##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522048_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43519649
Sequences flagged as poor quality	0
Sequence length	36
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.007742870352654	33.0	16.0	38.0	2.0	39.0
2	26.053906914552552	33.0	2.0	38.0	2.0	39.0
3	25.834988191196118	33.0	2.0	38.0	2.0	39.0
4	25.574092199135155	33.0	2.0	38.0	2.0	39.0
5	25.326986989256277	33.0	2.0	38.0	2.0	39.0
6	24.724092489808456	33.0	2.0	38.0	2.0	39.0
7	24.458786099125017	33.0	2.0	38.0	2.0	39.0
8	24.239393773603275	33.0	2.0	38.0	2.0	39.0
9	24.03776291945737	33.0	2.0	38.0	2.0	39.0
10	23.841854744738406	32.0	2.0	38.0	2.0	39.0
11	23.671837174054414	33.0	2.0	38.0	2.0	39.0
12	23.484213441151606	32.0	2.0	38.0	2.0	39.0
13	23.36725934990882	32.0	2.0	38.0	2.0	39.0
14	23.220971956828052	32.0	2.0	38.0	2.0	39.0
15	23.08698211237871	31.0	2.0	38.0	2.0	39.0
16	22.888208749110085	31.0	2.0	38.0	2.0	39.0
17	22.72443006146488	31.0	2.0	38.0	2.0	39.0
18	22.569187816749164	31.0	2.0	38.0	2.0	39.0
19	22.421437796982232	31.0	2.0	38.0	2.0	39.0
20	22.264372352819297	31.0	2.0	38.0	2.0	39.0
21	22.08713126339783	30.0	2.0	38.0	2.0	39.0
22	21.91106931951588	30.0	2.0	38.0	2.0	39.0
23	21.740642209683262	29.0	2.0	38.0	2.0	39.0
24	21.588955623240437	29.0	2.0	37.0	2.0	39.0
25	21.4077822410746	29.0	2.0	37.0	2.0	39.0
26	21.156205028216107	29.0	2.0	37.0	2.0	39.0
27	20.923086006507084	28.0	2.0	36.0	2.0	39.0
28	20.859902937176724	28.0	2.0	36.0	2.0	39.0
29	20.661448119675782	27.0	2.0	36.0	2.0	39.0
30	20.443726005235014	27.0	2.0	36.0	2.0	39.0
31	20.38469836923547	27.0	2.0	36.0	2.0	39.0
32	20.145690467310523	27.0	2.0	36.0	2.0	39.0
33	19.90553715173576	27.0	2.0	36.0	2.0	39.0
34	19.65772155009798	26.0	2.0	35.0	2.0	38.0
35	19.41593885557303	25.0	2.0	35.0	2.0	38.0
36	19.360052007772396	24.0	2.0	36.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-11.816356052868702
1	2	-11.535117431129885
1	3	-11.101300094099129
1	4	-11.33586144506689
1	5	-11.269328907557185
1	6	-10.243395053226141
1	7	-10.03474838898214
1	8	-9.965137510960725
1	9	-9.595633733923332
1	10	-9.537546765129617
1	11	-8.655768822841882
1	12	-8.557374221757655
1	13	-8.443132468196353
1	14	-8.24641796651467
1	15	-8.257811803277995
1	16	-8.222347036962672
1	17	-8.159114102704802
1	18	-7.962629314483467
1	19	-7.884850121725428
1	20	-7.727115920335844
1	21	-7.632993312077193
1	22	-7.55646974599785
1	23	-7.499560094836365
1	24	-7.378594620476266
1	25	-7.2186600172363615
1	26	-7.142549564481778
1	27	-7.060410121581777
1	28	-6.783640260677847
1	29	-6.6034144945090745
1	30	-6.455585082738624
1	31	-6.4072682432681365
1	32	-6.255163567360036
1	33	-6.058011449677714
1	34	-5.996294841642573
1	35	-5.834810582794432
1	36	-5.759749148034642
2	1	-0.8743801619078013
2	2	-0.8452659028136189
2	3	-0.7949014482078773
2	4	-0.8274607583633191
2	5	-0.7593534444748578
2	6	-0.7982618307914429
2	7	-0.7831988616106038
2	8	-0.8119659884644079
2	9	-0.7352411596716166
2	10	-0.6893042231705664
2	11	-0.6938157203665583
2	12	-0.6514933606630784
2	13	-0.6363154986221637
2	14	-0.6372553211391327
2	15	-0.6585734880026095
2	16	-0.5736981118960713
2	17	-0.5833016562154754
2	18	-0.6587837074972889
2	19	-0.5557896930789354
2	20	-0.644653588436789
2	21	-0.49056339691516015
2	22	-0.5922984408919199
2	23	-0.5180697903408742
2	24	-0.5728185293463461
2	25	-0.5563814071605293
2	26	-0.5232659630764971
2	27	-0.4820098127635575
2	28	-0.5261863173678414
2	29	-0.5539436958519595
