##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522039_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23643734
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.194157530278424	40.0	39.0	40.0	38.0	40.0
2	39.19780234374147	40.0	39.0	40.0	38.0	40.0
3	39.18967122536567	40.0	39.0	40.0	38.0	40.0
4	39.18537545719301	40.0	39.0	40.0	38.0	40.0
5	39.17718876383908	40.0	39.0	40.0	38.0	40.0
6	39.22999281754735	40.0	39.0	40.0	38.0	40.0
7	39.209428256974974	40.0	39.0	40.0	38.0	40.0
8	39.19329231161203	40.0	39.0	40.0	38.0	40.0
9	39.17222804147602	40.0	39.0	40.0	38.0	40.0
10	39.14824058670259	40.0	39.0	40.0	38.0	40.0
11	39.18260914287058	40.0	39.0	40.0	38.0	40.0
12	39.148256447141556	40.0	39.0	40.0	38.0	40.0
13	39.11809809736482	40.0	39.0	40.0	38.0	40.0
14	39.09160782302829	40.0	39.0	40.0	38.0	40.0
15	39.06120911358587	40.0	39.0	40.0	38.0	40.0
16	39.00656520666321	40.0	39.0	40.0	38.0	40.0
17	38.95648373476034	40.0	39.0	40.0	38.0	40.0
18	38.90544767590432	40.0	39.0	40.0	37.0	40.0
19	38.857407252170916	40.0	39.0	40.0	37.0	40.0
20	38.80004566960532	40.0	39.0	40.0	37.0	40.0
21	38.684959194685575	40.0	39.0	40.0	36.0	40.0
22	38.601235405541274	40.0	39.0	40.0	36.0	40.0
23	38.52114936667787	40.0	39.0	40.0	36.0	40.0
24	38.44126452276954	40.0	38.0	40.0	35.0	40.0
25	38.642453472027725	40.0	39.0	40.0	36.0	40.0
26	38.55351037192349	40.0	39.0	40.0	36.0	40.0
27	38.48315384532748	40.0	39.0	40.0	36.0	40.0
28	38.53690559198475	40.0	39.0	40.0	36.0	40.0
29	38.47897798207339	40.0	39.0	40.0	36.0	40.0
30	38.41319129203534	40.0	39.0	40.0	36.0	40.0
31	38.33261945850008	40.0	39.0	40.0	36.0	40.0
32	38.25663069970251	40.0	39.0	40.0	36.0	40.0
33	38.194918577581696	40.0	39.0	40.0	36.0	40.0
34	38.13584305253984	40.0	39.0	40.0	35.0	40.0
35	38.05213533530702	40.0	39.0	40.0	35.0	40.0
36	37.8939590506305	40.0	39.0	40.0	35.0	40.0
37	37.816313912176476	40.0	39.0	40.0	35.0	40.0
38	37.74543048065082	40.0	39.0	40.0	35.0	40.0
39	37.675737258759554	40.0	38.0	40.0	35.0	40.0
40	37.5725775801741	40.0	38.0	40.0	34.0	40.0
41	37.37928370366542	40.0	38.0	40.0	34.0	40.0
42	37.285536709218604	40.0	38.0	40.0	33.0	40.0
43	37.19711827243531	40.0	38.0	40.0	33.0	40.0
44	37.10246499135881	40.0	38.0	40.0	33.0	40.0
45	36.973763661864915	40.0	38.0	40.0	33.0	40.0
46	36.75400285758586	40.0	38.0	40.0	33.0	40.0
47	36.64179490430742	39.0	38.0	40.0	32.0	40.0
48	36.53444747771228	39.0	37.0	40.0	32.0	40.0
49	36.43691990444487	39.0	37.0	40.0	32.0	40.0
50	36.31910166981239	39.0	37.0	40.0	31.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1538820239817511
1	2	-0.1679159224558262
1	3	-0.151813977667544
1	4	-0.16127474134789566
1	5	-0.1673329517487332
1	6	-0.1595849239107423
1	7	-0.1629080256354456
1	8	-0.1745296782185335
1	9	-0.1791253804197268
1	10	-0.17213833270277235
1	11	-0.18334029692438492
1	12	-0.18108032587682032
1	13	-0.16903870201251436
1	14	-0.17812149553109435
1	15	-0.17063825376133934
1	16	-0.19469671918542986
1	17	-0.1884361630681326
1	18	-0.19168281545938015
1	19	-0.1765791237815435
1	20	-0.17615240129666176
1	21	-0.17207209078542007
1	22	-0.20072181223982
1	23	-0.1644475548215567
1	24	-0.17566927181078285
1	25	-0.22061937439799095
1	26	-0.24472382409756932
1	27	-0.2065359593373799
1	28	-0.183830031921417
1	29	-0.1280088178356067
1	30	-0.1562008676371036
1	31	-0.1318328188456661
1	32	-0.09377398212458843
1	33	-0.1145948547746869
1	34	-0.12112434446324727
1	35	-0.15419155484211444
1	36	-0.08967130648266419
1	37	-0.10920857400086703
1	38	-0.1339534919717238
1	39	-0.11422028899671233
1	40	-0.14461734011517535
1	41	-0.11712641613091535
1	42	-0.09537205636527091
1	43	-0.12167364000494985
1	44	-0.11579341881441962
1	45	-0.13490206024919615
1	46	-0.0833630363399962
1	47	-0.1183054358772253
1	48	-0.11778751803709042
1	49	-0.13563168711770857
1	50	-0.14277582488581686
2	1	-0.15830428629256943
2	2	-0.1454046645528564
2	3	-0.1452709721884773
2	4	-0.1668043772207497
2	5	-0.15612677285333376
2	6	-0.1602389313630539
2	7	-0.1576869599093058
2	8	-0.14710372371173008
2	9	-0.1470250340099284
2	10	-0.17930906809782243
2	11	-0.11701303271372865
2	12	-0.14333713682333382
2	13	-0.15516308477676688
2	14	-0.14973159339987063
2	15	-0.14449504729729767
2	16	-0.1513653537475932
2	17	-0.14571493058070928
2	18	-0.17495307596768583
