##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522050_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37332264
Sequences flagged as poor quality	0
Sequence length	36
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.53812241336341	35.0	19.0	39.0	2.0	40.0
2	26.457434191507915	34.0	2.0	39.0	2.0	40.0
3	26.184084308414835	35.0	2.0	39.0	2.0	40.0
4	25.880064868286585	34.0	2.0	39.0	2.0	39.0
5	25.588951931766047	34.0	2.0	39.0	2.0	39.0
6	25.004238130320733	34.0	2.0	39.0	2.0	39.0
7	24.736341439136936	33.0	2.0	39.0	2.0	39.0
8	24.518478868573307	33.0	2.0	39.0	2.0	39.0
9	24.338801847110048	33.0	2.0	39.0	2.0	39.0
10	24.15673043563605	33.0	2.0	38.0	2.0	39.0
11	23.958255947188203	33.0	2.0	39.0	2.0	39.0
12	23.79702514157727	33.0	2.0	38.0	2.0	39.0
13	23.649228292181796	33.0	2.0	38.0	2.0	39.0
14	23.53056305398462	33.0	2.0	38.0	2.0	39.0
15	23.41115079439061	33.0	2.0	38.0	2.0	39.0
16	23.20877300664112	33.0	2.0	38.0	2.0	39.0
17	23.04578449889886	32.0	2.0	38.0	2.0	39.0
18	22.887056488189412	32.0	2.0	38.0	2.0	39.0
19	22.77182471440789	31.0	2.0	38.0	2.0	39.0
20	22.614515583624932	31.0	2.0	38.0	2.0	39.0
21	22.403993071515835	31.0	2.0	38.0	2.0	39.0
22	21.93663132779732	31.0	2.0	38.0	2.0	39.0
23	22.08131673985805	31.0	2.0	38.0	2.0	39.0
24	21.93436628970587	30.0	2.0	38.0	2.0	39.0
25	21.775145782747064	30.0	2.0	38.0	2.0	39.0
26	21.533072277641665	30.0	2.0	38.0	2.0	39.0
27	21.31564996968842	29.0	2.0	37.0	2.0	39.0
28	21.128316032480644	29.0	2.0	37.0	2.0	39.0
29	21.085534164228562	29.0	2.0	37.0	2.0	39.0
30	20.912845976874053	29.0	2.0	36.0	2.0	39.0
31	20.84435854198395	29.0	2.0	37.0	2.0	39.0
32	20.586663857300483	28.0	2.0	36.0	2.0	39.0
33	20.35910455363757	27.0	2.0	36.0	2.0	39.0
34	20.149560685631066	27.0	2.0	36.0	2.0	39.0
35	19.892460794769907	27.0	2.0	36.0	2.0	39.0
36	19.878215877826214	27.0	2.0	36.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-12.003630874613503
1	2	-12.960025588669978
1	3	-13.81020105014337
1	4	-15.185345808693137
1	5	-19.81534774196503
1	6	-20.86481478136921
1	7	-20.923838797913888
1	8	-21.03143834642248
1	9	-21.136722620737142
1	10	-21.08413399871515
1	11	-21.17101450734246
1	12	-21.08064635344945
1	13	-20.973944275204165
1	14	-20.947035233336948
1	15	-20.871801802181803
1	16	-20.751666592820236
1	17	-20.608447443999665
1	18	-20.51440105813971
1	19	-20.436982938541245
1	20	-20.334149211091862
1	21	-20.17347883372485
1	22	-19.772728985632813
1	23	-19.901696058897308
1	24	-19.769832257288257
1	25	-19.638742024086415
1	26	-19.45091586359891
1	27	-19.241128211500524
1	28	-19.062976786325663
1	29	-19.033237428066492
1	30	-18.85332610113661
1	31	-18.798631985140563
1	32	-18.54293779312284
1	33	-18.317658187966156
1	34	-18.106158566918793
1	35	-17.85082746889222
1	36	-17.840823086236036
2	1	-0.7857429396626898
2	2	-0.6731493380617444
2	3	-0.6116648049994566
2	4	-0.5721795181714846
2	5	-0.49505663551250123
2	6	-0.6547031176976894
2	7	-0.5543126670927983
2	8	-0.5695953099226365
2	9	-0.5326906829692248
2	10	-0.511678493044041
2	11	-0.5679911775054585
2	12	-0.6381647640919468
2	13	-0.6307701228600244
2	14	-0.56206256373477
2	15	-0.61842639027277
2	16	-0.6440407529020327
2	17	-0.6057289736088087
2	18	-0.7488381001184763
2	19	-0.7951551119741254
2	20	-0.8089369277629288
2	21	-2.6657079180556735
