FastQCFastQC Report
Fri 27 May 2016
SRR522038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27896730
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC109050.017.4480512
CAACGCA117000.016.3751513
GTATCAA121050.015.9725459
ATCAACG127850.015.05421411
AACGCAG129150.015.00502214
GGTATCA130400.014.8272748
AGTGGTA138000.014.1702425
AGAGTAC137350.014.03150419
TATCAAC139900.013.93032910
ACGCAGA140200.013.80671515
GAGTACT77500.013.38393120
GTGGTAT146950.013.2025396
CGAGATC59450.013.15730118
AAGCAGT154500.013.0639531
TGGTATC155100.012.6220957
GTACTTT82450.012.5846522
GAGATCG61450.012.51640119
CGCAGAG158150.012.26744216
CAGAGTA172200.011.407042518
GATCGGA66950.011.35909421
Produced by FastQC (version 0.11.5)