##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522047_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25497707 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.02728053153956 40.0 39.0 40.0 38.0 40.0 2 39.02107487547802 40.0 39.0 40.0 38.0 40.0 3 39.00887609226979 40.0 39.0 40.0 38.0 40.0 4 38.99146935055768 40.0 39.0 40.0 38.0 40.0 5 38.949665826813366 40.0 39.0 40.0 38.0 40.0 6 39.07263947303183 40.0 39.0 40.0 38.0 40.0 7 39.055430631468155 40.0 39.0 40.0 38.0 40.0 8 39.060608116643586 40.0 39.0 40.0 38.0 40.0 9 39.05349896757383 40.0 39.0 40.0 38.0 40.0 10 39.03486482137394 40.0 39.0 40.0 38.0 40.0 11 39.13805009995605 40.0 39.0 40.0 38.0 40.0 12 39.109065689710846 40.0 39.0 40.0 38.0 40.0 13 39.08506239404194 40.0 39.0 40.0 38.0 40.0 14 39.070492691754595 40.0 39.0 40.0 38.0 40.0 15 39.05319839152595 40.0 39.0 40.0 38.0 40.0 16 39.05080343106931 40.0 39.0 40.0 38.0 40.0 17 39.012363896094655 40.0 39.0 40.0 38.0 40.0 18 38.98309287184138 40.0 39.0 40.0 38.0 40.0 19 38.95551509788704 40.0 39.0 40.0 38.0 40.0 20 38.92505784931955 40.0 39.0 40.0 37.0 40.0 21 38.86870819403486 40.0 39.0 40.0 37.0 40.0 22 38.824655017017804 40.0 39.0 40.0 37.0 40.0 23 38.77088818221968 40.0 39.0 40.0 36.0 40.0 24 38.72999007322502 40.0 39.0 40.0 36.0 40.0 25 38.62946797529676 40.0 39.0 40.0 36.0 40.0 26 38.52805952315634 40.0 39.0 40.0 36.0 40.0 27 38.47734602174227 40.0 39.0 40.0 35.0 40.0 28 38.421068098398024 40.0 38.0 40.0 35.0 40.0 29 38.36767404221877 40.0 38.0 40.0 35.0 40.0 30 38.326130816390666 40.0 38.0 40.0 35.0 40.0 31 38.23731977153867 40.0 38.0 40.0 35.0 40.0 32 38.152919437030164 40.0 38.0 40.0 35.0 40.0 33 38.28419582984462 40.0 38.0 40.0 35.0 40.0 34 38.24418752635286 40.0 38.0 40.0 35.0 40.0 35 38.186497515247154 40.0 38.0 40.0 35.0 40.0 36 38.20850667081554 40.0 38.0 40.0 35.0 40.0 37 38.13477145219372 40.0 38.0 40.0 35.0 40.0 38 38.334211895995196 40.0 39.0 40.0 35.0 40.0 39 38.301720817483705 40.0 39.0 40.0 35.0 40.0 40 38.26549085374618 40.0 39.0 40.0 35.0 40.0 41 38.33753564585239 40.0 39.0 40.0 36.0 40.0 42 38.28822709430303 40.0 39.0 40.0 35.0 40.0 43 38.23699468348271 40.0 39.0 40.0 35.0 40.0 44 38.1868489586142 40.0 39.0 40.0 35.0 40.0 45 38.1344249504475 40.0 39.0 40.0 35.0 40.0 46 38.04770648592048 40.0 39.0 40.0 35.0 40.0 47 37.99368476545754 40.0 39.0 40.0 35.0 40.0 48 37.91127464912825 40.0 38.0 40.0 35.0 40.0 49 37.838714516564174 40.0 38.0 40.0 35.0 40.0 50 37.73509986603894 40.0 38.0 40.0 34.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.11484224065806359 1 2 -0.13852566831966584 1 3 -0.1105776187500922 1 4 -0.11881573062770912 1 5 -0.12235107319102667 1 6 -0.1274268453989933 1 7 -0.15672735645743074 1 8 -0.21205130058982036 1 9 -0.14503056371995626 1 10 -0.15368282679258272 1 11 -0.1791165854897585 1 12 -0.1677960557868019 1 13 -0.1736945399631864 1 14 -0.17582854133669912 1 15 -0.1978778165826185 1 16 -0.1989297092219644 1 17 -0.18578436617951155 1 18 -0.19160876309764063 1 19 -0.2418030632374979 1 20 -0.21562906219489975 1 21 -0.21422529082135355 1 22 -0.21072351255127586 1 23 -0.22612060523095323 1 24 -0.22399458359765845 1 25 -0.19467757966701527 1 26 -0.2721668996277984 1 27 -0.3351780818119039 1 28 -0.27283190582426897 1 29 -0.3773746485187459 1 30 -0.3088480787840169 1 31 -0.30797166770533835 1 32 -0.31042686949554366 1 33 -0.22340436661022522 1 34 -0.23233199380766223 1 35 -0.23755660219548247 1 36 -0.1855525703276797 1 37 -0.17736748502524335 1 38 -0.1953874463078975 1 39 -0.1909993700761774 1 40 -0.20019057931854434 1 41 -0.18545426751334304 1 42 -0.15925814172447872 