##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522051_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41097635
Sequences flagged as poor quality	0
Sequence length	36
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.958716651213628	33.0	15.0	38.0	2.0	39.0
2	25.612340539790186	33.0	2.0	38.0	2.0	39.0
3	25.355817092638055	33.0	2.0	38.0	2.0	39.0
4	25.08607940578576	33.0	2.0	38.0	2.0	39.0
5	24.752859063544655	33.0	2.0	38.0	2.0	39.0
6	24.25718995752432	33.0	2.0	38.0	2.0	39.0
7	24.041644245465708	33.0	2.0	38.0	2.0	39.0
8	23.803953268843816	32.0	2.0	38.0	2.0	39.0
9	23.21399525787798	31.0	2.0	38.0	2.0	39.0
10	23.33532973369392	31.0	2.0	38.0	2.0	39.0
11	23.231502080350854	32.0	2.0	38.0	2.0	39.0
12	23.03833605023744	31.0	2.0	38.0	2.0	39.0
13	22.885591908147514	31.0	2.0	38.0	2.0	39.0
14	22.814516163764655	31.0	2.0	38.0	2.0	39.0
15	22.564823547632365	31.0	2.0	38.0	2.0	39.0
16	22.545106962967576	31.0	2.0	38.0	2.0	39.0
17	22.310765960133715	31.0	2.0	38.0	2.0	39.0
18	22.075095635065132	30.0	2.0	38.0	2.0	39.0
19	21.964909270326626	30.0	2.0	38.0	2.0	39.0
20	21.852353766828674	30.0	2.0	38.0	2.0	39.0
21	21.637271609424726	29.0	2.0	37.0	2.0	39.0
22	21.403454797338096	29.0	2.0	37.0	2.0	39.0
23	21.260557231577923	29.0	2.0	36.0	2.0	39.0
24	21.065232415441912	29.0	2.0	36.0	2.0	39.0
25	20.891184492732975	28.0	2.0	36.0	2.0	39.0
26	20.550388994403207	27.0	2.0	36.0	2.0	39.0
27	20.292484689204134	27.0	2.0	36.0	2.0	38.0
28	20.050029618492644	26.0	2.0	35.0	2.0	38.0
29	19.83875648805582	26.0	2.0	35.0	2.0	38.0
30	19.71528602558274	26.0	2.0	35.0	2.0	38.0
31	19.36819588280445	24.0	2.0	35.0	2.0	38.0
32	19.087536861914316	23.0	2.0	35.0	2.0	38.0
33	18.539417535826576	20.0	2.0	35.0	2.0	38.0
34	18.528167423745916	21.0	2.0	35.0	2.0	38.0
35	18.23942621029166	19.0	2.0	35.0	2.0	38.0
36	18.156515526988354	18.0	2.0	35.0	2.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-24.59629141619777
1	2	-23.272987578129364
1	3	-23.021033771493897
1	4	-22.757253730716837
1	5	-22.452117315926763
1	6	-21.935902532757446
1	7	-21.720442893180646
1	8	-21.48871018265019
1	9	-20.908505657465557
1	10	-21.029679285087393
1	11	-20.92432898337995
1	12	-20.73753499272033
1	13	-20.58544320897261
1	14	-20.513867220809686
1	15	-20.263577609218114
1	16	-20.251315217669244
1	17	-20.019702984225297
1	18	-19.782237405318615
1	19	-19.684878451293688
1	20	-19.56867411815618
1	21	-19.35971215719945
1	22	-19.132475505577553
1	23	-18.98553646605825
1	24	-18.79621952171068
1	25	-18.6238508451205
1	26	-18.29378299030748
1	27	-18.036010595013025
1	28	-17.80064499127167
1	29	-17.594141250960398
1	30	-17.470829218745966
1	31	-17.124125031523263
1	32	-16.847756316057023
1	33	-16.27434913523007
1	34	-16.2953788042978
1	35	-16.01243382259995
1	36	-15.923537290552932
2	1	-0.9425737887959293
2	2	-0.9569370351051347
2	3	-0.8690914156041742
2	4	-0.989135518519678
2	5	-0.9561638906970238
2	6	-1.27377847260707
2	7	-1.1216020410503198
2	8	-1.1766540005282202
2	9	-0.9419747468556992
2	10	-1.5187404797406927
2	11	-1.2754672457024228
2	12	-1.249335326889085
2	13	-1.461017561520645
2	14	-1.1701172208096864
2	15	-1.166094317655876
2	16	-1.1348260781537896
2	17	-1.4888706951693251
2	18	-1.2880331446669864
