FastQCFastQC Report
Fri 27 May 2016
SRR522041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29038282
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1158550.39897332769204463Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG610870.21036712846855057Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36150.024.70923643
ACCGATA31950.023.1338933
GACCGAT39700.023.10549732
GATATCG32350.022.91659536
CGATATC33650.022.0965235
TGCCGAG294850.021.57509426
GCCGAGA295650.021.46481927
AGATCGG246700.021.38166831
CGAGATC247300.021.37448729
GAGATCG250200.021.15278830
ATGCCGA302150.021.08339325
CCGATAT37350.020.02533534
CCGAGAT270300.019.66961728
AATGCCG330600.019.18228724
GAGACCG52600.017.85670930
TCGTATG52000.017.76865840
AGACCGA53300.017.74609631
CGAGACC55600.016.93304329
ATCGTAT44400.016.89532739
GATCGGA386950.016.8481521
Produced by FastQC (version 0.11.5)