##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522049_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39195991
Sequences flagged as poor quality	0
Sequence length	36
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.438283701004014	35.0	25.0	39.0	2.0	40.0
2	27.46160210619499	35.0	18.0	39.0	2.0	39.0
3	27.21103961882224	35.0	14.0	39.0	2.0	39.0
4	26.922701712019478	35.0	10.0	39.0	2.0	39.0
5	26.659465964261496	35.0	2.0	39.0	2.0	39.0
6	26.07444631263437	35.0	2.0	39.0	2.0	39.0
7	25.817895585290852	35.0	2.0	39.0	2.0	39.0
8	25.593360300547065	34.0	2.0	39.0	2.0	39.0
9	25.37674036612571	33.0	2.0	39.0	2.0	39.0
10	25.206811176173606	33.0	2.0	38.0	2.0	39.0
11	25.049643240299755	33.0	2.0	39.0	2.0	39.0
12	24.877659988237063	33.0	2.0	38.0	2.0	39.0
13	24.7343635475373	33.0	2.0	38.0	2.0	39.0
14	24.6273680132236	33.0	2.0	38.0	2.0	39.0
15	24.48175299356508	33.0	2.0	38.0	2.0	39.0
16	24.299722310886334	33.0	2.0	38.0	2.0	39.0
17	24.127783298041884	33.0	2.0	38.0	2.0	39.0
18	23.967087603423522	33.0	2.0	38.0	2.0	39.0
19	23.822065118853608	33.0	2.0	38.0	2.0	39.0
20	23.661186267748658	33.0	2.0	38.0	2.0	39.0
21	23.45753248080907	32.0	2.0	38.0	2.0	39.0
22	23.285482640303698	32.0	2.0	38.0	2.0	39.0
23	23.110212878658942	31.0	2.0	38.0	2.0	39.0
24	22.96330257346982	31.0	2.0	38.0	2.0	39.0
25	22.788224081386282	31.0	2.0	38.0	2.0	39.0
26	22.509806525876588	31.0	2.0	38.0	2.0	39.0
27	22.275920182755424	31.0	2.0	38.0	2.0	39.0
28	22.234487527053467	31.0	2.0	37.0	2.0	39.0
29	22.042381375176863	30.0	2.0	37.0	2.0	39.0
30	21.859462234288195	30.0	2.0	37.0	2.0	39.0
31	21.766713539657665	30.0	2.0	37.0	2.0	39.0
32	21.49499906763424	29.0	2.0	36.0	2.0	39.0
33	21.22741029816034	29.0	2.0	36.0	2.0	39.0
34	20.99442218465659	29.0	2.0	36.0	2.0	39.0
35	20.76648821049071	29.0	2.0	36.0	2.0	39.0
36	20.64446644556072	29.0	2.0	36.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-5.736980682329001
1	2	-5.421727646319685
1	3	-5.617928897459475
1	4	-5.47703102093579
1	5	-5.370648140018133
1	6	-5.039997620378735
1	7	-4.89521699939505
1	8	-4.864198340062824
1	9	-4.725479627491723
1	10	-4.627812636159884
1	11	-4.502935730448687
1	12	-4.430153409371169
1	13	-4.39277202056471
1	14	-4.3416094363992705
1	15	-4.334466251296266
1	16	-4.414777705945209
1	17	-4.3387845833580485
1	18	-4.275052694915665
1	19	-4.186053229726049
1	20	-4.177138527791691
1	21	-4.151815761924169
1	22	-4.042028674080743
1	23	-3.9688394958369457
1	24	-3.9447005601507144
1	25	-3.941264824430675
1	26	-3.8083659052693832
1	27	-3.7530142701429625
1	28	-3.6847078200277643
1	29	-3.6975164681171044
1	30	-3.5803370635325784
1	31	-3.6857566662617494
1	32	-3.5414521390360854
1	33	-3.5489965989375776
1	34	-3.6670690891356443
1	35	-3.89495577514365
1	36	-7.639276562063882
2	1	-1.0792284686590676
2	2	-0.9863478566429826
2	3	-0.9319501845085547
2	4	-0.8353009343976794
2	5	-0.8726557980645495
2	6	-0.8505972379018623
2	7	-0.8420093770082211
2	8	-0.8783083562601774
2	9	-0.7971993469658507
2	10	-0.7432568103688268
2	11	-0.8103442114577071
2	12	-0.7890084674522058
2	13	-0.7659185129552029
2	14	-0.9657147626432092
2	15	-0.8615630367927167
2	16	-0.7191743506821204
2	17	-0.7573466259314046
2	18	-0.6698088346417883
2	19	-0.6861269176999372
2	20	-0.617613186400991
2	21	-0.5180622210285577
2	22	-0.5335701071406476
2	23	-0.5432164454443758
2	24	-0.5881552897929865
2	25	-0.5303024270869692
2	26	-0.4755142648635271
2	27	-0.5028778339199924