2	22	-19.959221481463832
2	23	-2.612952997143502
2	24	-2.495765949292931
2	25	-2.4256602456252523
2	26	-2.7496254280098213
2	27	-2.6625758419734424
2	28	-2.6042030313304885
2	29	-2.8835383254269438
2	30	-2.6375502962483566
2	31	-0.7268555868187399
2	32	-0.6361731939295048
2	33	-0.5313060304359745
2	34	-0.4891287072874704
2	35	-0.4107457524069211
2	36	-0.5816214632890002
3	1	-0.14795156923904074
3	2	0.03663747162823228
3	3	0.10477896338449
3	4	0.13162702683220218
3	5	0.2908822707779528
3	6	0.15940895964765645
3	7	0.24011576629598252
3	8	0.2552432648786578
3	9	0.2955832575599544
3	10	0.25124508695645886
3	11	0.22444302139175676
3	12	0.2735198083984116
3	13	0.2913553164541476
3	14	0.3059945196630238
3	15	0.244521532756675
3	16	0.34173887458931773
3	17	0.3756650876822647
3	18	0.35813755240029366
3	19	0.18936802614715376
3	20	0.17638393671645858
3	21	-1.0065203675189274
3	22	-14.944491814181022
3	23	-1.0575130171216287
3	24	-1.0234438951233855
3	25	-1.0275292214191794
3	26	-1.1956811030903758
3	27	-1.0996228503176901
3	28	-1.166350666277225
3	29	-1.3473074805482383
3	30	-1.2134604859714777
3	31	0.4514896101901762
3	32	0.31096509055629085
3	33	0.37403797296211394
3	34	0.37743405669310093
3	35	0.318154810547842
3	36	0.3548192486352022
4	1	0.24847576120655646
4	2	0.46094658454870796
4	3	0.5020953711722633
4	4	0.5264675844379809
4	5	0.624334540897447
4	6	0.5819741029447556
4	7	0.6327489351059441
4	8	0.5583869260466123
4	9	0.6511788151463556
4	10	0.7642808663021121
4	11	0.5966140562153335
4	12	0.7612290292470654
4	13	0.7569446044694139
4	14	0.7779640698497872
4	15	0.7911091605930594
4	16	0.9569836080042435
4	17	0.9465703504835048
4	18	0.9356438387914707
4	19	0.9757586677670425
4	20	0.960070476769534
4	21	1.0865435629990507
4	22	1.2366902301429477
4	23	0.9489255954705733
4	24	0.998723111972037
4	25	1.1245187981159717
4	26	1.0619030959056772
4	27	1.1797058076305085
4	28	1.0603607727048718
4	29	1.1276555836405002
4	30	0.9897983460865305
4	31	1.1262992456220076
4	32	1.1790322040997268
4	33	1.176916173440052
4	34	1.1780478702861927
4	35	1.0517706764690935
4	36	1.2007793219103178
5	1	0.5297964927539844
5	2	0.7295286607124609
5	3	0.7799962366853386
5	4	0.8194214504992416
5	5	0.8514729519931201
5	6	0.8292403175906351
5	7	0.7879858435069202
5	8	0.7618500162002171
5	9	0.87750987234201
5	10	0.6213665165803413
5	11	0.8611806335614922
5	12	0.8779336979939565
5	13	0.8487572176063871
5	14	0.8625307632658696
5	15	0.8937985223648823
5	16	1.0068384797911172
5	17	1.0618786888471448
5	18	1.0912569095046436
5	19	0.9158160399784911
5	20	1.1056771929270042
5	21	0.897882450884822
5	22	1.3586052542510814
5	23	0.9757843666531123
5	24	1.1136058978173224
5	25	1.1863221484402402
5	26	1.1322765547530587
5	27	0.877411555239842
5	28	1.1562173040701467
5	29	1.0916567200148073
5	30	1.1497342103995223
5	31	1.1229298585883143
5	32	1.2314770803042947
5	33	1.2326780203390904
5	34	1.2752288554107238
5	35	1.1363751820653256
5	36	1.1325078600740852
6	1	0.6180275371889188
6	2	0.7269973758410515
6	3	0.8097161855426478
6	4	0.7836771158835596
6	5	0.8620997498151652
6	6	0.8284421350214046
6	7	0.9143685648833113
6	8	0.973196346426743
6	9	0.9080579137341118
6	10	0.8578063050727991
6	11	0.9887248912552984
6	12	1.006966096351615
