##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522046_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26396208
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.99944238202699	40.0	39.0	40.0	38.0	40.0
2	38.979831837967026	40.0	39.0	40.0	38.0	40.0
3	38.9786054875761	40.0	39.0	40.0	38.0	40.0
4	38.96133065779751	40.0	39.0	40.0	38.0	40.0
5	38.91904458397964	40.0	39.0	40.0	38.0	40.0
6	39.04359989889457	40.0	39.0	40.0	38.0	40.0
7	39.02662295281201	40.0	39.0	40.0	38.0	40.0
8	39.02797663209807	40.0	39.0	40.0	38.0	40.0
9	39.022491791245166	40.0	39.0	40.0	38.0	40.0
10	38.999808987715205	40.0	39.0	40.0	38.0	40.0
11	39.10122211493408	40.0	39.0	40.0	38.0	40.0
12	39.069514378731974	40.0	39.0	40.0	38.0	40.0
13	39.051479250352926	40.0	39.0	40.0	38.0	40.0
14	39.0301403140936	40.0	39.0	40.0	38.0	40.0
15	39.0125879444502	40.0	39.0	40.0	38.0	40.0
16	39.01350436396016	40.0	39.0	40.0	38.0	40.0
17	38.972517491906416	40.0	39.0	40.0	38.0	40.0
18	38.943228550100834	40.0	39.0	40.0	37.0	40.0
19	38.9163213519154	40.0	39.0	40.0	37.0	40.0
20	38.881396714255324	40.0	39.0	40.0	37.0	40.0
21	38.83006339395416	40.0	39.0	40.0	37.0	40.0
22	38.78523441700414	40.0	39.0	40.0	36.0	40.0
23	38.7363122763694	40.0	39.0	40.0	36.0	40.0
24	38.68550065979174	40.0	39.0	40.0	36.0	40.0
25	38.59444504301527	40.0	39.0	40.0	36.0	40.0
26	38.479203528021905	40.0	39.0	40.0	36.0	40.0
27	38.43743722583183	40.0	39.0	40.0	35.0	40.0
28	38.36932998103364	40.0	38.0	40.0	35.0	40.0
29	38.31917345855132	40.0	38.0	40.0	35.0	40.0
30	38.278464732510066	40.0	38.0	40.0	35.0	40.0
31	38.17460769365054	40.0	38.0	40.0	35.0	40.0
32	38.09849600366841	40.0	38.0	40.0	35.0	40.0
33	38.21410454107651	40.0	38.0	40.0	35.0	40.0
34	38.172949463044084	40.0	38.0	40.0	35.0	40.0
35	38.11350588690618	40.0	38.0	40.0	35.0	40.0
36	38.13832350464885	40.0	38.0	40.0	35.0	40.0
37	38.05574543131347	40.0	38.0	40.0	35.0	40.0
38	38.25301365256706	40.0	39.0	40.0	35.0	40.0
39	38.22092597542799	40.0	39.0	40.0	35.0	40.0
40	38.18949778695485	40.0	39.0	40.0	35.0	40.0
41	38.255068455287216	40.0	39.0	40.0	35.0	40.0
42	38.20722033255686	40.0	39.0	40.0	35.0	40.0
43	38.147793008753375	40.0	39.0	40.0	35.0	40.0
44	38.108013620744316	40.0	39.0	40.0	35.0	40.0
45	38.04871389102556	40.0	39.0	40.0	35.0	40.0
46	37.945211751627355	40.0	38.0	40.0	35.0	40.0
47	37.89548623802328	40.0	38.0	40.0	35.0	40.0
48	37.81436113853929	40.0	38.0	40.0	35.0	40.0
49	37.7517413107216	40.0	38.0	40.0	35.0	40.0
50	37.64941066535011	40.0	38.0	40.0	34.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.22783026076905344
1	2	-0.2966871941709499
1	3	-0.24790740515194187
1	4	-0.2715711382932895
1	5	-0.28349710971948383
1	6	-0.22201871028417486
1	7	-0.2664735346520928
1	8	-0.22858827005320137
1	9	-0.2614345353666394
1	10	-0.24650594997568476
1	11	-0.25989900415893175
1	12	-0.2530544790222038
1	13	-0.2325544785977911
1	14	-0.24322143213193925
1	15	-0.24646542274039973
1	16	-0.23922825848298856
1	17	-0.238174731218308
1	18	-0.2234227852897135
1	19	-0.29428198418484186
1	20	-0.24259183152221908
1	21	-0.24931392264545593
1	22	-0.26963781049088453
1	23	-0.2728428385075574
1	24	-0.2967205527021406
1	25	-0.31152759935594787
1	26	-0.2797933958600751
1	27	-0.2718529569730137
1	28	-0.3248367605938469
1	29	-0.42031028319164676
1	30	-0.36608644929834355
1	31	-0.2665341673390458
1	32	-0.3122479442079822
1	33	-0.19095812583226746
1	34	-0.2045492803448994
1	35	-0.17228472935477157
1	36	-0.1668238608676802
1	37	-0.15217040018357153
1	38	-0.11050849268142571
1	39	-0.1744778674414249
1	40	-0.16455555241611108
1	41	-0.10361372148185666
1	42	-0.1336211797078093
1	43	-0.10805116722002595
1	44	-0.13253011544095727
1	45	-0.12657791810607222
1	46	-0.05491551482150214
1	47	-0.05747347231591249
1	48	-0.12132107574450401
1	49	-0.13076064594232406
1	50	-0.1376667971824972
2	1	-0.14933094639206246
2	2	-0.1417128243780823
2	3	-0.1565667330057039
2	4	-0.15184729619252124
2	5	-0.11130772939981881
2	6	-0.14089812911387156
2	7	-0.1420945074137947
2	8	-0.1456131646017127
2	9	-0.16414408892154597
2	10	-0.14210917265499035
2	11	-0.14673191267259966
2	12	-0.15143141287899908
2	13	-0.169561970801702
2	14	-0.16311023717738493
2	15	-0.15613968129581224
2	16	-0.15460246105261888
2	17	-0.17076672621706734
2	18	-0.16553530010237694
2	19	-0.19736580019754513
2	20	-0.16573310540939445
2	21	-0.1957855625034739
2	22	-0.22288865655625045
2	23	-0.2294442734282356
2	24	-0.24946138333953627
2	25	-0.20138603916466025
