FastQCFastQC Report
Fri 27 May 2016
SRR522040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26123825
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1199540.4591747188629537Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG636600.24368560117057897Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369910.14159871305216598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33300.024.18171943
GATATCG28350.022.65901636
TGCCGAG288300.022.20908426
GACCGAT36750.022.02728532
CGAGATC247300.022.01476729
ATGCCGA292150.021.8646725
GCCGAGA294450.021.82751327
GAGATCG250900.021.69037430
AGATCGG251750.021.59063731
ACCGATA30050.021.44854433
CGATATC30900.020.93112835
CCGAGAT268750.020.37967728
AATGCCG330750.019.31946624
CCGATAT36150.018.13437534
AAGCAGT175050.017.805521
ATGCCGT45600.017.70770844
TCGTATG47000.017.17830340
GAGACCG49800.016.87337730
ATCGTAT39150.016.85753439
GATCGGA391750.016.8046591
Produced by FastQC (version 0.11.5)