FastQCFastQC Report
Fri 27 May 2016
SRR522048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43519649
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3563060.8187244341056152No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2641940.6070683152798406No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2044480.46978320068711954No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1518560.34893663779319545No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN888960.20426635334306117No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN884280.2031909770228156No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN832560.19130668999651168No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN779630.17914436763954597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN742170.17053676145228103No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN591340.13587885325086146No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN589280.1354055038449414No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN519810.11944259936471455No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN519120.11928405029185782No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN509370.11704368295801283No Hit
TANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN456010.10478255465709294No Hit
TTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN455720.10471591809024011No Hit
GTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN437270.10047645375081035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA43950.08.97235721
AGATCGG43900.08.91120920
CGGTTCA54000.08.0012351
GAGATCG50100.07.927091619
AATGCCG57050.07.91925913
CGAGATC51550.07.759839518
AAGCAGT136750.07.01635171
AGCGGTT53550.06.612384330
ATGCCGA71300.06.2964114
ATCGGAA66800.06.243310522
TCAACGC167100.06.2072639
TGCCGAG72550.06.17089115
CCGAGAT65900.06.13556117
CAACGCA172550.05.92951910
GGTATCA172700.05.8878585
ATCAACG179300.05.87002048
GCCGAGA75000.05.849869316
GAGCGGT60600.05.843878329
GTATCAA171950.05.8391786
AGTGGTA163800.05.8252232
Produced by FastQC (version 0.11.5)