2	26	-0.27216825951788337
2	27	-0.2632779362149975
2	28	-0.22955994778843802
2	29	-0.32401415432465797
2	30	-0.30209160663617496
2	31	-0.25995863888385884
2	32	-0.4115150868117752
2	33	-0.21919107306074181
2	34	-0.18016727801659727
2	35	-0.1955008634610067
2	36	-0.17090748098809883
2	37	-0.1826090868629322
2	38	-0.18253857045491628
2	39	-0.2030281833612122
2	40	-0.19601544839614604
2	41	-0.2557217272944854
2	42	-0.17328826713720247
2	43	-0.15974727761827268
2	44	-0.20810244354933616
2	45	-0.1769399904223974
2	46	-0.21366548632632743
2	47	-0.21205613480508845
2	48	-0.1855490186397759
2	49	-0.23292995020014473
2	50	-0.2460173338771412
3	1	-0.22625208647379225
3	2	-0.22706296643453072
3	3	-0.2134316300296888
3	4	-0.1914354582426796
3	5	-0.18933789932965084
3	6	-0.27033234685241325
3	7	-0.1829625115841509
3	8	-0.20126272886748353
3	9	-0.18212233069674966
3	10	-0.18718783931749527
3	11	-0.18126768484718525
3	12	-0.19194114729673828
3	13	-0.18218913606335008
3	14	-0.19828412973097187
3	15	-0.18187830782990488
3	16	-0.18070153284358526
3	17	-0.1619305377715179
3	18	-0.188734057537566
3	19	-0.18104043679807091
3	20	-0.19261359630106512
3	21	-0.15451092874242534
3	22	-0.16279997737250795
3	23	-0.1899611414193103
3	24	-0.16264824067462058
3	25	-0.2649884239334668
3	26	-0.2343139254085287
3	27	-0.2670202319160211
3	28	-0.3001080292170428
3	29	-0.23972639881535684
3	30	-0.23589467754249682
3	31	-0.2024078919576695
3	32	-0.23606350444910618
3	33	-0.17245092391844707
3	34	-0.13166596485040571
3	35	-0.11581361101641363
3	36	-0.09652357247408361
3	37	-0.10603900463009808
3	38	-0.10516487653006834
3	39	-0.08384519261013423
3	40	-0.1177784678712257
3	41	-0.12509100057019396
3	42	-0.08039765901858686
3	43	-0.08835236509160183
3	44	-0.08308139378622315
3	45	-0.07772750154699537
3	46	-0.060756852426116836
3	47	-0.0813716110119529
3	48	-0.06355809137988189
3	49	-0.15873345021014984
3	50	-0.08581091126227847
4	1	-0.1485206944609132
4	2	-0.21976115270663144
4	3	-0.2243428808859349
4	4	-0.1804076425270864
4	5	-0.17572187143606044
4	6	-0.1381023571427562
4	7	-0.10588260082558776
4	8	-0.12404486100778911
4	9	-0.1382681278574367
4	10	-0.12469651681158922
4	11	-0.13163787940402472
4	12	-0.15646956927824363
4	13	-0.13734026492107176
4	14	-0.1689667638905945
4	15	-0.13883648364834045
4	16	-0.14931446997599807
4	17	-0.1503310147885628
4	18	-0.1532925610782101
4	19	-0.14371566952579684
4	20	-0.14565258100540746
4	21	-0.13215392681419047
4	22	-0.13220479633675808
4	23	-0.16331988984988755
4	24	-0.14670530123998304
4	25	-0.14623537628781946
4	26	-0.18001668277863558
4	27	-0.15374571058989517
4	28	-0.20202074470880405
4	29	-0.18474730961345642
4	30	-0.17675808418495365
4	31	-0.1658644979922812
4	32	-0.16147953389391034
4	33	-0.09730064696694996
4	34	-0.10938994130651736
4	35	-0.11672716851437315
4	36	-0.06398890805671442
4	37	-0.06946750910967125
4	38	-0.04973094129019984
4	39	-0.06346254483640479
4	40	-0.10004851167661144
4	41	-0.08131354495996135
4	42	-0.06186887521340623
4	43	-0.05224464769771231
4	44	-0.07159158762294737
4	45	-0.05494511402400093
4	46	-0.030925883610741778
4	47	-0.040602888291793704
4	48	-0.045369524617207446
4	49	-0.033214466857756975
4	50	-0.039246571501898586
5	1	-0.15587752113651732
5	2	-0.11640813299565167
5	3	-0.10478601470554594
5	4	-0.1060225771345884
5	5	-0.05716669964041188
5	6	-0.0895560867824301
5	7	-0.1221446692660777
5	8	-0.09935738857561915
5	9	-0.11356106378021735
5	10	-0.11816663501986824
5	11	-0.1237735989015647
5	12	-0.12055036812869702
5	13	-0.1251840381728897
5	14	-0.09995675344086408
5	15	-0.14828723731262983
5	16	-0.10109284738399538
5	17	-0.09637576578370499
5	18	-0.1219637460184515
5	19	-0.12103274965954824
5	20	-0.1266159897021737
5	21	-0.10331567471556014
5	22	-0.13505894602335644
5	23	-0.1035863465102338
5	24	-0.08839763358321306
5	25	-0.10004770337403102
5	26	-0.09279783606618963
5	27	-0.12234770429536468
5	28	-0.09974055242133772
5	29	-0.12537239561223146
5	30	-0.12948822846742303
5	31	-0.10574972451593112
5	32	-0.08484158363650351
5	33	-0.03601547363071944
5	34	0.008224934403884276
5	35	0.001766386368082351
5	36	0.030690999055607904
5	37	0.011926099191683193
5	38	0.025339201557130764
5	39	0.026042833960687517
5	40	0.01226352233864958
5	41	0.005239886670665328
5	42	0.035516826473546814
5	43	0.03181862605010366
5	44	0.04562166900869613
5	45	0.04174275988579268
5	46	0.06767963106119623
5	47	0.04646026046042806
5	48	0.04360100291307134
