##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522040_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26123825
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.002472493978196	40.0	39.0	40.0	38.0	40.0
2	38.977314233271734	40.0	39.0	40.0	38.0	40.0
3	38.975146097479985	40.0	39.0	40.0	38.0	40.0
4	38.95035057079122	40.0	39.0	40.0	38.0	40.0
5	38.924836734283744	40.0	39.0	40.0	38.0	40.0
6	39.0317281255712	40.0	39.0	40.0	38.0	40.0
7	39.01437052192778	40.0	39.0	40.0	38.0	40.0
8	39.020423540580296	40.0	39.0	40.0	38.0	40.0
9	39.015380787461254	40.0	39.0	40.0	38.0	40.0
10	38.9992748764777	40.0	39.0	40.0	38.0	40.0
11	39.09566397723151	40.0	39.0	40.0	38.0	40.0
12	39.06230194085284	40.0	39.0	40.0	38.0	40.0
13	39.045621956202815	40.0	39.0	40.0	38.0	40.0
14	39.02920697868708	40.0	39.0	40.0	38.0	40.0
15	39.00353749881574	40.0	39.0	40.0	38.0	40.0
16	39.009225142183425	40.0	39.0	40.0	38.0	40.0
17	38.9657711303762	40.0	39.0	40.0	38.0	40.0
18	38.93618618253644	40.0	39.0	40.0	37.0	40.0
19	38.913598640321624	40.0	39.0	40.0	37.0	40.0
20	38.874262363953214	40.0	39.0	40.0	37.0	40.0
21	38.82131816454903	40.0	39.0	40.0	37.0	40.0
22	38.776094542051176	40.0	39.0	40.0	36.0	40.0
23	38.72797670325843	40.0	39.0	40.0	36.0	40.0
24	38.68216890137643	40.0	39.0	40.0	36.0	40.0
25	38.58735571839116	40.0	39.0	40.0	36.0	40.0
26	38.45580614630514	40.0	39.0	40.0	35.0	40.0
27	38.40213004795431	40.0	38.0	40.0	35.0	40.0
28	38.35128071788875	40.0	38.0	40.0	35.0	40.0
29	38.28423781739466	40.0	38.0	40.0	35.0	40.0
30	38.25624310375682	40.0	38.0	40.0	35.0	40.0
31	38.15724270852373	40.0	38.0	40.0	35.0	40.0
32	38.08098492468082	40.0	38.0	40.0	35.0	40.0
33	38.19136114255856	40.0	38.0	40.0	35.0	40.0
34	38.151020265983256	40.0	38.0	40.0	35.0	40.0
35	38.09358667806112	40.0	38.0	40.0	35.0	40.0
36	38.125355073386075	40.0	38.0	40.0	35.0	40.0
37	38.03942986909459	40.0	38.0	40.0	35.0	40.0
38	38.24261562003267	40.0	39.0	40.0	35.0	40.0
39	38.20977946376536	40.0	39.0	40.0	35.0	40.0
40	38.17221463549078	40.0	39.0	40.0	35.0	40.0
41	38.24644434725772	40.0	39.0	40.0	35.0	40.0
42	38.19755009076963	40.0	39.0	40.0	35.0	40.0
43	38.14301217375327	40.0	39.0	40.0	35.0	40.0
44	38.09599807838247	40.0	39.0	40.0	35.0	40.0
45	38.04013236958983	40.0	39.0	40.0	35.0	40.0
46	37.93665850999997	40.0	38.0	40.0	35.0	40.0
47	37.880678499415765	40.0	38.0	40.0	35.0	40.0
48	37.79749263363998	40.0	38.0	40.0	35.0	40.0
49	37.722564708651966	40.0	38.0	40.0	34.0	40.0
50	37.620116770802134	40.0	38.0	40.0	34.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1754496424154297
1	2	-0.19751468869164057
1	3	-0.18686546034707163
1	4	-0.18572854899161229
1	5	-0.195843950084722
1	6	-0.18332410331993287
1	7	-0.1922064908279424
1	8	-0.18911713174058775
1	9	-0.19218000309951577
1	10	-0.19255498238981517
1	11	-0.2088335088194313
1	12	-0.22223250844048437
1	13	-0.2100095151691974
1	14	-0.21334660972447494
1	15	-0.2175991562060915
1	16	-0.24520667065510082
1	17	-0.2026029424826703
1	18	-0.2037806860799023
1	19	-0.2195638722977904
1	20	-0.2366445709449394
1	21	-0.1930645535258364
1	22	-0.20681765883324488
1	23	-0.20495055358626502
1	24	-0.24181728664193258
1	25	-0.2819789050554533
1	26	-0.25794247683226956
1	27	-0.2262950992419448
1	28	-0.2561179323724403
1	29	-0.297368998041172
1	30	-0.32085177172395163
1	31	-0.24879584861459136
1	32	-0.23222603838129174
1	33	-0.18614709538650942
1	34	-0.1531467423116979
1	35	-0.1461698188647702
1	36	-0.14621923558555494
1	37	-0.1597353566692803
1	38	-0.14780504573322872
1	39	-0.10981440992814129
1	40	-0.12221314261018534
1	41	-0.09600547055956099
1	42	-0.0989944842813486
1	43	-0.11319495624047704
1	44	-0.10616632022640715
1	45	-0.15463009404400196
1	46	-0.12536360586206285
1	47	-0.11720057242850856
1	48	-0.14291494940294314
1	49	-0.09203259166834954
1	50	-0.08326098124980064
2	1	-0.20146055467635193
2	2	-0.2476395566775338
2	3	-0.31286489658700134
2	4	-0.323995562946898
2	5	-0.3213576054507996
2	6	-0.25282183498504907
2	7	-0.21550710134105344
2	8	-0.20347127244022545
2	9	-0.22012122260106537
2	10	-0.20499414450324593
2	11	-0.2191917102119234
2	12	-0.2078162300214359
2	13	-0.2054135260536185
2	14	-0.22535301854136236
2	15	-0.19786826480454778
2	16	-0.195927222993447
2	17	-0.22124870994900903
2	18	-0.19416503883770986
2	19	-0.21456355059859078
2	20	-0.21694715091219052
2	21	-0.2333199270983357
2	22	-0.23709639255107362
