FastQCFastQC Report
Fri 27 May 2016
SRR522046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26396208
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1725260.6536014566940828Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG643190.24366757528202537Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT438790.16623221032354343No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG300580.11387241682593197Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35250.026.08723443
TGCCGAG358900.025.25656326
CGAGATC317300.025.06758329
AGATCGG319000.024.81067731
GAGATCG319600.024.78469830
GCCGAGA365550.024.7249427
ATGCCGA369000.024.57885625
GACCGAT39200.024.0173932
CCGAGAT336750.023.62639628
GATATCG34250.023.18558336
ACCGATA33750.023.13792233
CGATATC35300.022.80750535
AATGCCG410250.022.11817224
CCGATAT37200.021.16978334
GAATGCC456550.019.9571323
GGAATGC472350.019.50845122
GAGACCG51300.018.8241130
TCGTATG50650.018.80538740
ATGCCGT48750.018.77303744
CAGGAAT502200.018.43650220
Produced by FastQC (version 0.11.5)