2	19	-0.15986001883126733
2	20	-0.1628879726046648
2	21	-0.16268596817246106
2	22	-0.16962339235524837
2	23	-0.19912289765693458
2	24	-0.2391142720962378
2	25	-0.3032551065200195
2	26	-0.2877579277130806
2	27	-0.2948248429827416
2	28	-0.263545790730646
2	29	-0.2386220070656293
2	30	-0.2311720712753882
2	31	-0.16302023423256173
2	32	-0.1976438165055825
2	33	-0.21186223355809375
2	34	-0.1857176393972395
2	35	-0.1986535955034796
2	36	-0.20008486504457323
2	37	-0.19975674182547465
2	38	-0.17453266728919914
2	39	-0.214618467788263
2	40	-0.2377975400948671
2	41	-0.1591799858309102
2	42	-0.1860262888610933
2	43	-0.20270500500707556
2	44	-0.20466915620813353
2	45	-0.20931531542909454
2	46	-0.27089180968079063
2	47	-0.25056915893875953
2	48	-0.2732103076504657
2	49	-0.2755779848172253
2	50	-0.2758427197223554
3	1	-0.1507846781778852
3	2	-0.15459870732849623
3	3	-0.1508330145887058
3	4	-0.15471835459042182
3	5	-0.17987260546237138
3	6	-0.15344778230716116
3	7	-0.15604004515725478
3	8	-0.15993005797873394
3	9	-0.18051233214095674
3	10	-0.1668915173328145
3	11	-0.16304461912680068
3	12	-0.17775210427834764
3	13	-0.17289181539002385
3	14	-0.17657799233075622
3	15	-0.17860117853867052
3	16	-0.17682064944952458
3	17	-0.17482790913383184
3	18	-0.2083512699342478
3	19	-0.19718585843178005
3	20	-0.212068469490589
3	21	-0.20733624721919597
3	22	-0.20347633211705585
3	23	-0.2508032638998543
3	24	-0.2688978532490154
3	25	-0.286092229695285
3	26	-0.2826034741052439
3	27	-0.25493832481863166
3	28	-0.24334843468500367
3	29	-0.24391412449036665
3	30	-0.25117646281617567
3	31	-0.17988971911182006
3	32	-0.22289660514872622
3	33	-0.2020803445073156
3	34	-0.22266297154998682
3	35	-0.22565949194736845
3	36	-0.25050948563221453
3	37	-0.2709565080011487
3	38	-0.2916840197031192
3	39	-0.24095607543717534
3	40	-0.24278336951569202
3	41	-0.22473375479150803
3	42	-0.2311201794632467
3	43	-0.23208204462873283
3	44	-0.22475848555267675
3	45	-0.29219470637158196
3	46	-0.2583360468935254
3	47	-0.23079953539682663
3	48	-0.28731239895862615
3	49	-0.23641521114583242
3	50	-0.2680808804545194
4	1	-0.11171163389794714
4	2	-0.11812460406849112
4	3	-0.12051064810800938
4	4	-0.1065827319329884
4	5	-0.12032045214881038
4	6	-0.11037856876446028
4	7	-0.1129907668863055
4	8	-0.11326189794638708
4	9	-0.12394466185717334
4	10	-0.10025477175494046
4	11	-0.11847923079016454
4	12	-0.12294494511433385
4	13	-0.11954595005603608
4	14	-0.1257779034509383
4	15	-0.09880502180094197
4	16	-0.14502006144274304
4	17	-0.12507813635751575
4	18	-0.1371507452839893
4	19	-0.13457198155287386
4	20	-0.13383523037568068
4	21	-0.119226471429009
4	22	-0.12621205475967656
4	23	-0.14841293109466136
4	24	-0.15718600631699786
4	25	-0.1983544829503998
4	26	-0.1954047402339114
4	27	-0.1942873654457813
4	28	-0.14554289172994572
4	29	-0.12581185142996532
4	30	-0.15442694766964138
4	31	-0.1473788246970642
4	32	-0.1614965520125864
4	33	-0.16467549068117648
4	34	-0.13867990456213164
4	35	-0.1358180593266809
4	36	-0.11425521041908127
4	37	-0.13546321473359058
4	38	-0.12495756386594792
4	39	-0.12059393766682547
4	40	-0.1449184317423189
4	41	-0.11286076633341224
4	42	-0.12105934177682798
4	43	-0.13656982614319446
4	44	-0.1519716702297771
4	45	-0.12468885823093956
4	46	-0.13419252224775846
4	47	-0.15465494427931503
4	48	-0.17731231199628894
4	49	-0.12850538543987255
4	50	-0.19935971592244073
5	1	-0.10823298536625003
5	2	-0.11199801410329968
5	3	-0.11776263740409121
5	4	-0.10529028456127776
5	5	-0.11045372921542906
5	6	-0.11851095447404703
5	7	-0.11396138485319085
5	8	-0.12082896695663692
5	9	-0.11833070697259274
5	10	-0.12832168806121302
5	11	-0.13799585860104457
5	12	-0.1513442201114188
5	13	-0.14793762817132716
5	14	-0.15614377772308075
5	15	-0.14709495862804545
5	16	-0.1530353816672232
5	17	-0.14972694068417525
5	18	-0.15753885234092024
5	19	-0.18146213328532212
5	20	-0.192269901602657
5	21	-0.1533410530191901
5	22	-0.19847737515618746
5	23	-0.20894204268973482
5	24	-0.16952341271543503
5	25	-0.2524273476112029
5	26	-0.24935001794338518
5	27	-0.24875314098895984
5	28	-0.20234295484466713
5	29	-0.2078951073738935
5	30	-0.21657611092257412
5	31	-0.22895645596133107
5	32	-0.22179622839402668
5	33	-0.2519900853209478
