FastQCFastQC Report
Fri 27 May 2016
SRR522039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23643734
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC84750.014.10325812
CAACGCA89750.013.48892613
GTATCAA92850.013.0623169
ATCAACG97050.012.56488711
AACGCAG101250.012.13060814
GGTATCA103750.011.7958738
CGAGATC46050.011.73772918
AGAGTAC106000.011.31944419
ACGCAGA109600.011.286612515
AGTGGTA110400.011.2645075
TATCAAC110400.011.2445110
AATGCCG50850.011.19100413
GAGATCG49750.010.91043919
AGCGGTT48850.010.86030330
GTGGTAT115050.010.7710326
GCGGTTC53050.010.37432231
GATCGGA52800.010.36578121
TGGTATC120450.010.3063447
CGGTTCA55500.010.3052241
AAGCAGT129900.010.1318271
Produced by FastQC (version 0.11.5)