2	19	-1.4977961622377158
2	20	-1.3899199410467418
2	21	-1.9173562674751388
2	22	-4.561203575752991
2	23	-1.7345705095001875
2	24	-1.8609647180348219
2	25	-1.8048502185540833
2	26	-1.558742263490437
2	27	-1.569531898271169
2	28	-1.5522114073117699
2	29	-1.570122871678862
2	30	-1.5566427108094558
2	31	-1.1900711468114835
2	32	-1.1578283711948671
2	33	-0.7243021427488685
2	34	-0.845060299702979
2	35	-0.9404570055573416
2	36	-0.9143737567183443
3	1	-0.5525409229195084
3	2	-0.3719589285279312
3	3	-0.5131281652220281
3	4	-0.2374568298197275
3	5	-0.3558557074450093
3	6	-0.46788011819323927
3	7	-0.4870104657254366
3	8	-0.5124138473788236
3	9	-0.19686955212763024
3	10	-0.7203477757874523
3	11	-0.7752142534333579
3	12	-0.6767894190705981
3	13	-0.5520581362305101
3	14	-0.6417775625528677
3	15	-0.8134184447657198
3	16	-0.691372043324975
3	17	-0.7137389211909131
3	18	-0.5270481180070483
3	19	-0.590642572206054
3	20	-0.6399955777336714
3	21	-0.8900586358503659
3	22	-0.555958181645007
3	23	-0.5319572389924012
3	24	-0.6912235994776417
3	25	-0.7243743777820981
3	26	-0.701138755743802
3	27	-0.8362526302133624
3	28	-0.8750181990273198
3	29	-0.752148143701973
3	30	-0.7474070514785964
3	31	-0.5971986154858016
3	32	-0.6377118552430474
3	33	-0.26837717974350284
3	34	-0.6087091560380884
3	35	-0.5095793857264113
3	36	-0.5595637039595793
4	1	-0.373686681279068
4	2	-0.25842267160958343
4	3	-0.16339911231271032
4	4	-0.2837353789346224
4	5	-0.07419771421075083
4	6	-0.3047918924311901
4	7	-0.24978455940595268
4	8	-0.4869359122196002
4	9	0.019755557512461053
4	10	-0.2658955871900375
4	11	-0.17044942191604662
4	12	-0.2841162119354159
4	13	-0.3910679110540656
4	14	-0.3947792487743058
4	15	-0.3129923647924997
4	16	-0.45201962951183816
4	17	-0.23359525688034566
4	18	-0.14853156346701724
4	19	-0.4476823282069873
4	20	-0.19387935092986908
4	21	-0.29417007786043214
4	22	-0.20511466663893785
4	23	-0.40460854910469735
4	24	-0.2241894385583052
4	25	-0.29375710008580924
4	26	-0.47531468884039896
4	27	-0.3719832659520641
4	28	-0.2499061532863962
4	29	-0.2822403982513251
4	30	-0.1727517864065753
4	31	-0.25960514380545163
4	32	-0.1252310260829752
4	33	0.14715925945241182
4	34	-0.18600959715834975
4	35	-0.06656231155710302
4	36	-0.11409923112811526
5	1	-0.23513968674252794
5	2	-0.2639215637194532
5	3	-0.24848172831733706
5	4	-0.00408328432861893
5	5	0.0446258313893253
5	6	-0.08210198507446265
5	7	-0.16106182812335135
5	8	-0.1361724962119908
5	9	0.29583858521649375
5	10	-0.18762999361901933
5	11	-0.07002329119905681
5	12	-0.14936069128199847
5	13	0.02485110416380465
5	14	-0.12062552246070979
5	15	-0.126390998205963
5	16	-0.060822568851016
5	17	-0.14939218742631866
5	18	0.108305757949406
5	19	0.08501493663069937
5	20	-0.24913034339745366
5	21	-0.02131851670281293
5	22	-0.06757773087199936
5	23	-0.2973701405856737
5	24	-0.2777285744318121
5	25	-0.41921415036070897
5	26	-0.4268486856787632
5	27	-0.6271360037812705
5	28	-0.27681014693593653
5	29	-0.4966749494157199
5	30	-0.42382419388754045
5	31	-0.15065841093995402
5	32	-0.1563198446242744
5	33	0.06050849378117462
5	34	-0.12206106840861253
5	35	-0.2386587980073891
5	36	-0.43742876424553856
6	1	-0.14727905069270975
6	2	-0.23937053407390607