5	34	-0.23093527965119165
5	35	-0.21447315491930397
5	36	-0.19552518556542964
5	37	-0.1731933685817424
5	38	-0.22389666616678738
5	39	-0.18367784580872382
5	40	-0.24705448409882536
5	41	-0.2055591846868623
5	42	-0.18047531665155248
5	43	-0.23624245957728363
5	44	-0.2403560254671362
5	45	-0.24973245330654947
5	46	-0.1892684489953922
5	47	-0.2123334931311618
5	48	-0.2376677196968089
5	49	-0.26218053179577794
5	50	-0.19674175538938243
6	1	-0.1113602894735024
6	2	-0.12742716238403062
6	3	-0.1373573650841351
6	4	-0.1289596762644507
6	5	-0.12146510376560826
6	6	-0.11786475781527628
6	7	-0.1371262391546324
6	8	-0.14179895356244998
6	9	-0.14329112755862639
6	10	-0.14290113068163635
6	11	-0.1306717358866294
6	12	-0.14068356873482202
6	13	-0.1784634092259978
6	14	-0.15357092989140853
6	15	-0.1461149157420394
6	16	-0.12934063488074088
6	17	-0.17135427740895182
6	18	-0.1715325399822163
6	19	-0.18347357971026668
6	20	-0.18117035046159913
6	21	-0.19207288730182626
6	22	-0.20096683381748193
6	23	-0.233186492045526
6	24	-0.24050442605560818
6	25	-0.28118121390443207
6	26	-0.2519566355332614
6	27	-0.25941851037440244
6	28	-0.2313783111508556
6	29	-0.25017408112339723
6	30	-0.24550307598288157
6	31	-0.17699825402143432
6	32	-0.2001610679849577
6	33	-0.20531118258392667
6	34	-0.2549431681619936
6	35	-0.2046013094064989
6	36	-0.19216801387922544
6	37	-0.17768250343571879
6	38	-0.20219155864872107
6	39	-0.2186189136737653
6	40	-0.24501895976488441
6	41	-0.19864541966736482
6	42	-0.22051527094790657
6	43	-0.23616423859987634
6	44	-0.2129584488879459
6	45	-0.2168900347110707
6	46	-0.24999890290643378
6	47	-0.23677857982946904
6	48	-0.22481559782894323
6	49	-0.24189020421250262
6	50	-0.3381833137032402
7	1	-0.11407674906176624
7	2	-0.10351613562495032
7	3	-0.10515877198432122
7	4	-0.11434320331625258
7	5	-0.11484648938606767
7	6	-0.10432184905828734
7	7	-0.10422875119022024
7	8	-0.09878102256035248
7	9	-0.11400094601149391
7	10	-0.11350700009677439
7	11	-0.10053290705122464
7	12	-0.10665783013669738
7	13	-0.14107127945420217
7	14	-0.12305141125890628
7	15	-0.10364147472519392
7	16	-0.11510625188577706
7	17	-0.09309395449068347
7	18	-0.12639568460360806
7	19	-0.10387756007010296
7	20	-0.1362017950555554
7	21	-0.1524743223907521
7	22	-0.1691259956760689
7	23	-0.19162099703849123
7	24	-0.17795049819840614
7	25	-0.2210571877125176
7	26	-0.23611062461645815
7	27	-0.2237252822450131
7	28	-0.21345581244049328
7	29	-0.19709473461278293
7	30	-0.21116711340810213
7	31	-0.19523841541456477
7	32	-0.2023487305861238
7	33	-0.23265232871348474
7	34	-0.18383053985571252
7	35	-0.19417279586042469
7	36	-0.21720281559359478
7	37	-0.22348535101420453
7	38	-0.21932404186001264
7	39	-0.20550788377929763
7	40	-0.2134453967649108
7	41	-0.24650282863452588
7	42	-0.23584960012906464
7	43	-0.24169767773733497
7	44	-0.2402886127311632
7	45	-0.26100717867556256
7	46	-0.321550786507558
7	47	-0.28473840107761816
7	48	-0.34636877652426534
7	49	-0.35857285241261394
7	50	-0.2994468793630318
8	1	-0.10462670731887869
8	2	-0.10395258026132126
8	3	-0.09712448256077977
8	4	-0.08686286440386937
8	5	-0.08255554039654811
8	6	-0.1109979858073018
8	7	-0.10774753225663858
8	8	-0.11287565808635236
8	9	-0.0957181884046534
8	10	-0.106609777699326
8	11	-0.10308639247632101
8	12	-0.09794530103692978
8	13	-0.12903621910032115
8	14	-0.12745018103251482
8	15	-0.12906716501807125
8	16	-0.11725170665808093
8	17	-0.1256181491439179
8	18	-0.17126345227612205
8	19	-0.17842434281680397
8	20	-0.1386139700559994
8	21	-0.169865877595619
8	22	-0.15925722539120102
8	23	-0.15506334481398198
8	24	-0.17544887357325223
8	25	-0.19255217053245843
8	26	-0.24228778240873083
8	27	-0.2420163861549156
8	28	-0.2412133135981307
8	29	-0.25363181179902483
8	30	-0.2377172883198071
8	31	-0.15878512243937593
8	32	-0.1701842555029529
8	33	-0.18510142564674226
8	34	-0.18908646073935387
8	35	-0.15840288031006367
8	36	-0.1395491530059303
8	37	-0.2066061166665989
8	38	-0.1359354536988704
8	39	-0.20209249533439078
8	40	-0.19472105271163542
8	41	-0.1968881061685579
8	42	-0.21132053022759578
8	43	-0.1784226605447614
8	44	-0.16755060179546888
8	45	-0.20888287518166493
8	46	-0.19451186589711966
8	47	-0.1878110574206886
8	48	-0.19417606827376943
