##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522041_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29038282
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.92995353512994	40.0	39.0	40.0	38.0	40.0
2	38.90867448700994	40.0	39.0	40.0	38.0	40.0
3	38.902756230551105	40.0	39.0	40.0	38.0	40.0
4	38.87889703667731	40.0	39.0	40.0	38.0	40.0
5	38.84697166313076	40.0	39.0	40.0	38.0	40.0
6	38.969604090214425	40.0	39.0	40.0	38.0	40.0
7	38.94962704749544	40.0	39.0	40.0	38.0	40.0
8	38.952076985821684	40.0	39.0	40.0	38.0	40.0
9	38.947793881194485	40.0	39.0	40.0	38.0	40.0
10	38.93086794873057	40.0	39.0	40.0	38.0	40.0
11	39.025133614998296	40.0	39.0	40.0	38.0	40.0
12	38.98963926309415	40.0	39.0	40.0	38.0	40.0
13	38.96663290893036	40.0	39.0	40.0	38.0	40.0
14	38.948308581065504	40.0	39.0	40.0	38.0	40.0
15	38.92725437407075	40.0	39.0	40.0	37.0	40.0
16	38.92789098198027	40.0	39.0	40.0	37.0	40.0
17	38.88376385352274	40.0	39.0	40.0	37.0	40.0
18	38.851271400973374	40.0	39.0	40.0	37.0	40.0
19	38.827759954944995	40.0	39.0	40.0	37.0	40.0
20	38.7890879012746	40.0	39.0	40.0	36.0	40.0
21	38.73337578993137	40.0	39.0	40.0	36.0	40.0
22	38.679774375081834	40.0	39.0	40.0	36.0	40.0
23	38.63169439569462	40.0	39.0	40.0	36.0	40.0
24	38.57494895875727	40.0	39.0	40.0	36.0	40.0
25	38.48225990091287	40.0	39.0	40.0	35.0	40.0
26	38.34364632866366	40.0	38.0	40.0	35.0	40.0
27	38.30470173820889	40.0	38.0	40.0	35.0	40.0
28	38.23629014967208	40.0	38.0	40.0	35.0	40.0
29	38.17832907607964	40.0	38.0	40.0	35.0	40.0
30	38.13516081977577	40.0	38.0	40.0	35.0	40.0
31	38.02421334705683	40.0	38.0	40.0	35.0	40.0
32	37.947900499072226	40.0	38.0	40.0	35.0	40.0
33	38.058496814653154	40.0	38.0	40.0	35.0	40.0
34	38.01261272963738	40.0	38.0	40.0	35.0	40.0
35	37.950164475983804	40.0	38.0	40.0	35.0	40.0
36	37.975699629888574	40.0	38.0	40.0	35.0	40.0
37	37.881436856353965	40.0	38.0	40.0	35.0	40.0
38	38.09839755671496	40.0	38.0	40.0	35.0	40.0
39	38.05933983973294	40.0	38.0	40.0	35.0	40.0
40	38.01766760857271	40.0	38.0	40.0	35.0	40.0
41	38.09819909456076	40.0	39.0	40.0	35.0	40.0
42	38.0378360882369	40.0	38.0	40.0	35.0	40.0
43	37.97757446532133	40.0	38.0	40.0	35.0	40.0
44	37.93341007570627	40.0	38.0	40.0	35.0	40.0
45	37.87369745221153	40.0	38.0	40.0	35.0	40.0
46	37.75679976522027	40.0	38.0	40.0	35.0	40.0
47	37.692751899027634	40.0	38.0	40.0	34.0	40.0
48	37.61084140583799	40.0	38.0	40.0	34.0	40.0
49	37.529602612165554	40.0	38.0	40.0	33.0	40.0
50	37.421920759637224	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.30061264386662856
1	2	-0.38526264211043326
1	3	-0.2895304438513904
1	4	-0.3877492269013487
1	5	-0.32641157909871055
1	6	-0.31548404952942377
1	7	-0.3477301681178986
1	8	-0.3032682850893238
1	9	-0.3272095295737927
1	10	-0.2987810660498553
1	11	-0.27478722829973634
1	12	-0.3209650447331853
1	13	-0.28866949246781104
1	14	-0.3075345988127296
1	15	-0.29402263885563684
1	16	-0.2698252319211605
1	17	-0.33250952908213094
1	18	-0.28273085968205436
1	19	-0.26735861163372476
1	20	-0.31697512156002006
1	21	-0.30877370933227155
1	22	-0.33959358231936676
1	23	-0.3263836695391191
1	24	-0.27617234980428407
1	25	-0.3351571868535643
1	26	-0.5168480411422394
1	27	-0.5345829742410402
1	28	-0.3814676806228263
1	29	-0.6136204262904172
1	30	-0.42037798367284296
1	31	-0.35860149817332143
1	32	-0.3828813263691302
1	33	-0.2998512013483392
1	34	-0.2446177741731006
1	35	-0.2673407071979099
1	36	-0.15447248820255055
1	37	-0.14139726413559828
1	38	-0.21541990770941766
1	39	-0.14931288034781431
1	40	-0.11621485025879252
1	41	-0.12270417560412028
1	42	-0.14034737857770097
1	43	-0.15975733495297817
1	44	-0.11398441781869906
1	45	-0.1341344858643012
1	46	-0.10637441336352538
1	47	-0.1492748325871247
1	48	-0.0723701291952068
1	49	-0.15515540622425306
1	50	-0.19344140731585213
2	1	-0.21339819213121558
2	2	-0.2488798161116037
2	3	-0.2507668848454685
2	4	-0.285089870841027
2	5	-0.2698386394625274
2	6	-0.25716051136463136
2	7	-0.27616236340550415
2	8	-0.23722597714939297
2	9	-0.26920595658266677
2	10	-0.24616548886750422
2	11	-0.24160703806837347
2	12	-0.2636401795095651
2	13	-0.2736634176370387
2	14	-0.2743695802363604
2	15	-0.25814700809121405
2	16	-0.21507596248287086
2	17	-0.2349902961168695
2	18	-0.26177638169756534
2	19	-0.22768813671601862
2	20	-0.23341178821459607
2	21	-0.2278299862571771
2	22	-0.24016645497471245
2	23	-0.2542120884954713
2	24	-0.29250221943185295