2	23	-0.23963089942863292
2	24	-0.23782921636006904
2	25	-0.3488124459095516
2	26	-0.23933345590406674
2	27	-0.19358771972088107
2	28	-0.22745795541153768
2	29	-0.2612906418555596
2	30	-0.2722586961300735
2	31	-0.27742278515004415
2	32	-0.26599966221007776
2	33	-0.1997414786779359
2	34	-0.1859152080504316
2	35	-0.19617014853642445
2	36	-0.1803894824217096
2	37	-0.20069773156696868
2	38	-0.19620285228337053
2	39	-0.21429176465315436
2	40	-0.14780472976887893
2	41	-0.15925545902105398
2	42	-0.1703543436421313
2	43	-0.20674321739058854
2	44	-0.17789617891534704
2	45	-0.20649473629402593
2	46	-0.2001158554616893
2	47	-0.17163972187219656
2	48	-0.19938202337139188
2	49	-0.28284018680329126
2	50	-0.23391681882760196
3	1	-0.16705329133456814
3	2	-0.2063081996184053
3	3	-0.2516905870298274
3	4	-0.2636290863529567
3	5	-0.2021147557347618
3	6	-0.279905202630772
3	7	-0.2075435242692265
3	8	-0.2353051126421235
3	9	-0.21002186645661425
3	10	-0.18482273890790424
3	11	-0.16293869519729753
3	12	-0.17719990971296795
3	13	-0.1413752718583723
3	14	-0.1666506659169542
3	15	-0.15430134824821806
3	16	-0.1586718912063816
3	17	-0.1693002377274766
3	18	-0.17608228201216747
3	19	-0.18463448057053
3	20	-0.17987924830733704
3	21	-0.16585394887542293
3	22	-0.19616676493178176
3	23	-0.17382577505547658
3	24	-0.22707688651908597
3	25	-0.23235234877028432
3	26	-0.33929347152903233
3	27	-0.20847827275064645
3	28	-0.2729186204059033
3	29	-0.2094482307174843
3	30	-0.17695297394560328
3	31	-0.17414518527951373
3	32	-0.16327826913428112
3	33	-0.09787086574053205
3	34	-0.10588212374745609
3	35	-0.09851747989193171
3	36	-0.0869786335248719
3	37	-0.04339582265245667
3	38	-0.05733943611575398
3	39	-0.05655819992901456
3	40	-0.03634044771174416
3	41	-0.08149966106289952
3	42	-0.0729503980397368
3	43	-0.03686408471705249
3	44	-0.07635103240562557
3	45	-0.11592810688762967
3	46	-0.07178586803374287
3	47	-0.07908844305247698
3	48	-0.07260973780353197
3	49	-0.20615556491149079
3	50	-0.1439530282432031
4	1	-0.15332953353901502
4	2	-0.27810990695967064
4	3	-0.14736481328202444
4	4	-0.1290446534365728
4	5	-0.13129064608563112
4	6	-0.17102044626059865
4	7	-0.14778263489331067
4	8	-0.136070944074973
4	9	-0.1288991890052955
4	10	-0.13518015154643592
4	11	-0.11901202245778819
4	12	-0.12226494511859443
4	13	-0.1159379576932551
4	14	-0.12656573750175681
4	15	-0.12787565739851203
4	16	-0.14391767421248147
4	17	-0.12489847503558593
4	18	-0.1227469339011833
4	19	-0.1496515558217908
4	20	-0.14596394912475574
4	21	-0.1624419091919762
4	22	-0.14019353770862608
4	23	-0.17761920568908351
4	24	-0.15493579768413923
4	25	-0.21236297817796412
4	26	-0.14060819537581182
4	27	-0.20018608456562248
4	28	-0.17732525364383633
4	29	-0.26129670190019993
4	30	-0.1623932902168903
4	31	-0.24552710246124576
4	32	-0.14112882619967593
4	33	-0.08419571386480129
4	34	-0.15444002971256054
4	35	-0.08760238477050564
4	36	-0.07910463737143658
4	37	-0.14037315755755486
4	38	-0.10065034711540477
4	39	-0.07528921827854163
4	40	-0.10110189768050049
4	41	-0.07114120455763384
4	42	-0.028723554256096406
4	43	-0.0757554185556657
4	44	-0.06483636990747499
4	45	-0.101133743397817
4	46	-0.03519793273930816
4	47	-0.04164364026073741
4	48	-0.08004924254240109
4	49	-0.06577128673772137
4	50	-0.038755789240880745
5	1	-0.1412534412312496
5	2	-0.18297115283856158
5	3	-0.15165058347395188
5	4	-0.12454294781835529
5	5	-0.14332018025623228
5	6	-0.17321491487529528
5	7	-0.1454687370198755
5	8	-0.14204692738415758
5	9	-0.13556873520222013
5	10	-0.14289848437625352
5	11	-0.1628725567216378
5	12	-0.17468811492605596
5	13	-0.1759354709478913
5	14	-0.16520840757501531
5	15	-0.1599135475400857
5	16	-0.14398275861804422
5	17	-0.14544342098895413
5	18	-0.13199590158989594
5	19	-0.15398942998530885
5	20	-0.14589385169258406
5	21	-0.1432770003535424
5	22	-0.14276309221735062
5	23	-0.13620217904767884
5	24	-0.14050316897807846
5	25	-0.15140511048906546
5	26	-0.06830673802442533
5	27	-0.11737285711885903
5	28	-0.09990968136700218
5	29	-0.15484339537054836
5	30	-0.1357194806307902
5	31	-0.11249289721854439
5	32	-0.11320373212203094
5	33	-0.056113704023516675
5	34	-0.006585181944402052
5	35	-0.026978511910002112
5	36	-0.016029337356620488
5	37	-0.0019650680955081157
5	38	-0.010226764590377968
5	39	-0.025684184853886904
5	40	-0.008446832613238087
5	41	-0.02879864749213823