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113	2	-0.04403677548665996
113	3	-0.08687628561974492
113	4	-0.05317857523071012
113	5	-0.059608791779851344
113	6	-0.04621770855622742
113	7	-0.04128089930806311
113	8	-0.03605934455250548
113	9	-0.043960493191427474
113	10	-0.028132021661789963
113	11	-0.021964113454806977
113	12	-0.026997996738977292
113	13	-0.010591237790485764
113	14	0.0039159153912109446
113	15	-0.030231743131437838
113	16	0.00963422838432848
113	17	0.01457031114161822
113	18	0.0028521255811426727
113	19	0.018680670491946216
113	20	0.02835565107969984
113	21	0.03410585984065051
113	22	0.04493929927845386
113	23	0.031104358400391163
113	24	0.04003687677376888
113	25	0.04117482804784345
113	26	0.03569608377350875
113	27	0.047463719297113016
113	28	0.07921002359707785
113	29	0.09951546110831089
113	30	0.10130655706777247
113	31	0.11906808741748165
113	32	0.14094745943252462
113	33	0.150321593779843
113	34	0.15277368906384226
113	35	0.17062112258241058
113	36	0.20288420089834602
113	37	0.22110114116708246
113	38	0.22973493351084784
113	39	0.24560977835569986
113	40	0.22918771637645108
113	41	0.30203721556318186
113	42	0.31538178621621427
113	43	0.3227649798291736
113	44	0.31672906422126346
113	45	0.3652672877063168
113	46	0.4023518439011582
113	47	0.4216807490395098
113	48	0.4722702044089573
113	49	0.47708559016970753
113	50	0.5348601168578142
114	1	-0.005480755109438462
114	2	-0.006723049396597958
114	3	-0.016044539742424035
114	4	-0.01366351556991674
114	5	-0.007397764038813648
114	6	-0.003743485199883878
114	7	0.01451309504417253
114	8	0.012318885045608852
114	9	5.474283803579283E-4
114	10	0.0032027987654998924
114	11	0.0015959633866202694
114	12	2.577980534610447E-4
114	13	7.215707579177888E-4
114	14	-0.004246506330979116
114	15	-0.003998830571333656
114	16	0.014852668982818784
114	17	0.02238882259210584
114	18	-0.0076939662557435895
114	19	0.021462944874144796
114	20	0.014556485785448103
114	21	0.04836671593375286
114	22	0.049020763499108
114	23	0.07008304953095035
114	24	0.08331636549923616
114	25	0.03557881202563351
114	26	0.035866487082031995
114	27	0.02823809886862705
114	28	0.07511317411692175
114	29	0.10993391673102337
114	30	0.09629196957419595
114	31	0.11500537180453563
114	32	0.09284461901212637
114	33	0.11869310428232893
114	34	0.12998208344891538
114	35	0.1602385996798148
114	36	0.1567022440136867
114	37	0.16664669683179056
114	38	0.1791146364581664
114	39	0.22742009451106782
114	40	0.2676220074786073
114	41	0.2818469539320603
114	42	0.32257306806295816
114	43	0.353183810424035
114	44	0.353724387013699
114	45	0.33940533788531724
114	46	0.3916951352787805
114	47	0.4183280989396465
114	48	0.42596583051712855
114	49	0.4644236862827995
114	50	0.49076273278897276
116	1	-0.02474080132203227
116	2	-0.02315741716101627
116	3	-0.017824792975687842
116	4	-0.022405920128584
116	5	-0.014300135410302062
116	6	-0.027150057250807436
116	7	-0.022101950908300694
116	8	-0.010633731687427428
116	9	-0.033407885380107416
116	10	-0.03337178712128264
116	11	-0.010758122588747199
116	12	-0.005234449013983067
116	13	-9.285214511081108E-4
116	14	0.011864449814218858
116	15	-0.01146283357564215
116	16	0.0019909912118691864
116	17	0.02399815480141143
116	18	0.012498233535893633
116	19	0.009185983313379609
116	20	0.024036872841001866
116	21	0.035986732206723104
116	22	0.05178868913367296
116	23	0.05890883161150384
116	24	0.086201161977975
116	25	0.025199026255066315
116	26	0.02102969204463534
116	27	0.03170025800265819
116	28	0.1014481778759233
116	29	0.08086738277999217
116	30	0.10271783886315689
116	31	0.17277396815363488
116	32	0.16094136076675625
116	33	0.15686890347463844
116	34	0.18574452453127321
116	35	0.20004075492297346
116	36	0.23847335029344663
116	37	0.2686292090233309
116	38	0.25708792640241995
116	39	0.2731209901657792
116	40	0.2905089179750888
116	41	0.33286098432861877
116	42	0.33586063146409373
116	43	0.36346321137925486
116	44	0.3600508850574542
116	45	0.4040037008867756
116	46	0.5164405817890128
116	47	0.5187538914868597
116	48	0.5389138996297902
116	49	0.5170678581044541
116	50	0.5440011876819497
117	1	-0.06228033979108005
117	2	-0.05602040407659814
117	3	-0.06310962597685688
117	4	-0.051320363330397356
117	5	-0.058078634465388745
