Basic Statistics
Measure | Value |
---|---|
Filename | SRR522045_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 28276936 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG | 92339 | 0.326552353479882 | Illumina Paired End PCR Primer 2 (97% over 36bp) |
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG | 66046 | 0.2335684460296547 | Illumina Paired End PCR Primer 2 (100% over 31bp) |
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 38041 | 0.13453013438231073 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 3320 | 0.0 | 26.172342 | 43 |
ACCGATA | 2985 | 0.0 | 23.728765 | 33 |
GACCGAT | 3745 | 0.0 | 23.670887 | 32 |
GATATCG | 3035 | 0.0 | 23.410986 | 36 |
CGATATC | 3190 | 0.0 | 22.204035 | 35 |
GAGTACT | 11455 | 0.0 | 20.43341 | 20 |
TCAACGC | 14705 | 0.0 | 20.416466 | 12 |
CCGATAT | 3510 | 0.0 | 20.117064 | 34 |
ATGCCGA | 25985 | 0.0 | 20.097977 | 25 |
TGCCGAG | 26020 | 0.0 | 20.059685 | 26 |
GCCGAGA | 26035 | 0.0 | 19.929144 | 27 |
CAACGCA | 15030 | 0.0 | 19.770119 | 13 |
CGAGATC | 21945 | 0.0 | 19.324736 | 29 |
AGATCGG | 22230 | 0.0 | 19.09748 | 31 |
GAGATCG | 22640 | 0.0 | 18.800014 | 30 |
GAGACCG | 4935 | 0.0 | 18.675856 | 30 |
ATCAACG | 16390 | 0.0 | 18.424873 | 11 |
GGTATCA | 16765 | 0.0 | 18.091461 | 8 |
AGACCGA | 5010 | 0.0 | 18.08913 | 31 |
GTATCAA | 16835 | 0.0 | 18.042366 | 9 |