6	3	-0.009845933583722655
6	4	-0.16325448014138644
6	5	0.15432773519469833
6	6	-0.09413817324778506
6	7	-0.2671213365187981
6	8	-0.08766098828158064
6	9	0.3036082550658925
6	10	-0.010194246813210128
6	11	-0.1437765504266828
6	12	-0.1670355547887432
6	13	-0.0541814992140317
6	14	-0.26784184743566897
6	15	0.07492140047087403
6	16	-0.25797974312291316
6	17	-0.1823281113598192
6	18	-0.09878898178668294
6	19	0.03315056214242418
6	20	0.03597766708015371
6	21	-0.07260225944236964
6	22	-0.10964482199530323
6	23	-0.20206663039634876
6	24	-0.06526293480419554
6	25	-0.19071022095691248
6	26	-0.23610406519640392
6	27	-0.17304822683145105
6	28	-0.12139869824667215
6	29	0.025391964606221507
6	30	-0.29474121489178273
6	31	-0.06478666633938701
6	32	0.13230363790689736
6	33	0.29984389512162224
6	34	0.14528283051832247
6	35	0.08413488357209786
6	36	-0.08179969620573502
7	1	-0.45860772600622113
7	2	-0.46912974829297127
7	3	-0.35821777063421223
7	4	-0.5053127264990138
7	5	-0.43871162589640633
7	6	-0.6593262224116927
7	7	-0.5830815843124739
7	8	-0.632975824860651
7	9	-0.27900239335465926
7	10	-0.43751852426747107
7	11	-0.5153345444577795
7	12	-0.3180631607884621
7	13	-0.46433529182461086
7	14	-0.5171716293742818
7	15	-0.5041546717869565
7	16	-0.7382184764071198
7	17	-0.7776214754807036
7	18	-0.41947782119052235
7	19	-0.6277094966151289
7	20	-0.5680562206197131
7	21	-0.6489956647082487
7	22	-0.5172913452465728
7	23	-0.6386272969961091
7	24	-0.7370625488713038
7	25	-0.5790398411713298
7	26	-0.6815539921074425
7	27	-0.6818209810646607
7	28	-0.9343795102518726
7	29	-0.9573306768529903
7	30	-0.8765729793241022
7	31	-0.4901621591056724
7	32	-0.4907995957496851
7	33	-0.2310425774566518
7	34	-0.4866046592799371
7	35	-0.5133875340149352
7	36	-0.48329872837363297
8	1	-0.417056826971816
8	2	-0.23803669828481944
8	3	-0.3253628225572669
8	4	-0.4704675923167514
8	5	-0.21623261334660526
8	6	-0.4809203455610742
8	7	-0.34869507929745325
8	8	-0.3036999267935556
8	9	0.006667612533362899
8	10	-0.6669458294034882
8	11	-0.3587724682120772
8	12	-0.34953974280129785
8	13	-0.1771791476533835
8	14	-0.3658320270279738
8	15	-0.22973328232617973
8	16	-0.3178163512112917
8	17	-0.03622031122518976
8	18	0.040066383950520645
8	19	-0.03857055968190082
8	20	-0.10100705564943269
8	21	-0.13902231589533542
8	22	-0.21063592876658532
8	23	-0.17259789324166874
8	24	-0.46314708297592944
8	25	-0.15337691658762864
8	26	-0.11055401481358018
8	27	-0.16752846179484493
8	28	-0.325205069000269
8	29	-0.04928537273520206
8	30	-0.3968521054997183
8	31	-0.12172300194321295
8	32	-0.26719386328794315
8	33	0.3549826147429407
8	34	-0.1327155202645507
8	35	-0.1593084852362452
8	36	-0.12881635780976097
9	1	-0.46501686655670227
9	2	-0.4826855328903008
9	3	-0.5776909569198772
9	4	-0.5252613157093862
9	5	-0.530703256601285
9	6	-0.6966772855679579
9	7	-0.5876784342885983
9	8	-0.7343555842484584
9	9	-0.2509138926003267
9	10	-0.9309389356359503
9	11	-0.8785481354861417
9	12	-0.8950590344377893
9	13	-1.3328629570425008
9	14	-1.2514264785639853
9	15	-0.904699104545216
9	16	-1.1578030952257947
9	17	-1.3286559843607435
9	18	-1.3164646842025363
9	19	-1.6327587167684285
9	20	-1.3708954374030995