2	30	-0.491406797326853
2	31	-0.7104850470633899
2	32	-0.6298285340141483
2	33	-0.5498239790628716
2	34	-0.5053421164190794
2	35	-0.5526374137530254
2	36	-0.5145609689259878
3	1	-0.8847292633103301
3	2	-0.878439720924586
3	3	-0.7821004813714971
3	4	-0.8812703145411973
3	5	-0.7527629473007913
3	6	-0.7888407093344334
3	7	-0.8169579875115964
3	8	-0.7130873171497925
3	9	-0.8931652600272066
3	10	-0.6729001087774336
3	11	-0.6819951360030885
3	12	-0.6496610393631421
3	13	-0.687959420331488
3	14	-0.7040346438806999
3	15	-0.6267869824140888
3	16	-0.4061575862252518
3	17	-0.38182203197583675
3	18	-0.42206565834181475
3	19	-0.46720205455281416
3	20	-0.4535897301831895
3	21	-0.4221944327033924
3	22	-0.38753279511641026
3	23	-0.31434173579538793
3	24	-0.3349908202123366
3	25	-0.3926942883557203
3	26	-0.35025945305230977
3	27	-0.3625672407555882
3	28	-0.36628922234030625
3	29	-0.3085656961921863
3	30	-0.26232817004861175
3	31	-0.2948193003214392
3	32	-0.46017983090501247
3	33	-0.3160295058282543
3	34	-0.2636805938480613
3	35	-0.312114361891755
3	36	-0.4671535954073569
4	1	-0.9277234151315703
4	2	-0.9221141639891322
4	3	-0.8360395176450552
4	4	-0.8504389919219904
4	5	-0.857651010375303
4	6	-0.8929788590274725
4	7	-0.8705990006514348
4	8	-0.870839318495424
4	9	-0.8203470459262441
4	10	-0.7878401719523893
4	11	-0.7177006136350812
4	12	-0.7919781366438876
4	13	-0.7884769270142229
4	14	-0.8257932637167045
4	15	-0.6555841787886152
4	16	-0.617218362976157
4	17	-0.6044455199029883
4	18	-0.6035250122212013
4	19	-0.5696671729673852
4	20	-0.5920722347495477
4	21	-0.5473286959770363
4	22	-0.532455297182139
4	23	-0.5363259478038209
4	24	-0.42550750081122146
4	25	-0.48666770643841417
4	26	-0.6605442680132256
4	27	-0.5190560012582246
4	28	-0.44464716718525565
4	29	-0.4294587055559198
4	30	-0.49097157818093606
4	31	-0.5862532484991547
4	32	-0.4641947068818695
4	33	-0.3955140993052346
4	34	-0.5375215823779165
4	35	-0.5771939971540938
4	36	-0.5187366176578614
5	1	-0.7395124607376538
5	2	-0.8206074837322923
5	3	-0.7079760063539169
5	4	-0.8441700890293902
5	5	-0.7665368370952343
5	6	-0.7503831619513726
5	7	-0.7647501958535656
5	8	-0.6638400683498666
5	9	-0.714206308441323
5	10	-0.7845280570551978
5	11	-0.7775661586964446
5	12	-0.7064974872622862
5	13	-0.7002927272406261
5	14	-0.641935802249229
5	15	-0.652209434664595
5	16	-0.6098149830267872
5	17	-0.5523267046430789
5	18	-0.5619724626246239
5	19	-0.5171850183889433
5	20	-0.49575881122414245
5	21	-0.4660721966980397
5	22	-0.4345283844587762
5	23	-0.36661397073749313
5	24	-0.4757244563689156
5	25	-0.49455178482838846
5	26	-0.33989099495428476
5	27	-0.392607841744276
5	28	-0.43539239677891217
5	29	-0.4598695423079988
5	30	-0.37629002019327373
5	31	-0.44302624349496966
5	32	-0.536248973793132
5	33	-0.562968234538797
5	34	-0.579397658661847
5	35	-0.49645001648633524
5	36	-0.41130475687634416
6	1	-0.7920265970902136
6	2	-0.8243095984926505
6	3	-0.8023098581493642
6	4	-0.7820503183087837
6	5	-0.7585500500608902
6	6	-0.7242370699895666
6	7	-0.802067060806209
6	8	-0.7887812174083848
6	9	-0.775103769779971
6	10	-0.6312758285819164
6	11	-0.7096331515085978
6	12	-0.7333476188731893
6	13	-0.6858117133625434
6	14	-0.6971474108255222
6	15	-0.6610256908375369
6	16	-0.4672334537579488
6	17	-0.6383882915532162
6	18	-0.4641894542187437
6	19	-0.5047045466177238
6	20	-0.5698740338305477
6	21	-0.4113125044574346
6	22	-0.42400688342539894
6	23	-0.4137040373170464
6	24	-0.37887975455084444