5	42	-0.04042896212573055
5	43	-0.004493748790324048
5	44	-0.06421343417098768
5	45	-0.050102574943473144
5	46	-0.013036551335737556
5	47	-0.004736941457984756
5	48	-0.02777318020160635
5	49	0.042913468358015905
5	50	-0.005197815379595738
6	1	-0.08443932782822827
6	2	-0.09138174376813879
6	3	-0.11000028431749342
6	4	-0.07460979362283382
6	5	-0.12635601097483118
6	6	-0.08864651377839294
6	7	-0.09090911551126624
6	8	-0.1041458298195792
6	9	-0.08096944967517317
6	10	-0.0952101436406636
6	11	-0.0899738198043778
6	12	-0.09988229988677233
6	13	-0.0897213157325254
6	14	-0.09687862247829315
6	15	-0.1082115104711221
6	16	-0.09962124017391716
6	17	-0.0990400633562416
6	18	-0.10787418502074786
6	19	-0.10987113630773138
6	20	-0.1193865421537339
6	21	-0.12157948699613996
6	22	-0.12649604620790456
6	23	-0.12153892427483726
6	24	-0.1428312464552306
6	25	-0.2589579619233433
6	26	-0.10907428931338359
6	27	-0.03655941261295226
6	28	-0.04626217860695192
6	29	-0.13029223041759508
6	30	-0.07955071746133058
6	31	-0.0455170329007899
6	32	-0.07563065068976016
6	33	0.011007158045870824
6	34	0.011027909747127751
6	35	0.006056258759713273
6	36	0.07370399511529513
6	37	0.04647891500115975
6	38	0.03746973663987063
6	39	0.04731634811862051
6	40	0.05362476572282304
6	41	0.03360211170357985
6	42	0.033706227727456906
6	43	0.017459114034771517
6	44	0.01115218703866816
6	45	0.023145686435228185
6	46	0.0049705335542924445
6	47	0.002925116965009522
6	48	-0.02174784039164024
6	49	0.02893969070571245
6	50	-0.04177779722829911
7	1	-0.07430559468252085
7	2	-0.06968216630419732
7	3	-0.03966829864999255
7	4	-0.07005766190123097
7	5	-0.03676624557249397
7	6	-0.03363517764843493
7	7	-0.10879360329382592
7	8	-0.08035696831619532
7	9	-0.08844461819965233
7	10	-0.08886145398769685
7	11	-0.08195381309081284
7	12	-0.09473690714666816
7	13	-0.08084886846914685
7	14	-0.08885515506354125
7	15	-0.08871924441913137
7	16	-0.08985511604242902
7	17	-0.0875014411039956
7	18	-0.07624582253244228
7	19	-0.08920222074197426
7	20	-0.07991735631165398
7	21	-0.07510561495509904
7	22	-0.08815923036674178
7	23	-0.09682232498384025
7	24	-0.06649742229181044
7	25	-0.12818117099960347
7	26	-0.11294689330843255
7	27	-0.11349258735896228
7	28	-0.13702050214970285
7	29	-0.11651642042485122
7	30	-0.05845395286863919
7	31	-0.06702444778802885
7	32	-0.05830690127199745
7	33	-0.018324113234484685
7	34	-0.029092541957687956
7	35	0.0025024234428698833
7	36	0.041576191534993256
7	37	0.06973330295674174
7	38	0.03413409377003518
7	39	0.05732635484721271
7	40	0.06349063107228403
7	41	0.0509485922131816
7	42	0.07615315008827395
7	43	0.06659601150911243
7	44	0.08400420768160899
7	45	0.08141825083477272
7	46	0.13115301823887648
7	47	0.09223853744068577
7	48	0.09499821862296898
7	49	0.1122988779639158
7	50	0.10421761445977751
8	1	-0.10992964535441274
8	2	-0.1669937312222558
8	3	-0.2105948278920664
8	4	-0.13029097323934735
8	5	-0.11666654917830499
8	6	-0.13462584904680597
8	7	-0.11218644424255331
8	8	-0.1224891241530841
8	9	-0.13148505432344848
8	10	-0.12385030712054856
8	11	-0.12023998828590976
8	12	-0.13479727818399567
8	13	-0.13150965003405446
8	14	-0.12646381141590268
8	15	-0.1138400488852227
8	16	-0.11806266062932735
8	17	-0.12306507068386452
8	18	-0.11057996451814489
8	19	-0.11829721308593832
8	20	-0.09973366660560856
8	21	-0.10456872479262813
8	22	-0.07297778158916657
8	23	-0.11284721456846114
8	24	-0.12994402750931755
8	25	-0.11915263854346847
8	26	-0.06729235106369202
8	27	-0.05543469911783916
8	28	-0.05577987809499518
8	29	-0.07654313200013974
8	30	-0.10640801748774464
8	31	-0.07212873285573096
8	32	-0.09137418192483437
8	33	-0.025768530598860195
8	34	-0.005065903714452702
8	35	-0.025503384020503006
8	36	1.2985248876873356E-4
8	37	0.0057490392887658
8	38	-0.05160530320330281
8	39	-0.024452764047374842
8	40	-0.01589761724937233
8	41	0.0018689308226029766
8	42	-0.0041588822845994855
8	43	-0.014897763490665739
8	44	0.0024708441196636954
8	45	0.010018412369227292
8	46	0.04280425843756319
8	47	0.03890200624753959
8	48	0.06621288819564342
8	49	0.08827467972590597
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114	11	-0.031028069192664987
114	12	-0.007053695015727612
114	13	-0.028999121917401283
114	14	-0.04872704617449841
114	15	-0.03994923110898441
114	16	-0.009532853840177324
114	17	-0.021029838845940674
114	18	-0.006472017183000389
