FastQCFastQC Report
Fri 27 May 2016
SRR522044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23654327
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG678150.2866917329755355Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG591070.2498781723952662Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT337110.14251515166759976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32400.026.07428643
CGATATC29050.023.62811935
ACCGATA28900.023.51964833
GACCGAT36800.023.31205232
GATATCG29750.022.62880136
CCGATAT32350.021.0128834
GAGTACT99850.019.56335420
TCAACGC132350.019.37607612
ATGCCGT43800.018.93634644
CAACGCA134800.018.90970413
GAGACCG47600.018.76160430
TCGTATG45350.018.57988740
AGACCGA48000.018.5600231
TGCCGAG222850.018.15081626
ATCGTAT37300.018.1072339
GCCGAGA223700.018.05196827
AAGCAGT157850.017.9641821
ATGCCGA225350.017.91230425
CGTATGC48550.017.76203541
GGTATCA149450.017.4386448
Produced by FastQC (version 0.11.5)