6	13	0.9563381664906885
6	14	0.9197214635372823
6	15	1.0217487997205161
6	16	0.9879768435418832
6	17	1.0174369296483583
6	18	0.9957125974872021
6	19	0.936770731895006
6	20	0.9785083823832537
6	21	1.0477303445824866
6	22	1.3902072494081992
6	23	0.9967198215274777
6	24	0.9535655298304953
6	25	1.003101495269238
6	26	1.069048372479088
6	27	1.184486460092728
6	28	1.12092387984465
6	29	1.131115520714232
6	30	1.148996922787763
6	31	1.1651877700867885
6	32	1.0417139895312708
6	33	1.093138592921683
6	34	1.1759179965368851
6	35	1.2373005191114146
6	36	1.2018743731648627
7	1	0.6264653403595517
7	2	0.880773052958638
7	3	0.9000141643509316
7	4	0.8916936298963556
7	5	1.0286234798005438
7	6	1.0389134984752317
7	7	0.9547088407972844
7	8	0.921079785326242
7	9	1.012247413684964
7	10	1.0831208768261114
7	11	1.0577277496165003
7	12	1.1244416033879148
7	13	1.1570324814167918
7	14	1.2028828476036253
7	15	1.1172807488404821
7	16	1.238898756075816
7	17	1.1565138426411785
7	18	1.2444955378800238
7	19	1.164019058751137
7	20	1.2105788295679005
7	21	1.2036161947700315
7	22	1.5648526473453188
7	23	1.3200871639733691
7	24	1.304466384248169
7	25	1.3121748666117696
7	26	1.1746243627092667
7	27	1.315322907880141
7	28	1.3030656419728288
7	29	1.2269937028343882
7	30	1.229806329059926
7	31	1.1708011920776826
7	32	1.2012759481758053
7	33	1.0869468455789644
7	34	0.8371632011636052
7	35	1.1662028187104738
7	36	0.9747816527929949
8	1	0.3371902632244961
8	2	0.5969850032384727
8	3	0.6050570011798548
8	4	0.6463054311536283
8	5	0.7690457851976298
8	6	0.6324471379095691
8	7	0.7287593335266571
8	8	0.7585653461428201
8	9	0.7184615469470259
8	10	0.795021160028206
8	11	0.8150727828952853
8	12	0.8012957484991361
8	13	0.8600386883183582
8	14	0.8399833441728752
8	15	0.8483595020829391
8	16	0.9853264019953905
8	17	0.9309780150107514
8	18	1.0365434097956765
8	19	0.9883850444629267
8	20	1.0190791375158632
8	21	1.1217735177450763
8	22	1.4045308927052105
8	23	1.0772160840469347
8	24	1.1430955055503347
8	25	1.2186592169480939
8	26	1.2712048889612042
8	27	1.1495727134092206
8	28	1.0939565626067136
8	29	1.1376610068559394
8	30	0.9345822288842385
8	31	1.0475247478919805
8	32	0.9098175791769556
8	33	1.0441529994773262
8	34	0.8695048642002128
8	35	0.9393609674132719
8	36	0.6580397475003394
9	1	0.7058699404561644
9	2	0.8378235899876181
9	3	0.9092597539587217
9	4	0.7645586546104148
9	5	1.0112708583438454
9	6	0.9521974542064662
9	7	0.8573657940521109
9	8	0.7120827622626251
9	9	0.6669471464894734
9	10	0.8194646655125979
9	11	0.5923084302544943
9	12	0.9123353604932127
9	13	0.7646970136520963
9	14	0.9270286121193294
9	15	0.7768625657597141
9	16	0.8598993655200076
9	17	0.9593374620846227
9	18	0.8894329912011543
9	19	1.015174911814018
9	20	0.897774592299406
9	21	0.9545963401078446
9	22	1.3154557363620754
9	23	1.1245634713958843
9	24	0.9764265522256466
9	25	1.094212671179701
9	26	1.1265212855147588
9	27	1.049323217300362
9	28	1.0086493948989848
9	29	0.808325638153292
9	30	0.967236551213869
9	31	0.8863697940421424
9	32	0.8035689006710598
9	33	1.0066631405849265
9	34	0.9002101946200725
9	35	1.0812540287626362
9	36	1.1965445223389324
10	1	0.6907290945913189
10	2	0.8615735474085042
10	3	0.9541278633790284
10	4	0.9631963924185989
10	5	0.8147649814183744
10	6	1.077985225669