9	21	-1.5136604167145506
9	22	-1.7105873842176713
9	23	-1.6136551170131455
9	24	-1.5730582051726856
9	25	-1.7328038452559476
9	26	-1.7753487494839675
9	27	-1.8450584075553529
9	28	-1.9360371082972705
9	29	-1.9552410748802131
9	30	-2.119833688473719
9	31	-1.822431952828964
9	32	-1.9104679508938105
9	33	-1.566831865827389
9	34	-2.432260830574382
9	35	-2.4294458773202514
9	36	-1.9420934330424355
10	1	-0.22462229734865957
10	2	-0.0636256904938115
10	3	-0.201459906309438
10	4	-0.24354875551112443
10	5	-0.18147811370345224
10	6	-0.5440437784661256
10	7	-0.30760435577123957
10	8	-0.38487387945145457
10	9	-0.0779514642335819
10	10	-0.3788700648814114
10	11	-0.4453905059127834
10	12	-0.39450191550383096
10	13	-0.5669156082968954
10	14	-0.9335991155293186
10	15	-0.5483304837208642
10	16	-0.6525467581847479
10	17	-0.8819720703786409
10	18	-0.7606581998257269
10	19	-0.7093730292458424
10	20	-0.725314083280697
10	21	-0.976000097911129
10	22	-0.5871373768652219
10	23	-0.6171096773273916
10	24	-0.8092433351268404
10	25	-0.8797224597464073
10	26	-1.152373802797264
10	27	-1.149355916793855
10	28	-1.067496933945094
10	29	-1.2407000215996007
10	30	-1.1381225542590165
10	31	-1.2227027657066856
10	32	-1.02248793831194
10	33	-0.8762236924749089
10	34	-1.329246168474139
10	35	-1.5549928112109228
10	36	-1.1332533823190474
11	1	-0.5198807257989273
11	2	-0.6979009029089234
11	3	-0.14480871621285019
11	4	-0.22505422756377058
11	5	-0.04852473041176353
11	6	0.12497486616696207
11	7	0.11215790941824721
11	8	0.1891036908449344
11	9	0.4814918855990591
11	10	0.18958854816859017
11	11	0.3495647132158304
11	12	0.3751291442128668
11	13	0.26634898889258807
11	14	0.3841699608124358
11	15	0.42649364190798167
11	16	0.3945311965700604
11	17	0.40171603245456566
11	18	0.3840152222372808
11	19	0.4313072930212378
11	20	0.4467226769081094
11	21	0.4741990109368288
11	22	0.499420975544993
11	23	0.3128446865174368
11	24	0.3950119489016437
11	25	0.3948218637872536
11	26	0.40199381076265084
11	27	0.41925953138265015
11	28	0.26872248436372104
11	29	0.32983024858916465
11	30	0.601923367861005
11	31	0.4845943591567625
11	32	0.6821500474056172
11	33	0.9789609027159294
11	34	0.7468531850008802
11	35	0.6490004816778487
11	36	0.7956622944979799
12	1	0.15254962069703382
12	2	0.413530046339158
12	3	0.48180534522534657
12	4	0.6107890617670471
12	5	0.5772613701245319
12	6	0.6123898825490954
12	7	0.6049171397366493
12	8	0.6102885829512346
12	9	0.9873659650827911
12	10	0.7039923648920343
12	11	0.6680451765432416
12	12	0.7503541857188409
12	13	0.6465414296229177
12	14	0.5566580843610716
12	15	0.71332542191616
12	16	0.49933627046681295
12	17	0.5042306325625248
12	18	0.7298459851628003
12	19	0.6580923346067387
12	20	0.357755726858219
12	21	0.5958220630721165
12	22	0.5774408296302376
12	23	0.5597933926716898
12	24	0.5814515796471582
12	25	0.5317108062304783
12	26	0.39284435847869403
12	27	0.43670919651077256
12	28	0.3157999408364063
12	29	0.6111529473663069
12	30	0.37275876644125105
12	31	0.6893436284685066
12	32	0.3098900973293439
12	33	0.7908819716139845
12	34	0.6898907060574331
12	35	0.6649218213446417
12	36	0.8267850246301691
13	1	-14.774517245138899
13	2	-15.990575910207783
13	3	-20.518175353802384
