818412
25	10.921046899392312
26	10.104506112959518
27	10.139485189629085
28	10.097432170044792
29	10.11381065492058
30	10.281470744915062
31	10.179989811144406
32	10.135830743729562
33	10.203462053901067
34	10.162051605361851
35	10.170458812427368
36	10.188555537939108
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3.5326833E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.65036985823342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.94647758349822	99.59703457223232
2	0.028084386698424727	0.055972390434793504
3	0.011383662735324925	0.03403158605749554
4	0.005029345997409789	0.02004704755147645
5	8.058391362693238E-4	0.004015108398773873
6	0.002185581649635547	0.01306764118449301
7	0.0015873980805244101	0.011072936408555509
8	7.586971414052451E-4	0.006048356060113368
9	5.627951739485514E-4	0.005047447251456175
>10	0.0029224276758962704	0.04815297062779592
>50	1.6940181425349548E-4	0.012178640005735656
>100	3.003308740102175E-5	0.005194720467904169
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.8473113456482087E-6	0.18813658331905111
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	66307	0.18769585147924242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8122060644383264E-5	3.9629932295374455E-5	2.830709449669604E-6	2.830709449669604E-6	2.830709449669604E-6
2	6.510631734240089E-5	7.35984456914097E-5	2.830709449669604E-6	8.492128349008812E-6	5.661418899339208E-6
3	7.07677362417401E-5	9.907483073843614E-5	2.830709449669604E-6	8.492128349008812E-6	1.415354724834802E-5
4	7.925986459074891E-5	1.330433441344714E-4	2.830709449669604E-6	1.1322837798678415E-5	1.9814966147687228E-5
5	8.775199293975771E-5	1.556890197318282E-4	2.830709449669604E-6	1.9814966147687228E-5	2.5476385047026434E-5
6	9.624412128876653E-5	1.6984256698017623E-4	2.830709449669604E-6	2.264567559735683E-5	3.679922284570485E-5
7	1.0190554018810573E-4	1.8965753312786345E-4	2.830709449669604E-6	2.264567559735683E-5	4.529135119471366E-5
8	1.1322837798678416E-4	2.236260465238987E-4	2.830709449669604E-6	2.5476385047026434E-5	4.529135119471366E-5
9	1.35874053584141E-4	2.6042526936960356E-4	2.830709449669604E-6	3.113780394636564E-5	4.8122060644383264E-5
10	7.727836797598018E-4	2.802402355172908E-4	2.830709449669604E-6	4.246064174504406E-5	5.944489844306168E-5
11	9.256419900419605E-4	3.0854733001398684E-4	2.830709449669604E-6	4.529135119471366E-5	6.510631734240089E-5
12	9.964097262837005E-4	3.3119300561134366E-4	2.830709449669604E-6	5.095277009405287E-5	7.07677362417401E-5
13	0.0010332089491294054	3.708229379067181E-4	2.830709449669604E-6	6.79370267920705E-5	7.64291551410793E-5
14	0.001086992428673128	3.8214577570539653E-4	2.830709449669604E-6	7.07677362417401E-5	7.925986459074891E-5
15	0.002694835396085463	3.9629932295374455E-4	2.830709449669604E-6	7.07677362417401E-5	8.20905740404185E-5
16	0.002850524415817291	4.2177570800077096E-4	2.830709449669604E-6	7.925986459074891E-5	9.624412128876653E-5
17	0.003037351239495485	4.415906741484582E-4	2.830709449669604E-6	8.775199293975771E-5	1.0756695908744494E-4
18	0.0032326701915226875	4.8122060644383263E-4	2.830709449669604E-6	9.341341183909693E-5	1.0756695908744494E-4
19	0.0035780167443823794	5.095277009405287E-4	2.830709449669604E-6	9.907483073843614E-5	1.0756695908744494E-4
20	0.03458277734661355	5.43496214336564E-4	2.830709449669604E-6	1.0190554018810573E-4	1.1322837798678416E-4
21	0.03545180514766212	5.746340182829296E-4	2.830709449669604E-6	1.0190554018810573E-4	1.1888979688612337E-4
22	0.03573770680207875	5.916182749809472E-4	8.492128349008812E-6	1.0756695908744494E-4	1.415354724834802E-4
23	0.03600945490924703	6.086025316789648E-4	8.492128349008812E-6	1.0756695908744494E-4	1.528583102821586E-4
24	0.036391600684952424	6.255867883769824E-4	8.492128349008812E-6	1.1322837798678416E-4	1.6135043863116743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	4140	0.0	10.051529	20
GATCGGA	4340	0.0	9.555297	21
CGGTTCA	5315	0.0	9.259381	1
CGAGATC	4630	0.0	9.158866	18
GAGATCG	4815	0.0	8.92291	19
AATGCCG	5345	0.0	8.78232	13
AGCGGTT	5295	0.0	7.4693146	30
GAGCGGT	5480	0.0	7.381364	29
CCGAGAT	5870	0.0	7.3629622	17
TCAACGC	12380	0.0	7.2331867	9
ATGCCGA	6575	0.0	7.11221	14
ATCGGAA	5905	0.0	7.0488057	22
ATCAACG	13285	0.0	6.8579874	8
TGCCGAG	7000	0.0	6.854918	15
GGTATCA	12800	0.0	6.8506656	5
GCCGAGA	6830	0.0	6.786378	16
GTATCAA	12855	0.0	6.6872125	6
AAGCAGT	10140	0.0	6.682663	1
AGTGGTA	12610	0.0	6.676858	2
CAACGCA	13020	0.0	6.6517954	10
>>END_MODULE