10	7	1.1869064292755098
10	8	1.2708740657673054
10	9	1.2069890037185012
10	10	1.1732262438238017
10	11	1.416947010045103
10	12	1.4131933381885524
10	13	1.434938825850054
10	14	1.4148891670126957
10	15	1.5853614799271156
10	16	1.4802358076540436
10	17	1.457048512788866
10	18	1.426928779076487
10	19	1.5522164990386251
10	20	1.5666094663120909
10	21	1.6203129708808568
10	22	1.9759833789198495
10	23	1.5623045617945195
10	24	1.6283046049125467
10	25	1.7476462653596556
10	26	1.6475080786469718
10	27	1.6812050643696281
10	28	1.682031386960272
10	29	1.6121218087161289
10	30	1.674770178438365
10	31	1.5130702449730435
10	32	1.5391390892649959
10	33	1.660284552970463
10	34	1.6269180006147224
10	35	1.5368178948936304
10	36	1.551254510157758
11	1	0.5076438193663044
11	2	0.7106281750450165
11	3	0.7477531217197537
11	4	0.7465299766841049
11	5	0.926460024438331
11	6	0.855101962085282
11	7	0.9179371832911336
11	8	0.8236567574986005
11	9	0.7887983586105811
11	10	0.7325598689959847
11	11	0.3336173451039919
11	12	0.696251890126149
11	13	0.3558308067275213
11	14	0.5447324574322892
11	15	0.5061750473476714
11	16	0.7138313899183082
11	17	0.6093863923958587
11	18	0.5878068339915359
11	19	0.5815327056824948
11	20	0.7936069237656831
11	21	0.7777996361432855
11	22	0.6422743733961944
11	23	0.760856543943703
11	24	0.5706618880070309
11	25	0.9118093106711456
11	26	0.8307154733964452
11	27	1.0780343334534024
11	28	1.1086190924342993
11	29	1.098621836176033
11	30	1.057740625495466
11	31	1.2055333744578682
11	32	1.1223277976883672
11	33	1.2408594582212125
11	34	1.1156791837467637
11	35	1.1962723616161561
11	36	1.2250649313779185
12	1	0.8672085822616928
12	2	1.03236085970223
12	3	1.1096395674475978
12	4	1.0796805926958015
12	5	1.2463009551825905
12	6	1.2731998362482564
12	7	1.2676934334457641
12	8	1.2167858652123869
12	9	1.2071641605650534
12	10	1.2585368646876596
12	11	1.2348461758825167
12	12	1.2270309933287962
12	13	1.2831087818482345
12	14	1.1949343921716853
12	15	1.3231225505376933
12	16	1.3125296697433662
12	17	1.378880602207392
12	18	1.4728965153567266
12	19	1.4347123500444638
12	20	1.4514063928879146
12	21	1.4373632656974458
12	22	1.7323968157302367
12	23	1.3938088400559856
12	24	1.517380035525985
12	25	1.5098318080129793
12	26	1.5332730689826661
12	27	1.4096235682228908
12	28	1.3870571422992377
12	29	1.3895081208657167
12	30	1.4783406281917806
12	31	1.5567242271941772
12	32	1.4734598352003303
12	33	1.386989586830996
12	34	1.3560977466612023
12	35	1.4747250412125936
12	36	1.365223055441895
13	1	0.9260062599916914
13	2	1.1209752006596219
13	3	1.2015581390760097
13	4	1.2051742951445554
13	5	1.2236981794786423
13	6	1.216358473930054
13	7	1.1856339851746682
13	8	1.156702911490985
13	9	1.171874237966744
13	10	1.233894948474763
13	11	1.342579917284347
13	12	1.3092280992905323
13	13	1.4007148807790983
13	14	1.441732475402656
13	15	1.3662843146066876
13	16	1.4439694558048046
13	17	1.4699898447558937
13	18	1.3132839658080435
13	19	1.4559705562067613
13	20	1.287365265691875
13	21	1.3319149467922706
13	22	1.6461027670814978
13	23	1.3273460490264206
13	24	1.4525086536453706
13	25	1.4028864663689333
13	26	1.4092444562573192
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118	15	1.1069557424709728
118	16	1.2083410100354115