13	4	-21.460814900855436
13	5	-17.506943161600095
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118	33	-0.3602245783204481
118	34	-0.8627932264044524
118	35	-0.832222174174376
118	36	-0.849926071633945
119	1	-1.0229310549127426
119	2	-1.0498530199850542
119	3	-1.2078858704367263
119	4	-1.2191494507209413
119	5	-0.9893748341449715
119	6	-1.0807757750852716
119	7	-1.0780842767380712
119	8	-1.1450018792528809
119	9	-0.9140224387444249
119	10	-1.2849651313359836
119	11	-0.9985617658904715
119	12	-1.049348602491449
119	13	-1.029223358823785
119	14	-1.0195379587754054
119	15	-0.9086304676805987
119	16	-1.2264255532948205
119	17	-1.4014899081706957
119	18	-1.0871999425248156
119	19	-1.122936544283526
119	20	-1.220988600348523
119	21	-1.6655471156311457
119	22	-4.394432808256401
119	23	-1.748032617141078
119	24	-1.7432071025379408
119	25	-1.73100469916956
119	26	-1.4475600610249195
119	27	-1.4938777817111664
119	28	-1.3454977060412503
119	29	-1.3804237101618746
119	30	-1.4340079111405046
119	31	-1.0250282440861298
119	32	-1.3139628232929859
119	33	-0.7017842168271144
119	34	-1.1948964066789962
119	35	-1.1610020254122233
119	36	-0.9641048775384604
120	1	-24.59629141619777
120	2	-23.272987578129364
120	3	-23.021033771493897
120	4	-22.757253730716837
120	5	-22.452117315926763
120	6	-21.935902532757446
120	7	-21.720442893180646
120	8	-21.48871018265019
120	9	-20.908505657465557
120	10	-21.029679285087393
120	11	-20.92432898337995
120	12	-20.73753499272033
120	13	-20.58544320897261
120	14	-20.513867220809686
120	15	-20.263577609218114
120	16	-20.251315217669244
120	17	-20.019702984225297
120	18	-19.782237405318615
120	19	-19.684878451293688
120	20	-19.56867411815618
120	21	-19.35971215719945
120	22	-19.132475505577553
120	23	-18.98553646605825
120	24	-18.79621952171068
120	25	-18.6238508451205
120	26	-18.29378299030748
120	27	-18.036010595013025
120	28	-17.80064499127167
120	29	-17.594141250960398
120	30	-17.470829218745966
120	31	-17.124125031523263
120	32	-16.847756316057023
120	33	-16.27434913523007
120	34	-16.2953788042978
120	35	-16.01243382259995
120	36	-15.923537290552932
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	1.0826928E7
3	540341.0
4	491263.0
5	587616.0
6	529351.0
7	245330.0
8	279180.0
9	347043.0
10	259946.0
11	209846.0
12	229614.0
13	252102.0
14	237955.0
15	227322.0
16	236399.0
17	250617.0
18	265065.0
19	284398.0
20	317534.0
21	357141.0
22	392565.0
23	429155.0
24	480356.0
25	538424.0
26	603607.0
27	674422.0
28	767357.0
29	891736.0
30	1050817.0
31	1253999.0
32	1510155.0
33	1845398.0
34	2288073.0
35	2784385.0
36	3143007.0
37	2938442.0
38	1864536.0
39	666097.0
40	113.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.25087449604284	29.744797432828285	23.671049340779877	27.333278730348997
2	25.310095783850546	29.75904866059418	24.02433207872288	20.9065234768324
3	24.13578935333479	27.59596609238179	24.661139445053205	23.60710510923021
4	26.952617053394885	26.331636147795795	23.24019633943728	23.475550459372037
5	26.872261332725206	27.727978315840783	24.273541095980033	21.126219255453982
6	25.918514547013128	26.573516871360862	21.968270239955334	25.539698341670675
7	24.636946297037955	26.16491959304496	25.320022372348244	23.878111737568837
