FastQCFastQC Report
Fri 27 May 2016
SRR522045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28276936
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG923390.326552353479882Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG660460.2335684460296547Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380410.13453013438231073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33200.026.17234243
ACCGATA29850.023.72876533
GACCGAT37450.023.67088732
GATATCG30350.023.41098636
CGATATC31900.022.20403535
GAGTACT114550.020.4334120
TCAACGC147050.020.41646612
CCGATAT35100.020.11706434
ATGCCGA259850.020.09797725
TGCCGAG260200.020.05968526
GCCGAGA260350.019.92914427
CAACGCA150300.019.77011913
CGAGATC219450.019.32473629
AGATCGG222300.019.0974831
GAGATCG226400.018.80001430
GAGACCG49350.018.67585630
ATCAACG163900.018.42487311
GGTATCA167650.018.0914618
AGACCGA50100.018.0891331
GTATCAA168350.018.0423669
Produced by FastQC (version 0.11.5)