6	25	-0.3289043989706393
6	26	-0.4692179321692791
6	27	-0.4044962493345494
6	28	-0.31931173680708724
6	29	-0.31137902135994366
6	30	-0.3661092545985234
6	31	-0.35588949720588303
6	32	-0.37385388821533994
6	33	-0.4579494953446108
6	34	-0.27124941800263613
6	35	-0.2069551268065588
6	36	-0.3113065594252937
7	1	-0.8844854412025462
7	2	-0.736774543128508
7	3	-0.6906559048862171
7	4	-0.6367151363057957
7	5	-0.7033176781952726
7	6	-0.5962660381075899
7	7	-0.6385966045942801
7	8	-0.6121601575386393
7	9	-0.5507974922025873
7	10	-0.6075747861246263
7	11	-0.4620945072996676
7	12	-0.45072913652623114
7	13	-0.4304135132334963
7	14	-0.5723202152061582
7	15	-0.5663661000299207
7	16	-0.39567854257447266
7	17	-0.5184146624937114
7	18	-0.3282063749123516
7	19	-0.3900841967602595
7	20	-0.37891972089443726
7	21	-0.252370844182213
7	22	-0.3259249951038008
7	23	-0.20144134831276617
7	24	-0.21829467545363812
7	25	-0.2968897403662112
7	26	-0.2880134740939795
7	27	-0.12997494965068057
7	28	-0.18110386578814897
7	29	-0.07154412103554364
7	30	-0.03040838875059748
7	31	-0.3353309197660046
7	32	-0.22137549471954188
7	33	-0.05194316415781586
7	34	-0.0718014645921663
7	35	-0.03854297539413665
7	36	-0.23709587409589616
8	1	-0.6563731979538971
8	2	-0.8119165773322798
8	3	-0.6514801380951774
8	4	-0.7415595240372852
8	5	-0.6474259706158954
8	6	-0.6190540664728523
8	7	-0.6829653434786209
8	8	-0.6884104425422493
8	9	-0.6634625490818031
8	10	-0.7004656722153655
8	11	-0.7329955805066533
8	12	-0.8962357940479819
8	13	-0.7351612291251897
8	14	-0.5167979322544127
8	15	-0.6807404280350511
8	16	-0.5768430680325096
8	17	-0.5562326194162672
8	18	-0.389652855693857
8	19	-0.41463166048517763
8	20	-0.6098094098096212
8	21	-0.6402980527010627
8	22	-0.40900515930295
8	23	-0.4124092082017903
8	24	-0.6166929428557246
8	25	-0.3852018992442545
8	26	-0.3591343597780856
8	27	-0.3920769722583408
8	28	-0.3823632888815389
8	29	-0.2895025209076252
8	30	-0.6101942114585412
8	31	-0.3694484203911905
8	32	-0.48660721790095707
8	33	-0.6291736972854736
8	34	-0.3272134567008962
8	35	-0.5288336488824612
8	36	-0.4046218368398513
9	1	-0.8006544723373565
9	2	-0.7151346024681651
9	3	-0.6863280386594575
9	4	-0.6955705638726322
9	5	-0.5307623979126177
9	6	-0.5707245316352463
9	7	-0.5721615214269207
9	8	-0.5064297246985312
9	9	-0.41730056417037886
9	10	-0.38581899451192214
9	11	-0.35606371218431576
9	12	-0.3536809662259053
9	13	-0.3925136384622263
9	14	-0.4700562505361461
9	15	-0.32504757901445913
9	16	-0.48759225075533763
9	17	-0.41596135869600914
9	18	-0.36056234730830994
9	19	-0.33904950277151613
9	20	-0.16544745850632125
9	21	-0.1904033424538305
9	22	-0.1469535008640186
9	23	-0.1199098026769434
9	24	-0.3162274385457948
9	25	-0.29587806401585226
9	26	-0.035078207460152555
9	27	-0.08041753257179707
9	28	-0.021723585067697115
9	29	0.15191056970437344
9	30	0.09517610809295718
9	31	0.05353538781566414
9	32	0.06922530963528573
9	33	-0.04874271069187941
9	34	0.10996378981796084
9	35	9.556247930291306E-4
9	36	-0.10915690777567733
10	1	-0.5713849489209899
10	2	-0.4881842521600994
10	3	-0.4433175661998163
10	4	-0.43733431753624785
10	5	-0.3906637113385436
10	6	-0.44990831656070185
10	7	-0.46350211612373826
10	8	-0.44057781803594764
10	9	-0.5556067039283974
10	10	-0.29227558561767353
10	11	-0.3264959258541005
10	12	-0.15232578890826787
10	13	-0.21214731730504965
10	14	-0.232147712158838
10	15	-0.1330931660911716
10	16	-0.10986743946938304
10	17	-0.19171119700977712
10	18	-0.1341886065131881