8	24.691237629065743	25.76228200437938	24.90321348905955	24.643266877495332
9	23.697619368735992	25.530047978826552	25.23176439393974	25.540568258497707
10	24.146858080453395	26.833306343499412	23.521735835351514	25.49809974069567
11	24.468251518246877	26.14297409334418	24.083189901321976	25.305584487086964
12	24.304258722530232	25.717792656174993	24.796194194986125	25.181754426308657
13	24.087177411170043	25.924317328243397	24.914292008764548	25.074213251822012
14	24.01715049269961	26.033187430693182	24.98987677539968	24.959785301207525
15	23.602843251179085	25.71675970700969	25.186868201395317	25.49352884041591
16	23.80778380014857	25.634785531920805	25.056094056029725	25.5013366119009
17	23.784171887254246	25.495991665456035	25.044081805572578	25.67575464171714
18	23.48532698671687	25.573070424768	24.901060612475632	26.040541976039496
19	23.909160966887868	25.30042179957503	24.873321512239023	25.917095721298082
20	23.842085165425157	25.4692874098742	24.941397188615685	25.747230236084956
21	23.732738258945147	25.4501810848663	24.8223292444124	25.99475141177615
22	23.767346376113938	25.240244767352156	24.66413374425066	26.32827511228325
23	23.815170033604574	25.384721226158774	24.845106348825144	25.955002391411508
24	23.84628373242526	25.287366275610147	24.595227184870847	26.271122807093743
25	23.98686994318318	25.25060393695138	24.460215721277933	26.302310398587515
26	24.045815433976436	25.246824117154283	24.577287793020126	26.13007265584916
27	24.06747444914819	25.03158756162418	24.434814200161252	26.466123789066376
28	24.0439971431543	25.077401469605697	24.272904992741314	26.60569639449869
29	24.035260453792358	25.039918577907837	24.368263034360364	26.55655793393944
30	23.865259789874486	25.081029177138376	24.557083097542055	26.496627935445087
31	23.85358394478463	24.964272338922235	24.458857840880764	26.723285875412365
32	23.832145831415644	25.10330752276037	24.47318239427408	26.591364251549905
33	23.65401769942588	25.069703430348778	24.149021749466453	27.127257120758898
34	23.872388447752225	24.832632013768052	24.223755058593518	27.0712244798862
35	23.822811849426355	24.933438846447977	24.36916891991898	26.874580384206688
36	23.838759125785856	24.808913385056222	24.279095133315604	27.07323235584232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	910272.0
1	1084275.0
2	1258278.0
3	1258278.0
4	1039796.0
5	821314.0
6	821314.0
7	670667.0
8	520020.0
9	520020.0
10	420564.0
11	321108.0
12	321108.0
13	255889.5
14	190671.0
15	169761.0
16	148851.0
17	148851.0
18	168069.0
19	187287.0
20	187287.0
21	239358.5
22	291430.0
23	291430.0
24	366302.5
25	441175.0
26	553735.5
27	666296.0
28	666296.0
29	829616.5
30	992937.0
31	992937.0
32	1177172.0
33	1361407.0
34	1361407.0
35	1567172.5
36	1772938.0
37	1772938.0
38	1981807.0
39	2190676.0
40	2411059.0
41	2631442.0
42	2631442.0
43	2843076.0
44	3054710.0
45	3054710.0
46	3209280.5
47	3363851.0
48	3363851.0
49	3435211.5
50	3506572.0
51	3487155.5
52	3467739.0
53	3467739.0
54	3339769.0
55	3211799.0
56	3211799.0
57	3014404.0
58	2817009.0
59	2817009.0
60	2544463.5
61	2271918.0
62	2271918.0
63	1984168.5
64	1696419.0
65	1438483.0
66	1180547.0
67	1180547.0
68	972317.5