114	19	-0.03004792365347697
114	20	-0.039943073828268894
114	21	-0.006997186820228762
114	22	-0.019623965085138195
114	23	-0.01693253772637604
114	24	0.0068172183453540924
114	25	-0.011657192724747745
114	26	-0.01577808678419501
114	27	0.023877711662862566
114	28	-0.025168398955557336
114	29	0.0153665124165272
114	30	0.0012132734909897636
114	31	-0.006867691572068679
114	32	-0.02673583666124557
114	33	0.013499445012392641
114	34	0.017101374815062798
114	35	0.006248648835779136
114	36	0.0032246461979141827
114	37	0.00193043589975872
114	38	0.0014603265896226958
114	39	-0.032840975196513966
114	40	-0.001139308607264411
114	41	-0.026816150350363444
114	42	-0.02519636944945347
114	43	-0.014206035048140109
114	44	-0.03955122803720457
114	45	-0.029950085475697108
114	46	0.010835790097814879
114	47	-0.004692746988261831
114	48	0.004620969130016306
114	49	0.014906682544811645
114	50	5.024317085542407E-4
115	1	-0.030362304651291083
115	2	-0.027492839050189843
115	3	-2.7089154498582957E-4
115	4	-0.029040163547826126
115	5	-0.011739428694937715
115	6	-0.027266849695088524
115	7	-0.05120051435395112
115	8	-0.037000043690106565
115	9	-0.03778909626370108
115	10	-0.03480517653508741
115	11	-0.032549610568040066
115	12	-0.0452835662679405
115	13	-0.04487264637611332
115	14	-0.05893447699347831
115	15	-0.06233023446886676
115	16	-0.06025460266816651
115	17	-0.059125231224641084
115	18	-0.06969822803319659
115	19	-0.06764614757190657
115	20	-0.06219414606614038
115	21	-0.09061643249201268
115	22	-0.07613480177413834
115	23	-0.088907109405028
115	24	-0.09330285800577798
115	25	-0.09254959258937845
115	26	-0.07983205330334187
115	27	-0.059226292377715595
115	28	-0.09345893670055005
115	29	-0.08159135502758375
115	30	-0.13810794609416632
115	31	-0.11594459952511471
115	32	-0.12971367190065308
115	33	-0.05596844292314529
115	34	-0.05965295200759613
115	35	-0.06806536101771599
115	36	-0.10511452868210114
115	37	-0.06355285834921176
115	38	-0.10186348273081336
115	39	-0.0886765177500024
115	40	-0.08195331568737174
115	41	-0.09008641957124297
115	42	-0.07204405220082322
115	43	-0.10338821571244239
115	44	-0.06388447199347524
115	45	-0.037852101722265274
115	46	-0.06777473659677469
115	47	-0.13264896136654158
115	48	-0.10922239397125821
115	49	-0.10791741218930895
115	50	-0.13894262402109092
116	1	1.4710707473852835E-4
116	2	-0.009609455941550493
116	3	-0.0035339420277935574
116	4	-0.0014035920971053883
116	5	-0.0017082835572068689
116	6	0.002878019668948184
116	7	-0.021226781465962574
116	8	-0.019806878451021248
116	9	-0.02211533160847523
116	10	-0.034967649202691575
116	11	-0.054913409201205354
116	12	-0.04378130186368878
116	13	-0.04506157974458347
116	14	-0.04437533315014974
116	15	-0.051049173171776374
116	16	-0.072552458622404
116	17	-0.048775171873742806
116	18	-0.07941574974262267
116	19	-0.05035528154409974
116	20	-0.07941800903873286
116	21	-0.05795547219179298
116	22	-0.07364345707379982
116	23	-0.06493984380102091
116	24	-0.06371790378086217
116	25	-0.1208105240899755
116	26	-0.051507495159398786
116	27	-0.02084111961069368
116	28	-0.038342087954902127
116	29	-0.06286851088515277
116	30	-0.06475399570897622
116	31	-0.061022496726501174
116	32	-0.09220923438825679
116	33	-0.006730994529661416
116	34	-0.026702076009904374
116	35	-0.031186352554648522
116	36	-0.025666810521464356
116	37	-0.054417475447138486
116	38	-0.07385754402047695
116	39	-0.05262480642852552
116	40	-0.05892958863152842
116	41	-0.05521563752135705
116	42	-0.017384745380141453
116	43	-0.06326433992185088
116	44	-0.022093553412737776
116	45	-0.026232374287339155
116	46	-0.042465609022649176
116	47	-0.05689004685696375
116	48	-0.042706316289994106
116	49	-0.00779041996887031
116	50	-0.019762800576053507
117	1	-0.026886869519408663
117	2	-0.009795669708218213
117	3	-0.0326846258295177
117	4	-0.003225004487447336
117	5	-0.0074696653345895925
117	6	-0.013751350174473487
117	7	-0.0324303824393084
117	8	-0.028908894616790803
117	9	-0.028650091436240643
117	10	-0.03665013295685782
117	11	-0.06159978193340265
117	12	-0.047679091737641954
117	13	-0.05815882629309499
117	14	-0.06074187671205067
117	15	-0.0487227899576439
117	16	-0.06100435977084828
117	17	-0.05628962522512637
117	18	-0.06700197118898643
117	19	-0.08296783986580891
117	20	-0.03943124894175298
117	21	-0.07708765657329764
117	22	-0.06349824626790479