2	28	-0.5763573005548537
2	29	-0.5227292073093395
2	30	-0.5708393504643325
2	31	-0.47020343010163046
2	32	-0.5019132815249634
2	33	-0.4099871098936383
2	34	-0.44106872553695453
2	35	-0.5260026116479608
2	36	-0.4175631620610183
3	1	-1.1481578851369711
3	2	-1.1713479996123297
3	3	-1.0694331722165202
3	4	-1.0006720847949246
3	5	-1.0633786092469393
3	6	-0.9574028328475457
3	7	-0.9930439668200037
3	8	-0.9708765850373915
3	9	-1.0193973061142394
3	10	-0.9051711677525063
3	11	-0.9363116746138687
3	12	-0.8708470742930281
3	13	-1.0359302584810663
3	14	-0.8972378248156296
3	15	-0.883225513822179
3	16	-0.7809706780348158
3	17	-0.8172865804644687
3	18	-0.8357582371446668
3	19	-0.7296416935609429
3	20	-0.7143495271496363
3	21	-0.7138653134138693
3	22	-0.8368146081868559
3	23	-0.857557149816909
3	24	-0.8356438807166064
3	25	-0.7540319544928806
3	26	-0.7105143439329105
3	27	-0.7531834704937559
3	28	-0.9009183358009238
3	29	-0.8503548360041364
3	30	-0.7725319223320248
3	31	-0.7262679509284311
3	32	-0.6939614157451004
3	33	-0.7675816440667589
3	34	-0.6872524930543804
3	35	-0.6390277051950868
3	36	-0.616792192906761
4	1	-0.8396912657380433
4	2	-0.7540232164555078
4	3	-0.6662973774636853
4	4	-0.6288363265707204
4	5	-0.7004747122203945
4	6	-0.646471279428809
4	7	-0.691315662677745
4	8	-0.6258440570354935
4	9	-0.6815104654374515
4	10	-0.6285840726129059
4	11	-0.6190640996124728
4	12	-0.5698916413728057
4	13	-0.699623523338694
4	14	-0.6182450830644406
4	15	-0.5727025637152501
4	16	-0.5439336408457756
4	17	-0.5629843214404744
4	18	-0.5319988975053533
4	19	-0.6228437913695046
4	20	-0.67213524247979
4	21	-0.438076757880399
4	22	-0.6961351306199255
4	23	-0.4035279690192617
4	24	-0.3794788871314516
4	25	-0.43148297768572164
4	26	-0.4613193758777143
4	27	-0.5239976854011985
4	28	-0.4679771792448264
4	29	-0.613614251056724
4	30	-0.49497080348540123
4	31	-0.6558787800038672
4	32	-0.6155733582840774
4	33	-0.6444541669556862
4	34	-0.6301714235472353
4	35	-0.6900534734626547
4	36	-0.5279768584966043
5	1	-0.8355986184972934
5	2	-0.8143978622244212
5	3	-0.7462100107741385
5	4	-0.6494363084455905
5	5	-0.6245139410319034
5	6	-0.7463833015525907
5	7	-0.5770824437728486
5	8	-0.6443414822340969
5	9	-0.6557322491820408
5	10	-0.5086351701136493
5	11	-0.5419641745599755
5	12	-0.5683578330039083
5	13	-0.4570569717253754
5	14	-0.6606034079322463
5	15	-0.41208471334128305
5	16	-0.4249210491086579
5	17	-0.48209130825663493
5	18	-0.25740042154624376
5	19	-0.3360689164536872
5	20	-0.34601829328110867
5	21	-0.31084718748773454
5	22	-0.28663309484080557
5	23	-0.3465809052879649
5	24	-0.18774333639068885
5	25	-0.25473041574496236
5	26	-0.0864033233291508
5	27	-0.1084997615007417
5	28	-0.12898620709622222
5	29	-0.16252968496489117
5	30	-0.27993121065823345
5	31	-0.24345718392335058
5	32	-0.09806750328903746
5	33	-0.07583236009317318
5	34	-0.06412577170680578
5	35	-0.08818156971595315
5	36	-0.06087376445765358
6	1	-0.7309845290351937
6	2	-0.7743107653391519
6	3	-0.6723018871227175
6	4	-0.6929457084117026
6	5	-0.5638141524860245
6	6	-0.612228780717917
6	7	-0.5599947579247235
6	8	-0.6460081422169104
6	9	-0.532040750190081
6	10	-0.5861494659409203
6	11	-0.5128776790043403
6	12	-0.4871923475587856
6	13	-0.5053747218979332
6	14	-0.4841035628429289
6	15	-0.4663920764872316
6	16	-0.2959172153877674
6	17	-0.41039221353440425
6	18	-0.2904502347101072
6	19	-0.21634954906613046
6	20	-0.22751330712863904
6	21	-0.190792764972894
