##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522043_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26238878 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.849682673169184 40.0 39.0 40.0 38.0 40.0 2 38.80322047307053 40.0 39.0 40.0 37.0 40.0 3 38.77637538464869 40.0 39.0 40.0 37.0 40.0 4 38.72725426750336 40.0 39.0 40.0 37.0 40.0 5 38.70191800884169 40.0 39.0 40.0 37.0 40.0 6 38.85098147108272 40.0 39.0 40.0 37.0 40.0 7 38.83908317268749 40.0 39.0 40.0 37.0 40.0 8 38.841264439737095 40.0 39.0 40.0 37.0 40.0 9 38.83906484111097 40.0 39.0 40.0 37.0 40.0 10 38.817348096972744 40.0 39.0 40.0 37.0 40.0 11 38.95447130018288 40.0 39.0 40.0 38.0 40.0 12 38.91985019328951 40.0 39.0 40.0 37.0 40.0 13 38.89653761109755 40.0 39.0 40.0 37.0 40.0 14 38.8721711347566 40.0 39.0 40.0 37.0 40.0 15 38.848320000573196 40.0 39.0 40.0 37.0 40.0 16 38.843200764910755 40.0 39.0 40.0 37.0 40.0 17 38.79329085641543 40.0 39.0 40.0 36.0 40.0 18 38.75883038901282 40.0 39.0 40.0 36.0 40.0 19 38.726077959583485 40.0 39.0 40.0 36.0 40.0 20 38.677950977934344 40.0 39.0 40.0 36.0 40.0 21 38.59895472664647 40.0 39.0 40.0 36.0 40.0 22 38.542109536848336 40.0 39.0 40.0 36.0 40.0 23 38.47712432673379 40.0 39.0 40.0 35.0 40.0 24 38.413934239108855 40.0 38.0 40.0 35.0 40.0 25 38.3121654058531 40.0 38.0 40.0 35.0 40.0 26 38.1554204413771 40.0 38.0 40.0 35.0 40.0 27 38.110725085119874 40.0 38.0 40.0 35.0 40.0 28 38.04324125444693 40.0 38.0 40.0 35.0 40.0 29 37.98092075430969 40.0 38.0 40.0 35.0 40.0 30 37.902932815953484 40.0 38.0 40.0 35.0 40.0 31 37.73106090893064 40.0 38.0 40.0 34.0 40.0 32 37.64486057673655 39.0 38.0 40.0 33.0 40.0 33 37.781720011046204 40.0 38.0 40.0 34.0 40.0 34 37.725078831495765 40.0 38.0 40.0 34.0 40.0 35 37.64564144091832 40.0 38.0 40.0 33.0 40.0 36 37.662269629059594 40.0 38.0 40.0 34.0 40.0 37 37.56996884546664 40.0 38.0 40.0 33.0 40.0 38 37.79226341156813 40.0 38.0 40.0 35.0 40.0 39 37.74368797324337 40.0 38.0 40.0 34.0 40.0 40 37.69232712618276 40.0 38.0 40.0 34.0 40.0 41 37.775843273481435 40.0 38.0 40.0 35.0 40.0 42 37.71164315791247 40.0 38.0 40.0 35.0 40.0 43 37.636607861052596 40.0 38.0 40.0 34.0 40.0 44 37.578843729522276 40.0 38.0 40.0 34.0 40.0 45 37.49460361834069 40.0 38.0 40.0 34.0 40.0 46 37.32444977258555 40.0 38.0 40.0 33.0 40.0 47 37.242782561053104 40.0 38.0 40.0 33.0 40.0 48 37.142909007008605 40.0 38.0 40.0 33.0 40.0 49 37.03038434036699 40.0 38.0 40.0 33.0 40.0 50 36.854416450276574 40.0 38.0 40.0 33.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 -0.15628089896658537 1 2 -0.1796067901688616 1 3 -0.13487112514244615 1 4 -0.1027720312257685 1 5 -0.10130377461422313 1 6 -0.15485175681224206 1 7 -0.15433713694398676 1 8 -0.15885553703430588 1 9 -0.17874970504012566 1 10 -0.18922439518262735 1 11 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-0.4496214823197988 120 22 -0.48052428200214337 120 23 -0.48791627532401094 120 24 -0.5139992457602318 120 25 -0.5081029848581267 120 26 -0.5818236404817938 120 27 -0.64279391437627 120 28 -0.7256469167919448 120 29 -0.7433525094650193 120 30 -0.7699126904267501 120 31 -0.885093530759022 120 32 -0.9082998904712625 120 33 -0.8425603979550971 120 34 -0.8459369157836889 120 35 -0.9061373310557528 