1 43 -0.1910908420007047 1 44 -0.17204489355097508 1 45 -0.17653040103324713 1 46 -0.16096743037940087 1 47 -0.11872444983852404 1 48 -0.1187234419073917 1 49 -0.11439177444137982 1 50 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-0.2051404291109904 119 18 -0.2217324957812039 119 19 -0.21160417849012703 119 20 -0.23175736814226156 119 21 -0.2596044759654035 119 22 -0.26157643683036724 119 23 -0.29061865200199577 119 24 -0.25704191648656405 119 25 -0.26879712309364834 119 26 -0.2947792243496892 119 27 -0.28707726242783593 119 28 -0.3516620225931888 119 29 -0.33881006126742363 119 30 -0.3324046272967891 119 31 -0.43649112623396746 119 32 -0.43980332144349177 119 33 -0.3853365893112297 119 34 -0.39490331725616556 119 35 -0.44022929013457457 119 36 -0.4225618547146013 119 37 -0.4449858668102209 119 38 -0.43868949571351123 119 39 -0.46450426924604926 119 40 -0.4372941244851205 119 41 -0.42774640379364115 119 42 -0.43634736618299996 119 43 -0.45080079768331416 119 44 -0.4668685390124949 119 45 -0.4714649670092541 119 46 -0.4980800800910714 119 47 -0.5116067780497886 119 48 -0.514277241897112 119 49 -0.5685711479737137 119 50 -0.539635440340895 120 1 -0.11192798374085555 120 2 -0.11905976922028572 120 3 -0.11438700213919617 120 4 -0.13120195593555906 120 5 -0.10778318858503866 120 6 -0.14399529132860778 120 7 -0.19294147543024565 120 8 -0.16370330674966738 120 9 -0.16131264306273607 120 10 -0.17569287536815636 120 11 -0.19735630140834814 120 12 -0.19262669836831492 120 13 -0.19893198688549063 120 14 -0.20284787202120924 120 15 -0.20931959936213929 120 16 -0.20490937505296358 120 17 -0.2266749615241892 120 18 -0.23297985081173067 120 19 -0.250668380384532 120 20 -0.25658013795347756 120 21 -0.2852388428475052 120 22 -0.2680696189121363 120 23 -0.26653691970818016 120 24 -0.30189857110404006 120 25 -0.2825131703636856 120 26 -0.3052402053221286 120 27 -0.29087750265473034 120 28 -0.329830004614287 120 29 -0.3248943385713332 120 30 -0.37367288255367725 120 31 -0.4239875678426941 120 32 -0.40696279657773715 120 33 -0.34549055512309224 120 34 -0.39639990971634376 120 35 -0.37016584330849867 120 36 -0.3731444576484151 120 37 -0.365661762450209 120 38 -0.33887992540496725 120 39 -0.35968351078761884 120 40 -0.3785207739363372 120 41 -0.3533599272214403 120 42 -0.36822763229795186 120 43 -0.38012713237403517 120 44 -0.38139885975027 120 45 -0.3849339747391767 120 46 -0.41075338692537144 120 47 -0.3825281317226583 120 48 -0.3879545544053755 120 49 -0.3780262092429254 120 50 -0.371657636592154 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12211.0 3 452.0 4 493.0 5 547.0 6 1119.0 7 1674.0 8 1943.0 9 2504.0 10 3466.0 11 5071.0 12 5490.0 13 7227.0 14 9677.0 15 11971.0 16 12813.0 17 14161.0 18 16826.0 19 20495.0 20 24867.0 21 22233.0 22 29825.0 23 38543.0 24 45022.0 25 49292.0 26 66170.0 27 88403.0 28 116598.0 29 132050.0 30 126347.0 31 151228.0 32 186214.0 33 238212.0 34 326722.0 35 480078.0 36 772866.0 37 1430036.0 38 2922027.0 39 1.7356536E7 40 766298.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.625117761416544 28.082034778474068 25.1994506051116 27.093396854997785 2 24.53975540715306 28.867399615059924 25.199408961083403 21.393436016703614 3 23.639925739204706 26.618652414509274 26.029638665155264 23.71178318113076 4 25.240752040958036 25.640674277102644 25.083498684803303 24.035074997136014 5 25.40879682713218 27.31741993864442 26.00052093101526 21.273262303208146 6 25.73078826264652 26.21798893524033 23.531143408307265 24.520079393805883 7 24.31315098255698 26.458379178959113 26.2570395055524 