2	25	-0.3052102325594035
2	26	-0.34249328720170524
2	27	-0.39668722111221655
2	28	-0.5897143126734505
2	29	-0.3963771976998274
2	30	-0.4410523383677116
2	31	-0.36268285239945897
2	32	-0.39921227142187377
2	33	-0.32586686281016597
2	34	-0.3364807323319141
2	35	-0.35709287856079897
2	36	-0.26193951482948563
2	37	-0.26652393518417483
2	38	-0.30287636664594686
2	39	-0.30433048736582435
2	40	-0.25406044326795296
2	41	-0.26584381391505474
2	42	-0.3063828358278826
2	43	-0.2263630463260924
2	44	-0.2228022624969057
2	45	-0.25569347714467483
2	46	-0.6918333638251113
2	47	-0.7576019088763672
2	48	-0.8139752144650387
2	49	-1.1814928134617873
2	50	-1.2045157118888525
3	1	-0.19283448929279956
3	2	-0.303106628230168
3	3	-0.24032867567043326
3	4	-0.28157198719078025
3	5	-0.20820984780517193
3	6	-0.24734093494395637
3	7	-0.2803429525199377
3	8	-0.247875308116015
3	9	-0.2304895937461282
3	10	-0.23304200476756876
3	11	-0.22404557838168415
3	12	-0.2030074976797991
3	13	-0.22780629160780563
3	14	-0.20451521364089587
3	15	-0.20056026545395156
3	16	-0.1880932004409459
3	17	-0.21533014822828278
3	18	-0.1999829188337685
3	19	-0.21551200567184736
3	20	-0.1989828402672913
3	21	-0.18915766332381168
3	22	-0.2138538054117305
3	23	-0.20568024278037456
3	24	-0.2711370146569223
3	25	-0.2738536453163718
3	26	-0.3182455933039421
3	27	-0.30478859805560177
3	28	-0.38236583010430536
3	29	-0.2908271086653471
3	30	-0.28888774267977624
3	31	-0.26548427456476276
3	32	-0.26477755873690256
3	33	-0.22489717765351713
3	34	-0.18707392765278286
3	35	-0.23833945887659524
3	36	-0.18097306960996207
3	37	-0.16613159013270717
3	38	-0.17203296013099134
3	39	-0.15664263015582236
3	40	-0.1844859794490077
3	41	-0.28867855292020295
3	42	-0.2756699845092925
3	43	-0.29647381883926016
3	44	-0.36603345647466057
3	45	-0.7032147338736223
3	46	-1.134060318241957
3	47	-0.9061825640969587
3	48	-0.9545152611257848
3	49	-0.7957675201931522
3	50	-0.8760571966890609
4	1	-0.15580113194094736
4	2	-0.19816393753589523
4	3	-0.1796345331991418
4	4	-0.136815158415871
4	5	-0.11898534885012424
4	6	-0.1570031425221714
4	7	-0.18807055414001184
4	8	-0.17770074280235093
4	9	-0.18349399269254718
4	10	-0.15848973222846752
4	11	-0.1683303432337695
4	12	-0.1662454064917469
4	13	-0.156565890921172
4	14	-0.16218737097660352
4	15	-0.16361663807774818
4	16	-0.13892548019358486
4	17	-0.15312703711339282
4	18	-0.14815342915549223
4	19	-0.17252986055535047
4	20	-0.16335733794844032
4	21	-0.1739055648559571
4	22	-0.15238945541089066
4	23	-0.187182122583188
4	24	-0.2092646321780407
4	25	-0.26184413446458166
4	26	-0.21945707763049427
4	27	-0.1188199984109275
4	28	-0.19795819888688015
4	29	-0.15187057854966213
4	30	-0.10854739437163374
4	31	-0.08677456688496932
4	32	-0.12562875332011458
4	33	-0.041235337220058454
4	34	-0.06421055840215217
4	35	-0.021878367566408485
4	36	-0.09223036147830044
4	37	-0.08750906362568855
4	38	-0.10418477878444321
4	39	-0.12462715055486484
4	40	-0.35774309869557896
4	41	-0.4819723527015327
4	42	-0.650089409427828
4	43	-0.646437919465157
4	44	-0.47878993251707413
4	45	-0.23396879759163625
4	46	-0.22094874162161204
4	47	-0.22801555269111873
4	48	-0.24836534270336585
4	49	-0.17158321626990158
4	50	-0.20624740654124452
5	1	-0.12890241178860862
5	2	-0.1865306875115209
5	3	-0.15433605512460957
5	4	-0.22309413359915453
5	5	-0.12856873631258026
5	6	-0.1389954166700207
5	7	-0.12575847898692416
5	8	-0.1425689299994275
5	9	-0.15120587306738997
5	10	-0.14412816148726648
5	11	-0.1311091206478423
5	12	-0.1257374631785666
5	13	-0.1393499131588527
5	14	-0.13161483992354306
5	15	-0.11564473843748146
5	16	-0.1399523875372637
5	17	-0.11491671463873843
5	18	-0.13285072933071973
5	19	-0.13469132090890668
5	20	-0.15518846214297355
5	21	-0.15483016919942116
5	22	-0.1620705346364275
5	23	-0.15506125420624528
5	24	-0.128179395098158
5	25	-0.20189734277074223
5	26	-0.13934241193006613
5	27	-0.11377398982178022
5	28	-0.1375382050878997
5	29	-0.16642325351168807
5	30	-0.0934417866791577
5	31	-0.1106341311350647
5	32	-0.07931334343524554
5	33	-0.01762840129339338
5	34	-0.019992958514357895
5	35	-0.03741310285600008
5	36	-0.11322930618420912
5	37	-0.051832719604718136
5	38	-0.13584495059904356
5	39	-0.40129961964018435
5	40	-0.264569082394047
5	41	-0.26793149538202243
5	42	-0.22550045316225464
5	43	-0.11955897876585908
5	44	-0.10685650677645242
5	45	-0.09540570468671916
5	46	0.006453598758781709