120 36 -0.8839848318665773 120 37 -0.9163006271741239 120 38 -0.8862014933565163 120 39 -0.9063381533636132 120 40 -0.9122963492058744 120 41 -0.8709846205653662 120 42 -0.8842941650859899 120 43 -0.9246856837575805 120 44 -0.9310451241890263 120 45 -0.945212538176591 120 46 -0.9957537504569842 120 47 -1.0068236208877863 120 48 -1.0065781627895305 120 49 -1.0174581744280715 120 50 -0.9472144622622807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16227.0 3 656.0 4 755.0 5 818.0 6 1630.0 7 2642.0 8 3464.0 9 4076.0 10 5763.0 11 9182.0 12 9554.0 13 12370.0 14 17114.0 15 21423.0 16 21979.0 17 23562.0 18 29359.0 19 35405.0 20 39278.0 21 35903.0 22 46137.0 23 56768.0 24 64177.0 25 70043.0 26 91855.0 27 118331.0 28 147006.0 29 163999.0 30 169695.0 31 206030.0 32 251514.0 33 320110.0 34 440593.0 35 640481.0 36 1016717.0 37 1832728.0 38 3589538.0 39 1.6217913E7 40 504083.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.199020999308075 26.984334111099678 23.72828496101875 28.088359928573496 2 25.054463075745847 28.714726292793465 25.36515852545219 20.865652106008497 3 23.672060215379638 25.922190727819995 26.107579752457404 24.298169304342967 4 25.38762814909224 25.02045533895113 24.72571323667089 24.866203275285738 5 26.296684874629683 27.09887875273515 25.402253247338823 21.202183125296344 6 26.2608370678045 25.99399638963221 22.94770378519996 24.797462757363327 7 24.725664717828256 26.37087988289743 25.578890987640552 23.324564411633762 8 24.965023275766594 26.081431530723226 25.210818846750993 23.742726346759188 9 24.343944889716703 25.505183567681517 25.66289229287929 24.487979249722493 10 24.227888097959067 27.053424311816993 24.452886285762677 24.265801304461267 11 24.948353355657968 26.23683832822425 24.711201446952114 24.10360686916567 12 24.14901277409804 25.798759382927884 25.568208366226635 24.48401947674744 13 24.556995158100893 25.952317778222074 25.174571107804226 24.316115955872807 14 24.40589875832343 26.6687737181445 25.12833056352486 23.796996960007206 15 23.679217533615578 26.044436808616588 26.13987915184483 24.136466505923003 16 24.298645696664316 25.598232515887304 25.776540445060185 24.32658134238819 17 24.220326799034623 25.821153633169835 25.344220130144286 24.614299437651262 18 23.74672423111994 26.37349051281842 25.36220108192126 24.51758417414037 19 25.083469651408112 25.88424322107066 25.172528337530288 23.859758789990945 20 24.419752247028246 25.872550647935476 25.439262303822595 24.26843480121368 21 23.99908639386181 26.440254038301486 25.535550719813553 24.02510884802315 22 24.671653262003048 25.77653663392162 25.381546421306584 24.170263682768752 23 24.37619855544128 25.678472227356675 26.12865534875386 23.816673868448184 24 23.855162556874575 26.267319814513414 25.564046602907336 24.31347102570468 25 24.37211193063857 25.830904185732408 25.353001026678506 24.443982856950516 26 24.414146684253193 25.56341784003773 26.250348958172133 23.77208651753695 27 24.63923084698028 25.63574705920107 25.825593064865803 23.899429028952852 28 24.304913401977142 25.685584990677146 25.465618778743504 24.54388282860221 29 24.528758169959552 25.599922492411697 25.642266577089806 24.22905276053894 30 24.3974672774475 25.82909443232011 25.756890893115663 