5	49	0.030910479099652832
5	50	0.01711095910980731
6	1	-0.13162241516477735
6	2	-0.0967864987273046
6	3	-0.10611737551397482
6	4	-0.12664065092604204
6	5	-0.08409879936909448
6	6	-0.11604889289658615
6	7	-0.10738780887300692
6	8	-0.11854930026424171
6	9	-0.1289665598242209
6	10	-0.10844951532941849
6	11	-0.11061168587481518
6	12	-0.12306749833300046
6	13	-0.1384763484321212
6	14	-0.12483304164760511
6	15	-0.11179312839492894
6	16	-0.11217791079333495
6	17	-0.1275583399863649
6	18	-0.10917407741676755
6	19	-0.11420057620922819
6	20	-0.11148902765302893
6	21	-0.10855427736630219
6	22	-0.12762562847466086
6	23	-0.12539976073236403
6	24	-0.15155920376692222
6	25	-0.11276247895313674
6	26	-0.17103555220794675
6	27	-0.14200468775869268
6	28	-0.1571333859213837
6	29	-0.12809847825859322
6	30	-0.1175587972003882
6	31	-0.17805215238487193
6	32	-0.13872539267666895
6	33	-0.08065752004626603
6	34	-0.08051250559338996
6	35	-0.07525389934276205
6	36	-0.03265930362285019
6	37	-0.033775630898169595
6	38	-0.0428214356779506
6	39	-0.05336952216470792
6	40	-0.05442535726692199
6	41	-0.022434676017461186
6	42	-0.003360703863904746
6	43	0.0033470701785276447
6	44	-0.017828447744939524
6	45	-0.018571953399771246
6	46	1.4959125815749985E-4
6	47	7.292848205651126E-4
6	48	-0.0035871595271999013
6	49	-0.031739891337053905
6	50	-0.05325937941964298
7	1	-0.06425481571957192
7	2	-0.06172053756908724
7	3	-0.10502506068892359
7	4	-0.059717642363160905
7	5	-0.051632024322749714
7	6	-0.04477053861676694
7	7	-0.04765590246982754
7	8	-0.050247480561992575
7	9	-0.06817736561487919
7	10	-0.06355434990939557
7	11	-0.04154556505856277
7	12	-0.053730673579295285
7	13	-0.0564980676013036
7	14	-0.04826028645645408
7	15	-0.04982271169480157
7	16	-0.06166089570286459
7	17	-0.06947680265707135
7	18	-0.05033563590206569
7	19	-0.06246452626474763
7	20	-0.08408171235078754
7	21	-0.049372739238194185
7	22	-0.05070935624078032
7	23	-0.0479136096746231
7	24	-0.061199151764014914
7	25	-0.016519871677438402
7	26	-0.02647313295665299
7	27	0.003105997620998835
7	28	-0.013925458600162699
7	29	0.0019246669017789486
7	30	-0.03508948118425792
7	31	0.012810362598173697
7	32	-0.026043814502671125
7	33	0.048185348589328214
7	34	0.04877282550101114
7	35	0.058476972699146756
7	36	0.07272291183793556
7	37	0.05757738322278527
7	38	0.0841844458131078
7	39	0.1045044124148049
7	40	0.09765285383569022
7	41	0.07906123520216113
7	42	0.12516760479932998
7	43	0.0822198234567253
7	44	0.08484107882220115
7	45	0.07101328675577179
7	46	0.10377069393078386
7	47	0.07982173870261988
7	48	0.10978352860210094
7	49	0.10178984468377195
7	50	0.11873378093084597
8	1	-0.0627942399106729
8	2	-0.05647139777217802
8	3	-0.04636252077321501
8	4	-0.07903267654323543
8	5	-0.0709707154610868
8	6	-0.05684804002383004
8	7	-0.05803680113727694
8	8	-0.07767512026538981
8	9	-0.07597706575144514
8	10	-0.061429390100585124
8	11	-0.08887004497668016
8	12	-0.08127748362856835
8	13	-0.08429260655262283
8	14	-0.07720248584247003
8	15	-0.09699373266000322
8	16	-0.09196265941466208
8	17	-0.10122687591507429
8	18	-0.09061862352620409
8	19	-0.09546365262852419
8	20	-0.09749259429857204
8	21	-0.0971853578184465
8	22	-0.0798103628633271
8	23	-0.06826150345628434
8	24	-0.07573993644660959
8	25	-0.06431825480124331
8	26	-0.07103263014009542
8	27	-0.10592916602050195
8	28	-0.13901063163985583
8	29	-0.14238924368930128
8	30	-0.12643627787696943
8	31	-0.14081595680325165
8	32	-0.10015413675998275
8	33	-0.09570454115380045
8	34	-0.03127313111999541
8	35	-0.04978499359025079
8	36	-0.03884234950686505
8	37	0.011566463735476873
8	38	-0.044296640971438705
8	39	-0.025719996772139098
8	40	-0.006538842232387765
8	41	-0.04671654139932002
8	42	-0.0056736547469284915
8	43	-0.027789312070879646
8	44	0.03589846096171101
8	45	0.03373980645899621
8	46	0.00329340331234107
8	47	0.003873479526497192
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113	8	-0.046518165272104284
113	9	-0.04974447178347674
113	10	-0.0754222685156094
113	11	-0.06830529115885042
113	12	-0.08689420669872305
113	13	-0.08856814148654024
113	14	-0.07448490059071844
113	15	-0.09530360055033782
113	16	-0.08986331387272628
113	17	-0.1079859308903579
113	18	-0.10865998097481366
113	19	-0.0934796648495535
113	20	-0.0997662643151358
113	21	-0.12052223492236891
113	22	-0.10945315274375389
113	23	-0.11445553179925128
113	24	-0.10425307880350942
113	25	-0.1105062185744643
113	26	-0.10702856815458972
