FastQCFastQC Report
Fri 27 May 2016
SRR522043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26238878
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1046310.39876323980011646Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG704470.26848327889630036Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT564150.21500538247100354No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG273830.10436040748388709Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33600.021.4111643
AAGCAGT202000.020.2316041
TGCCGAG303400.019.6918426
GCCGAGA313300.019.07530827
ATGCCGA314650.019.0521525
ACCGATA31800.018.81441533
GACCGAT40100.018.54035232
GATATCG32850.018.07915536
CGAGATC271200.018.00510629
CCGATAT33500.017.99100934
AGATCGG275050.017.82539231
GAGATCG277750.017.59674530
CGATATC34150.017.58416435
CCGAGAT280500.017.51832628
AATGCCG351100.017.03662524
GAGTACT179400.016.66488320
TCGTATG45300.016.55789440
ATCGTAT39250.015.9718139
GTACTTT193750.015.44195922
AGCAGTG265000.015.4115892
Produced by FastQC (version 0.11.5)