10	19	-0.1725873379424847
10	20	-0.2519132879637027
10	21	-0.14308201776678686
10	22	-0.20540041107406637
10	23	-0.11312208089269049
10	24	-0.10279320711140016
10	25	-0.07721189861253919
10	26	0.05630908689616376
10	27	-0.05640252831368642
10	28	-0.2257576510967212
10	29	-0.08989908544871383
10	30	-0.06326297594738861
10	31	-0.1588155871942618
10	32	-0.10909679225648361
10	33	-0.1434740379748085
10	34	0.021302875737273297
10	35	-0.039045673126590685
10	36	-0.06420114410676447
11	1	-0.4585536319213297
11	2	-0.3995292903261145
11	3	-0.39699617435351797
11	4	-0.4296980067062215
11	5	-0.3389415141010659
11	6	-0.30468962216150786
11	7	-0.3532803331316927
11	8	-0.26116052938913015
11	9	-0.3462631709758952
11	10	-0.34199126131359847
11	11	-0.18306187407821284
11	12	-0.3250100609519819
11	13	-0.25746651253809816
11	14	-0.264613330013308
11	15	-0.0899124002537306
11	16	-0.07647210933177462
11	17	-0.08664396022702547
11	18	-0.10574644251932952
11	19	-0.037850727206745205
11	20	-0.06268840633702766
11	21	-0.008658509817120574
11	22	-0.030178345824602104
11	23	-0.20066074147666413
11	24	0.019185463603296427
11	25	-0.0020055274754184893
11	26	-0.033781513278608344
11	27	-0.053502880086711
11	28	-0.12569098867572137
11	29	-0.10643159361421084
11	30	-0.08786605966501426
11	31	-0.07940266352814973
11	32	0.022797020449836225
11	33	0.05812471263569918
11	34	0.08520141704554618
11	35	-0.08366239102398865
11	36	-0.0057476571036296775
12	1	-0.4899020595811798
12	2	-0.5105767600076057
12	3	-0.36151895891584473
12	4	-0.3592929851230835
12	5	-0.2707030518687823
12	6	-0.36567998650869527
12	7	-0.4080087748038821
12	8	-0.3039073075878491
12	9	-0.5686157520475703
12	10	-0.5114131819946692
12	11	-0.5752526498343151
12	12	-0.2969341455832577
12	13	-0.345364153097794
12	14	-0.29195821384908527
12	15	-0.2326096211735731
12	16	-0.23987527546431409
12	17	-0.1855251065546284
12	18	-0.1678540499092911
12	19	-0.033840075416925686
12	20	-0.1330150923734834
12	21	-0.15198135950632974
12	22	-0.25876070087070246
12	23	-0.07362316982811024
12	24	-0.10914341603681166
12	25	-0.1299562702825625
12	26	-0.021156744537680794
12	27	0.0029614775221986633
12	28	-0.015814229773486232
12	29	-0.035481497660423855
12	30	0.036644315215543344
12	31	-0.19594090375675677
12	32	-0.19626473221067542
12	33	0.013846506375795542
12	34	-0.0784072689544324
12	35	-0.08236335003794792
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116	36	-0.5209771003658616
117	1	-1.0409423984178012
117	2	-1.0202720649379913
117	3	-1.0343333229750122
117	4	-1.0743592326974039
117	5	-1.0410268052037033
117	6	-1.0257872968780752
117	7	-1.0395756774920066
117	8	-1.0798690966583813
117	9	-1.1276218364360062
117	10	-1.038508139593329
117	11	-1.1028880922567588
117	12	-1.1275539483618395
117	13	-1.0800662103488996
117	14	-1.0830813719840613
117	15	-1.007729328799428
117	16	-1.1522894165609827
117	17	-1.0969865111058787
117	18	-1.003765557185556
117	19	-0.9892895071663652
117	20	-1.0164718516092464
117	21	-1.1867640253762275
117	22	-1.0317593087006678
117	23	-0.8967963466446278
117	24	-1.038925303591352
117	25	-0.9351135232565717
117	26	-0.9083904707610344
117	27	-1.0050017012892951
117	28	-1.0004100899639319
117	29	-0.9934696374589542
117	30	-0.8644462816276572
117	31	-1.1164536394039857
117	32	-1.0730836693194838
117	33	-1.124139148013267
117	34	-1.0750634483313881
117	35	-1.1049134563996823
117	36	-0.9713429755835143
118	1	-0.9861263859614446