118	17	1.3144816889216244
118	18	1.2880264692439205
118	19	1.2786887083692555
118	20	1.3350974598501395
118	21	1.3557732649561594
118	22	1.6291646152392012
118	23	1.3343236384906092
118	24	1.4434062802861511
118	25	1.332172104087121
118	26	1.3535100756734018
118	27	1.335766994748976
118	28	1.294278046794009
118	29	1.1566622681252774
118	30	1.198624599032776
118	31	1.0236349479353635
118	32	1.323767386033147
118	33	1.2240611788824651
118	34	1.334932513787301
118	35	1.2502718697452835
118	36	1.1350676237524162
119	1	0.5910249187769345
119	2	0.42846569728157746
119	3	0.43398410877137295
119	4	0.5324843889713158
119	5	0.6226497297728031
119	6	0.6068138173107265
119	7	0.6550025209146391
119	8	0.7406501916965205
119	9	0.6424264973028464
119	10	0.8534332541333569
119	11	0.7800299123792804
119	12	0.7779476079374916
119	13	0.9107730042393101
119	14	0.9078781860958891
119	15	0.9778803720787259
119	16	0.913248399535199
119	17	0.9433236093523014
119	18	0.9910405635022919
119	19	1.04491168934549
119	20	1.0686252681960866
119	21	1.1167474171263692
119	22	1.3984223606664585
119	23	1.1687025797327877
119	24	1.1831654080637684
119	25	1.1737629098816704
119	26	1.2515128755845737
119	27	1.2363767872258968
119	28	1.1999431009666495
119	29	1.2588680093337423
119	30	1.238706721028823
119	31	1.1195085117593635
119	32	1.2578666540300034
119	33	1.0903852846539763
119	34	1.2089329282330077
119	35	1.2604196181943443
119	36	1.17351646777378
120	1	-25.538403279035713
120	2	-24.45848152097805
120	3	-24.1811787538059
120	4	-23.8776439990458
120	5	-23.58648602866752
120	6	-23.002761788966023
120	7	-22.73438169211437
120	8	-22.516244720333912
120	9	-22.335135319149842
120	10	-22.151856807683096
120	11	-21.95320831871915
120	12	-21.789991051120996
120	13	-21.642216772270437
120	14	-21.525134177194648
120	15	-21.40410369829693
120	16	-21.19632720674155
120	17	-21.03578361841755
120	18	-20.878461338542404
120	19	-20.761793018215137
120	20	-20.608591802655077
120	21	-20.39659661520839
120	22	-19.959221481463832
120	23	-20.075650725069874
120	24	-19.92507282303466
120	25	-19.767362862823987
120	26	-19.528860400687417
120	27	-19.304249698746524
120	28	-19.119273494373342
120	29	-19.078447730086133
120	30	-18.90252009780142
120	31	-18.829760389809824
120	32	-18.57258389280785
120	33	-18.34384557602125
120	34	-18.13206802278934
120	35	-17.872753230117592
120	36	-17.85780783084724
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	9531055.0
3	546311.0
4	550701.0
5	611681.0
6	540088.0
7	234681.0
8	251487.0
9	302530.0
10	235253.0
11	171346.0
12	180390.0
13	197213.0
14	189101.0
15	164355.0
16	167413.0
17	174490.0
18	177339.0
19	178499.0
20	197289.0
21	229510.0
22	264360.0
23	295697.0
24	330982.0
25	368373.0
26	411335.0
27	458065.0
28	514346.0
29	590779.0
30	695078.0
31	836787.0
32	1040308.0
33	1326572.0
34	1743193.0
35	2298446.0
36	2974422.0
37	3538183.0
38	3053151.0
39	1761136.0
40	319.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.976059738910013	30.10901334737995	21.727091514821346	29.187835398888694
2	24.873973376285207	30.009726420793864	23.229366231979025	21.886933970941904
3	23.472891618589156	27.840960506405978	23.808768093571384	24.877379781433483
4	26.566000897521164	26.616541232011524	22.11580813340504	24.701649737062272
