FastQCFastQC Report
Fri 27 May 2016
SRR522049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39195991
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3305420.8433056329663919No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2326500.593555601132779No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1903360.48560068298821685No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1629210.4156573053606426No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN817690.20861572297023948No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN810330.20673797991228238No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN790710.20173236594528254No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN722190.1842509862807143No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN708210.18068429498312724No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN547440.13966734506087625No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN521230.13298043669823273No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN511240.13043170665081538No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN480050.12247426018645632No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN459870.1173257744650467No Hit
CCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN410320.1046841754811098No Hit
TTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN403450.10293144520826121No Hit
GTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN397450.10140067641101357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29300.07.55179921
AAGCAGT121250.07.0439971
CGGTTCA37350.06.95606331
AATGCCG39200.06.73731213
AGATCGG35150.06.678426720
TCAACGC148800.06.56529
CGAGATC36600.06.53161418
CAACGCA153850.06.335108310
AGTGGTA149050.06.33114152
AACGCAG158950.06.15689611
ATCAACG157950.06.150688
GGTATCA155600.06.1504545
GTATCAA157000.06.0722156
ACGCAGA161450.05.93618212
GTGGTAT162150.05.83126973
AGAGTAC147250.05.80595416
TGGTATC168000.05.78741934
GAGATCG41500.05.689372519
CGCAGAG170750.05.545346713
GAGTACA144850.05.404971617
Produced by FastQC (version 0.11.5)