FastQCFastQC Report
Fri 27 May 2016
SRR522051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41097635
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3029250.7370862094619313No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2272900.5530488554876698No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1536720.37391932650139115No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1093350.2660372062771982No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1046290.254586425715251No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN793040.1929648749861154No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN792440.19281888118379562No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN748860.1822148646753031No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN685850.16688308220168874No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN548600.13348699992104168No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN534280.13000261450567654No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN482880.11749581210694972No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN474660.11549569701516887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG56600.010.94920520
CGGTTCA73700.09.630681
CGAGATC65050.09.52008318
GAGATCG66100.09.46816119
GATCGGA66500.09.10337521
AATGCCG76350.09.002007513
CCGAGAT77600.08.22721317
ATGCCGA85300.07.919640514
ATCGGAA81200.07.568024622
TGCCGAG88900.07.45243415
AGCGGTT72500.07.371444730
TCGGAAG89800.07.360346323
GCGGTTC66000.07.347143711
GAGCGGT73700.07.227033629
GCCGAGA88100.07.17762916
AGTGGTA136900.06.67901372
TCAACGC140150.06.6198469
AAGAGCG88850.06.586426327
CAACGCA144250.06.479137410
AAGCAGT121800.06.34269861