5	47	-0.02799644564470327
5	48	0.009527161190597155
5	49	0.023569827236649132
5	50	0.05511850796882811
6	1	-0.07691158854728286
6	2	-0.12564529915647427
6	3	-0.08463412169835038
6	4	-0.06801228530312642
6	5	-0.13739892325815362
6	6	-0.06569307169980476
6	7	-0.0865340725807755
6	8	-0.05955353025026966
6	9	-0.08050171848168475
6	10	-0.05921937605044292
6	11	-0.0860291344482178
6	12	-0.06926200347971445
6	13	-0.09638915251593971
6	14	-0.10260338327013585
6	15	-0.08144127665999434
6	16	-0.0689558720150032
6	17	-0.07737448825695026
6	18	-0.08287578301584375
6	19	-0.07396234141069158
6	20	-0.06865049790487632
6	21	-0.07963568968365564
6	22	-0.09034590535802067
6	23	-0.06635526584580731
6	24	-0.07724400611552085
6	25	-0.09021063744713587
6	26	-0.06314226625582364
6	27	-0.06548496220675304
6	28	-0.060466492089013
6	29	-0.05387155841123814
6	30	-0.08160697858048849
6	31	-0.012100987320934564
6	32	0.002696540346128984
6	33	0.054554081633412466
6	34	0.05871246046243073
6	35	0.0696255746269685
6	36	0.10700581091035843
6	37	0.09398945402366365
6	38	0.12340726549146552
6	39	0.1250550065496654
6	40	0.09477857183650684
6	41	0.10604666748111669
6	42	0.11254179692820543
6	43	0.12057702614880839
6	44	0.1255140103397565
6	45	0.13112816336839472
6	46	0.14984642718533792
6	47	0.14557964600294326
6	48	0.18253039737093246
6	49	0.15194815636088777
6	50	0.1893658133600411
7	1	-0.05958030020372718
7	2	-0.11046205211413707
7	3	-0.048926078949342866
7	4	-0.026242690500659194
7	5	-0.015939137003179837
7	6	-0.05511615178674134
7	7	-0.06424407476890082
7	8	-0.05649992700460871
7	9	-0.07327198864079776
7	10	-0.07764698785446456
7	11	-0.0722270575414683
7	12	-0.06107338648836702
7	13	-0.0671969646072128
7	14	-0.06942190235533019
7	15	-0.06687082733806449
7	16	-0.03992904777060602
7	17	-0.03901032378252012
7	18	-0.05850817924042673
7	19	-0.040416858896747954
7	20	-0.03924552329861797
7	21	-0.06326156102534242
7	22	-0.07139020332438406
7	23	-0.06492733075901924
7	24	-0.06857552396517264
7	25	-0.07104157289422375
7	26	-0.05771286364619499
7	27	-0.058167995372443215
7	28	-0.10769730365217356
7	29	0.011179496411926948
7	30	-0.015171401516617777
7	31	8.829291553027474E-4
7	32	-0.023803824889377267
7	33	-0.005994540754208799
7	34	0.040724406295701954
7	35	0.04528359491892786
7	36	0.02090746384779152
7	37	0.070878581193071
7	38	0.07293414567283207
7	39	0.054442644808574414
7	40	0.0582161124525058
7	41	0.06346318978104648
7	42	0.06469300524899779
7	43	0.09212158158975114
7	44	0.10307205728403801
7	45	0.12254644521503621
7	46	0.12246707006562474
7	47	0.11487076419940223
7	48	0.1474119755173433
7	49	0.12924480080083356
7	50	0.12532065741928733
8	1	-0.04780615743650429
8	2	-0.06155861584151978
8	3	-0.058635027525127725
8	4	-0.04950453522505427
8	5	-0.0353599401646747
8	6	-0.08613314284441032
8	7	-0.08255923292199441
8	8	-0.10478478082733744
8	9	-0.0635652425146418
8	10	-0.06264140793303596
8	11	-0.10179329196974862
8	12	-0.0901005407804405
8	13	-0.0947794801855224
8	14	-0.08276756940658458
8	15	-0.10207632697714786
8	16	-0.07299754418347959
8	17	-0.08620274729462096
8	18	-0.06949890761575972
8	19	-0.06067239046753059
8	20	-0.08087925454883305
8	21	-0.06355769873167105
8	22	-0.07368977486380857
8	23	-0.06080985117813498
8	24	-0.06028218459346135
8	25	-0.06650750914294434
8	26	-0.129862115704249
8	27	-0.12706491759438876
8	28	-0.051730733778583726
8	29	-0.10846608661740476
8	30	-0.06004700096720228
8	31	0.007696115312995744
8	32	0.012416835785863611
8	33	0.047786526116979644
8	34	0.052591036652536616
8	35	0.06013755759612138
8	36	0.006185663443105227
8	37	-0.2338732727074344
8	38	-0.026171759194042465
8	39	0.020404672894549947
8	40	-0.0037887789739912137
8	41	0.04115353744613515
8	42	0.0011940882347758475
8	43	0.03195735685087442
8	44	0.036273197755278375
8	45	0.06968739411446023
8	46	0.10469821118919498
8	47	0.13217336143048186
8	48	0.10256542087124387
8	49	0.1295997928555792
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113	22	-0.0814465913380289
113	23	-0.06684009962641824
113	24	-0.06855657072066634
113	25	-0.048199802710449546
113	26	-0.02574064026924816
113	27	-0.0261471207367876
113	28	-0.013101149388781153
113	29	-0.05674103589640822
113	30	-0.03281470321432067
113	31	-0.04942521694736257
113	32	-0.04861575570485854
113	33	-0.004960120917623101
113	34	-0.019545810586997447
113	35	-0.04221508582420341
113	36	-0.042805190609065846