117	6	-0.06398848016208802
117	7	-0.03982713400060334
117	8	-0.0579804744645358
117	9	-0.06343797585005717
117	10	-0.05110108745855513
117	11	-0.02568107294628419
117	12	-0.03098974072140237
117	13	-0.06101164423333216
117	14	-0.05423285313400328
117	15	-0.027238863260166113
117	16	-0.010859833557333332
117	17	-0.029494422338856907
117	18	-0.03743773150625884
117	19	-0.010987695261526653
117	20	-0.016343165957927397
117	21	-0.00325784203128876
117	22	0.018961370090359253
117	23	0.02180816623297943
117	24	0.009716351011391566
117	25	-0.001974933000404633
117	26	-6.276552868413887E-4
117	27	-5.629089732295256E-4
117	28	0.04005127151358323
117	29	0.060911560167667744
117	30	0.09529913299055437
117	31	0.11098788856704545
117	32	0.1282927472429023
117	33	0.15142187787289885
117	34	0.11782364821554836
117	35	0.13341255927262807
117	36	0.19708072554040967
117	37	0.1629542662467358
117	38	0.20012541900348424
117	39	0.16877710956555347
117	40	0.23551224452188535
117	41	0.2690681221098217
117	42	0.293982257240458
117	43	0.2983860006272039
117	44	0.3417694222979577
117	45	0.3050546642834533
117	46	0.3843224581933029
117	47	0.3209042813456193
117	48	0.41922731717379946
117	49	0.44912026038060304
117	50	0.457818877457548
118	1	-0.028117378867555942
118	2	-0.04215602720459799
118	3	-0.025698185546573882
118	4	-0.04594697052399965
118	5	-0.027771337674259655
118	6	-0.03542387182865525
118	7	-0.02393411175960125
118	8	-0.019997497654216545
118	9	-0.027263077414033887
118	10	-0.02786757427223563
118	11	-0.023170055980060056
118	12	-0.021587477799037913
118	13	-0.018298268101709425
118	14	-0.026600523919157126
118	15	-0.01012990578279016
118	16	0.002548163054875374
118	17	0.01151432278853548
118	18	-0.016245544152951652
118	19	0.017830791380589517
118	20	-0.006142069940210604
118	21	-0.006758264449750584
118	22	0.002978388027962353
118	23	-0.013170636793368828
118	24	0.027222161237745013
118	25	-0.031068137599163492
118	26	-0.05416822434350621
118	27	-0.0286300921756677
118	28	0.03547851469308938
118	29	0.04454456241882099
118	30	0.0671306626744439
118	31	0.07011298698218127
118	32	0.12421210325007337
118	33	0.12653755940122124
118	34	0.1232614793014406
118	35	0.15091449468968676
118	36	0.19573097085358881
118	37	0.2046324619768356
118	38	0.21422466553416797
118	39	0.23773103560947817
118	40	0.26746077401247703
118	41	0.33246303391261733
118	42	0.3470410248790827
118	43	0.36822459181175304
118	44	0.3712543520483038
118	45	0.3755151442922582
118	46	0.42409673324519304
118	47	0.42245263747604156
118	48	0.4415720896428752
118	49	0.4113059040282323
118	50	0.48055322686786894
119	1	-0.048757299020891764
119	2	-0.035312907654720505
119	3	-0.04210297688174336
119	4	-0.036093193143969415
119	5	-0.042771355614107165
119	6	-0.04174556766391646
119	7	-0.05342974194486061
119	8	-0.06139543499467237
119	9	-0.06167334732789698
119	10	-0.0462473479734129
119	11	-0.06899934392443896
119	12	-0.06609866187064739
119	13	-0.05085857829837437
119	14	-0.04645832439413056
119	15	-0.04463862631418891
119	16	-0.03139522782613824
119	17	-0.029927423139703535
119	18	-0.013160574036469086
119	19	-0.03486999206302244
119	20	-0.020471427691056476
119	21	-0.04124808754852438
119	22	-0.025032641413297085
119	23	0.029308548783227195
119	24	-0.017711455550184496
119	25	-0.06746686959732529
119	26	-0.028240487847746465
119	27	-0.022048735444187173
119	28	0.05803796461155741
119	29	0.07646234202579905
119	30	0.05402594279389206
119	31	0.09198325675085783
119	32	0.11350452826148683
119	33	0.1190189559694943
119	34	0.13012818488161315
119	35	0.1466756944611447
119	36	0.19309738951741195
119	37	0.16060718946589958
119	38	0.16792731448734344
119	39	0.1936923429810662
119	40	0.2201727719459683
119	41	0.2722820736712066
119	42	0.3122210053029164
119	43	0.33611865838306443
119	44	0.28534459688087566
119	45	0.3466228750392375
119	46	0.3732595978399331
119	47	0.3925726515502532
119	48	0.3933512513366253
119	49	0.44172608395284385
119	50	0.41992411814394615
120	1	-0.04848111102265307
120	2	-0.052468270804972406
120	3	-0.04276986187257137
120	4	-0.04445077837686995
120	5	-0.03913000133462674
120	6	-0.0547639534560318
120	7	-0.0572657658251714
120	8	-0.0691966257185399
120	9	-0.03212637074476277
120	10	-0.09629809222554542