24.01654739711672 31 24.359417973086284 25.872899546668986 25.6272561599787 24.14042632026603 32 24.562172646613423 25.56184175352452 25.629724001929716 24.246261597932346 33 24.222088789095647 25.961790401542455 25.703530606228703 24.1125902031332 34 24.464738317230555 25.35680999640444 25.91221619218662 24.266235494178385 35 24.060639851531327 25.703578013760424 25.89636714972826 24.33941498497999 36 24.37327220731721 25.600805899870068 25.97237589908109 24.053545993731635 37 24.65242128344743 25.458233879117287 25.428650116783825 24.460694720651453 38 24.098974437118915 26.413654545306247 25.643863023395387 23.843507994179454 39 24.311829868043322 25.959829501534053 25.636866075888992 24.091474554533633 40 24.5609206568565 25.413136634508227 25.45511987635316 24.57082283228211 41 24.158930025156717 26.304673879125712 25.515844600902803 24.020551494814768 42 24.769858054200093 25.473572562699925 25.62159841083273 24.13497097226725 43 24.430052034650735 25.395309975190887 25.520003482087905 24.65463450807047 44 24.552662990810664 25.954011627938 25.57782372280164 23.915501658449692 45 24.35224945276206 25.787438151183228 25.611547523838595 24.248764872216114 46 24.21087412669107 25.575282651574383 26.002462163362583 24.211381058371963 47 24.50451835654767 25.678264229026826 25.832040965863506 23.985176448561994 48 23.942853610134158 25.529313580271758 25.49937418857769 25.02845862101639 49 24.283830263753543 25.989244001810775 25.194672511972556 24.53225322246313 50 24.937778936716903 25.714705782209368 25.378850256553815 23.968665024519915 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8306.0 1 5577.0 2 2848.0 3 2681.0 4 2514.0 5 2402.5 6 2291.0 7 2486.5 8 2682.0 9 3353.5 10 4025.0 11 6040.0 12 8055.0 13 12168.5 14 16282.0 15 21689.5 16 27097.0 17 39596.0 18 52095.0 19 69818.0 20 87541.0 21 114260.5 22 140980.0 23 208272.5 24 275565.0 25 290569.5 26 305574.0 27 357139.5 28 408705.0 29 458215.5 30 507726.0 31 576241.0 32 644756.0 33 726997.0 34 809238.0 35 896336.5 36 983435.0 37 1070832.0 38 1158229.0 39 1241351.0 40 1324473.0 41 1386837.5 42 1449202.0 43 1492610.0 44 1536018.0 45 1591059.5 46 1646101.0 47 1691153.5 48 1736206.0 49 1747779.5 50 1759353.0 51 1745336.0 52 1731319.0 53 1694589.0 54 1657859.0 55 1612039.0 56 1566219.0 57 1534133.0 58 1502047.0 59 1409103.5 60 1316160.0 61 1149279.0 62 982398.0 63 859720.5 64 737043.0 65 640712.5 66 544382.0 67 476983.0 68 409584.0 69 355180.0 70 300776.0 71 253257.5 72 205739.0 73 175009.0 74 144279.0 75 119317.0 76 94355.0 77 77656.0 78 60957.0 79 49724.0 80 38491.0 81 30236.0 82 21981.0 83 17226.5 84 12472.0 85 9483.0 86 6494.0 87 4822.0 88 3150.0 89 2522.0 90 1894.0 91 1607.0 92 1320.0 93 835.5 94 351.0 95 260.0 96 169.0 97 116.5 98 64.0 99 71.0 100 78.