22.971430332931504 8 24.590689664760834 26.062488677903467 25.848187054624166 23.49863460271153 9 23.872448608810195 25.580213938453365 26.27009950345731 24.277237949279126 10 23.671356016444932 27.27471140836311 25.087675530980096 23.966257044211858 11 24.43597379168252 26.483781463172356 25.240846167069066 23.83939857807606 12 23.96208804187765 26.04718926294039 25.94794504462695 24.04277765055501 13 23.915781917173966 26.508944510186737 25.69484385399832 23.880429718640976 14 23.905416279197183 26.857077775660375 25.674053749225372 23.563452195917066 15 23.44474740414893 26.41519882552576 26.270981935748182 23.869071834577124 16 23.907526272852692 26.125804175253876 26.024642137428277 23.94202741446515 17 23.846065844273763 26.074713306573017 25.83439365743751 24.24482719171571 18 23.440699981374795 26.43043156782686 25.79870025175205 24.33016819904629 19 24.339929861143986 26.275198001137905 25.750609652860156 23.634262484857953 20 23.97223013034074 26.27651968861357 25.882119517649176 23.869130663396515 21 23.830452675607262 26.59116759008957 25.820027659742113 23.758352074561056 22 24.04409541610938 26.302867155858365 25.863066824008918 23.789970604023335 23 23.79398661303766 26.10315290306822 26.481140767358742 23.621719716535377 24 23.675987805491687 26.383686187938387 25.816831293888505 24.123494712681417 25 23.947671771676614 26.203863148002032 25.795954523676283 24.05251055664507 26 24.027777080452946 25.96498991772892 26.40672079232279 23.600512209495346 27 24.208265599364438 26.001184148227495 26.07478551824997 23.715764734158096 28 23.878827363096658 26.212401001594017 25.928957269087395 23.979814366221934 29 24.143344824486277 25.977993968831274 26.046586314885623 23.83207489179683 30 23.986223204760005 26.208875895181453 26.00287738227085 23.802023517787692 31 23.993844998732023 26.209568584361477 26.043425593770387 23.753160823136117 32 24.028369873589845 25.975816932069616 25.984210153719502 24.011603040621036 33 24.067743270617395 26.144059854507745 25.934800163171968 23.8533967117029 34 24.110727932906663 25.923185462717917 26.108407945222407 23.857678659153017 35 23.746481170198656 26.063412889064725 26.25337174817728 23.936734192559342 36 23.98768215900475 26.013625921279772 26.16985424455579 23.828837675159697 37 24.115951294416192 25.990288691777746 25.836527618984118 24.05723239482194 38 23.76488877003594 26.66071478019818 25.960518298913087 23.613878150852788 39 24.104848202950247 26.158814900370775 25.875946810915273 23.860390085763704 40 24.012606114881184 25.97519553253183 25.87276074920007 24.13943760338692 41 23.83204274147785 26.534201952353243 25.883059309071974 23.750695997096933 42 24.366443537668978 25.888091391043556 25.855697712341076 23.889767358946397 43 24.038057475845683 25.982930246944424 25.920810352803187 24.058201924406703 44 24.014858249805393 26.35412300625117 25.948566039964916 23.68245270397852 45 23.990100812147425 26.192167289967895 25.863259878429883 23.954472019454794 46 23.833903743470728 26.099196802539115 26.245633864824857 23.821265589165296 47 24.121024080081273 26.094272792528468 26.0491211692117 23.73558195817856 48 23.754226709793542 25.93235899357239 25.747193332906583 24.566220963727485 49 23.865465621162397 26.317586025800278 25.684824926078882 24.132123426958447 50 24.37439782476819 26.097540431828612 25.801748544844127 23.72631319855907 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10067.0 1 6721.0 2 3375.0 3 3173.0 4 2971.0 5 2777.0 6 2583.0 7 2751.0 8 2919.0 9 3406.5 10 3894.0 11 5762.0 12 7630.0 13 10208.5 14 12787.0 15 18819.0 16 