113	37	-0.04351266761004524
113	38	-0.0554599814308574
113	39	-0.03613462920267807
113	40	-0.05515706449671853
113	41	-0.0371200123620099
113	42	-0.0261732487377202
113	43	-0.031715202262255104
113	44	-0.03813282619830005
113	45	-0.054747116059139955
113	46	-0.025481508991006763
113	47	-0.07371912362817312
113	48	-0.04059553565936369
113	49	-0.010664226018249678
113	50	-0.03569273256378125
114	1	-0.023587465394015794
114	2	-0.019555481354025517
114	3	-0.024802470715769687
114	4	-0.01049463176491372
114	5	-0.003847881528066921
114	6	-0.029166794159500853
114	7	-0.043944354415280884
114	8	-0.034883825072505203
114	9	-0.05091148250346578
114	10	-0.03744156677918653
114	11	-0.045335533202937484
114	12	-0.04255910572452848
114	13	-0.061552149582055904
114	14	-0.0742009644376509
114	15	-0.04754836708477228
114	16	-0.06940496601739454
114	17	-0.04862863799449002
114	18	-0.05685398448076029
114	19	-0.072461848881467
114	20	-0.07534423371825483
114	21	-0.06567876128952577
114	22	-0.06618653597507063
114	23	-0.05228865363847035
114	24	-0.08367332975196717
114	25	-0.11413580594003747
114	26	-0.011088526676530819
114	27	-0.03607321609167968
114	28	-0.03253730302066771
114	29	-0.04523086837796342
114	30	-0.10515908654236483
114	31	-0.05313752929669846
114	32	-0.05714122358782703
114	33	-0.018265611584588726
114	34	-0.015142130472170834
114	35	-0.03405584845480547
114	36	-0.027221926219759496
114	37	-0.04231523526707548
114	38	-0.054234727141057704
114	39	-0.039728105935630253
114	40	-0.03705210656303137
114	41	-0.05290369552007235
114	42	-0.07197484161056167
114	43	-0.0828987772094365
114	44	-0.0551196523776909
114	45	-0.04225795925059117
114	46	-0.0646936934036475
114	47	-0.056173852019121284
114	48	-0.08040268652072058
114	49	-0.0991015371931141
114	50	-0.0617834131811108
115	1	-0.020274906333121123
115	2	-0.036485856974955766
115	3	-0.02324636591215068
115	4	-0.03701359577843277
115	5	0.008254780720967858
115	6	-0.03954639488653555
115	7	-0.05747146536280212
115	8	-0.047366363779389076
115	9	-0.06071395083667852
115	10	-0.05100169890587125
115	11	-0.060006590254481296
115	12	-0.07151636006484097
115	13	-0.06789032758674551
115	14	-0.07438919169941016
115	15	-0.07406017272896293
115	16	-0.07751757213203803
115	17	-0.08099775428770073
115	18	-0.11278079184661749
115	19	-0.10025770928832856
115	20	-0.09333436389111682
115	21	-0.07131021035719698
115	22	-0.08642049347172787
115	23	-0.06963161517892758
115	24	-0.10244201608435333
115	25	-0.05429633604589412
115	26	-0.1133324522470005
115	27	-0.12560889444947065
115	28	-0.07722864895600168
115	29	-0.09005837501436531
115	30	-0.13746604859618827
115	31	-0.1454574431562463
115	32	-0.15059633597955724
115	33	-0.11154370323635732
115	34	-0.08352351660004587
115	35	-0.11338929333290082
115	36	-0.10489685596859033
115	37	-0.06635787107139635
115	38	-0.10029884625262753
115	39	-0.10783422186964486
115	40	-0.08949309282857598
115	41	-0.13749338320383941
115	42	-0.13099606981323575
115	43	-0.15303332786188406
115	44	-0.16273193467771563
115	45	-0.15214855122928128
115	46	-0.15409210474162904
115	47	-0.16014775951801852
115	48	-0.133339572160871
115	49	-0.14714619229215486
115	50	-0.1557966807733422
116	1	-0.0420917358298567
116	2	-0.03270693610109987
116	3	-0.048750319873285264
116	4	-0.038251339422174624
116	5	-0.0416612164197403
116	6	-0.07196715673117637
116	7	-0.08785754084684072
116	8	-0.08595704632149648
116	9	-0.08953465212804446
116	10	-0.09465778488588938
116	11	-0.11589703739230828
116	12	-0.11867781607871564
116	13	-0.11852419511564705
116	14	-0.11631327655947388
116	15	-0.11890641648042077
116	16	-0.118592007950582
116	17	-0.11455917800000748
116	18	-0.1316384351470674
116	19	-0.12572945225178955
116	20	-0.13558404220356124
116	21	-0.1276824761109978
116	22	-0.13331113054481136
116	23	-0.1370958810653704
116	24	-0.15561729064191354
116	25	-0.09855361152538222
116	26	-0.08481127443860714
116	27	-0.11374241102867444
116	28	-0.14105687865057348
116	29	-0.12824159093663212
116	30	-0.1351420418081588
116	31	-0.17141344871082964
116	32	-0.2325550790566524
116	33	-0.14526648067515424
116	34	-0.15918395323431866
116	35	-0.1649952357557396
116	36	-0.14443305680300256
116	37	-0.14488012420182628
116	38	-0.17600875581081254
116	39	-0.16946118693955015
116	40	-0.185674710136972
116	41	-0.21416595537778704
116	42	-0.17857268799479442
116	43	-0.1984561942962486