6	22	-0.1503272904225028
6	23	-0.05324230415505582
6	24	-0.2180405213228731
6	25	-0.14474293048953157
6	26	-0.1779653755828967
6	27	-0.08103720325415509
6	28	-0.15504899623015334
6	29	-0.16348411969043752
6	30	-0.2409628369936314
6	31	-0.16358952267010096
6	32	-0.19551468386222837
6	33	-0.13608855666377906
6	34	-0.1430072729056242
6	35	-0.43341784401985706
6	36	-0.12568742763731677
7	1	-0.7676249018538392
7	2	-0.7966324675769805
7	3	-0.8845431418779519
7	4	-0.6937029336521263
7	5	-0.6734139821119101
7	6	-0.6173478024865524
7	7	-0.6079263305863591
7	8	-0.6385871490360522
7	9	-0.6736367129917333
7	10	-0.624311231567944
7	11	-0.5378342503088298
7	12	-0.6280743259029009
7	13	-0.5385945003280703
7	14	-0.5759475181113736
7	15	-0.46449341776578734
7	16	-0.4260269250418851
7	17	-0.3774090170988984
7	18	-0.3241757068927775
7	19	-0.3257149890113453
7	20	-0.39818595080872043
7	21	-0.43962313415856613
7	22	-0.33214087692286043
7	23	-0.40188694259028424
7	24	-0.24944389456327798
7	25	-0.26302974152876857
7	26	-0.37113145276070014
7	27	-0.3103241583597409
7	28	-0.46300079037813546
7	29	-0.2712486171494426
7	30	-0.3548239298934561
7	31	-0.2776650865123713
7	32	-0.1656672062760265
7	33	-0.03925170031671499
7	34	-0.12620472105217928
7	35	-0.07865889415912264
7	36	-0.06090123044598528
8	1	-0.6423475341744336
8	2	-0.6226435747058474
8	3	-0.5191541423681905
8	4	-0.6105269003803748
8	5	-0.8493121680078985
8	6	-0.8111955524935048
8	7	-0.9115497428219399
8	8	-0.8218077731663698
8	9	-0.6554086297161099
8	10	-0.7843093274745456
8	11	-0.6049202441928685
8	12	-0.48177777908516717
8	13	-0.6759463488657325
8	14	-0.6463933795937926
8	15	-0.29892745030445056
8	16	-0.4467553394755299
8	17	-0.33856345667539856
8	18	-0.23999763354145642
8	19	-0.2556356880162589
8	20	-0.25858019221858797
8	21	-0.20113240864483117
8	22	-0.3805616406719068
8	23	-0.03896673071908552
8	24	-0.21010277794643528
8	25	-0.3245060352593079
8	26	-0.043221895487924655
8	27	-0.06413484385058865
8	28	-0.228958497036011
8	29	-0.10148674834407245
8	30	0.037399944074010705
8	31	-0.29744996806035573
8	32	0.0898670927965668
8	33	-0.1130715114423424
8	34	-0.26594638425982353
8	35	0.0796152260839662
8	36	-0.2106126258362977
9	1	-0.481777691855541
9	2	-0.48093064842096567
9	3	-0.4842994178370432
9	4	-0.3582181275234255
9	5	-0.4177723971408298
9	6	-0.4109506817370665
9	7	-0.3742968403882898
9	8	-0.32151505507274436
9	9	-0.4167390240086952
9	10	-0.36159389560321387
9	11	-0.6142213726786352
9	12	-0.5050742033465774
9	13	-0.3218498098930098
9	14	-0.23561904963670344
9	15	-0.27197525502622
9	16	-0.09902442356785812
9	17	-0.16192150374807568
9	18	-0.38627100873919673
9	19	-0.16054415452755677
9	20	-0.15667738313092627
9	21	-0.17997283455612845
9	22	-0.07494480667542547
9	23	0.042148706548591264
9	24	0.07885047699533132
9	25	-0.08696500595140932
9	26	-0.19828071366193
9	27	-0.1047392968087415
9	28	-0.3000217920442907
9	29	-0.19132889412935228
9	30	-0.08532392355004248
9	31	-0.02049263941882984
9	32	-0.05987668444205596
9	33	0.0584684603019241
9	34	-0.4336253850010259
9	35	-0.03459262543506014
9	36	0.01737777570048138
10	1	-0.471303854954364
10	2	-0.5006447349633696
10	3	-0.3883693353433699
10	4	-0.38652760758192883
10	5	-0.3112262833417603
10	6	-0.3938407173538039
10	7	-0.45037628730466395
10	8	-0.34509940510971404
10	9	-0.3093976029947747
10	10	-0.24484665352215984
10	11	-0.29554941299224424
10	12	-0.09639437744051449