120	11	-0.06959634284793026
120	12	-0.060802654190368344
120	13	-0.07046418196227933
120	14	-0.06212285661541017
120	15	-0.066783189295613
120	16	-0.0538500568110436
120	17	-0.055380403403120226
120	18	-0.05108656661322897
120	19	-0.06573482523974405
120	20	-0.03766375880559991
120	21	2.6999089399737386E-4
120	22	-0.022488225461238187
120	23	-0.008895163959898866
120	24	0.022033365572170283
120	25	-0.07803049674319595
120	26	-0.03269665187617932
120	27	-0.008331024626002659
120	28	0.03876683990973362
120	29	0.052634396511692216
120	30	0.07236221962399014
120	31	0.15558328304850733
120	32	0.1509864017280904
120	33	0.20026705306616321
120	34	0.19214615354977838
120	35	0.21395108250892747
120	36	0.270285278324053
120	37	0.27724329163177686
120	38	0.3099768720733138
120	39	0.2728983734615582
120	40	0.3092897651393116
120	41	0.4076958718698336
120	42	0.36452558649244793
120	43	0.44288408680415614
120	44	0.4390519592945097
120	45	0.5006737056570643
120	46	0.5325583962018214
120	47	0.5585413539207309
120	48	0.5962942053769424
120	49	0.5904236974569912
120	50	0.6374976103658554
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11034.0
3	527.0
4	762.0
5	773.0
6	1524.0
7	2656.0
8	3221.0
9	3584.0
10	4972.0
11	7955.0
12	6995.0
13	9568.0
14	13632.0
15	17413.0
16	17978.0
17	19885.0
18	26547.0
19	35022.0
20	50698.0
21	35117.0
22	49587.0
23	72701.0
24	83842.0
25	61301.0
26	82859.0
27	108342.0
28	124303.0
29	117207.0
30	124269.0
31	151621.0
32	175289.0
33	218985.0
34	310520.0
35	460312.0
36	738396.0
37	1417555.0
38	3287831.0
39	1.5376274E7
40	412677.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.4584271261042	26.469057811827106	22.84731749097538	29.225197571093307
2	26.052247077386337	27.908070696447524	23.898581332373304	22.141100893792835
3	24.499844229342116	25.46065270401029	24.768909174836768	25.270593891810826
4	29.192825464877924	23.836877880625792	22.028271845724536	24.942024808771745
5	27.620012175745906	26.54901346767426	23.62552755009934	22.205446806480502
6	27.822369922511204	24.73017309152773	20.96916317073538	26.478293815225683
7	25.782074942058575	24.27503116474211	24.637000646176197	25.30589324702312
8	26.15089588433434	23.85575492668607	24.460963926466263	25.532385262513323
9	25.439777624896724	23.734178312709194	24.368031269616786	26.458012792777293
10	25.60715518902534	25.463772433663955	22.678101082960424	26.25097129435028
11	26.159574465835394	24.806926328562433	23.428508107552563	25.604991098049606
12	26.060824402778344	24.238586849268394	24.183240261457854	25.517348486495408
13	25.7640523277753	24.520733484820965	24.16616597023127	25.549048217172466
14	25.389745968212974	24.93511388683361	24.288642394640373	25.38649775031304
15	25.096556237690713	24.566978295391078	24.44887512268578	25.887590344232432
16	25.38460871508928	24.50753382348494	24.2342137156592	25.87364374576658
17	25.383520217238104	24.60506449615784	24.455151627065337	25.55626365953872
18	25.169040558483697	24.37148040998939	24.3887238792316	26.070755152295316
19	25.462790201451085	24.432862785252986	24.274110170127734	25.83023684316819
20	25.42176416751472	24.443908517767373	24.512452054734844	25.621875259983064
21	25.2956745326267	24.296767168840592	24.438542575381707	25.969015723151006
22	25.56838269475209	24.304502207855315	24.22044116672411	25.906673930668482
23	25.409662450102	24.473152167927452	24.582090121636455	25.53509526033409
24	25.523573888835717	24.219051053796537	24.396017156673558	25.861357900694195
25	25.72743051024458	24.15483792044066	24.197459434584754	25.920272134730006
26	25.664752882699933	24.427545206690066	24.416043636761724	25.491658273848277
27	25.51955490965253	24.106780017203803	24.519993699300514	25.853671373843152
28	25.753677596132725	24.180144059290246	24.264058682603462	25.802119661973567
29	25.636281531152154	24.373706525502133	24.512904484552795	25.47710745879292
30	25.632767006349415	24.100179765314177	24.434224346045415	25.832828882290986
31	25.801051088216308	24.138631373089904	24.238245194320214	25.82207234437357
32	25.68178134722585	24.35063733010059	24.375130312238504	25.592451010435063
33	25.617966056819085	24.096366861711985	24.34901003468453	25.9366570467844
34	25.797668850818194	24.163672675321433	24.15529794347594	25.883360530384437