5	26.351493575369144	28.11239904319266	23.164021402045243	22.37208597939295
6	25.993209390659576	26.70752274904162	20.706125660559884	26.593142199738917
7	24.685408829313417	26.15323897194312	24.239731528500762	24.9216206702427
8	24.689858229115437	25.580846986224042	24.047539174121653	25.681755610538872
9	24.0619454146725	25.300089887743788	24.343445174493095	26.29451952309062
10	24.362330178848836	26.860034309486124	22.403371900426706	26.374263611238334
11	24.7473420292767	26.00549192868345	23.164017347450354	26.083148694589497
12	24.603703005264713	25.566854929095793	23.858466713729275	25.97097535191022
13	24.313554783185438	25.813337477130005	23.86082657631284	26.012281163371714
14	24.284422343689446	25.86165989866764	24.090907875486042	25.76300988215687
15	23.908931198479678	25.559025495126207	24.263787178706487	26.26825612768763
16	24.082104015457347	25.447412337683556	24.155144965943183	26.31533868091591
17	24.14426564753503	25.346821856838748	24.159942386252727	26.3489701093735
18	23.91940084495204	25.20412076184501	24.200140951976408	26.676337441226543
19	24.201820071596085	25.25077959374242	24.11620667434428	26.43119366031722
20	24.16648368550201	25.261321406694986	24.2011124872395	26.371082420563507
21	24.064280324699403	25.183342967468015	24.078478421024208	26.673898286808377
22	24.04673405501202	25.138547641739567	23.88296599076882	26.93175231247959
23	24.102865822852753	25.18723277851772	24.11837253778013	26.5915288608494
24	24.167514888930643	24.998527853732867	23.92465609816791	26.909301159168574
25	24.28363228814798	24.996935659986274	23.763308260874112	26.956123790991633
26	24.38282086700902	25.044013516000906	23.85697274793439	26.71619286905569
27	24.373499792350795	24.805271576026158	23.797812148551035	27.023416483072015
28	24.436741909345287	24.816701546727398	23.64088410477676	27.105672439150556
29	24.340285514764872	25.016737622521713	23.958401033284265	26.684575829429146
30	24.194300491690367	24.891776838948665	23.88633029533983	27.027592374021136
31	24.226691650663128	24.88281371931067	23.861338395770566	27.029156234255638
32	24.244225043210616	24.882156518968372	23.94586925005032	26.927749187770694
33	24.207518616826693	24.722088200019307	23.80462562611677	27.26576755703723
34	24.249947108245657	24.740440014001976	23.639595479989154	27.370017397763213
35	24.189337984791912	24.8137236197796	23.8140688452209	27.182869550207588
36	24.326304328883033	24.57008187129756	23.825156072357228	27.27845772746218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1225798.0
1	1237325.5
2	1248853.0
3	1248853.0
4	987858.5
5	726864.0
6	726864.0
7	569757.0
8	412650.0
9	412650.0
10	327097.0
11	241544.0
12	241544.0
13	197006.0
14	152468.0
15	139382.5
16	126297.0
17	126297.0
18	139470.0
19	152643.0
20	152643.0
21	191512.0
22	230381.0
23	230381.0
24	287118.0
25	343855.0
26	434186.5
27	524518.0
28	524518.0
29	662228.0
30	799938.0
31	799938.0
32	957090.5
33	1114243.0
34	1114243.0
35	1294411.0
36	1474579.0
37	1474579.0
38	1653385.5
39	1832192.0
40	2027953.5
41	2223715.0
42	2223715.0
43	2420488.5
44	2617262.0
45	2617262.0
46	2760738.5
47	2904215.0
48	2904215.0
49	2993567.0
50	3082919.0
51	3098025.0
52	3113131.0
53	3113131.0
54	3030856.0
55	2948581.0
56	2948581.0
57	2797204.5