113	27	-0.08585206228398334
113	28	-0.12206108569447593
113	29	-0.07552347880542953
113	30	-0.1124887834600159
113	31	-0.13614487568007405
113	32	-0.1295632509282143
113	33	-0.08551407670272937
113	34	-0.1088945041024516
113	35	-0.11555913757639757
113	36	-0.10281839248399649
113	37	-0.09860370537353447
113	38	-0.107906759511323
113	39	-0.13579167421870864
113	40	-0.12149389223475993
113	41	-0.1425982526873142
113	42	-0.1606740483058502
113	43	-0.1414971159290701
113	44	-0.1324097980524641
113	45	-0.14155983740208455
113	46	-0.17033542920692923
113	47	-0.14260082674449137
113	48	-0.15784643600635917
113	49	-0.1862117680236608
113	50	-0.13191908504869332
114	1	-0.04909873199046899
114	2	-0.04619403504821662
114	3	-0.046540554575614124
114	4	-0.06244223469190757
114	5	-0.07574987630668062
114	6	-0.08218139243839317
114	7	-0.07366749669579775
114	8	-0.0745286453087246
114	9	-0.0631054022693931
114	10	-0.0743465074438987
114	11	-0.11672629678207613
114	12	-0.09721904504013423
114	13	-0.12042264750385812
114	14	-0.3620107796718486
114	15	-0.11265395963419422
114	16	-0.12490462239712485
114	17	-0.12888784666174757
114	18	-0.13055049562788668
114	19	-0.12575740296482962
114	20	-0.14965817418788419
114	21	-0.14099769946371055
114	22	-0.13420650386758126
114	23	-0.14426521875949305
114	24	-0.1595295051883312
114	25	-0.1182088371492398
114	26	-0.12020475071398806
114	27	-0.10811836140287312
114	28	-0.20131276367806095
114	29	-0.15917651880298678
114	30	-0.13251451946950965
114	31	-0.19201831524321022
114	32	-0.21217140359186715
114	33	-0.15670679656994935
114	34	-0.16289239662662425
114	35	-0.1428684112628673
114	36	-0.15556469653565586
114	37	-0.1689747901667502
114	38	-0.19859828787185307
114	39	-0.1765479800080456
114	40	-0.17973500426553812
114	41	-0.17122740631808142
114	42	-0.17849767348667456
114	43	-0.17026576745272592
114	44	-0.16767966007552104
114	45	-0.18114154549257933
114	46	-0.2105377840129563
114	47	-0.19289416515452018
114	48	-0.21888018660720832
114	49	-0.201319657569222
114	50	-0.21268206015098912
115	1	-0.11264930379935834
115	2	-0.0927396390937858
115	3	-0.060276810322186236
115	4	-0.08169371740842735
115	5	-0.08224365418879387
115	6	-0.06870035747791547
115	7	-0.10830431267009999
115	8	-0.0826431665159646
115	9	-0.09904486814834002
115	10	-0.10754546321977898
115	11	-0.08911061617016003
115	12	-0.09423274232215562
115	13	-0.10157347401693784
115	14	-0.11087330342672175
115	15	-0.10774065986415593
115	16	-0.14096561134572028
115	17	-0.1308676264104065
115	18	-0.15443596613975075
115	19	-0.12553293750123373
115	20	-0.147459365716756
115	21	-0.15665411667799845
115	22	-0.15120511194167818
115	23	-0.1444382490947973
115	24	-0.1303791814127777
115	25	-0.15375575685892073
115	26	-0.1727472484719783
115	27	-0.15074813382186392
115	28	-0.15366035739109662
115	29	-0.17165532987355192
115	30	-0.16710102355617806
115	31	-0.18752807661387294
115	32	-0.20774854017356859
115	33	-0.1260783457805843
115	34	-0.16385150473983856
115	35	-0.15784538164885475
115	36	-0.16117565693783575
115	37	-0.14642894676997997
115	38	-0.1873543204653103
115	39	-0.19501365734453202
115	40	-0.16164174609998838
115	41	-0.20769613806476883
115	42	-0.17655931904553768
115	43	-0.18869777641178587
115	44	-0.24992923562715674
115	45	-0.19404017937380758
115	46	-0.21928536108792684
115	47	-0.22442587448087892
115	48	-0.22277722278035839
115	49	-0.21075047741700814
115	50	-0.21144358878819958
116	1	-0.015474312713365634
116	2	-0.023880794802281002
116	3	0.010717939617052252
116	4	0.01679920163547166
116	5	0.01900124592695107
116	6	-0.010875482721118601
116	7	-0.04165028970979279
116	8	-0.03866329359490095
116	9	-0.029471466170633676
116	10	-0.024686243666224073
116	11	-0.06596917995256746
116	12	-0.06720964413765529
116	13	-0.07251070257605363
116	14	-0.07729741417595193
116	15	-0.07311147976227517
116	16	-0.07718511762104185
116	17	-0.10975197979306728
116	18	-0.10796258416667115
116	19	-0.07575328854944985
116	20	-0.09744289423662167
116	21	-0.09798826292681184
116	22	-0.1189435111520325
116	23	-0.11831629504047214
116	24	-0.11996037821575811
116	25	-0.09157882384143079
116	26	-0.09248074655634753
116	27	-0.10714529858769595
116	28	-0.12364133101572605
116	29	-0.10877000740791232
116	30	-0.1370381152481599
116	31	-0.1453615317600594
116	32	-0.15416510188171628
116	33	-0.1127895268015422
116	34	-0.10621574516827081
116	35	-0.1113436604425928
116	36	-0.09780069453611873