24851.0 17 36384.5 18 47918.0 19 64844.0 20 81770.0 21 110157.0 22 138544.0 23 202147.5 24 265751.0 25 287396.0 26 309041.0 27 361318.5 28 413596.0 29 464765.5 30 515935.0 31 588280.0 32 660625.0 33 742845.5 34 825066.0 35 911964.5 36 998863.0 37 1086230.5 38 1173598.0 39 1259570.5 40 1345543.0 41 1408860.5 42 1472178.0 43 1513666.0 44 1555154.0 45 1612480.5 46 1669807.0 47 1712151.5 48 1754496.0 49 1770691.0 50 1786886.0 51 1773581.5 52 1760277.0 53 1719202.0 54 1678127.0 55 1616134.0 56 1554141.0 57 1482636.5 58 1411132.0 59 1327780.0 60 1244428.0 61 1066925.0 62 889422.0 63 761218.5 64 633015.0 65 532217.0 66 431419.0 67 362658.0 68 293897.0 69 244006.5 70 194116.0 71 156144.0 72 118172.0 73 98311.0 74 78450.0 75 63205.0 76 47960.0 77 39879.5 78 31799.0 79 26094.5 80 20390.0 81 16016.0 82 11642.0 83 9348.0 84 7054.0 85 5105.5 86 3157.0 87 2304.5 88 1452.0 89 1196.0 90 940.0 91 739.5 92 539.0 93 341.0 94 143.0 95 108.0 96 73.0 97 55.0 98 37.0 99 57.0 100 77.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5418918650214312 2 9.098857399216329E-4 3 0.0 4 0.0 5 0.0643430407291134 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 2.4708104144423657E-4 24 0.0 25 0.020523414125042695 26 0.01353455038133429 27 0.003525807242196328 28 0.009204749274121002 29 0.008436052700738933 30 0.003612089510637172 31 0.0015413150680569042 32 0.003694449857785251 33 0.0026669064790806486 34 0.007381055872984972 35 0.006510389345990994 36 0.009138076612143985 37 0.006498623582112698 38 0.008428208858153402 39 0.006761392308727997 40 0.009651848301496287 41 0.0074947915904751755 42 0.007812467215189194 43 0.0072516324703237045 44 0.010373481819365168 45 0.005780911985536582 46 0.013640442256238963 47 0.014146370103005734 48 0.010585265569174513 49 0.028865340714755255 50 0.03069295603718405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2.5497707E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.76235910809053 #Duplication Level Percentage of deduplicated Percentage of total 1 76.48036524220417 39.58804130384898 2 12.578509310320115 13.021866319305001 3 4.238858662129111 6.582399728327018 4 2.004306455588516 4.1499052206734754 5 1.1125788035507214 2.8794851782721076 6 0.7278082726295251 2.260384390181312 7 0.5080321896960459 1.8407861249061408 8 0.3608549622124583 1.4942963311982165 9 0.2931415867636736 1.3656330075219074 >10 1.5229511065527552 13.742624961876498 >50 0.08634700393715912 3.138950074037275 >100 0.08376934614111178 8.174645809624488 >500 0.002302000118499684 0.7655273633312458 >1k 1.1439963094855127E-4 0.09270251343329884 >5k 3.0329262482272246E-5 0.1324693581785477 >10k+ 3.0329262482268045E-5 0.7702823152843401 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG 67032 0.2628942280966677 Illumina Paired End PCR Primer 2 (100% over 31bp) GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG 56157 0.22024333403784113 Illumina Paired End PCR Primer 2 (97% over 36bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 48783 0.19132308642498713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.039399072238143E-4 5.8828819391484886E-5 0.0 0.0 1.1765763878296978E-5 2 2.117837498093456E-4 1.0981379619743847E-4 0.0 0.0 1.568768517106264E-5 3 2.1962759239487693E-4 1.5687685171062636E-4 0.0 7.84384258553132E-6 2.3531527756593956E-5 4 3.215975460067841E-4 2.1570567110211126E-4 0.0 1.1765763878296978E-5 3.921921292765659E-5 5 0.001047152985168431 2.4315912015147087E-4 0.0 1.1765763878296978E-5 6.275074068425055E-5 6 0.0010549968277539623 3.019879395429558E-4 0.0 1.1765763878296978E-5 6.275074068425055E-5 