35	25.619357442812312	24.323765817969356	24.4566165957056	25.60026014351273
36	25.621749453663075	24.122664743609274	24.354861380578193	25.900724422149462
37	25.815367807859204	24.205359485161416	24.110577089110073	25.868695617869314
38	25.70415555267438	24.43574374356742	24.300792790228364	25.55930791352984
39	25.69935205890323	24.126155636666226	24.290206165619402	25.884286138811145
40	25.890728175868865	24.195708307771195	24.041254493086267	25.872309023273672
41	25.73671465373914	24.407566269263587	24.26220623392806	25.59351284306921
42	25.763931854082077	24.105034613631304	24.220356843745694	25.910676688540928
43	25.93401267594767	24.184335220251622	23.986738791943328	25.894913311857387
44	25.765234275057463	24.454649905700904	24.16277740866493	25.6173384105767
45	25.722054080485922	24.150881167676303	24.210216658543	25.91684809329477
46	25.892234958122778	24.164361841541098	23.991874942479264	25.95152825785686
47	25.810336711876523	24.31193659092501	24.18793413642069	25.689792560777775
48	25.710410051587644	24.02432340546008	24.13436278913769	26.13090375381459
49	25.85490361599308	24.074486221481557	23.94752328930274	26.12308687322263
50	25.761877864778832	24.230878098608017	24.196453010731105	25.810791025882047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3013.0
1	1830.0
2	647.0
3	520.5
4	394.0
5	366.0
6	338.0
7	362.5
8	387.0
9	497.5
10	608.0
11	1005.0
12	1402.0
13	1820.0
14	2238.0
15	3703.0
16	5168.0
17	8048.5
18	10929.0
19	15890.5
20	20852.0
21	30842.5
22	40833.0
23	57972.0
24	75111.0
25	93693.0
26	112275.0
27	140046.0
28	167817.0
29	197073.0
30	226329.0
31	271009.5
32	315690.0
33	372299.5
34	428909.0
35	497376.5
36	565844.0
37	640848.0
38	715852.0
39	803346.5
40	890841.0
41	978286.0
42	1065731.0
43	1152402.5
44	1239074.0
45	1345008.0
46	1450942.0
47	1552370.5
48	1653799.0
49	1748881.0
50	1843963.0
51	1903941.0
52	1963919.0
53	1972080.0
54	1980241.0
55	1935214.0
56	1890187.0
57	1810690.0
58	1731193.0
59	1625273.0
60	1519353.0
61	1355658.0
62	1191963.0
63	1028797.5
64	865632.0
65	728206.0
66	590780.0
67	497900.5
68	405021.0
69	331774.5
70	258528.0
71	208057.5
72	157587.0
73	129654.0
74	101721.0
75	82637.0
76	63553.0
77	51546.5
78	39540.0
79	31642.5
80	23745.0
81	17570.5
82	11396.0
83	8490.0
84	5584.0
85	4050.0
86	2516.0
87	1815.0
88	1114.0
89	780.0
90	446.0
91	333.0
92	220.0
93	152.5
94	85.0
95	65.0
96	45.0
97	48.0
98	51.0
99	189.5
100	328.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5029281753888789
2	0.0
3	0.0
4	0.0
5	3.3835603124278083E-5
6	2.4530812265101615E-4
7	1.184246109349733E-4
8	4.2294503905347604E-6
9	3.383560312427809E-4
10	1.0996571015390378E-4
11	1.6494856523085566E-4
12	0.0
13	0.0
14	0.0
15	0.0
16	1.2265406132550808E-4
17	0.0
18	0.0
19	1.2265406132550808E-4
20	0.0015522082933262572
21	0.0
22	8.50119528497487E-4
23	0.0
24	9.431674370892516E-4
25	0.013073231157142946
26	0.014731175710232573
27	0.03494794857698873
28	0.04813537489467611
29	0.06561569335875628
30	0.08054142378695345
31	0.12494219398678738
32	0.15637123983885118
33	0.1611801249328892
34	0.1565235000529104
35	0.16199640885826241
36	0.19117538710256174
37	0.1878510390956014
38	0.21137524216775574
39	0.2157146582684444
40	0.2290585742505816
41	0.251800329000487
42	0.26215825300690665
43	0.28719236986848184
44	0.2862745791337358
45	0.30740068383445696
46	0.3220599588880504
47	0.3267884844246683
48	0.32831531601565134
49	0.3257226629262535
50	0.3363428128568863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.3643734E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.0449815205266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69765666168895	40.43773105145822
2	10.963954958823988	11.412376664477609
3	4.022178339545408	6.280025920617094
4	2.0739953624416017	4.317642012477242
5	1.2063411419978443	3.1392001221364376
6	0.7668653380503301	2.394689540853709
7	0.5690934818911946	2.0732921823935553
8	0.42042307956482083	1.750472912540319
9	0.31581018760029544	1.4792701839886273
>10	1.7809701880029327	16.904884003346258
>50	0.12507452237098324	4.469861284860387
>100	0.05679691170194439	4.970162338863928
>500	7.744541984859378E-4	0.25931406957844155
>1k	4.910340906859904E-5	0.03558551047818419
>5k	8.134356063420986E-6	0.029046208918856058