116	44	-0.2205809359171269
116	45	-0.21013190892103495
116	46	-0.27783337001432784
116	47	-0.2261092226529442
116	48	-0.23274152549448957
116	49	-0.27571893013598014
116	50	-0.2750472214656696
117	1	-0.07038391485068018
117	2	-0.060440946631885595
117	3	-0.08120632345170975
117	4	-0.03782344942589333
117	5	-0.05092659521788079
117	6	-0.06999636760181005
117	7	-0.11024903854268331
117	8	-0.10398851791369168
117	9	-0.11716423084905614
117	10	-0.09669535580032829
117	11	-0.1315152300984721
117	12	-0.13851611096222172
117	13	-0.15213350157939942
117	14	-0.1475741547508278
117	15	-0.1435348153429814
117	16	-0.12092394959602615
117	17	-0.14227311451180924
117	18	-0.14503157302904413
117	19	-0.15386476985205633
117	20	-0.1835765155345186
117	21	-0.1370352254316316
117	22	-0.16660607044364895
117	23	-0.16285918456112825
117	24	-0.19729053316236644
117	25	-0.12615975035975424
117	26	-0.15046156765773588
117	27	-0.12321931695139199
117	28	-0.17463886203545087
117	29	-0.16251706815213396
117	30	-0.22567827163022258
117	31	-0.22524206549344683
117	32	-0.27368170884172827
117	33	-0.18858618889026246
117	34	-0.23412061533908712
117	35	-0.24141704949436615
117	36	-0.23124437485677163
117	37	-0.23515653588538044
117	38	-0.23557555014133413
117	39	-0.24393048861407607
117	40	-0.21710640317927243
117	41	-0.25189118240135855
117	42	-0.224534238749186
117	43	-0.23984355046982841
117	44	-0.23143826716680138
117	45	-0.26817935867654796
117	46	-0.27513509478824005
117	47	-0.2777818650377668
117	48	-0.2652210940556756
117	49	-0.2408227194272996
117	50	-0.21761614899136106
118	1	-0.1270042005553904
118	2	-0.13517303976986028
118	3	-0.12674086163814025
118	4	-0.13671051675242296
118	5	-0.16087654668773865
118	6	-0.12442851496014384
118	7	-0.1364065203769229
118	8	-0.13668783575453602
118	9	-0.155688179592687
118	10	-0.18166522377273964
118	11	-0.18189317092767254
118	12	-0.1813982357591044
118	13	-0.1850486427957776
118	14	-0.19357290063616261
118	15	-0.20949158899550469
118	16	-0.22285436746032872
118	17	-0.21219729917134345
118	18	-0.2145873546767234
118	19	-0.24229973550764328
118	20	-0.22294359565825062
118	21	-0.24498334269836874
118	22	-0.2478045740213588
118	23	-0.24590390099006498
118	24	-0.23385412054060595
118	25	-0.24211618093070797
118	26	-0.21426122621786448
118	27	-0.21295838167900172
118	28	-0.22197587297874577
118	29	-0.24781716242586072
118	30	-0.2957291345769022
118	31	-0.3454086045464848
118	32	-0.36407394176826813
118	33	-0.30924047620108297
118	34	-0.33765445504585045
118	35	-0.3247163945557645
118	36	-0.33893240486494847
118	37	-0.37218219689788157
118	38	-0.3922315455119971
118	39	-0.3939946448679521
118	40	-0.397474647857301
118	41	-0.3570903744768046
118	42	-0.3896413583562577
118	43	-0.3833604532458281
118	44	-0.3973815367994291
118	45	-0.4108419471845792
118	46	-0.4160568978780219
118	47	-0.4411793496864007
118	48	-0.4499632733428882
118	49	-0.42848558917242485
118	50	-0.41778581611027477
119	1	-0.13473007719209562
119	2	-0.16248410087950305
119	3	-0.1536600945244686
119	4	-0.129498074172254
119	5	-0.10392743107422575
119	6	-0.146355812739003
119	7	-0.1529050009667401
119	8	-0.15888960461267487
119	9	-0.1843720267253275
119	10	-0.19650207944573594
119	11	-0.1965295741164681
119	12	-0.22429343963315063
119	13	-0.18814662573718266
119	14	-0.22270758743010077
119	15	-0.2307916269518202
119	16	-0.22359584649045416
119	17	-0.24370753100931353
119	18	-0.24119226493072432
119	19	-0.2636051293073365
119	20	-0.27302115342821764
119	21	-0.25275547510935326
119	22	-0.259879977242889
119	23	-0.29185490439142825
119	24	-0.27134804093866904
119	25	-0.23402350279322803
119	26	-0.3143304779324154
119	27	-0.3224842175297269
119	28	-0.3123880916714725
119	29	-0.3417268619949496
119	30	-0.3777283237274105
119	31	-0.4475456268359963
119	32	-0.4693793430529425
119	33	-0.3896116257110478
119	34	-0.409939712085972
119	35	-0.4174075467948697
119	36	-0.4292443175476919
119	37	-0.4332508101215282
119	38	-0.45769736492142954
119	39	-0.4408644518964948
119	40	-0.44535301477105094
119	41	-0.45030754490522185
119	42	-0.4846246652180497
119	43	-0.5015341420828889
119	44	-0.491346001516348
119	45	-0.5529528084882855
119	46	-0.5114748341284638
119	47	-0.5010432424124431
119	48	-0.5084075640972188
119	49	-0.5266419171601484
119	50	-0.4915141736600006
120	1	-0.14409853619418556
120	2	-0.16515709432805892
120	3	-0.15357332951798242