117	23	-0.1013524423587171
117	24	-0.10509245943924839
117	25	-0.03496744684979802
117	26	-0.06617490520307001
117	27	-0.08211989814118681
117	28	-0.08460157768885779
117	29	-0.06640248563117268
117	30	-0.14271572314995495
117	31	-0.10973688098984269
117	32	-0.09258690382176127
117	33	-0.08875943598304303
117	34	-0.08487535111828493
117	35	-0.10849801955787797
117	36	-0.11318873675497088
117	37	-0.09892367422509807
117	38	-0.10816761043923862
117	39	-0.13632379692298002
117	40	-0.09242786354445798
117	41	-0.11801962451926329
117	42	-0.13541949657888352
117	43	-0.1375836032328408
117	44	-0.1016088294865014
117	45	-0.11824607047832103
117	46	-0.12901469077204553
117	47	-0.11080358337242302
117	48	-0.1210196831580177
117	49	-0.1021234957699022
117	50	-0.11085698205224759
118	1	-0.05666967960126357
118	2	-0.051131606384053896
118	3	-0.035432584452038896
118	4	-0.018891018577960494
118	5	-0.017671593190634383
118	6	-0.03977640577052455
118	7	-0.03681909879348666
118	8	-0.05850597581503081
118	9	-0.06646596932794324
118	10	-0.05942235302746468
118	11	-0.06921233682676586
118	12	-0.07398351548655313
118	13	-0.08508798740947299
118	14	-0.07142925740480166
118	15	-0.07211828832612355
118	16	-0.09252636463677533
118	17	-0.09277457282905743
118	18	-0.08340445955551701
118	19	-0.09121639381460511
118	20	-0.11283630276025747
118	21	-0.10334619889198393
118	22	-0.11778336738660045
118	23	-0.12902447695213226
118	24	-0.0957798717074283
118	25	-0.050144129113697034
118	26	-0.08317405921445697
118	27	-0.13613789305681934
118	28	-0.12134524000816072
118	29	-0.10194606158908215
118	30	-0.13618745107865493
118	31	-0.14427111077553434
118	32	-0.17568114385238687
118	33	-0.10343183338333972
118	34	-0.09846125606339484
118	35	-0.10107783538822446
118	36	-0.129820260226019
118	37	-0.0991728095500477
118	38	-0.12332359397301218
118	39	-0.14018504182419633
118	40	-0.1580984961887495
118	41	-0.1401718597129502
118	42	-0.17597998143877192
118	43	-0.17750336242825426
118	44	-0.17391488037947056
118	45	-0.14583729008606383
118	46	-0.13233752281590938
118	47	-0.14491414269856762
118	48	-0.13597798055488397
118	49	-0.16021315218677046
118	50	-0.13227392704857266
119	1	-0.04251870815087244
119	2	-0.040477029069045045
119	3	-0.08511629552228328
119	4	-0.009594917614876408
119	5	-0.03166707285363657
119	6	-0.03522350100139704
119	7	-0.037785384104253694
119	8	-0.05817079084423682
119	9	-0.060395044150716615
119	10	-0.07075898640380984
119	11	-0.07653782883298277
119	12	-0.09445351071173036
119	13	-0.07505361918280329
119	14	-0.10809166357841349
119	15	-0.11411356126107819
119	16	-0.1020886809560011
119	17	-0.12493724623421087
119	18	-0.1160649354776524
119	19	-0.1285076965174028
119	20	-0.13129799357751182
119	21	-0.14879098432965066
119	22	-0.1277677493192968
119	23	-0.1389256720410117
119	24	-0.1370446140950392
119	25	-0.1310401630460234
119	26	-0.1141705083671809
119	27	-0.1269004471257773
119	28	-0.13435882377419262
119	29	-0.15435079945186203
119	30	-0.20646778142209854
119	31	-0.1906756261999334
119	32	-0.21668459906612014
119	33	-0.1577884992439138
119	34	-0.15874546536320366
119	35	-0.19174745846550678
119	36	-0.17516213985656037
119	37	-0.2240562335700389
119	38	-0.22258016785956158
119	39	-0.20094190152037328
119	40	-0.2153944128671199
119	41	-0.203822612870745
119	42	-0.26133041883755226
119	43	-0.24964337866351372
119	44	-0.24089689771981426
119	45	-0.2326836848479914
119	46	-0.305411203598247
119	47	-0.2625152906003265
119	48	-0.2640986062476287
119	49	-0.25235903236666246
119	50	-0.28144999482295674
120	1	-0.06704157891321927
120	2	-0.04789037316525224
120	3	-0.05380778546251719
120	4	-0.05647667004537027
120	5	-0.015570196923626156
120	6	-0.04717434033612733
120	7	-0.06633047801987857
120	8	-0.06672809623347575
120	9	-0.06549976305932859
120	10	-0.08670559590112248
120	11	-0.09717891679220969
120	12	-0.11598502062346938
120	13	-0.125107455550733
120	14	-0.0998679287810802
120	15	-0.13619781082508808
120	16	-0.14131278178541606
120	17	-0.12231080700124153
120	18	-0.13312969554416298
120	19	-0.12187303194696142
120	20	-0.14246574755593855
120	21	-0.12114263299263683
120	22	-0.1433514420319426
120	23	-0.11330390070745722
120	24	-0.12422820313261695
120	25	-0.09131718894474261
120	26	-0.10867912080547626
120	27	-0.13850340592309607
120	28	-0.07637315388807053
120	29	-0.16331709676907025