>10k+	8.13435606342034E-6	0.046445993011039854
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2688351171604283E-4	2.5376702343208564E-5	0.0	0.0	8.458900781069521E-6
2	1.3534241249711233E-4	6.344175585802141E-5	0.0	0.0	1.6917801562139042E-5
3	1.4803076366871662E-4	9.727735898229949E-5	0.0	4.2294503905347604E-6	2.9606152733743324E-5
4	1.5648966444978615E-4	1.2688351171604283E-4	0.0	4.2294503905347604E-6	3.3835603124278083E-5
5	1.9878416835513376E-4	1.607191148403209E-4	0.0	4.2294503905347604E-6	5.075340468641713E-5
6	2.0301361874566851E-4	1.9455471796459898E-4	0.0	4.2294503905347604E-6	7.190065663909093E-5
7	2.157019699172728E-4	2.0724306913620327E-4	0.0	1.2688351171604282E-5	8.035955742016045E-5
8	2.241608706983423E-4	2.2839032108887709E-4	0.0	1.2688351171604282E-5	9.304790859176474E-5
9	2.7491427538475945E-4	2.6222592421315516E-4	0.0	1.2688351171604282E-5	1.0573625976336902E-4
10	4.440922910061499E-4	2.9606152733743324E-4	0.0	1.2688351171604282E-5	1.184246109349733E-4
11	5.286812988168451E-4	3.1720877929010703E-4	0.0	1.2688351171604282E-5	1.3957186288764711E-4
12	6.132703066275403E-4	3.425854816333156E-4	0.0	1.2688351171604282E-5	1.607191148403209E-4
13	6.851709632666312E-4	3.6373273358598945E-4	0.0	1.2688351171604282E-5	1.9032526757406423E-4
14	8.67037330059626E-4	3.8487998553866324E-4	0.0	1.6917801562139042E-5	2.157019699172728E-4
15	0.0033666425108656696	3.975683367102675E-4	0.0	1.6917801562139042E-5	2.2839032108887709E-4
16	0.0037768991987475412	4.187155886629413E-4	0.0	1.6917801562139042E-5	2.6222592421315516E-4
17	0.00460164202490182	4.398628406156151E-4	0.0	1.6917801562139042E-5	2.8337317616582895E-4
18	0.005494056057304654	4.7792789413042796E-4	0.0	1.6917801562139042E-5	3.00290977727968E-4
19	0.006593713158843692	5.075340468641713E-4	0.0	1.6917801562139042E-5	3.298971304617113E-4
20	0.05634896755309462	5.582874515505885E-4	0.0	1.6917801562139042E-5	3.6373273358598945E-4
21	0.05881050768038585	5.878936042843317E-4	0.0	1.6917801562139042E-5	3.6373273358598945E-4
22	0.05998206543856398	6.132703066275403E-4	0.0	1.6917801562139042E-5	3.8910943592919796E-4
23	0.06131857176197296	6.344175585802141E-4	0.0	2.1147251952673805E-5	4.0602723749133703E-4
24	0.06341214970528766	6.344175585802141E-4	0.0	2.5376702343208564E-5	4.271744894440108E-4
25	0.06525619007556083	6.55564810532888E-4	0.0	2.5376702343208564E-5	4.356333902250804E-4
26	0.06755701108801174	6.89400413657166E-4	0.0	2.5376702343208564E-5	4.440922910061499E-4
27	0.07009468132233258	7.232360167814441E-4	0.0	2.5376702343208564E-5	4.652395429588237E-4
28	0.07593978176205163	7.401538183435831E-4	0.0	2.5376702343208564E-5	4.8215734452096274E-4
29	0.07928527702096462	7.486127191246527E-4	0.0	3.3835603124278083E-5	5.075340468641713E-4
30	0.08291414545604345	7.909072230300003E-4	0.0	3.3835603124278083E-5	5.286812988168451E-4
31	0.08854777337623575	7.993661238110698E-4	0.0	3.3835603124278083E-5	5.329107492073799E-4
32	0.09268840530856928	8.332017269353478E-4	0.0	3.3835603124278083E-5	5.752052531127275E-4
33	0.096972838554181	8.67037330059626E-4	0.0	3.3835603124278083E-5	6.00581955455936E-4
34	0.1014391381665857	8.797256812312302E-4	0.0	3.3835603124278083E-5	6.00581955455936E-4
35	0.10609999249695501	9.051023835744388E-4	0.0	3.3835603124278083E-5	6.386470089707488E-4
36	0.11071432287302843	9.220201851365778E-4	0.0	3.806505351481284E-5	6.640237113139574E-4
37	0.11576428663932693	9.347085363081821E-4	0.0	4.229450390534761E-5	6.72482612095027E-4
38	0.12124988379585051	9.516263378703211E-4	0.0	4.229450390534761E-5	6.767120624855618E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	8475	0.0	14.103258	12
CAACGCA	8975	0.0	13.488926	13
GTATCAA	9285	0.0	13.062316	9
ATCAACG	9705	0.0	12.564887	11
AACGCAG	10125	0.0	12.130608	14
GGTATCA	10375	0.0	11.795873	8
CGAGATC	4605	0.0	11.737729	18
AGAGTAC	10600	0.0	11.319444	19
ACGCAGA	10960	0.0	11.2866125	15
AGTGGTA	11040	0.0	11.264507	5
TATCAAC	11040	0.0	11.24451	10
AATGCCG	5085	0.0	11.191004	13
GAGATCG	4975	0.0	10.910439	19
AGCGGTT	4885	0.0	10.860303	30
GTGGTAT	11505	0.0	10.771032	6
GCGGTTC	5305	0.0	10.374322	31
GATCGGA	5280	0.0	10.365781	21
TGGTATC	12045	0.0	10.306344	7
CGGTTCA	5550	0.0	10.305224	1
AAGCAGT	12990	0.0	10.131827	1
>>END_MODULE