120	4	-0.16077805610510154
120	5	-0.1586899070694372
120	6	-0.1828751009916587
120	7	-0.19741381833298277
120	8	-0.2035580882371093
120	9	-0.19397978491397083
120	10	-0.2117954436652525
120	11	-0.19748518804388482
120	12	-0.20622915855581425
120	13	-0.21005136650079237
120	14	-0.2238413424456951
120	15	-0.22143607473320515
120	16	-0.19921134063547186
120	17	-0.2320770184737384
120	18	-0.25207790270955854
120	19	-0.24806827049211932
120	20	-0.25141013709868787
120	21	-0.2560290625717201
120	22	-0.2607632359206207
120	23	-0.27992024171849295
120	24	-0.27109620484098684
120	25	-0.27341328810622656
120	26	-0.2046935426869041
120	27	-0.24546684086246984
120	28	-0.28259754456704655
120	29	-0.28020130425930745
120	30	-0.3378743063527878
120	31	-0.3510919881061554
120	32	-0.3717468201848675
120	33	-0.31035532955762335
120	34	-0.311069547041825
120	35	-0.32189555843084605
120	36	-0.30127620956150025
120	37	-0.30920475331208763
120	38	-0.3068907376286347
120	39	-0.2865946957656007
120	40	-0.30895988762620874
120	41	-0.3147159722246329
120	42	-0.3010258910407515
120	43	-0.30889477783873076
120	44	-0.3145305956968443
120	45	-0.3460094251355059
120	46	-0.31948081727742306
120	47	-0.2975127545367897
120	48	-0.33700821778376877
120	49	-0.30921189904761803
120	50	-0.28708410105881654
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14935.0
3	592.0
4	649.0
5	733.0
6	1343.0
7	2373.0
8	2909.0
9	3530.0
10	4738.0
11	7182.0
12	7904.0
13	10372.0
14	13655.0
15	16576.0
16	17549.0
17	19186.0
18	22833.0
19	27598.0
20	32602.0
21	30081.0
22	39233.0
23	49857.0
24	58903.0
25	66334.0
26	87298.0
27	114216.0
28	146818.0
29	160410.0
30	163848.0
31	198669.0
32	242601.0
33	311679.0
34	428026.0
35	630695.0
36	1013494.0
37	1858558.0
38	3629110.0
39	1.9004667E7
40	596526.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.799118988967958	28.24160886199355	25.183298764706265	26.775973384332225
2	24.434878724341267	29.211325609339983	25.38709965367523	20.96669601264352
3	23.310339455292077	26.897602765271316	26.41823227141216	23.373825508024453
4	25.233762107551676	25.949110901257868	25.127764101195794	23.689362889994666
5	25.619037192662674	27.422293268009618	25.977633947159422	20.981035592168283
6	25.61865057994822	26.43592000380739	23.65813514725148	24.28729426899291
7	24.097896011892164	26.7913060421412	26.252503505544855	22.858294440421787
8	24.161160773905287	26.43860611313025	25.99899677260521	23.401236340359254
9	23.76226320827107	25.75238094319767	26.42459013243277	24.060765716098494
10	23.495832157012593	27.611557736094717	25.043527024084966	23.849083082807724
11	24.4580619473287	26.455359859099104	25.39328256403047	23.69329562954172
12	23.790116095711173	26.184620701734353	25.92211550256313	24.103147699991343
13	23.998415883863625	26.57829364864132	25.861195267280856	23.562095200214202
14	24.00146193246934	26.76078887187418	25.820850183363152	23.416899012293328
15	23.281019173241724	26.362396370418885	26.617817817183536	23.738766639155855
16	23.67115244627764	26.277184717746042	26.425024042400302	23.62663879357601
17	23.55700091245099	26.200226377106738	26.00058295479841	24.24218975564386
18	23.275614921944925	26.77388009117207	25.961858827790724	23.98864615909228
19	24.344931976347635	26.231544965366755	25.906515406111147	23.517007652174467
20	23.629355388562804	26.341829172712284	26.058740517206736	23.97007492151818
21	23.63076438199753	26.757619476248628	25.98076566650878	23.630850475245058
22	23.971202272973343	26.32482437655364	26.04109873785843	23.662874612614583
23	23.898045923008134	26.20204116569027	26.408844539355403	23.49106837194619
24	23.530083097902647	26.56010071245813	25.982590280149914	23.92722590948931
25	23.77197527313251	26.533793788538862	25.932219787810684	23.76201115051794
26	23.86063662510611	26.081710535632762	26.60569012170604	23.451962717555087
27	24.2740744215128	26.074387488293745	26.051107563103574	23.600430527089877
28	23.733939344639094	26.26099119863791	25.939159906989246	24.065909549733746
29	23.96656278900031	26.085566615910295	26.019058229399416	23.928812365689982
30	24.075694633286467	26.24263447228148	25.990408110917606	23.691262783514443
31	23.83291716510138	26.52352614713741	26.008296042926315	23.635260644834897
32	24.131928426698458	26.089065239787917	25.95405038536437	23.824955948149253
33	23.84784350893313	26.46998979552829	25.938361960153998	23.743804735384582