10	13	-0.2729787646762283
10	14	-0.11809118545761876
10	15	-0.11444875585483416
10	16	-0.2958064202534061
10	17	-0.12922878385841585
10	18	-0.1450301058994441
10	19	-0.00396697030999249
10	20	-0.008394185961421385
10	21	-0.013777596171845374
10	22	-0.14975262499733688
10	23	-0.25602683690629036
10	24	-0.18740704702167577
10	25	0.04828643780810893
10	26	0.12428555226822624
10	27	0.026435927334933496
10	28	0.07313574651869459
10	29	-0.017197953259600496
10	30	0.10034190155216649
10	31	0.03521814187966399
10	32	0.08381908095293156
10	33	-0.14201015317753019
10	34	0.16261221617143562
10	35	0.177891383519583
10	36	0.2655876647867963
11	1	-0.4152754934768659
11	2	-0.3377961833327099
11	3	-0.25338824868246235
11	4	-0.28428460553061896
11	5	-0.26609536208576756
11	6	-0.2085791413572835
11	7	-0.24653269788766963
11	8	-0.22578691447578336
11	9	-0.13575484455050102
11	10	-0.21508772237745433
11	11	-0.21751527335375087
11	12	-0.20807678835921095
11	13	-0.23407689831408973
11	14	-0.2809976694811738
11	15	-0.15409679937343412
11	16	-0.03532055793965583
11	17	-0.05920597992266252
11	18	-0.012755771417648276
11	19	-0.07640190449522066
11	20	-0.09212851594353921
11	21	0.03649284096773897
11	22	0.07370286500291101
11	23	0.0061280206124259
11	24	-0.022623315389914467
11	25	-0.024540135224999204
11	26	-0.11228732608684311
11	27	-0.0038111305129433504
11	28	0.05547450102779905
11	29	0.04542378908488942
11	30	-0.057765386329634794
11	31	-0.024073016936171143
11	32	0.03292739164567493
11	33	0.11338427184810129
11	34	0.13184079080839695
11	35	0.06623449988816787
11	36	0.21201785883253876
12	1	-0.21139924243422925
12	2	-0.19095670122656827
12	3	-0.14148328551558365
12	4	-0.0788047135527421
12	5	-0.004005432566952294
12	6	-0.2123449748302093
12	7	-0.02765799188407314
12	8	-0.1004676038778527
12	9	-0.04318041736632949
12	10	-0.06771823768615448
12	11	0.03399842236462902
12	12	-0.1271942893595579
12	13	0.024443273623916184
12	14	0.12152406185911246
12	15	0.0743740376876616
12	16	0.20334023024298986
12	17	0.22482843689795118
12	18	0.20137126068427236
12	19	0.2201495611911639
12	20	0.26293607118466156
12	21	0.26555036146937994
12	22	0.169129552901655
12	23	0.27414618220817033
12	24	0.2735275825953565
12	25	0.2555050495182023
12	26	0.13608499029470522
12	27	0.2543951309213135
12	28	0.1386637426243027
12	29	0.17152764043782298
12	30	0.13027114440512477
12	31	0.267594111662504
12	32	0.1838413886903858
12	33	0.23944765934479406
12	34	0.272481225951438
12	35	0.2274260085792683
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117	14	-0.8975215480785508
117	15	-1.0080817329228857
117	16	-0.7798488114610009
117	17	-0.8331183747284143
117	18	-0.7760560440087083
117	19	-0.7933873760019239
117	20	-0.6992355358507929
117	21	-0.6795547273925706
117	22	-0.6986905013928784
117	23	-0.6754288829739465
117	24	-0.6904870223751303
117	25	-0.7290575513220645
117	26	-0.5286195455645455
117	27	-0.6532610791513385
117	28	-0.6639167020403711
117	29	-0.7834500713208072
117	30	-0.6207805392642491
117	31	-0.5310626716923608
117	32	-0.6998075099012766
117	33	-0.639394497421705
117	34	-0.6293547875616134
117	35	-0.5740643185217245
117	36	-0.5241418479116611
118	1	-1.3837738541848026
118	2	-1.519163141432628
118	3	-1.4720011595118123
118	4	-1.448844225938057
118	5	-1.4709863581048488
118	6	-1.5583038651622765
118	7	-1.4848660683608763
118	8	-1.5460561978349396
118	9	-1.5379957328440916