116	37	-0.10980079590517988
116	38	-0.12511309754901845
116	39	-0.12637747147918077
116	40	-0.13467206659013442
116	41	-0.14960830730205998
116	42	-0.12177657203120162
116	43	-0.11268909657432857
116	44	-0.11981455508350791
116	45	-0.1610602789910871
116	46	-0.12666282903897041
116	47	-0.14546772124158736
116	48	-0.15567098840587335
116	49	-0.20554385270727948
116	50	-0.1530775334640353
117	1	-0.105739106626487
117	2	-0.07906064041403482
117	3	-0.1006373822603166
117	4	-0.08497423612561761
117	5	-0.11824671533845077
117	6	-0.08474021756627792
117	7	-0.11600590028016455
117	8	-0.11424379732074641
117	9	-0.12310819535831996
117	10	-0.13238549703851987
117	11	-0.13229263148828352
117	12	-0.15254952403002164
117	13	-0.13700443260174922
117	14	-0.15843224403695189
117	15	-0.15040971386371638
117	16	-0.17146032270300537
117	17	-0.18031948815021792
117	18	-0.178119963456993
117	19	-0.21362838901215042
117	20	-0.2116473618620276
117	21	-0.18013846388940635
117	22	-0.19115050390002608
117	23	-0.19805178884426056
117	24	-0.22223233781380003
117	25	-0.19771695965803815
117	26	-0.25365887618541194
117	27	-0.2374473413824063
117	28	-0.24159062246751972
117	29	-0.2381924684677088
117	30	-0.2750861851673676
117	31	-0.2849749675912676
117	32	-0.3064824152397776
117	33	-0.23960763389107598
117	34	-0.24567644004039835
117	35	-0.260755228233414
117	36	-0.2543442439299781
117	37	-0.2591179795349845
117	38	-0.2703989768353665
117	39	-0.29287047147550993
117	40	-0.27386471992709716
117	41	-0.28366801660200736
117	42	-0.2911855945267092
117	43	-0.3230989221207352
117	44	-0.2764392508716824
117	45	-0.3228183789774093
117	46	-0.365628785621233
117	47	-0.3563533719905365
117	48	-0.33748221794572686
117	49	-0.3696151919988182
117	50	-0.36216516734013027
118	1	-0.07968267281747643
118	2	-0.09349353418769368
118	3	-0.08153184051448648
118	4	-0.06933935272692082
118	5	-0.08050813410365265
118	6	-0.09503242915139509
118	7	-0.11678177536221312
118	8	-0.108133052802458
118	9	-0.12031962114209449
118	10	-0.1266797110840443
118	11	-0.13150063250504473
118	12	-0.14334071807811455
118	13	-0.1463811566434714
118	14	-0.154837752549966
118	15	-0.17642964499413694
118	16	-0.1440621714417034
118	17	-0.17505660551178437
118	18	-0.19346472592820874
118	19	-0.1770525532659306
118	20	-0.1719860562619644
118	21	-0.19711989796402207
118	22	-0.2006645755885117
118	23	-0.2267733659922868
118	24	-0.22493772563299075
118	25	-0.2390755570831331
118	26	-0.18637657796244156
118	27	-0.2499929326731447
118	28	-0.25085367771153955
118	29	-0.2535878219179324
118	30	-0.27856606215994617
118	31	-0.3243907502884227
118	32	-0.3171639047354091
118	33	-0.2769520068415048
118	34	-0.28882235718847227
118	35	-0.3295501584757048
118	36	-0.24997113590307407
118	37	-0.27147150179748536
118	38	-0.2871462561012308
118	39	-0.28625672408404057
118	40	-0.3072669978900535
118	41	-0.28703389236095944
118	42	-0.28701832960820894
118	43	-0.3207656621616266
118	44	-0.3184728814466027
118	45	-0.3444142300908837
118	46	-0.32855261863297613
118	47	-0.3277780605762146
118	48	-0.30770339589816587
118	49	-0.33587105150640895
118	50	-0.32142883379567166
119	1	-0.10810140839542726
119	2	-0.09230718555631512
119	3	-0.10709460361922396
119	4	-0.11097447353940737
119	5	-0.10532849406448719
119	6	-0.13729570715791084
119	7	-0.1310629071935736
119	8	-0.14332116531510763
119	9	-0.13447488334326607
119	10	-0.1479913519685141
119	11	-0.15097975063741131
119	12	-0.16098791189941863
119	13	-0.13609227536205992
119	14	-0.16131466660768012
119	15	-0.1748366201242888
119	16	-0.16473483134825528
119	17	-0.1690572977688305
119	18	-0.1715442794184341
119	19	-0.19086148697125083
119	20	-0.1994384843505017
119	21	-0.20089985155459544
119	22	-0.20869620243912124
119	23	-0.2072535049865607
119	24	-0.24337703468072647
119	25	-0.19883898557757362
119	26	-0.26534157252257273
119	27	-0.2699298596833444
119	28	-0.2510213982197982
119	29	-0.22760002791397227
119	30	-0.27321705689212195
119	31	-0.3180822810896302
119	32	-0.32425085981405033
119	33	-0.28740136364322666
119	34	-0.2813556483117665
119	35	-0.29484962135754245
119	36	-0.30503932648957743
119	37	-0.31720098438451316
119	38	-0.3108846583601377
119	39	-0.31413581545304936
119	40	-0.331086293379812
119	41	-0.3114123228696073
119	42	-0.34161905627057365
119	43	-0.3413869346469909
119	44	-0.350202877220525
119	45	-0.3558545771123818
119	46	-0.3536929949406087
119	47	-0.3371512515187547