118	2	-1.119988413714271
118	3	-1.1272398440417426
118	4	-1.1325914839140907
118	5	-1.234127676222439
118	6	-1.2504649060523434
118	7	-1.2040163252520877
118	8	-1.2509232839281097
118	9	-1.154674199375794
118	10	-1.1676187145948234
118	11	-1.2736591161314514
118	12	-1.1047545642430627
118	13	-1.2522442970383842
118	14	-1.1846344617108073
118	15	-1.0598670062247884
118	16	-1.0543721364139707
118	17	-1.030397434089135
118	18	-1.0765451235751726
118	19	-1.001410207531304
118	20	-0.9704467702583663
118	21	-0.9016210750516933
118	22	-0.878022793920394
118	23	-0.939693004425159
118	24	-0.9647323077248373
118	25	-0.8630007508544182
118	26	-0.997175019275307
118	27	-1.0272840405151946
118	28	-1.1394834887485672
118	29	-0.977553547178367
118	30	-0.9506748635761895
118	31	-1.0123360485984563
118	32	-1.1625027249046838
118	33	-1.0277348162613116
118	34	-0.9493335982865005
118	35	-0.9934824854371058
118	36	-0.9284640177707573
119	1	-1.108159392056244
119	2	-1.0897029480086182
119	3	-1.1135789748850407
119	4	-1.0714748980293933
119	5	-1.1530578712075723
119	6	-1.247878020477927
119	7	-1.2011447025145152
119	8	-1.2215570755275174
119	9	-1.22476582322912
119	10	-1.1547083084388028
119	11	-1.1718011125507637
119	12	-1.0647065832049876
119	13	-1.0499888891243216
119	14	-0.9741601401728914
119	15	-0.9658171797904025
119	16	-0.9377069470186115
119	17	-0.9687275137553506
119	18	-0.9553348841948264
119	19	-0.9107431030050925
119	20	-0.9606952381169833
119	21	-0.8335324108793181
119	22	-0.9049527984201085
119	23	-0.7962822308781874
119	24	-0.8337901077540515
119	25	-0.8865028528004544
119	26	-0.8294278158141246
119	27	-0.811590978221691
119	28	-0.8415416947062475
119	29	-1.0714387633811526
119	30	-0.7649895399822029
119	31	-0.846653565769671
119	32	-0.82028755015358
119	33	-0.9021587905578912
119	34	-0.8793045061348685
119	35	-0.7984365835895808
119	36	-0.876914672137719
120	1	-24.847533159314626
120	2	-23.906739437160493
120	3	-23.687691400920542
120	4	-23.427145509469234
120	5	-23.182397121700667
120	6	-22.584095969195875
120	7	-22.3198084106013
120	8	-22.10153735166164
120	9	-21.9033654287518
120	10	-21.706261562876673
120	11	-21.53891156619968
120	12	-21.35045609010834
120	13	-21.234678238236746
120	14	-21.08330708297368
120	15	-20.95531422121052
120	16	-20.756567893764174
120	17	-20.59380147825239
120	18	-20.436202734293445
120	19	-20.28981115034237
120	20	-20.137026599063727
120	21	-19.957792765908906
120	22	-19.785007039051273
120	23	-19.611297023777595
120	24	-19.462710226097247
120	25	-19.27964880940795
120	26	-19.037070813841737
120	27	-18.805673818458377
120	28	-18.741369110879816
120	29	-18.542198132217443
120	30	-18.32951123623467
120	31	-18.270839956302215
120	32	-18.036041988023403
120	33	-17.791014806336985
120	34	-17.551765058403113
120	35	-17.310887273924887
120	36	-17.258497512374404
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	1.0990555E7
3	595177.0
4	627734.0
5	717446.0
6	610940.0
7	293432.0
8	317956.0
9	375358.0
10	286373.0
11	222109.0
12	234067.0
13	250266.0
14	237577.0
15	216542.0
16	222746.0
17	233345.0
18	237177.0
19	244949.0
20	273871.0
21	320016.0
22	367444.0
23	411949.0
24	458289.0
25	508737.0
26	566975.0
27	630441.0
28	706595.0
29	808729.0
30	942573.0
31	1119145.0
32	1363387.0
33	1702096.0
34	2173942.0
35	2745832.0
36	3359732.0
37	3698493.0
38	2959830.0
39	1487502.0
40	322.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.54900241858587	29.978459996982615	23.211866767593747	27.260670816837763