118	10	-1.5602028261105723
118	11	-1.4398784786025551
118	12	-1.497953355464574
118	13	-1.4566209607072196
118	14	-1.4536904284056327
118	15	-1.4262028954910626
118	16	-1.4905837056657205
118	17	-1.3839541868293956
118	18	-1.4104080161627763
118	19	-1.4299476676427894
118	20	-1.3261262830768743
118	21	-1.2803711856339817
118	22	-1.3310066288873266
118	23	-1.2659306749540384
118	24	-1.3672943676220122
118	25	-1.5113452192486108
118	26	-1.5099730548253554
118	27	-1.3290553363221562
118	28	-1.4206331080724226
118	29	-1.4792199651944884
118	30	-1.4313011080561218
118	31	-1.3098503442836247
118	32	-1.276380917373146
118	33	-1.6612734788857786
118	34	-1.3024304207058215
118	35	-1.4698545627113866
118	36	-1.2396632279349902
119	1	-1.5833341818588096
119	2	-1.627062974334148
119	3	-1.5850500508444867
119	4	-1.634081856495179
119	5	-1.6535680270303317
119	6	-1.6991666045006681
119	7	-1.7136143507275179
119	8	-1.6696147055623953
119	9	-1.6373378185070209
119	10	-1.7160387026824466
119	11	-1.6289599753073638
119	12	-1.6206209152047641
119	13	-1.689891747533565
119	14	-1.5879190130652923
119	15	-1.5004604519018265
119	16	-1.5833069770954324
119	17	-1.536217804153491
119	18	-1.5274157355167723
119	19	-1.456610119824763
119	20	-1.4476200765991827
119	21	-1.3869483689818338
119	22	-1.341733206744923
119	23	-1.3927643808659376
119	24	-1.4232867846856045
119	25	-1.4812652973568312
119	26	-1.5042286721201812
119	27	-1.4252141096719093
119	28	-1.4500336076430465
119	29	-1.5832862286016969
119	30	-1.3905501161538893
119	31	-1.3900102612413754
119	32	-1.4196222359092623
119	33	-1.2831500518041175
119	34	-1.5005030649020874
119	35	-1.42431434643985
119	36	-1.4671235400856162
120	1	-26.335444656064126
120	2	-25.361664329206477
120	3	-25.10928142134996
120	4	-24.826213174890178
120	5	-24.565084441829708
120	6	-23.98606082023803
120	7	-23.73255650810115
120	8	-23.509974267834412
120	9	-23.292548865927554
120	10	-23.12291728527755
120	11	-22.96608868817865
120	12	-22.797509919894708
120	13	-22.652841200373587
120	14	-22.54821083172084
120	15	-22.395848674934655
120	16	-22.216735846298143
120	17	-22.045649799984652
120	18	-21.88562313520411
120	19	-21.742305794655405
120	20	-21.581048945801893
120	21	-21.37436368946125
120	22	-21.204865046244663
120	23	-21.03288944600403
120	24	-20.881442073898736
120	25	-20.708779627737236
120	26	-20.430075467326017
120	27	-20.198637767827197
120	28	-20.158267062567486
120	29	-19.96517783881535
120	30	-19.781456838405063
120	31	-19.687456848005315
120	32	-19.423348134814944
120	33	-19.15443718051699
120	34	-18.921920469800472
120	35	-18.698936545501862
120	36	-18.577571690163783
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	8716190.0
3	545066.0
4	546895.0
5	603990.0
6	550616.0
7	245461.0
8	268262.0
9	318477.0
10	243384.0
11	183693.0
12	195313.0
13	214907.0
14	207028.0
15	182490.0
16	186976.0
17	195577.0
18	199887.0
19	203074.0
20	225988.0
21	262764.0
22	301286.0
23	338220.0
24	378072.0
25	423442.0
26	472465.0
27	525966.0
28	591778.0
29	682088.0
30	803270.0
31	969890.0
32	1198079.0
33	1527673.0
34	1995123.0
35	2606171.0
36	3317643.0
37	3845839.0
38	3205612.0
39	1717030.0
40	306.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.08647719236704	29.997148307301142	22.64055859590986	28.275815904421957
2	25.431802880216186	29.54631319650169	24.06655585186196	20.955328071420162
3	23.920930136879747	27.530059696189912	24.547484066694995	24.001526100235342
