FastQCFastQC Report
Fri 27 May 2016
SRR522042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26524622
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1571730.5925550984289238Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG606640.22870825454176122Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT586120.2209720462745897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32350.025.84598543
TGCCGAG337100.024.69863526
CGAGATC295300.024.4252929
ATGCCGA344050.024.2903225
GCCGAGA344800.024.21660827
GAGATCG299750.024.0919430
AGATCGG300000.024.0426731
ACCGATA31900.023.78976633
CCGAGAT313700.023.10495428
GATATCG33150.022.9594636
CGATATC33950.022.41827435
GACCGAT37950.022.37388432
AATGCCG377150.022.04761124
CCGATAT35250.021.4042134
AAGCAGT211950.020.0854931
GAATGCC431050.019.4487223
GAGTACT156300.019.22450320
GGAATGC443350.019.19124422
GTTCAGC474900.018.05155614
CAGGAAT475700.017.96012920
Produced by FastQC (version 0.11.5)