120	30	-0.1640587273082943
120	31	-0.17005072096431206
120	32	-0.19406487902483605
120	33	-0.148380730504158
120	34	-0.19531938709620533
120	35	-0.139786569755465
120	36	-0.13217323882795995
120	37	-0.14778997446470754
120	38	-0.14703583470002002
120	39	-0.1492541923109556
120	40	-0.1545735730245852
120	41	-0.1402067957710571
120	42	-0.1612573864940714
120	43	-0.16062348267713134
120	44	-0.13773879359071373
120	45	-0.1779937682814534
120	46	-0.15018720313053535
120	47	-0.16215982891310432
120	48	-0.1379167181967773
120	49	-0.13492864238823898
120	50	-0.1506557064120031
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15221.0
3	915.0
4	606.0
5	655.0
6	1142.0
7	1854.0
8	2269.0
9	2804.0
10	3871.0
11	5654.0
12	6157.0
13	8001.0
14	10697.0
15	12841.0
16	13973.0
17	15446.0
18	18398.0
19	21691.0
20	27379.0
21	24598.0
22	32618.0
23	41882.0
24	49688.0
25	53718.0
26	71576.0
27	94939.0
28	122605.0
29	133977.0
30	133811.0
31	162297.0
32	199490.0
33	256410.0
34	350696.0
35	520730.0
36	839573.0
37	1545302.0
38	3090528.0
39	1.754131E7
40	688503.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.528749269760077	28.13199336279151	25.258340049886037	27.08091731756238
2	24.32766606153703	29.198075375388616	25.305749711522207	21.168508851552144
3	23.436462509850234	26.677265454543225	26.367003624519032	23.519268411087506
4	25.099742495053356	25.68555763582362	25.146366699752754	24.06833316937027
5	25.59367065878905	27.30908394242666	26.070349896463835	21.026895502320446
6	25.811327399414136	26.280167624763983	23.58039835284458	24.3281066229773
7	24.138253108034526	26.735855105444934	26.313627502863767	22.812264283656777
8	24.306574554070853	26.41716134601269	25.878526594019064	23.397737505897396
9	23.93565643622249	25.632911719474468	26.34243262615639	24.088999218146654
10	23.48600941860543	27.609176680673674	25.059974946241603	23.844838954479293
11	24.53582888416991	26.462549033305805	25.302512170403833	23.69910991212045
12	23.799773723785613	26.075941255767148	25.915007695657422	24.209277324789817
13	24.021864969394187	26.505581348131795	25.738832136034134	23.73372154643988
14	24.059394058871547	26.816976457314347	25.712272226597747	23.411357257216352
15	23.269387235598156	26.387399241879777	26.603167797977516	23.740045724544547
16	23.70301439394882	26.159090408850926	26.36023246978572	23.777662727414533
17	23.662247183037103	26.098616391472433	25.876898953907567	24.362237471582894
18	23.270719352927834	26.7517601270105	25.859811876706416	24.11770864335525
19	24.429198251021816	26.252564469406757	25.796318111915078	23.521919167656343
20	23.740209442229368	26.258349341425376	25.945534577765912	24.055906638579344
21	23.649618690984187	26.821378569179664	25.887786340629678	23.64121639920647
22	24.12898345819705	26.289950376696513	25.91973645503155	23.661329710074888
23	23.965515109600123	26.115919053442838	26.434294533474134	23.484271303482906
24	23.51317753551692	26.65489707380029	25.871776384881002	23.960149005801796
25	23.803222940644876	26.496687698694625	25.844506478552177	23.85558288210832
26	23.884305689357863	25.98625900184445	26.694841904179746	23.434593404617946
27	24.327469437110445	26.002243857956568	26.06563826175538	23.604648443177602
28	23.723755244185334	26.192456075013347	25.940238307663492	24.143550373137828
29	23.95584337362542	26.012848761518136	26.049135030512964	23.98217283434348
30	24.117147293976917	26.18730236190704	26.006862797715137	23.688687546400907
31	23.82543580012171	26.517200811525193	26.020752392216608	23.636610996136493
32	24.17714520576954	26.01827482892493	25.981495645697645	23.823084319607887
33	23.872967316360334	26.448332192447584	25.95284503933663	23.725855451855452
34	23.90434490500814	25.947325958316704	26.383100818372267	23.765228318302885
35	23.590080617464785	26.09561030797018	26.21897723705014	24.0953318375149
36	24.11920178973959	26.003954017085125	26.155720038929953	23.72112415424533
37	24.170920437763503	26.061381863330908	25.84403679839958	23.92366090050601
38	23.629226482372335	26.85987466432593	25.99916858375144	23.511730269550295
39	23.92033737278588	26.457148945151122	25.901696943706646	23.720816738356355
40	24.116927159074148	25.988191558749698	25.89698338854355	23.997897893632604
41	23.69144728326466	26.771946249007154	25.882283039359898	23.654323428368286
42	24.450900310820064	25.915889724974754	25.85935123073253	23.773858733472643