2	25.527938367544976	29.70020933756071	24.05988490449889	20.711967390395415
3	24.28176542969063	27.658630613563552	24.56645999730363	23.493143959442186
4	26.90671357934024	26.498972226145128	23.326184826952037	23.268129367562597
5	26.984377097923367	27.777850407278095	24.152312612944193	21.085459881854344
6	25.893598728781793	26.557947521035487	22.15191673922646	25.396537010956262
7	24.75738742341766	26.08719859213688	25.28963276224752	23.865781222197942
8	24.607860819029273	25.71409391712981	25.05929770718555	24.618747556655368
9	23.93680651555588	25.426469039826678	25.38969152693501	25.24703291768244
10	24.18182216339544	26.797089159187703	23.734014537577288	25.287074139839564
11	24.477100245686508	26.130517572116318	24.264037886260738	25.128344295936433
12	24.29694652879697	25.743011952690804	24.847588350236123	25.112453168276105
13	24.14934170409909	25.940025960649955	24.90782453806492	25.002807797186037
14	24.066935026731883	26.087260249232802	24.99398040665007	24.851824317385244
15	23.748275438189903	25.774581882568132	25.150822617034763	25.326320062207202
16	23.910742672951955	25.521239855072853	25.039093036951865	25.52892443502333
17	23.95798775135547	25.52534780212862	25.070713525303983	25.445950921211917
18	23.719276566598733	25.474833823934883	25.008729572481098	25.79716003698529
19	24.010593673059184	25.534113358496803	24.933245174547267	25.522047793896746
20	23.98710825903706	25.533334016636942	24.972800980521935	25.50675674380406
21	23.85893801577596	25.492841355324085	24.831647514980325	25.816573113919635
22	23.943366865127867	25.40927987336787	24.750910651842513	25.896442609661747
23	23.948779019442224	25.460721344853326	24.86325770576634	25.72724192993811
24	24.041012675988902	25.24390147105175	24.631609753549608	26.08347609940974
25	24.095528455024244	25.30622059829526	24.484064669528163	26.114186277152335
26	24.15389316666234	25.345619380826946	24.58980283267116	25.910684619839557
27	24.229395186742558	25.033073855283313	24.479244685647068	26.258286272327062
28	24.14810936314273	25.270857798732845	24.5679384385011	26.013094399623327
29	24.101978699800934	25.31285492692904	24.63954135508097	25.945625018189055
30	23.93522771579874	25.086136029549756	24.556677748441054	26.42195850621045
31	24.02420206862466	25.1920481962842	24.531668930578903	26.252080804512236
32	24.02973048169306	25.213987955001894	24.598238032515514	26.15804353078953
33	24.000743224043322	25.008850469887882	24.482312909119447	26.508093396949345
34	24.05585260770696	24.977662115809927	24.353944903167633	26.61254037331548
35	23.999178231239917	25.114739834886834	24.429794086315923	26.45628784755733
36	23.992551619306283	24.973432412387695	24.37214606167627	26.661869906629754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1021259.0
1	1213379.0
2	1405499.0
3	1405499.0
4	1121118.5
5	836738.0
6	836738.0
7	656740.0
8	476742.0
9	476742.0
10	377881.5
11	279021.0
12	279021.0
13	231652.0
14	184283.0
15	176290.0
16	168297.0
17	168297.0
18	193767.0
19	219237.0
20	219237.0
21	276325.5
22	333414.0
23	333414.0
24	412457.5
25	491501.0
26	613011.5
27	734522.0
28	734522.0
29	912573.0
30	1090624.0
31	1090624.0
32	1291226.0
33	1491828.0
34	1491828.0
35	1707172.5
36	1922517.0
37	1922517.0
38	2130834.0
39	2339151.0
40	2558478.5
41	2777806.0
42	2777806.0
43	2986143.0
44	3194480.0
45	3194480.0
46	3338209.5
47	3481939.0
48	3481939.0
49	3553406.5
50	3624874.0
51	3608514.0
52	3592154.0
53	3592154.0
54	3461940.5