69	764088.0
70	764088.0
71	615080.0
72	466072.0
73	466072.0
74	369144.0
75	272216.0
76	212447.5
77	152679.0
78	152679.0
79	117869.5
80	83060.0
81	83060.0
82	62986.0
83	42912.0
84	42912.0
85	31823.0
86	20734.0
87	20734.0
88	14931.5
89	9129.0
90	6457.0
91	3785.0
92	3785.0
93	2704.0
94	1623.0
95	1623.0
96	1472.5
97	1322.0
98	1322.0
99	2335.5
100	3349.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.2798352021959414
2	2.3503104254052576
3	4.25159257947568
4	5.632440893496669
5	7.578333887095937
6	7.495608932241478
7	8.684599490943944
8	9.078663528935424
9	10.35219666533123
10	9.621040237473519
11	9.780789089201848
12	10.610593529287025
13	10.670173600013722
14	9.91263609207683
15	9.985117148468518
16	10.016255193273286
17	10.057525207959046
18	10.116998216564044
19	10.212105879085257
20	10.242616150540048
21	10.306950752762294
22	10.315162904142781
23	10.3237862713998
24	10.367202394979662
25	10.476795075921036
26	10.44927281095372
27	10.574396799231877
28	10.559274274541588
29	10.557933564790286
30	10.513400588622678
31	10.625893679770137
32	10.600028444459152
33	10.77344718254469
34	10.553332326787174
35	10.55128354709462
36	10.686816893478177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	4.1097635E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.24823149094206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.09598106496263	70.98403915117629
2	3.9403027078619353	6.008822260268854
3	1.2572159380415693	2.8758147563368635
4	0.5615321862200451	1.7126334449808311
5	0.31201155494591765	1.1895164634682558
6	0.1889391613574996	0.8643766147734652
7	0.12229266224618733	0.6527219454413616
8	0.08775895457857874	0.5353172067288433
9	0.05852063311143861	0.4015885302429714
>10	0.3278466221890413	4.45151285586793
>50	0.027150270137950634	1.4657783944249405
>100	0.01945058074726615	2.46573990178202
>500	3.323211600142572E-4	0.17293870000954648
>1k	4.255934334728413E-4	0.6563805462624647
>5k	1.1466257109335904E-4	0.6400882098929657
>10k+	1.250864352613944E-4	4.922731018342373
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	302925	0.7370862094619313	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	227290	0.5530488554876698	No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	153672	0.37391932650139115	No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	109335	0.2660372062771982	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	104629	0.254586425715251	No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	79304	0.1929648749861154	No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	79244	0.19281888118379562	No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	74886	0.1822148646753031	No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	68585	0.16688308220168874	No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	54860	0.13348699992104168	No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	53428	0.13000261450567654	No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	48288	0.11749581210694972	No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	47466	0.11549569701516887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.59642908892446E-5	3.6498450579942134E-5	0.0	2.4332300386628085E-6	2.4332300386628085E-6