119	48	-0.35999224409053454
119	49	-0.3353741558371013
119	50	-0.36487353810787226
120	1	-0.13904203151110295
120	2	-0.15829949900223994
120	3	-0.13266969347138513
120	4	-0.14915248913947465
120	5	-0.13074353719184018
120	6	-0.14396217649350973
120	7	-0.1595274635190762
120	8	-0.1737064210889656
120	9	-0.17188697535022612
120	10	-0.1800215624528292
120	11	-0.19716151001028237
120	12	-0.19238202076125788
120	13	-0.21645224254326934
120	14	-0.22799831444621788
120	15	-0.20457717316602242
120	16	-0.23406855359154832
120	17	-0.21511011201793195
120	18	-0.23631494921055918
120	19	-0.2413147064449106
120	20	-0.2484628453779223
120	21	-0.2329140785366306
120	22	-0.26086202686099824
120	23	-0.271270096485587
120	24	-0.27164592112580976
120	25	-0.2661815745393028
120	26	-0.3164563751033711
120	27	-0.380538300248773
120	28	-0.332012005683616
120	29	-0.30288344509773424
120	30	-0.2993168816624987
120	31	-0.32150045189400345
120	32	-0.37001759025647374
120	33	-0.24656636757531203
120	34	-0.301004936942995
120	35	-0.31189977137538705
120	36	-0.27307673048255054
120	37	-0.2616626853247084
120	38	-0.2507032563088032
120	39	-0.2860296119950547
120	40	-0.2658377320319829
120	41	-0.24246154382742446
120	42	-0.23792651006844068
120	43	-0.24846129555117358
120	44	-0.23226411127652824
120	45	-0.25845554423042216
120	46	-0.2190295894252685
120	47	-0.24798942825212578
120	48	-0.23892473689895866
120	49	-0.20767205619809204
120	50	-0.2301103365478241
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12597.0
3	537.0
4	626.0
5	628.0
6	1116.0
7	1745.0
8	2177.0
9	2702.0
10	3772.0
11	5748.0
12	6113.0
13	8186.0
14	10850.0
15	13283.0
16	13846.0
17	15265.0
18	18334.0
19	21736.0
20	26822.0
21	24297.0
22	32244.0
23	41337.0
24	48685.0
25	53547.0
26	71568.0
27	95364.0
28	125124.0
29	140147.0
30	137666.0
31	164683.0
32	202455.0
33	259611.0
34	355114.0
35	523859.0
36	839416.0
37	1543198.0
38	3095641.0
39	1.7742637E7
40	733532.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.996356946572828	28.04502198609021	24.930036122439954	27.02858494489701
2	24.47159520060476	29.20812084933313	25.192896368903227	21.12738758115888
3	23.42270822808584	26.49034147638772	26.55393037952744	23.533019915999002
4	25.06574429175585	25.57134342932894	25.020188505864176	24.342723773051038
5	25.733232707858665	27.201832396565663	25.915828670723982	21.14910622485169
6	26.0476580575513	26.068873226033073	23.516957435704402	24.36651128071123
7	24.129204467550792	26.874223752138942	26.121592919710285	22.874978860599978
8	24.340181741256167	26.476647706367523	25.768701322553607	23.414469229822707
9	24.21487965241068	25.467839926098474	26.17931712009543	24.137963301395413
10	23.52806888019673	27.65751429144671	24.989987198161188	23.824429630195368
11	24.78636704181146	26.33262701975981	25.208507221946423	23.672498716482306
12	23.783681353018586	25.91980636006505	25.867454143413326	24.429058143503035
13	24.25650684371028	26.373795811883284	25.652627831997687	23.717069512408752
14	24.257419853639583	26.714405341858193	25.584489256941755	23.443685547560467
15	23.264743178262577	26.300929284994268	26.690223838211914	23.744103698531244
16	23.75751471575008	26.013153101384866	26.432902786642686	23.796429396222365
17	23.64928704910948	25.97801169016398	25.751244269631457	24.621456991095084
18	23.25644274359408	26.866730251557346	25.736094366281705	24.140732638566874
19	24.688049889590204	26.134674344133067	25.67695329571581	23.50032247056092
20	23.80940474480274	26.153055772253346	25.81331000270948	24.22422948023443
21	23.665959898482388	26.980136692361267	25.738450765352354	23.615452643803987
22	24.378149315992662	26.20292278345435	25.777115409910394	23.641812490642593
23	24.158366727059253	25.99418567295505	26.372357874867937	23.47508972511776
24	23.532338432853688	26.762203874132222	25.734734322445103	23.970723370568987
25	23.77135284840614	26.607984103015443	25.717234456081993	23.903428592496425
26	23.866990940762008	25.869190630604912	26.804000087748143	23.45981834088494
27	24.54509877314001	25.892795986034162	25.97615002970165	23.585955211124176
28	23.72325507817705	26.131693658429917	25.81904153531836	24.326009728074677
29	24.005780107907825	25.88491375242664	25.934595433929935	24.174710705735595
30	24.332665836899068	26.091172552235463	25.89671613424172	23.679445476623748
31	23.844401431362748	26.601569686193084	25.94298764696487	23.611041235479295
32	24.374726206582466	25.92156643306624	25.851273767579652	23.85243359277164