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5523216350943055 2 0.0 3 0.0 4 2.6677969995515817E-5 5 0.050204128392989975 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.02187212425775218 26 0.013880166674809801 27 0.0029345766995067394 28 0.004619099947795024 29 0.005514717511930198 30 0.0021037484910749614 31 0.0016578452782927684 32 0.0030336663023472266 33 6.974383584541991E-4 34 0.0036739375822396067 35 0.0033728576351473565 36 0.009024776135625922 37 0.005762441519031416 38 0.008247303867185174 39 0.00823587045147281 40 0.008456916488578513 41 0.008190136788623355 42 0.008552194952848213 43 0.006654247944595802 44 0.006570402896038466 45 0.008075802631499715 46 0.00991277142261952 47 0.010633076612498446 48 0.009680291969801452 49 0.02668940341122818 50 0.045074335876709365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2.6238878E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.136798422737876 #Duplication Level Percentage of deduplicated Percentage of total 1 73.2317512030947 37.44837299416745 2 14.761033648104984 15.09664004348791 3 5.1643606470906 7.922666081777763 4 2.218564100598513 4.538010608009156 5 1.2207201996010542 3.1211861388781723 6 0.7303928622924807 2.2409971541074274 7 0.5139111363836183 1.8395839131914469 8 0.3565322486278469 1.458553418343013 9 0.26233758650738287 1.2073593851942037 >10 1.3858032990245053 12.517072906671206 >50 0.07776122525550846 2.8078841308392795 >100 0.07337105020083122 7.301813431333979 >500 0.002845504131624598 0.9578388395066424 >1k 5.407843907455298E-4 0.3664779911455993 >5k 3.725234773335992E-5 0.14045312819659636 >10k+ 3.725234773083587E-5 1.0350898351502147 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG 104631 0.39876323980011646 Illumina Paired End PCR Primer 2 (97% over 36bp) CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG 70447 0.26848327889630036 Illumina Paired End PCR Primer 2 (100% over 31bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 56415 0.21500538247100354 No Hit CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG 27383 0.10436040748388709 Illumina Paired End PCR Primer 2 (96% over 33bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.591574228135822E-4 6.097821713260757E-5 0.0 0.0 1.143341571236392E-5 2 3.0870222423382584E-4 1.143341571236392E-4 0.0 3.8111385707879733E-6 1.9055692853939867E-5 3 3.3919133280012966E-4 1.9817920568097463E-4 0.0 7.622277141575947E-6 3.0489108566303787E-5 4 4.8020345991928463E-4 2.3629059138885437E-4 3.8111385707879733E-6 1.143341571236392E-5 4.192252427866771E-5 5 0.0011890752340858477 3.0870222423382584E-4 3.8111385707879733E-6 1.143341571236392E-5 6.478935570339555E-5 6 0.0012157532040813635 3.734915799372214E-4 7.622277141575947E-6 1.5244554283151893E-5 8.003390998654744E-5 7 0.001291975975497123 4.1922524278667706E-4 7.622277141575947E-6 1.5244554283151893E-5 8.384504855733542E-5 8 0.0015015885968904616 4.573366284945568E-4 7.622277141575947E-6 1.5244554283151893E-5 1.0290074141127529E-4 9 0.001718823495425376 5.183148456271644E-4 7.622277141575947E-6 1.9055692853939867E-5 1.2195643426521515E-4 10 0.002694474969547097 5.67859647047408E-4 1.143341571236392E-5 1.9055692853939867E-5 1.2195643426521515E-4 11 0.0030832111037674705 6.135933098968638E-4 1.143341571236392E-5 1.9055692853939867E-5 1.2195643426521515E-4 12 0.003624392780819363 6.478935570339555E-4 1.143341571236392E-5 1.9055692853939867E-5 1.3338984997757908E-4 13 0.004085540547884708 6.898160813126232E-4 1.143341571236392E-5 1.9055692853939867E-5 1.3720098854836705E-4 14 0.004600044254941084 7.16494051308139E-4 1.143341571236392E-5 1.9055692853939867E-5 1.4863440426073096E-4 15 0.017462636931350493 7.507942984452308E-4 