FastQCFastQC Report
Fri 27 May 2016
SRR522050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37332264
Sequences flagged as poor quality0
Sequence length36
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3925721.0515622626048076No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3229350.8650292411946943No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2193800.5876418317410377No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1829930.49017386140845887No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1724540.4619435885270714No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN808740.21663299070209083No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN796030.2132284288999992No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN756410.20261562491897092No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN661220.1771175731533453No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN508040.1360860407501672No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN499570.1338172257648237No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN497640.1333002466713511No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN485780.13012336996223964No Hit
CCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN433620.11615154119771573No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN421830.11299341502567324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39150.010.51115721
CGGTTCA45150.010.4394221
CGAGATC43600.09.74600518
AGATCGG43200.09.63087820
GAGATCG45300.09.1475619
AATGCCG50700.09.06426713
AGCGGTT47250.07.83004230
ATGCCGA62500.07.306784614
CCGAGAT61700.06.983106617
TGCCGAG65450.06.962456715
ATCGGAA62500.06.92649522
TCGGAAG64050.06.82586423
AGTGGTA136650.06.76418972
GCCGAGA67800.06.75516516
GAGCGGT57500.06.72015829
AAGAGCG61500.06.576318327
AAGCAGT112800.06.51747851
GGTATCA149850.06.47786435
TCAACGC146650.06.44075639
GTATCAA147150.06.42261656
Produced by FastQC (version 0.11.5)