34	23.897698037780124	26.016694785661414	26.30279251288413	23.782814663674333
35	23.630231555844027	26.181079867476125	26.14453172328633	24.04415685339352
36	24.09838130306001	26.082122731998425	26.088594089491195	23.73090187545037
37	24.141655339172	26.127757470162283	25.836376203723177	23.894210986942536
38	23.645575913928543	26.859957609846717	25.976603823310917	23.51786265291382
39	23.9317765026391	26.451898786361983	25.875179554235377	23.741145156763544
40	24.076046581307168	26.054532504553467	25.898164122427016	23.97125679171235
41	23.70718487899422	26.75074687718853	25.863442105213124	23.678626138604123
42	24.39692160097057	25.957702960181084	25.85748480996008	23.787890628888267
43	23.894157712956854	26.032760314274185	25.938878962007983	24.134203010760984
44	24.113529065888716	26.37804346228945	25.919462875962523	23.588964595859306
45	24.063943971358285	26.203727433624785	25.900189820361735	23.8321387746552
46	23.737293034143455	26.159279365572864	26.38173146135227	23.72169613893141
47	23.9641981575229	26.108000808126953	26.25647657062111	23.671324463729036
48	23.645508613932055	25.99184582644436	25.772407883922284	24.5902376757013
49	23.781843415245707	26.545239753168126	25.697010959335792	23.975905872250376
50	24.40135047247935	26.140509213967423	25.844799695761328	23.6133406177919
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6360.0
1	4619.5
2	2879.0
3	2684.0
4	2489.0
5	2399.0
6	2309.0
7	2456.0
8	2603.0
9	3114.0
10	3625.0
11	5541.5
12	7458.0
13	10360.0
14	13262.0
15	20230.0
16	27198.0
17	40929.5
18	54661.0
19	74740.5
20	94820.0
21	127021.0
22	159222.0
23	213458.5
24	267695.0
25	313776.0
26	359857.0
27	422291.5
28	484726.0
29	545123.5
30	605521.0
31	689109.5
32	772698.0
33	866852.5
34	961007.0
35	1063564.5
36	1166122.0
37	1268286.5
38	1370451.0
39	1472109.5
40	1573768.0
41	1646775.0
42	1719782.0
43	1768489.0
44	1817196.0
45	1881998.5
46	1946801.0
47	1989825.0
48	2032849.0
49	2042870.5
50	2052892.0
51	2024389.5
52	1995887.0
53	1941765.5
54	1887644.0
55	1811002.0
56	1734360.0
57	1672838.5
58	1611317.0
59	1475615.5
60	1339914.0
61	1150932.5
62	961951.0
63	820940.5
64	679930.0
65	568630.5
66	457331.0
67	387770.5
68	318210.0
69	262549.0
70	206888.0
71	164768.5
72	122649.0
73	101719.0
74	80789.0
75	65066.0
76	49343.0
77	41042.5
78	32742.0
79	27455.0
80	22168.0
81	17448.5
82	12729.0
83	10404.5
84	8080.0
85	5969.0
86	3858.0
87	2852.0
88	1846.0
89	1567.0
90	1288.0
91	1025.0
92	762.0
93	460.0
94	158.0
95	124.5
96	91.0
97	61.5
98	32.0
99	48.0
100	64.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5177131346820036
2	0.002748096461078517
3	0.0014050417996491664
4	0.0
5	0.06370555944046552
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.410610930770629E-5
14	1.721864950550449E-5
15	0.0
16	0.0
17	3.443729901100898E-5
18	1.0331189703302695E-5
19	0.0
20	9.986816713192604E-5
21	0.0
22	6.198713821981617E-5
23	2.445048229781638E-4
24	0.0012569614139018278
25	0.014170948543030198
26	0.008326938900861973
27	0.0013361672016271487
28	0.005647717037805474
29	0.009800855298533157
30	0.005737254015234097
31	0.00532745015700309
32	0.005086389063926027
33	0.00685990996299299
34	0.00946681349812637
35	0.008981247582071143
36	0.009101778128609675
37	0.011953186486721218
38	0.012772794203183231
39	0.01096139227520416
40	0.01693970738351532
41	0.011667356904929845
42	0.01590658841318505
43	0.01295531188794158
44	0.018716672012483383
45	0.013737038575491484
46	0.023634318311255467
47	0.011157684879566912
48	0.015706852078921196
49	0.030883369753072862
50	0.029071967825093782
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.9038282E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.955685099369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.80828242015026	40.42582619544096
2	11.652223607608857	12.107985209287184
3	4.014627278458896	6.257481320128415
4	1.902369473257991	3.9535563718097864
5	1.11704198380575	2.9018340776693
6	0.7335156568503605	2.2866185089664484
7	0.48520421377007417	1.7646382137667425
8	0.3392484692479969	1.4100709310953519
9	0.2818122510674075	1.3177573716242286
>10	1.495667006583649	13.770568014905372
>50	0.0852999577805858	3.0961196656062935
>100	0.0805480349758108	8.33786311839502
>500	0.0037369122354875793	1.2262703873236138
>1k	3.630642302687993E-4	0.2467895803360743
>5k	2.6519989603246863E-5	0.09114571871632024
>10k+	3.3149986982463996E-5	0.8054753149287571