43	23.895632535638104	25.985235936777464	25.92144083818123	24.197690689403203
44	24.14858437392361	26.37208052832062	25.907362424034808	23.57197267372096
45	24.083365051203018	26.194086870287354	25.907362688039882	23.815185390469754
46	23.718555048922088	26.107560831302234	26.471041352934687	23.70284276684099
47	23.97230740618276	26.083048609430627	26.299116811318928	23.645527173067684
48	23.62055899916777	25.938437182405544	25.77142456358076	24.669579254845928
49	23.747438556661784	26.56614524779869	25.678013577789134	24.008402617750388
50	24.4590560478595	26.09642895034878	25.836219749962545	23.60829525182917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7922.0
1	5612.5
2	3303.0
3	3133.5
4	2964.0
5	2778.5
6	2593.0
7	2683.0
8	2773.0
9	3222.0
10	3671.0
11	5451.5
12	7232.0
13	9830.5
14	12429.0
15	18614.5
16	24800.0
17	36687.0
18	48574.0
19	66531.5
20	84489.0
21	114013.0
22	143537.0
23	202043.5
24	260550.0
25	291383.0
26	322216.0
27	378031.5
28	433847.0
29	484999.0
30	536151.0
31	611087.0
32	686023.0
33	771761.0
34	857499.0
35	948177.0
36	1038855.0
37	1131649.0
38	1224443.0
39	1313941.0
40	1403439.0
41	1470309.5
42	1537180.0
43	1577626.0
44	1618072.0
45	1676556.5
46	1735041.0
47	1774621.0
48	1814201.0
49	1825577.5
50	1836954.0
51	1817073.5
52	1797193.0
53	1747179.5
54	1697166.0
55	1630608.0
56	1564050.0
57	1518725.5
58	1473401.0
59	1350212.5
60	1227024.0
61	1052648.0
62	878272.0
63	750555.5
64	622839.0
65	520919.5
66	419000.0
67	352967.0
68	286934.0
69	238255.5
70	189577.0
71	153095.0
72	116613.0
73	96990.0
74	77367.0
75	61968.5
76	46570.0
77	39059.5
78	31549.0
79	26383.0
80	21217.0
81	16678.0
82	12139.0
83	9861.5
84	7584.0
85	5445.5
86	3307.0
87	2438.5
88	1570.0
89	1247.0
90	924.0
91	716.5
92	509.0
93	316.5
94	124.0
95	91.0
96	58.0
97	42.5
98	27.0
99	40.0
100	53.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.519797541133429
2	0.001902477910489754
3	3.177176389751501E-4
4	2.1053578486305124E-4
5	0.05769828882255948
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	3.8279233611463865E-6
13	1.110097774732452E-4
14	0.0
15	0.0
16	0.0
17	1.3014939427897715E-4
18	0.0
19	0.0
20	2.220195549464904E-4
21	0.0
22	3.8279233611463863E-5
23	0.0010258834607872315
24	3.8279233611463863E-5
25	0.010377500232067854
26	0.004298757934567392
27	2.602987885579543E-4
28	0.00140867579690187
29	0.003812611667701801
30	0.0036403551164502137
31	0.002557052805245786
32	0.001347429023123528
33	0.0032422510868909894
34	0.007081658218120814
35	0.009807139651257043
36	0.007793651963294043
37	0.010293285918122634
38	0.008161132605964096
39	0.008482678168300391
40	0.012854166646729566
41	0.012073270281055704
42	0.013658030552570307
43	0.010320081381650657
44	0.013298205756622546
45	0.011112461517407959
46	0.015813151404895722
47	0.011123945287491398
48	0.013945124804656286
49	0.027568704046976273
50	0.024414495197391653
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.6123825E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.12140279325328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83701069487776	40.56974186650488
2	11.644720894016462	12.138783762640891
3	3.9896702391539764	6.238416286415988
4	1.8939199890256226	3.948550664247933
5	1.1187769536160028	2.9156112117614263
6	0.7267725691127865	2.2728243488289035
7	0.49811227295241445	1.8173617290371
8	0.3653949401397719	1.5235917482913377
9	0.2727428034826896	1.2794163767354148
>10	1.4804051019155113	13.691816629719517
>50	0.08594663194202144	3.144305059637311
>100	0.08391202296353505	8.453994955025419
>500	0.0023263436228556852	0.7777097094431022
>1k	2.2981607971842317E-4	0.17231856708572557
>5k	2.2022662084295416E-5	0.08212116119199017
>10k+	3.670443680712915E-5	0.9734359234330889
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	119954	0.4591747188629537	Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	63660	0.24368560117057897	Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36991	0.14159871305216598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0240594553056455E-4	1.9139616805731932E-5	0.0	0.0	3.8279233611463865E-6
2	3.406851791420284E-4	5.7418850417195795E-5	0.0	3.8279233611463865E-6	1.1483770083439159E-5
3	3.521689492254676E-4	9.952600738980605E-5	0.0	7.655846722292773E-6	2.6795463528024706E-5
4	4.4403910989298086E-4	1.760844746127338E-4	0.0	1.9139616805731932E-5	3.445131025031748E-5