58	2645828.0
59	2645828.0
60	2427487.0
61	2209146.0
62	2209146.0
63	1955928.5
64	1702711.0
65	1463819.0
66	1224927.0
67	1224927.0
68	1024846.0
69	824765.0
70	824765.0
71	672837.0
72	520909.0
73	520909.0
74	418073.0
75	315237.0
76	248201.0
77	181165.0
78	181165.0
79	142320.5
80	103476.0
81	103476.0
82	79472.5
83	55469.0
84	55469.0
85	41425.0
86	27381.0
87	27381.0
88	20000.5
89	12620.0
90	9007.0
91	5394.0
92	5394.0
93	3814.5
94	2235.0
95	2235.0
96	1769.5
97	1304.0
98	1304.0
99	2177.5
100	3051.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.9562810334781733
2	3.610292694812187
3	5.631450586548943
4	7.07473835500574
5	8.184215669320242
6	8.985415403684064
7	9.514539487881047
8	10.051303076609551
9	10.293281436132563
10	10.506359860736012
11	10.624075732454909
12	10.739439751095729
13	10.81077483005049
14	10.842982895438649
15	10.958057620078975
16	11.056396151061183
17	11.097540186686777
18	11.160207160219375
19	11.239862120336447
20	11.275895295286672
21	11.366363957996226
22	12.369206967999585
23	11.366503247700166
24	11.387769035384514
25	11.406374925453221
26	11.447655572134602
27	11.461729189528928
28	11.468324021280896
29	11.511235964687275
30	11.564744640185767
31	11.525116719414607
32	11.562783869737983
33	11.509328767202547
34	11.487010270794185
35	11.545948566098215
36	11.560207010215079
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3.7332264E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.39126817581187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.99308761008349	92.38488173110674
2	0.005024438321540644	0.009284284567965755
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	1.1271905027699883E-4	9.372830402398519E-4
>10	8.020929354619187E-4	0.014312636033752168
>50	1.425864922849496E-5	0.0010275522540618746
>100	5.6588124523767106E-5	0.01374402318545842
>500	1.3195683723703596E-4	0.09382647843726208
>1k	5.71434995348302E-4	1.0068873791821173
>5k	9.263882624661756E-5	0.6325139848008712
>10k+	1.0626217673521996E-4	5.842584647391567
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	392572	1.0515622626048076	No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	322935	0.8650292411946943	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	219380	0.5876418317410377	No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	182993	0.49017386140845887	No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	172454	0.4619435885270714	No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	80874	0.21663299070209083	No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	79603	0.2132284288999992	No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	75641	0.20261562491897092	No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	66122	0.1771175731533453	No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	50804	0.1360860407501672	No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	49957	0.1338172257648237	No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	49764	0.1333002466713511	No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	48578	0.13012336996223964	No Hit
CCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	43362	0.11615154119771573	No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	42183	0.11299341502567324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.071459261083121E-5	1.3393240763539012E-5	0.0	5.357296305415605E-6	0.0