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	115855	0.39897332769204463	Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	61087	0.21036712846855057	Illumina Paired End PCR Primer 2 (100% over 31bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.100675239671548E-4	6.198713821981617E-5	0.0	6.887459802201797E-6	3.4437299011008987E-6
2	2.2039871367045751E-4	1.1019935683522876E-4	0.0	1.0331189703302695E-5	1.0331189703302695E-5
3	2.272861734726593E-4	1.4808038574733864E-4	0.0	1.3774919604403595E-5	1.721864950550449E-5
4	2.9271704159357636E-4	1.997363342638521E-4	0.0	1.721864950550449E-5	2.754983920880719E-5
5	7.886141473521058E-4	2.513922827803656E-4	0.0	1.721864950550449E-5	4.132475881321078E-5
6	8.058327968576102E-4	2.961607714946773E-4	0.0	1.721864950550449E-5	5.854340831871528E-5
7	8.299389061653165E-4	3.1682315090128267E-4	0.0	1.721864950550449E-5	6.887459802201796E-5
8	8.574887453741238E-4	3.4437299011008985E-4	0.0	1.721864950550449E-5	7.576205782421976E-5
9	0.001019344050725866	3.753665592199979E-4	0.0	1.721864950550449E-5	8.953697742862335E-5
10	0.0018837202559021916	4.0636012832990603E-4	0.0	1.721864950550449E-5	1.0331189703302695E-4
11	0.0021419999984847587	4.476848871431168E-4	0.0	1.721864950550449E-5	1.1708681663743055E-4
12	0.0024312733101772344	4.649035366486213E-4	0.0	2.4106109307706288E-5	1.3086173624183415E-4
13	0.002675778133155398	4.924533758574285E-4	0.0	3.0993569109908084E-5	1.5496784554954043E-4
14	0.002816971059100535	5.09672025362933E-4	0.0	3.0993569109908084E-5	1.8251768475834761E-4
15	0.012989749186952589	5.578842439783456E-4	0.0	3.443729901100898E-5	1.8940514456054942E-4
16	0.013351340826568183	5.854340831871527E-4	0.0	3.443729901100898E-5	2.100675239671548E-4
17	0.014201942112140105	6.336463018025653E-4	0.0	3.788102891210988E-5	2.410610930770629E-4
18	0.014928569121272394	6.887459802201797E-4	0.0	4.8212218615412575E-5	2.445048229781638E-4
19	0.01632672346111936	7.231832792311887E-4	0.0	5.1655948516513474E-5	2.513922827803656E-4
20	0.23700093552366494	7.713954978466012E-4	0.0	5.509967841761438E-5	2.6516720238476917E-4
21	0.24231805449096472	8.127202566598121E-4	0.0	5.854340831871528E-5	2.7549839208807186E-4
22	0.2429826943618772	8.471575556708211E-4	0.0	5.854340831871528E-5	2.9271704159357636E-4
23	0.24364733423278967	8.678199350774264E-4	0.0	5.854340831871528E-5	3.1337942100018173E-4
24	0.2448664146177794	8.988135041873345E-4	0.0	6.887459802201796E-5	3.237106107034845E-4
25	0.24538641783284562	9.332508031983435E-4	0.0	6.887459802201796E-5	3.3059807050568623E-4
26	0.24597529564593387	9.84906751714857E-4	0.0	6.887459802201796E-5	3.3404180040678716E-4
27	0.24677079725308818	0.0010400064301324713	0.0	6.887459802201796E-5	3.40929260208989E-4
28	0.3295098518569384	0.001088218648747884	0.0	6.887459802201796E-5	3.4781672001119073E-4
29	0.3318963566784013	0.0011226559477588928	0.0	7.576205782421976E-5	3.6159163961559435E-4
30	0.34314357853539684	0.0011605369766710027	0.0	8.264951762642156E-5	3.7192282931889704E-4
31	0.7566115653811751	0.0011915305457809108	0.0	8.609324752752246E-5	3.822540190221997E-4
32	0.7597832406200891	0.0012397427643963234	0.0	8.609324752752246E-5	3.994726685277042E-4
33	0.7708686071717328	0.0012913987129128369	0.0	8.609324752752246E-5	4.098038582310069E-4
34	0.7714402663353156	0.0013361672016271487	0.0	9.298070732972426E-5	4.166913180332087E-4
35	0.7721152373959314	0.0013464983913304513	0.0	9.642443723082515E-5	4.201350479343096E-4
36	0.7727764335369427	0.0013981543398469648	0.0	9.642443723082515E-5	4.4079742734091503E-4
37	0.7735133917357783	0.0014463665584623774	0.0	9.986816713192606E-5	4.4079742734091503E-4
38	0.7742400187449106	0.0014704726677700836	0.0	1.0331189703302695E-4	4.511286170442177E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	3615	0.0	24.709236	43
ACCGATA	3195	0.0	23.13389	33
GACCGAT	3970	0.0	23.105497	32
GATATCG	3235	0.0	22.916595	36
CGATATC	3365	0.0	22.09652	35
TGCCGAG	29485	0.0	21.575094	26
GCCGAGA	29565	0.0	21.464819	27
AGATCGG	24670	0.0	21.381668	31
CGAGATC	24730	0.0	21.374487	29
GAGATCG	25020	0.0	21.152788	30
ATGCCGA	30215	0.0	21.083393	25
CCGATAT	3735	0.0	20.025335	34
CCGAGAT	27030	0.0	19.669617	28
AATGCCG	33060	0.0	19.182287	24
GAGACCG	5260	0.0	17.856709	30
TCGTATG	5200	0.0	17.768658	40
AGACCGA	5330	0.0	17.746096	31
CGAGACC	5560	0.0	16.933043	29
ATCGTAT	4440	0.0	16.895327	39
GATCGGA	38695	0.0	16.848152	1
>>END_MODULE