33	23.90764526227921	26.53433399036954	25.837859447343227	23.72016130000802
34	23.95739183147685	25.80456543153747	26.47309615821778	23.7649465787679
35	23.59073537970038	26.012485543281468	26.10661262343974	24.290166453578415
36	24.31823615374094	25.934252836807016	26.04718331178109	23.700327697670957
37	24.415623906144752	25.960511700062323	25.72216373172955	23.901700662063377
38	23.641557625982973	27.009140376085135	25.876525097712864	23.472776900219035
39	23.988854419866886	26.524384110543203	25.778236264643233	23.708525204946675
40	24.328029397838886	25.8864716057775	25.793583242949953	23.991915753433663
41	23.724877255487602	26.86237289425027	25.76990496376531	23.64284488649682
42	24.69530836426025	25.774750823944252	25.753140910388833	23.776799901406665
43	23.88064270548131	25.8627943050427	25.869030500451274	24.387532489024714
44	24.347442343002086	26.247605979917495	25.848574533449526	23.5563771436309
45	24.28034772108754	26.071489269744646	25.796552688525153	23.851610320642656
46	23.71656795149522	26.020514695504822	26.577664196656155	23.6852531563438
47	24.002176534102464	25.9550441359695	26.3944695634	23.648309766528033
48	23.601704325574733	25.820573380278184	25.658301948541713	24.919420345605374
49	23.769345066898996	26.685574635054003	25.57621974925892	23.968860548788076
50	24.692447750391718	25.977226351498572	25.74538227331031	23.584943624799404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10773.0
1	6939.5
2	3106.0
3	2994.5
4	2883.0
5	2673.5
6	2464.0
7	2657.5
8	2851.0
9	3335.0
10	3819.0
11	5670.5
12	7522.0
13	10299.0
14	13076.0
15	19316.0
16	25556.0
17	37473.5
18	49391.0
19	67451.0
20	85511.0
21	114380.5
22	143250.0
23	205761.0
24	268272.0
25	294911.5
26	321551.0
27	376466.0
28	431381.0
29	484168.5
30	536956.0
31	611102.5
32	685249.0
33	770253.0
34	855257.0
35	946780.0
36	1038303.0
37	1128578.0
38	1218853.0
39	1307164.0
40	1395475.0
41	1460371.0
42	1525267.0
43	1565559.5
44	1605852.0
45	1665349.5
46	1724847.0
47	1771881.5
48	1818916.0
49	1829752.0
50	1840588.0
51	1825109.5
52	1809631.0
53	1764271.5
54	1718912.0
55	1658147.5
56	1597383.0
57	1580317.5
58	1563252.0
59	1417330.5
60	1271409.0
61	1089077.5
62	906746.0
63	775820.5
64	644895.0
65	540822.0
66	436749.0
67	368755.5
68	300762.0
69	249465.5
70	198169.0
71	159731.5
72	121294.0
73	100422.5
74	79551.0
75	64116.5
76	48682.0
77	40416.5
78	32151.0
79	26873.0
80	21595.0
81	17265.0
82	12935.0
83	10471.5
84	8008.0
85	5758.5
86	3509.0
87	2564.5
88	1620.0
89	1310.0
90	1000.0
91	808.5
92	617.0
93	383.0
94	149.0
95	103.5
96	58.0
97	44.5
98	31.0
99	81.0
100	131.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5228591925021958
2	0.0011327384600091043
3	3.523233337152064E-4
4	0.0
5	0.06516845146848366
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.2122953418157639E-4
24	0.0
25	0.02004454579233502
26	0.00942180785967439
27	0.0013941396430881283
28	0.004004363050935196
29	6.099360938510562E-4
30	2.3488222247680424E-4
31	3.939959860901232E-4
32	0.0032239479246412967
33	0.005368195310477929
34	0.00836483785852877
35	0.003943748283844407
36	0.011281923524772952
37	0.006080418823794691
38	0.0038414608643787015
39	0.004436243266457061
40	0.008179205134313233
41	0.006625951727611785
42	0.0034664069930044495
43	0.00723588782146284
44	0.006807796028884149
45	0.0039020756314694896
46	0.01111144449233011
47	0.014354334531687279
48	0.019855124645176307
49	0.02377614239136167
50	0.022950266189749678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.6396208E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.33707119544169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.66596522073893	39.358061148043305
2	12.552305268481678	12.887971784699234
3	4.1006967763476245	6.315532870748288
4	1.968125232024704	4.041511406319897
5	1.1416642103740187	2.9304848424629357
6	0.7065836407172291	2.176436080142087
7	0.5105682107301801	1.834775360906917
8	0.3896604194360961	1.6003219755709226
9	0.2776487131503798	1.2828304578891434
>10	1.5120423068682984	13.540971276550346
>50	0.0870605382223453	3.145955120565719
>100	0.0845277909272297	8.406074675971912
>500	0.002787385534490124	0.9236955651456763
>1k	3.052973309061837E-4	0.22139497002341557
>5k	1.4747278980913411E-5	0.05670166470200657
>10k+	4.424183694270408E-5	1.277280800258123