7 0.001070684512925025 3.8042636539826894E-4 0.0 1.1765763878296978E-5 7.059458326978186E-5 8 0.001094216040681619 4.118017357403942E-4 0.0 1.568768517106264E-5 7.843842585531318E-5 9 0.0012471709710994796 4.3925518478975386E-4 0.0 1.568768517106264E-5 8.236034714807884E-5 10 0.0020903840490440963 4.823963190101761E-4 0.0 1.9609606463828295E-5 9.412611102637582E-5 11 0.0023962939098798176 5.137716893523014E-4 0.0 2.3531527756593956E-5 1.2157956007573543E-4 12 0.0027924079604491493 5.373032171088954E-4 0.0 2.3531527756593956E-5 1.255014813685011E-4 13 0.0031061616638704023 5.961320365003802E-4 0.0 2.3531527756593956E-5 1.451110878323294E-4 14 0.003290491964630388 6.235854855497398E-4 0.0 2.3531527756593956E-5 1.6472069429615768E-4 15 0.015864171629237093 6.510389345990994E-4 0.0 2.7453449049359616E-5 1.6864261558892336E-4 16 0.01613478419843792 6.824143049412247E-4 0.0 2.7453449049359616E-5 2.0001798593104861E-4 17 0.01711526452162934 7.451650456254753E-4 0.0 3.529729163489093E-5 2.117837498093456E-4 18 0.01794278991440289 7.726184946748349E-4 0.0 3.529729163489093E-5 2.313933562731739E-4 19 0.01964882567675595 8.157596288952572E-4 0.0 4.706305551318791E-5 2.3923719885870522E-4 20 0.294418631447918 9.059638186288672E-4 0.0 4.706305551318791E-5 2.6276872661529915E-4 21 0.3004897656091193 9.608707167275865E-4 0.0 4.706305551318791E-5 2.7453449049359615E-4 22 0.30117610183535326 9.961680083624776E-4 0.0 5.098497680595357E-5 2.980660182501901E-4 23 0.30178399963573194 0.0010314652999973684 0.0 5.098497680595357E-5 3.098317821284871E-4 24 0.3025605400516996 0.0010510749064611966 0.0 5.490689809871923E-5 3.1767562471401843E-4 25 0.303054702134588 0.0010628406703394936 0.0 5.490689809871923E-5 3.33363309885081E-4 26 0.3036312245646246 0.0011059818045599159 0.0 5.490689809871923E-5 3.4905099505614366E-4 27 0.3046313144942798 0.0011334352536092756 0.0 5.8828819391484886E-5 3.4905099505614366E-4 28 0.40424811533052757 0.0011648106239514008 0.0 5.8828819391484886E-5 3.6866060151997195E-4 29 0.4064875323886968 0.0012079517581718231 0.0 5.8828819391484886E-5 3.843482866910346E-4 30 0.41426470231225104 0.0012667805775633078 0.0 5.8828819391484886E-5 4.078798144476286E-4 31 0.6423361912504525 0.0013177655543692615 0.0 6.275074068425055E-5 4.1572365703315987E-4 32 0.6445756083086216 0.001368750531175215 0.0 6.275074068425055E-5 4.706305551318791E-4 33 0.6524547481857879 0.001404047822810106 0.0 6.275074068425055E-5 4.980840041812388E-4 34 0.6532469762869265 0.0014628766422015908 0.0 6.275074068425055E-5 4.980840041812388E-4 35 0.6539097809854039 0.0014903300912509506 0.0 6.667266197701621E-5 5.333812958161297E-4 36 0.6546823994800788 0.0015099396977147788 0.0 7.059458326978186E-5 5.451470596944267E-4 37 0.6555060029515595 0.0015373931467641383 0.0 7.843842585531318E-5 5.608347448654892E-4 38 0.6562433241545995 0.0015962219661556233 0.0 7.843842585531318E-5 5.726005087437863E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 2695 0.0 21.142239 43 GACCGAT 2925 0.0 19.627808 32 GATATCG 2535 0.0 19.437061 36 ACCGATA 2530 0.0 19.30136 33 AAGCAGT 19110 0.0 19.233564 1 GAGTACT 13845 0.0 18.939318 20 CGATATC 2615 0.0 18.67412 35 GTACTTT 15590 0.0 16.8759 22 CGAGATC 18410 0.0 16.509823 18 AGATCGG 18590 0.0 16.412628 20 GAGATCG 18650 0.0 16.32444 19 CCGATAT 3040 0.0 15.918744 34 CCGAGAT 20335 0.0 15.119979 17 AGTACTT 17700 0.0 15.050533 21 TCAACGC 22955 0.0 14.812228 12 CAACGCA 23090 0.0 14.716101 13 TCGTATG 4000 0.0 14.6830435 40 AGCAGTG 24970 0.0 14.648317 2 TGCCGAG 21805 0.0 14.546876 26 ATCGTAT 3515 0.0 14.518548 39 >>END_MODULE