1.143341571236392E-5 1.9055692853939867E-5 1.5244554283151893E-4 16 0.018327765386919364 8.155836541486264E-4 1.143341571236392E-5 1.9055692853939867E-5 1.7150123568545882E-4 17 0.02005040002091553 8.61317316998082E-4 1.143341571236392E-5 2.286683142472784E-5 1.867457899686107E-4 18 0.021662511636358843 9.108621184183256E-4 1.143341571236392E-5 2.286683142472784E-5 1.9055692853939867E-4 19 0.024208352201645206 9.642180584093573E-4 1.143341571236392E-5 2.286683142472784E-5 2.0580148282255058E-4 20 0.30751314899974 0.001021385136971177 1.143341571236392E-5 2.286683142472784E-5 2.2485717567649044E-4 21 0.31656460310536144 0.0010747410769622085 1.143341571236392E-5 2.6677969995515813E-5 2.324794528180664E-4 22 0.31839013848076886 0.0010976079083869364 1.5244554283151893E-5 2.6677969995515813E-5 2.4772400710121827E-4 23 0.32011658425333583 0.001124285878382452 1.5244554283151893E-5 3.0489108566303787E-5 2.629685613843702E-4 24 0.3221479211115658 0.0011700195412319078 1.5244554283151893E-5 3.0489108566303787E-5 2.7059083852594613E-4 25 0.3236037760456068 0.0012005086497982116 1.5244554283151893E-5 3.430024713709176E-5 2.8583539280909804E-4 26 0.32518920969105464 0.0012386200355060913 1.5244554283151893E-5 3.430024713709176E-5 2.972688085214619E-4 27 0.32723960224213855 0.0012652980055016072 1.5244554283151893E-5 3.430024713709176E-5 3.125133628046138E-4 28 0.4384333811834485 0.001311031668351063 1.5244554283151893E-5 3.430024713709176E-5 3.315690556585537E-4 29 0.4422597643085196 0.0013872544397668224 1.5244554283151893E-5 3.811138570787973E-5 3.3919133280012966E-4 30 0.4554424926248752 0.0014482326568994299 1.5244554283151893E-5 4.192252427866771E-5 3.544358870832815E-4 31 0.8714663790120903 0.0014901551811780977 1.5244554283151893E-5 4.192252427866771E-5 3.544358870832815E-4 32 0.8770230190482993 0.0015663779525938572 1.5244554283151893E-5 4.573366284945568E-5 3.6586930279564544E-4 33 0.9063344857962295 0.0016083004768725248 1.5244554283151893E-5 5.335593999103163E-5 3.7730271850800937E-4 34 0.909436752592851 0.0016654675554343445 1.5244554283151893E-5 5.335593999103163E-5 3.925472727911613E-4 35 0.9123484624609329 0.0017112012182838002 1.5244554283151893E-5 5.335593999103163E-5 4.077918270743132E-4 36 0.914970525797635 0.0017378791882793159 1.5244554283151893E-5 5.335593999103163E-5 4.2303638135746504E-4 37 0.917596400272908 0.0017569348811332557 1.5244554283151893E-5 5.335593999103163E-5 4.2303638135746504E-4 38 0.9205385992495563 0.0017836128511287716 1.5244554283151893E-5 5.71670785618196E-5 4.3828093564061694E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 3360 0.0 21.41116 43 AAGCAGT 20200 0.0 20.231604 1 TGCCGAG 30340 0.0 19.69184 26 GCCGAGA 31330 0.0 19.075308 27 ATGCCGA 31465 0.0 19.05215 25 ACCGATA 3180 0.0 18.814415 33 GACCGAT 4010 0.0 18.540352 32 GATATCG 3285 0.0 18.079155 36 CGAGATC 27120 0.0 18.005106 29 CCGATAT 3350 0.0 17.991009 34 AGATCGG 27505 0.0 17.825392 31 GAGATCG 27775 0.0 17.596745 30 CGATATC 3415 0.0 17.584164 35 CCGAGAT 28050 0.0 17.518326 28 AATGCCG 35110 0.0 17.036625 24 GAGTACT 17940 0.0 16.664883 20 TCGTATG 4530 0.0 16.557894 40 ATCGTAT 3925 0.0 15.97181 39 GTACTTT 19375 0.0 15.441959 22 AGCAGTG 26500 0.0 15.411589 2 >>END_MODULE