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	172526	0.6536014566940828	Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	64319	0.24366757528202537	Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	43879	0.16623221032354343	No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG	30058	0.11387241682593197	Illumina Paired End PCR Primer 2 (96% over 33bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7126544843107766E-4	5.682634414761393E-5	0.0	1.1365268829522786E-5	7.576845886348524E-6
2	3.788422943174262E-4	8.334530474983376E-5	0.0	1.5153691772697048E-5	7.576845886348524E-6
3	3.8641914020377474E-4	1.3638322595427342E-4	0.0	1.894211471587131E-5	1.5153691772697048E-5
4	4.8491813672630555E-4	1.8184430127236457E-4	0.0	2.6518960602219834E-5	2.2730537659045572E-5
5	0.0010531815782024447	2.0078641598823588E-4	0.0	3.0307383545394097E-5	3.4095806488568356E-5
6	0.0010872773846910132	2.5382433719267557E-4	0.0	3.788422943174262E-5	4.167265237491688E-5
7	0.0010872773846910132	2.917085666244182E-4	0.0	3.788422943174262E-5	5.303792120443967E-5
8	0.0011478921517818013	3.4095806488568356E-4	0.0	4.167265237491688E-5	5.303792120443967E-5
9	0.001314582761281469	3.788422943174262E-4	0.0	4.5461075318091144E-5	6.0614767090788194E-5
10	0.0022919958806204284	4.091496778628203E-4	0.0	4.9249498261265406E-5	6.440319003396246E-5
11	0.0025950697160743693	4.508223302377372E-4	0.0	4.9249498261265406E-5	7.198003592031098E-5
12	0.0029436046268464014	4.811297137831313E-4	0.0	4.9249498261265406E-5	8.334530474983376E-5
13	0.0032277363475844713	5.152255202716997E-4	0.0	6.819161297713671E-5	9.471057357935654E-5
14	0.0033489658817660475	5.303792120443967E-4	0.0	7.576845886348524E-5	1.0607584240887934E-4
15	0.014589216754164082	5.455329038170937E-4	0.0	7.576845886348524E-5	1.098642653520536E-4
16	0.01490744428139072	5.720518644193135E-4	0.0	7.576845886348524E-5	1.288063800679249E-4
17	0.01583939632541159	6.36455054453276E-4	0.0	7.95568818066595E-5	1.4774849478379622E-4
18	0.016608446182875963	6.819161297713671E-4	0.0	8.713372769300803E-5	1.4774849478379622E-4
19	0.018085931130713925	7.425308968621553E-4	0.0	8.713372769300803E-5	1.5532534067014473E-4
20	0.27348625226775	7.766267033507237E-4	0.0	9.092215063618229E-5	1.59113763613319E-4
21	0.2799038407334872	8.561835851573832E-4	0.0	9.092215063618229E-5	1.7426745538601606E-4
22	0.28054029578794043	8.864909687027773E-4	0.0	1.0228741946570507E-4	1.8563272421553883E-4
23	0.28118432768828006	9.092215063618229E-4	0.0	1.0228741946570507E-4	2.1215168481775868E-4
24	0.28308990442869675	9.243751981345199E-4	0.0	1.0607584240887934E-4	2.197285307041072E-4
25	0.2836695331390024	9.319520440208684E-4	0.0	1.1365268829522786E-4	2.197285307041072E-4
26	0.284313565039342	0.0010077205028843537	0.0	1.2122953418157639E-4	2.197285307041072E-4
27	0.28532128554222635	0.0010531815782024447	0.0	1.2501795712475066E-4	2.3488222247680424E-4
28	0.4050278737006467	0.0011100079223500588	0.0	1.3638322595427342E-4	2.4245906836315278E-4
29	0.4082215142417426	0.0011516805747249757	0.0	1.4396007184062196E-4	2.500359142495013E-4
30	0.4247390382739824	0.0012085069188725896	0.0	1.4774849478379622E-4	2.614011830790241E-4
31	1.1012036274301218	0.0012198721877021124	0.0	1.5153691772697047E-4	2.689780289653726E-4
32	1.10664380277652	0.0013032174924519462	0.0	1.6290218655649327E-4	2.9549698956759244E-4
33	1.1214527480613883	0.0013524669907132116	0.0	1.7047903244284178E-4	3.0307383545394095E-4
34	1.1222899895318297	0.0013941396430881285	0.0	1.7805587832919032E-4	3.068622583971152E-4
35	1.1230628278122372	0.0014282354495766968	0.0	1.8184430127236457E-4	3.2580437311298654E-4
36	1.123839454515588	0.0014585428331220908	0.0	1.8563272421553883E-4	3.3338121899933505E-4
37	1.1247827718284384	0.0014736965248947878	0.0	1.9320957010188737E-4	3.5611175665838064E-4
38	1.1256919933348002	0.0014964270625538335	0.0	1.9320957010188737E-4	3.599001796015549E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	3525	0.0	26.087234	43
TGCCGAG	35890	0.0	25.256563	26
CGAGATC	31730	0.0	25.067583	29
AGATCGG	31900	0.0	24.810677	31
GAGATCG	31960	0.0	24.784698	30
GCCGAGA	36555	0.0	24.72494	27
ATGCCGA	36900	0.0	24.578856	25
GACCGAT	3920	0.0	24.01739	32
CCGAGAT	33675	0.0	23.626396	28
GATATCG	3425	0.0	23.185583	36
ACCGATA	3375	0.0	23.137922	33
CGATATC	3530	0.0	22.807505	35
AATGCCG	41025	0.0	22.118172	24
CCGATAT	3720	0.0	21.169783	34
GAATGCC	45655	0.0	19.95713	23
GGAATGC	47235	0.0	19.508451	22
GAGACCG	5130	0.0	18.82411	30
TCGTATG	5065	0.0	18.805387	40
ATGCCGT	4875	0.0	18.773037	44
CAGGAAT	50220	0.0	18.436502	20
>>END_MODULE