2	1.3393240763539012E-5	3.750107413790924E-5	0.0	8.035944458123408E-6	0.0
3	1.3393240763539012E-5	6.696620381769506E-5	2.6786481527078026E-6	8.035944458123408E-6	2.6786481527078026E-6
4	2.4107833374370223E-5	8.839538903935749E-5	2.6786481527078026E-6	1.071459261083121E-5	5.357296305415605E-6
5	3.750107413790924E-5	1.1786051871914331E-4	2.6786481527078026E-6	1.071459261083121E-5	1.875053706895462E-5
6	4.285837044332484E-5	1.3661105578809793E-4	2.6786481527078026E-6	1.3393240763539012E-5	2.142918522166242E-5
7	5.6251611206863856E-5	1.6071888916246814E-4	2.6786481527078026E-6	1.875053706895462E-5	2.142918522166242E-5
8	6.696620381769506E-5	1.7679077807871498E-4	2.6786481527078026E-6	2.142918522166242E-5	2.142918522166242E-5
9	1.071459261083121E-4	1.9554131514766957E-4	2.6786481527078026E-6	2.4107833374370223E-5	2.4107833374370223E-5
10	0.001275036520688914	2.089345559112086E-4	2.6786481527078026E-6	2.6786481527078025E-5	2.6786481527078025E-5
11	0.0015750451137921879	2.3036374113287103E-4	2.6786481527078026E-6	2.9465129679785827E-5	2.9465129679785827E-5
12	0.0016339753731517595	2.3304238928557883E-4	2.6786481527078026E-6	2.9465129679785827E-5	3.750107413790924E-5
13	0.0016714764472896687	2.4375698189641002E-4	2.6786481527078026E-6	3.214377783249363E-5	4.0179722290617036E-5
14	0.001722370762191117	2.598288708126568E-4	2.6786481527078026E-6	3.750107413790924E-5	4.553701859603265E-5
15	0.0030724094311558496	2.7054346342348805E-4	2.6786481527078026E-6	3.750107413790924E-5	4.8215666748740446E-5
16	0.0031393756349735445	2.973299449505661E-4	2.6786481527078026E-6	4.285837044332484E-5	5.6251611206863856E-5
17	0.00327866533891435	3.267950746303519E-4	2.6786481527078026E-6	4.8215666748740446E-5	5.8930259359571654E-5
18	0.0034795639503674355	3.7501074137909234E-4	2.6786481527078026E-6	5.089431490144825E-5	6.964485197040286E-5
19	0.0037929657842342484	3.9108263029533915E-4	2.6786481527078026E-6	5.357296305415605E-5	7.768079642852628E-5
20	0.03394650803926598	4.151904636697094E-4	2.6786481527078026E-6	5.8930259359571654E-5	8.035944458123407E-5
21	0.03465099250342813	4.446555933494952E-4	2.6786481527078026E-6	6.696620381769506E-5	8.571674088664968E-5
22	0.03486260570749205	4.5537018596032643E-4	2.6786481527078026E-6	6.696620381769506E-5	9.10740371920653E-5
23	0.03506886161525055	4.7679937118198887E-4	2.6786481527078026E-6	6.696620381769506E-5	9.375268534477309E-5
24	0.03530993994899426	4.848353156401123E-4	2.6786481527078026E-6	7.500214827581847E-5	9.91099816501887E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	3915	0.0	10.511157	21
CGGTTCA	4515	0.0	10.439422	1
CGAGATC	4360	0.0	9.746005	18
AGATCGG	4320	0.0	9.630878	20
GAGATCG	4530	0.0	9.14756	19
AATGCCG	5070	0.0	9.064267	13
AGCGGTT	4725	0.0	7.830042	30
ATGCCGA	6250	0.0	7.3067846	14
CCGAGAT	6170	0.0	6.9831066	17
TGCCGAG	6545	0.0	6.9624567	15
ATCGGAA	6250	0.0	6.926495	22
TCGGAAG	6405	0.0	6.825864	23
AGTGGTA	13665	0.0	6.7641897	2
GCCGAGA	6780	0.0	6.755165	16
GAGCGGT	5750	0.0	6.720158	29
AAGAGCG	6150	0.0	6.5763183	27
AAGCAGT	11280	0.0	6.5174785	1
GGTATCA	14985	0.0	6.4778643	5
TCAACGC	14665	0.0	6.4407563	9
GTATCAA	14715	0.0	6.4226165	6
>>END_MODULE