4	26.553924361139597	26.326542292618626	23.467619932758954	23.651913413482824
5	26.840291259344713	27.62193839145875	24.298761277597734	21.2390090715988
6	25.887410329920957	26.41770470959472	22.19004014350265	25.504844816981674
7	24.83401647011052	25.942830448112637	25.311650958626746	23.9115021231501
8	24.489044643119286	25.576749081540374	25.185846220417176	24.748360054923165
9	23.87565215874994	25.25411594946846	25.523457375862186	25.346774515919417
10	24.21166574857486	26.655899256601067	23.727581063150595	25.404853931673475
11	24.493738427189545	26.018680756202905	24.242864018224775	25.244716798382772
12	24.33944304598312	25.6430616131007	24.84112134571034	25.176373995205843
13	24.232693547523404	25.829352246778157	24.85260212452298	25.085352081175454
14	24.19244294470877	25.941483093510882	24.972094787719108	24.893979174061243
15	23.869365037488404	25.66683687508688	25.060991350575446	25.40280673684927
16	24.005670885225253	25.600796085393174	24.96017075546944	25.433362273912135
17	24.12385850148397	25.435919524507316	25.008333598951708	25.43188837505701
18	23.921847903974115	25.313312436550273	24.96938605739614	25.795453602079473
19	24.162514164854727	25.392055984216604	24.874016851839063	25.571412999089603
20	24.156852550144404	25.36453205504423	24.916827397947877	25.56178799686349
21	24.02391973507125	25.312138289217835	24.788319290759578	25.875622684951338
22	24.084373818977916	25.242672592031646	24.71117190841781	25.961781680572628
23	24.0929540899121	25.320077429485	24.814580564484306	25.77238791611859
24	24.15727095689633	25.157834732248592	24.644271201046074	26.040623109809
25	24.243245083316985	25.157904765286577	24.480867770089443	26.117982381306998
26	24.32826404721466	25.195170809591062	24.612448317720094	25.86411682547418
27	24.333028811347788	24.913299957541135	24.472695452633662	26.280975778477416
28	24.30951769448922	25.091982309575023	24.557815669171426	26.040684326764335
29	24.214859083821846	25.214709943519352	24.628221769489034	25.94220920316977
30	24.12282482998813	24.988008219396317	24.61533333125943	26.273833619356125
31	24.18102234513028	25.03147022632144	24.504697353168662	26.282810075379615
32	24.20585728055288	25.055681651628742	24.571409378169047	26.16705168964933
33	24.096784494680417	24.83334012597528	24.50141555897336	26.568459820370936
34	24.139712520519105	24.904598749343037	24.29950488582048	26.656183844317383
35	24.176333124302623	24.935486092838282	24.373525607346057	26.514655175513035
36	24.120553955541286	24.8626188168622	24.322985352561357	26.693841875035158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	943779.0
1	1125989.5
2	1308200.0
3	1308200.0
4	1039681.5
5	771163.0
6	771163.0
7	601930.5
8	432698.0
9	432698.0
10	342443.5
11	252189.0
12	252189.0
13	209524.5
14	166860.0
15	158173.5
16	149487.0
17	149487.0
18	171829.0
19	194171.0
20	194171.0
21	244212.5
22	294254.0
23	294254.0
24	363887.5
25	433521.0
26	541351.5
27	649182.0
28	649182.0
29	806674.5
30	964167.0
31	964167.0
32	1143449.0
33	1322731.0
34	1322731.0
35	1515017.5
36	1707304.0
37	1707304.0
38	1895250.5
39	2083197.0
40	2279989.5
41	2476782.0
42	2476782.0
43	2670209.0
44	2863636.0
45	2863636.0
46	2992769.5
47	3121903.0
48	3121903.0
49	3186653.5
50	3251404.0
51	3238977.0
52	3226550.0
53	3226550.0
54	3114351.5
55	3002153.0
56	3002153.0
57	2825683.5
58	2649214.0
59	2649214.0
60	2410870.0
61	2172526.0
62	2172526.0
63	1910235.0