2	6.326398100523303E-5	6.326398100523303E-5	2.4332300386628085E-6	2.4332300386628085E-6	2.4332300386628085E-6
3	7.056367112122146E-5	9.002951143052392E-5	2.4332300386628085E-6	4.866460077325617E-6	7.299690115988426E-6
4	7.299690115988427E-5	1.2409473197180323E-4	2.4332300386628085E-6	1.2166150193314044E-5	1.2166150193314044E-5
5	7.786336123720987E-5	1.5815995251308257E-4	2.4332300386628085E-6	1.7032610270639662E-5	1.2166150193314044E-5
6	8.272982131453549E-5	1.8979194301569908E-4	7.299690115988426E-6	2.9198760463953704E-5	1.4599380231976852E-5
7	9.489597150784954E-5	2.1169101336366436E-4	7.299690115988426E-6	2.9198760463953704E-5	1.946584030930247E-5
8	1.0462889166250077E-4	2.2872362363430401E-4	7.299690115988426E-6	3.1631990502616514E-5	2.4332300386628088E-5
9	1.435605722811057E-4	2.3845654378895525E-4	7.299690115988426E-6	3.893168061860494E-5	3.1631990502616514E-5
10	6.764379507482608E-4	2.6765530425290894E-4	7.299690115988426E-6	5.353106085058179E-5	3.6498450579942134E-5
11	7.76200382333436E-4	2.9198760463953707E-4	7.299690115988426E-6	5.353106085058179E-5	4.1364910657267746E-5
12	8.418975933773318E-4	3.1631990502616514E-4	7.299690115988426E-6	6.326398100523303E-5	4.6231370734593366E-5
13	9.002951143052392E-4	3.4795189552878165E-4	7.299690115988426E-6	6.813044108255865E-5	5.1097830811918986E-5
14	9.708587854264606E-4	3.649845057994213E-4	7.299690115988426E-6	7.543013119854706E-5	5.59642908892446E-5
15	0.0027252176433023457	3.722841959154097E-4	7.299690115988426E-6	7.786336123720987E-5	6.083075096657022E-5
16	0.002837146225080835	3.9904972634070065E-4	7.299690115988426E-6	8.759628139186111E-5	7.056367112122146E-5
17	0.003065869848715139	4.209487966886659E-4	7.299690115988426E-6	9.246274146918673E-5	8.029659127587268E-5
18	0.0033992223640119438	4.6718016742325927E-4	7.299690115988426E-6	9.732920154651235E-5	8.029659127587268E-5
19	0.0037715065599273536	4.988121579258758E-4	7.299690115988426E-6	9.976243158517516E-5	8.51630513531983E-5
20	0.04350858632132968	5.231444583125039E-4	7.299690115988426E-6	1.0219566162383797E-4	9.489597150784954E-5
21	0.04445511280636952	5.620761389311088E-4	7.299690115988426E-6	1.119285817784892E-4	9.732920154651235E-5
22	0.044732501030777076	5.693758290470973E-4	9.732920154651234E-6	1.1922827189447763E-4	1.1436181181715201E-4
23	0.04500258956506865	6.034410495883765E-4	1.2166150193314044E-5	1.2896119204912885E-4	1.2409473197180323E-4
24	0.045301876859824175	6.204736598590162E-4	1.2166150193314044E-5	1.4112734224244291E-4	1.3382765212645447E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	5660	0.0	10.949205	20
CGGTTCA	7370	0.0	9.63068	1
CGAGATC	6505	0.0	9.520083	18
GAGATCG	6610	0.0	9.468161	19
GATCGGA	6650	0.0	9.103375	21
AATGCCG	7635	0.0	9.0020075	13
CCGAGAT	7760	0.0	8.227213	17
ATGCCGA	8530	0.0	7.9196405	14
ATCGGAA	8120	0.0	7.5680246	22
TGCCGAG	8890	0.0	7.452434	15
AGCGGTT	7250	0.0	7.3714447	30
TCGGAAG	8980	0.0	7.3603463	23
GCGGTTC	6600	0.0	7.3471437	11
GAGCGGT	7370	0.0	7.2270336	29
GCCGAGA	8810	0.0	7.177629	16
AGTGGTA	13690	0.0	6.6790137	2
TCAACGC	14015	0.0	6.619846	9
AAGAGCG	8885	0.0	6.5864263	27
CAACGCA	14425	0.0	6.4791374	10
AAGCAGT	12180	0.0	6.3426986	1
>>END_MODULE