5	0.0011407211616216231	2.3350332502992957E-4	0.0	1.9139616805731932E-5	4.5935080333756635E-5
6	0.0011560328550662088	2.602987885579543E-4	0.0	1.9139616805731932E-5	5.359092705604941E-5
7	0.0011713445485107942	2.9857802216941813E-4	3.8279233611463865E-6	2.2967540166878317E-5	6.124677377834218E-5
8	0.0012134517054834045	3.253734856974428E-4	3.8279233611463865E-6	2.2967540166878317E-5	6.507469713948857E-5
9	0.0013933641034572846	3.5982479594776035E-4	3.8279233611463865E-6	3.062338688917109E-5	6.890262050063496E-5
10	0.0025111177249120295	4.1341572300380975E-4	3.8279233611463865E-6	3.062338688917109E-5	7.655846722292773E-5
11	0.002763760666747691	4.7083457342100556E-4	3.8279233611463865E-6	3.062338688917109E-5	7.655846722292773E-5
12	0.0031465530028623296	5.091138070324694E-4	3.8279233611463865E-6	3.8279233611463863E-5	7.655846722292773E-5
13	0.003387712174614552	5.359092705604941E-4	3.8279233611463865E-6	4.210715697261025E-5	8.42143139452205E-5
14	0.003494894028726651	5.74188504171958E-4	3.8279233611463865E-6	4.210715697261025E-5	8.80422373063669E-5
15	0.01475664455721932	5.9332812097769E-4	3.8279233611463865E-6	4.210715697261025E-5	9.569808402865966E-5
16	0.015101157659722494	6.162956611445682E-4	3.8279233611463865E-6	4.210715697261025E-5	1.1100977747324521E-4
17	0.016012203419675335	6.660586648394712E-4	3.8279233611463865E-6	4.5935080333756635E-5	1.2249354755668437E-4
18	0.01683520694232181	7.23477515256667E-4	3.8279233611463865E-6	4.5935080333756635E-5	1.2249354755668437E-4
19	0.0182209151990568	7.502729787846917E-4	3.8279233611463865E-6	4.5935080333756635E-5	1.3014939427897715E-4
20	0.2739032281834685	8.153476759241804E-4	3.8279233611463865E-6	4.5935080333756635E-5	1.416331643624163E-4
21	0.27959535022149323	8.651106796190833E-4	3.8279233611463865E-6	4.9763003694903024E-5	1.4928901108470906E-4
22	0.2803341394301945	8.91906143147108E-4	3.8279233611463865E-6	4.9763003694903024E-5	1.5694485780700184E-4
23	0.28092746755117215	9.187016066751327E-4	3.8279233611463865E-6	4.9763003694903024E-5	1.68428627890441E-4
24	0.28229403619110144	9.646366870088894E-4	3.8279233611463865E-6	5.359092705604941E-5	1.8374032133502654E-4
25	0.28294095523913515	9.837763038146213E-4	3.8279233611463865E-6	5.359092705604941E-5	1.8756824469617293E-4
26	0.2835495950535574	0.0010565068476764026	3.8279233611463865E-6	5.359092705604941E-5	1.952240914184657E-4
27	0.28454102720409435	0.0011062698513713057	3.8279233611463865E-6	5.359092705604941E-5	2.0287993814075848E-4
28	0.3618689070226125	0.0011368932382604768	3.8279233611463865E-6	6.124677377834218E-5	2.0670786150190487E-4
29	0.36426518704669014	0.0011636887017885014	3.8279233611463865E-6	6.507469713948857E-5	2.1436370822419765E-4
30	0.37700834391594645	0.0012019679353999654	3.8279233611463865E-6	6.890262050063496E-5	2.1436370822419765E-4
31	0.8527809384728309	0.0012364192456502828	3.8279233611463865E-6	6.890262050063496E-5	2.1819163158534404E-4
32	0.8566203456040606	0.0012900101727063322	3.8279233611463865E-6	6.890262050063496E-5	2.2201955494649043E-4
33	0.86823809300514	0.0013244614829566498	3.8279233611463865E-6	6.890262050063496E-5	2.2967540166878318E-4
34	0.8690266452175361	0.001355084869845821	3.8279233611463865E-6	7.273054386178134E-5	2.2967540166878318E-4
35	0.8696965318057367	0.0013971920268184312	3.8279233611463865E-6	7.655846722292773E-5	2.3733124839107596E-4
36	0.8704046976275488	0.0014431271071521877	3.8279233611463865E-6	8.42143139452205E-5	2.4115917175222235E-4
37	0.8712544966137233	0.001466094647319066	3.8279233611463865E-6	8.80422373063669E-5	2.4498709511336874E-4
38	0.8720583605195641	0.0014928901108470908	3.8279233611463865E-6	9.187016066751327E-5	2.526429418356615E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	3330	0.0	24.181719	43
GATATCG	2835	0.0	22.659016	36
TGCCGAG	28830	0.0	22.209084	26
GACCGAT	3675	0.0	22.027285	32
CGAGATC	24730	0.0	22.014767	29
ATGCCGA	29215	0.0	21.86467	25
GCCGAGA	29445	0.0	21.827513	27
GAGATCG	25090	0.0	21.690374	30
AGATCGG	25175	0.0	21.590637	31
ACCGATA	3005	0.0	21.448544	33
CGATATC	3090	0.0	20.931128	35
CCGAGAT	26875	0.0	20.379677	28
AATGCCG	33075	0.0	19.319466	24
CCGATAT	3615	0.0	18.134375	34
AAGCAGT	17505	0.0	17.80552	1
ATGCCGT	4560	0.0	17.707708	44
TCGTATG	4700	0.0	17.178303	40
GAGACCG	4980	0.0	16.873377	30
ATCGTAT	3915	0.0	16.857534	39
GATCGGA	39175	0.0	16.804659	1
>>END_MODULE