64	1647944.0
65	1405508.0
66	1163072.0
67	1163072.0
68	967513.0
69	771954.0
70	771954.0
71	627730.5
72	483507.0
73	483507.0
74	389923.5
75	296340.0
76	235962.0
77	175584.0
78	175584.0
79	139910.5
80	104237.0
81	104237.0
82	81821.5
83	59406.0
84	59406.0
85	44841.0
86	30276.0
87	30276.0
88	22209.0
89	14142.0
90	10103.0
91	6064.0
92	6064.0
93	4218.0
94	2372.0
95	2372.0
96	1809.5
97	1247.0
98	1247.0
99	2011.0
100	2775.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.5521561886265358
2	3.0826800628666335
3	4.749503590813663
4	6.211137766614958
5	7.264753173353878
6	8.059564050823463
7	8.556308730655642
8	9.106780843989887
9	9.434444456322076
10	9.607155997152873
11	9.727903039879768
12	9.799124609453044
13	9.905497733173783
14	9.942419876563397
15	10.065037008504262
16	10.104541048598566
17	10.1926572031308
18	10.26246536284795
19	10.312490887142003
20	10.381263226639684
21	10.395703478960387
22	10.384138520697181
23	10.360893796510975
24	10.45688065394239
25	10.476397956107297
26	10.543103757728693
27	10.570570852513974
28	10.54721642323063
29	10.618091018543199
30	10.606717406379646
31	10.576084171465393
32	10.597836396074282
33	10.671025003552021
34	10.656181648781377
35	10.584013553835137
36	10.600533100438767
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3.9195991E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.88052070290057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.72656555111665	66.85079859654125
2	4.725048108523303	6.887279329908677
3	1.4574184943344137	3.1865225624738827
4	0.664861717050041	1.938218725361262
5	0.35974096785146203	1.3109054527589985
6	0.23574383588241568	1.0308680106965755
7	0.16296132700083998	0.8313694456379808
8	0.11277710620763849	0.6575403379023211
9	0.08952481205171502	0.5872153426342469
>10	0.40867622078128335	5.229072499580781
>50	0.030186086034379046	1.5289237540603657
>100	0.025161798421800663	3.4524127712838553
>500	6.155236067331598E-4	0.27968517144506955
>1k	4.772619973449319E-4	0.7219701695404492
>5k	1.2224655530599144E-4	0.6891920690837159
>10k+	1.1894258478208551E-4	4.8180257610903805
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	330542	0.8433056329663919	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	232650	0.593555601132779	No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	190336	0.48560068298821685	No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	162921	0.4156573053606426	No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	81769	0.20861572297023948	No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	81033	0.20673797991228238	No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	79071	0.20173236594528254	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	72219	0.1842509862807143	No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	70821	0.18068429498312724	No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	54744	0.13966734506087625	No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	52123	0.13298043669823273	No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	51124	0.13043170665081538	No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	48005	0.12247426018645632	No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	45987	0.1173257744650467	No Hit
CCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	41032	0.1046841754811098	No Hit
TTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	40345	0.10293144520826121	No Hit
GTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	39745	0.10140067641101357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7858969301222668E-5	3.316665727369924E-5	0.0	2.5512813287460955E-6	7.653843986238286E-6
2	3.316665727369924E-5	8.674356517736725E-5	2.5512813287460955E-6	1.2756406643730478E-5	7.653843986238286E-6
3	4.592306391742972E-5	1.3521791042354306E-4	2.5512813287460955E-6	1.2756406643730478E-5	1.0205125314984382E-5
4	5.102562657492191E-5	1.9644866231344936E-4	2.5512813287460955E-6	1.2756406643730478E-5	1.0205125314984382E-5
5	5.86794705611602E-5	2.347178822446408E-4	2.5512813287460955E-6	1.7858969301222668E-5	1.7858969301222668E-5
6	6.633331454739848E-5	2.7553838350457833E-4	2.5512813287460955E-6	2.0410250629968764E-5	2.5512813287460956E-5
7	7.398715853363677E-5	2.908460714770549E-4	2.5512813287460955E-6	2.296153195871486E-5	2.806409461620705E-5
8	9.184612783485944E-5	3.0615375944953145E-4	2.5512813287460955E-6	2.296153195871486E-5	2.806409461620705E-5
9	1.122563784648282E-4	3.240127287507541E-4	2.5512813287460955E-6	2.5512813287460956E-5	3.316665727369924E-5
10	8.444741198149577E-4	3.5973066735319945E-4	2.5512813287460955E-6	2.5512813287460956E-5	3.826921993119143E-5
11	0.0010383715007996608	4.056537312706292E-4	2.5512813287460955E-6	3.0615375944953144E-5	4.3371782588683624E-5
12	0.0010613330327583757	4.413716698730745E-4	2.5512813287460955E-6	3.0615375944953144E-5	4.592306391742972E-5
13	0.0011098073780045515	4.643332018317894E-4	2.5512813287460955E-6	3.316665727369924E-5	4.8474345246175815E-5
14	0.0011531791605932352	4.7708960847551985E-4	2.5512813287460955E-6	3.826921993119143E-5	6.123075188990629E-5
15	0.0020155122497094156	4.7708960847551985E-4	2.5512813287460955E-6	4.082050125993753E-5	6.633331454739848E-5
16	0.0020818455642568137	4.923972964479965E-4	2.5512813287460955E-6	4.082050125993753E-5	7.653843986238286E-5
17	0.0022043070680366266	5.33217797707934E-4	2.5512813287460955E-6	4.3371782588683624E-5	7.908972119112897E-5
18	0.0023701403544051226	5.791408616253636E-4	2.5512813287460955E-6	4.3371782588683624E-5	8.164100251987506E-5
19	0.0025589351727323337	6.046536749128247E-4	2.5512813287460955E-6	4.8474345246175815E-5	9.184612783485944E-5
20	0.019774981579110985	6.556793014877465E-4	2.5512813287460955E-6	5.61281892324141E-5	9.949997182109772E-5
21	0.020160225059751645	7.169100533776528E-4	2.5512813287460955E-6	6.633331454739848E-5	1.0205125314984382E-4
22	0.02030819937681892	7.449741479938599E-4	2.5512813287460955E-6	7.398715853363677E-5	1.0715381580733601E-4
23	0.020468930100529924	7.679356799525748E-4	2.5512813287460955E-6	7.398715853363677E-5	1.148076597935743E-4
24	0.02066027620018588	7.959997745687818E-4	2.5512813287460955E-6	7.398715853363677E-5	1.148076597935743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	2930	0.0	7.551799	21
AAGCAGT	12125	0.0	7.043997	1
CGGTTCA	3735	0.0	6.9560633	1
AATGCCG	3920	0.0	6.737312	13
AGATCGG	3515	0.0	6.6784267	20
TCAACGC	14880	0.0	6.5652	9
CGAGATC	3660	0.0	6.531614	18
CAACGCA	15385	0.0	6.3351083	10
AGTGGTA	14905	0.0	6.3311415	2
AACGCAG	15895	0.0	6.156896	11
ATCAACG	15795	0.0	6.15068	8
GGTATCA	15560	0.0	6.150454	5
GTATCAA	15700	0.0	6.072215	6
ACGCAGA	16145	0.0	5.936182	12
GTGGTAT	16215	0.0	5.8312697	3
AGAGTAC	14725	0.0	5.805954	16
TGGTATC	16800	0.0	5.7874193	4
GAGATCG	4150	0.0	5.6893725	19
CGCAGAG	17075	0.0	5.5453467	13
GAGTACA	14485	0.0	5.4049716	17
>>END_MODULE