55	3331727.0
56	3331727.0
57	3135535.0
58	2939343.0
59	2939343.0
60	2666790.0
61	2394237.0
62	2394237.0
63	2107037.5
64	1819838.0
65	1552863.5
66	1285889.0
67	1285889.0
68	1065275.0
69	844661.0
70	844661.0
71	686052.5
72	527444.0
73	527444.0
74	421704.0
75	315964.0
76	249440.5
77	182917.0
78	182917.0
79	143609.0
80	104301.0
81	104301.0
82	80588.0
83	56875.0
84	56875.0
85	42479.0
86	28083.0
87	28083.0
88	20367.0
89	12651.0
90	8929.0
91	5207.0
92	5207.0
93	3635.5
94	2064.0
95	2064.0
96	1554.5
97	1045.0
98	1045.0
99	1281.0
100	1517.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.2911455237150464
2	2.7254562645944134
3	4.64016610060435
4	5.923216430353103
5	7.021030891126902
6	7.83864318390987
7	8.337087001781656
8	8.777573091180033
9	9.149664787048259
10	9.341084069864626
11	9.493757635775049
12	9.547503473660829
13	9.631663159783296
14	9.741776639788617
15	9.844564693065424
16	9.906467765859048
17	10.008449286895674
18	10.04399415078003
19	10.091455471067793
20	10.101175218577705
21	10.164836118048655
22	10.160539208392972
23	10.202554712700003
24	10.257249547210273
25	10.291346789125068
26	10.308224223040034
27	10.324887960378542
28	10.360315635817743
29	10.385065375871942
30	10.374773473012155
31	10.321949057999067
32	10.334357246309592
33	10.403045759858955
34	10.382990451048904
35	10.376866780336396
36	10.334357246309592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	4.3519649E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.13555016418202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.33976196519681	68.45659055319476
2	4.277394324306451	6.342139630032167
3	1.3970974505625944	3.107237643913021
4	0.6635140177726653	1.9675990699689352
5	0.3523553559851058	1.3061029084626012
6	0.2132815513502325	0.9487047089531835
7	0.15056778783124097	0.7813698051511017
8	0.10488820782491177	0.6220755994267917
9	0.07914421928460874	0.5280660215081221
>10	0.3715620260567246	4.849450685101216
>50	0.025425474098366607	1.3077467977244466
>100	0.023708918955309338	3.3386955800849707
>500	6.272153028121026E-4	0.2897323724398977
>1k	4.4957728901182494E-4	0.7218378879911014
>5k	9.343505024974465E-5	0.538913869483954
>10k+	1.2847313296010448E-4	4.893736866563795
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	356306	0.8187244341056152	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	264194	0.6070683152798406	No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	204448	0.46978320068711954	No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	151856	0.34893663779319545	No Hit
AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	88896	0.20426635334306117	No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	88428	0.2031909770228156	No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	83256	0.19130668999651168	No Hit
TGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	77963	0.17914436763954597	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	74217	0.17053676145228103	No Hit
ATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	59134	0.13587885325086146	No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	58928	0.1354055038449414	No Hit
ACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	51981	0.11944259936471455	No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	51912	0.11928405029185782	No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	50937	0.11704368295801283	No Hit
TANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	45601	0.10478255465709294	No Hit
TTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	45572	0.10471591809024011	No Hit
GTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	43727	0.10047645375081035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.757375180116917E-5	4.595625300194861E-5	0.0	0.0	4.595625300194861E-6
2	3.216937710136403E-5	8.731688070370236E-5	0.0	2.2978126500974306E-6	6.893437950292292E-6
3	3.906281505165632E-5	1.2637969575535869E-4	2.2978126500974306E-6	9.191250600389722E-6	1.1489063250487153E-5
4	4.136062770175375E-5	1.6774032345711243E-4	2.2978126500974306E-6	1.1489063250487153E-5	1.6084688550682014E-5
5	4.595625300194861E-5	1.953140752582816E-4	2.2978126500974306E-6	1.3786875900584584E-5	1.6084688550682014E-5
6	5.0551878302143475E-5	2.320790776598405E-4	2.2978126500974306E-6	2.2978126500974307E-5	2.757375180116917E-5
7	5.744531625243577E-5	2.5046157886062E-4	2.2978126500974306E-6	2.2978126500974307E-5	3.906281505165632E-5
8	7.123219215302035E-5	2.7573751801169167E-4	2.2978126500974306E-6	2.5275939151071738E-5	4.365844035185118E-5
9	1.0340156925438438E-4	2.918222065623737E-4	2.2978126500974306E-6	2.757375180116917E-5	5.0551878302143475E-5
10	7.78958488383029E-4	3.1250252041325056E-4	4.595625300194861E-6	2.98715644512666E-5	5.744531625243577E-5
11	9.466988118401415E-4	3.4696971016471206E-4	6.893437950292292E-6	3.446718975146146E-5	6.663656685282549E-5
12	9.949528774921875E-4	3.9292596316666065E-4	9.191250600389722E-6	4.136062770175375E-5	7.123219215302035E-5
13	0.0010432069431442336	4.0211721376705035E-4	9.191250600389722E-6	4.136062770175375E-5	7.812563010331264E-5
14	0.0010753763202455975	4.2279752761792725E-4	9.191250600389722E-6	4.8254065652046044E-5	8.731688070370236E-5
15	0.002171432954342072	4.411800288187067E-4	9.191250600389722E-6	5.0551878302143475E-5	9.421031865399466E-5
16	0.002240367333844995	4.5956253001948615E-4	9.191250600389722E-6	5.514750360233834E-5	9.421031865399466E-5
17	0.0023874273434512307	5.055187830214347E-4	9.191250600389722E-6	5.514750360233834E-5	1.0110375660428695E-4
18	0.002550572041608148	5.330925348226039E-4	1.1489063250487153E-5	5.744531625243577E-5	1.1029500720467667E-4
19	0.0027596729927670143	5.744531625243576E-4	1.1489063250487153E-5	5.744531625243577E-5	1.194862578050664E-4
20	0.029122477527334836	6.273028534765986E-4	1.1489063250487153E-5	5.97431289025332E-5	1.2637969575535869E-4
21	0.02967395256335822	6.640678558781575E-4	1.1489063250487153E-5	6.433875420272805E-5	1.4016657165594328E-4
22	0.02981871476031436	6.709612938284497E-4	1.1489063250487153E-5	6.663656685282549E-5	1.5165563490643043E-4
23	0.02998875289642157	6.939394203294241E-4	1.1489063250487153E-5	6.893437950292292E-5	1.5165563490643043E-4
24	0.030170280095779264	7.054284835799113E-4	1.1489063250487153E-5	6.893437950292292E-5	1.5165563490643043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	4395	0.0	8.972357	21
AGATCGG	4390	0.0	8.911209	20
CGGTTCA	5400	0.0	8.001235	1
GAGATCG	5010	0.0	7.9270916	19
AATGCCG	5705	0.0	7.919259	13
CGAGATC	5155	0.0	7.7598395	18
AAGCAGT	13675	0.0	7.0163517	1
AGCGGTT	5355	0.0	6.6123843	30
ATGCCGA	7130	0.0	6.29641	14
ATCGGAA	6680	0.0	6.2433105	22
TCAACGC	16710	0.0	6.207263	9
TGCCGAG	7255	0.0	6.170891	15
CCGAGAT	6590	0.0	6.135561	17
CAACGCA	17255	0.0	5.929519	10
GGTATCA	17270	0.0	5.887858	5
ATCAACG	17930	0.0	5.8700204	8
GCCGAGA	7500	0.0	5.8498693	16
GAGCGGT	6060	0.0	5.8438783	29
GTATCAA	17195	0.0	5.839178	6
AGTGGTA	16380	0.0	5.825223	2
>>END_MODULE