##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522038_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27896730
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.11872477526936	40.0	39.0	40.0	38.0	40.0
2	39.11260556344776	40.0	39.0	40.0	38.0	40.0
3	39.103758899340534	40.0	39.0	40.0	38.0	40.0
4	39.10128075942951	40.0	39.0	40.0	38.0	40.0
5	39.090137231137845	40.0	39.0	40.0	38.0	40.0
6	39.14055267409478	40.0	39.0	40.0	38.0	40.0
7	39.11765404045564	40.0	39.0	40.0	38.0	40.0
8	39.09993074457114	40.0	39.0	40.0	38.0	40.0
9	39.07683897001548	40.0	39.0	40.0	38.0	40.0
10	39.05121521411291	40.0	39.0	40.0	38.0	40.0
11	39.08462640603397	40.0	39.0	40.0	38.0	40.0
12	39.04572880047231	40.0	39.0	40.0	38.0	40.0
13	39.01279440278484	40.0	39.0	40.0	38.0	40.0
14	38.97853522617167	40.0	39.0	40.0	38.0	40.0
15	38.948105100490274	40.0	39.0	40.0	38.0	40.0
16	38.889179592016696	40.0	39.0	40.0	37.0	40.0
17	38.83201357291697	40.0	39.0	40.0	37.0	40.0
18	38.75388624401498	40.0	39.0	40.0	36.0	40.0
19	38.7243328877614	40.0	39.0	40.0	36.0	40.0
20	38.66724046151646	40.0	39.0	40.0	36.0	40.0
21	38.539345220748096	40.0	39.0	40.0	36.0	40.0
22	38.44994148059647	40.0	38.0	40.0	35.0	40.0
23	38.35861038193365	40.0	38.0	40.0	35.0	40.0
24	38.28002486312912	40.0	38.0	40.0	35.0	40.0
25	38.4897442101637	40.0	39.0	40.0	36.0	40.0
26	38.39596755605406	40.0	39.0	40.0	36.0	40.0
27	38.31525307804893	40.0	39.0	40.0	36.0	40.0
28	38.359852678073736	40.0	39.0	40.0	36.0	40.0
29	38.29126772205918	40.0	39.0	40.0	36.0	40.0
30	38.21329611033264	40.0	39.0	40.0	35.0	40.0
31	38.11518848266446	40.0	39.0	40.0	35.0	40.0
32	38.03547806499185	40.0	39.0	40.0	35.0	40.0
33	37.975133250384545	40.0	39.0	40.0	35.0	40.0
34	37.90734727690306	40.0	39.0	40.0	35.0	40.0
35	37.80884935259437	40.0	38.0	40.0	35.0	40.0
36	37.637175396542894	40.0	38.0	40.0	35.0	40.0
37	37.54724869187177	40.0	38.0	40.0	34.0	40.0
38	37.46552302008156	40.0	38.0	40.0	34.0	40.0
39	37.386834944454066	40.0	38.0	40.0	33.0	40.0
40	37.27219193790813	40.0	38.0	40.0	33.0	40.0
41	37.02409683859004	40.0	38.0	40.0	33.0	40.0
42	36.917172514484676	40.0	38.0	40.0	33.0	40.0
43	36.75853617968844	39.0	38.0	40.0	33.0	40.0
44	36.71246888076129	39.0	38.0	40.0	32.0	40.0
45	36.57839617761652	39.0	37.0	40.0	32.0	40.0
46	36.324410674656136	39.0	37.0	40.0	31.0	40.0
47	36.200656815332835	39.0	37.0	40.0	31.0	40.0
48	36.074694704361406	39.0	36.0	40.0	31.0	40.0
49	35.958863135571804	39.0	36.0	40.0	30.0	40.0
50	35.8410254535209	39.0	36.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.18340088355192563
1	2	-0.16243319584974358
1	3	-0.17808919355959318
1	4	-0.1870257146586667
1	5	-0.18060137790296693
1	6	-0.16196903946890018
1	7	-0.18560041786211912
1	8	-0.1913273832994662
1	9	-0.20610984189282533
1	10	-0.19637983392419045
1	11	-0.20723030949433507
1	12	-0.21047512103358912
1	13	-0.2070107532226615
1	14	-0.2047662621844779
1	15	-0.2201889494990681
1	16	-0.2077023717384776
1	17	-0.1988252694401922
1	18	-0.17648482940219168
1	19	-0.21266312465964887
1	20	-0.21069411047019315
1	21	-0.1739812483194143
1	22	-0.1841761287252126
1	23	-0.21736449062239416
1	24	-0.19273777202535314
1	25	-0.24039264449535125
1	26	-0.22503531344136007
1	27	-0.24272658965934824
1	28	-0.21871645472757706
1	29	-0.24266098690212345
1	30	-0.2017023018877424
1	31	-0.14422447668019345
1	32	-0.14855451872622183
1	33	-0.14545851448539082
1	34	-0.14695506387780455
1	35	-0.13698065502658352
1	36	-0.09736068749219129
1	37	-0.1171878165206266
1	38	-0.12930873263111664
1	39	-0.10213365716226974
1	40	-0.06786025516061756
1	41	-0.03913622503628744
1	42	0.01042368326937293
1	43	0.05316592062382597
1	44	-0.06265296480781046
1	45	-0.005002486517653892
1	46	0.020216590092523745
1	47	0.0032860677208788047
1	48	0.030500172568750372
1	49	-0.0077791204490793575
1	50	0.03383825712991495
2	1	-0.16759897368855547
2	2	-0.16526082175909096
2	3	-0.17056614025003114
2	4	-0.17042427593725762
2	5	-0.1634440487101827
2	6	-0.17628840723686068
2	7	-0.18887107204354692
2	8	-0.20104721579213702
2	9	-0.20718275939595543
2	10	-0.2058689576106758
2	11	-0.19063044622600955
2	12	-0.1744922072297186
2	13	-0.17304420206092885
2	14	-0.18624543946522465
2	15	-0.2052346130220144
2	16	-0.1971199175662548
2	17	-0.19126740676639287
2	18	-0.1792131610228438
2	19	-0.18159872784512032
2	20	-0.200757374758048
2	21	-0.20905249088503552
2	22	-0.19965213710431584
2	23	-0.22003647942309357
2	24	-0.25034929347672374
2	25	-0.3074745675463504
2	26	-0.2717641278840546
2	27	-0.3024303807121669
2	28	-0.25435074521090684
2	29	-0.2303949641792684
2	30	-0.22185318207878169
2	31	-0.17121029030239754
2	32	-0.18613862254043312
2	33	-0.19499483385688876
2	34	-0.1994908150336272
2	35	-0.18210634409631865
2	36	-0.16340956151010033
2	37	-0.20913708489413807
2	38	-0.2080749559356292
2	39	-0.15255956426588568
2	40	-0.20258501688607566
2	41	-0.09928658913062804
2	42	-0.08149296236012304
2	43	0.01018179518109008
2	44	-0.03643549693779846
2	45	-0.05004988507621988
2	46	0.00449298432181422
2	47	-0.016695824754783928
2	48	-0.02245522632052399
2	49	-0.02276859903692241
2	50	-0.05112457626812983
3	1	-0.17117897146086847
3	2	-0.16247824650633902
3	3	-0.1715701887759593
3	4	-0.19151147015234926
3	5	-0.17590289804769554
3	6	-0.17412494717335392
3	7	-0.16792323108693807
3	8	-0.1556966291455808
3	9	-0.17017025119760376
3	10	-0.18846348977901073
3	11	-0.1787815456821562
3	12	-0.17121650117680076
3	13	-0.18511246317095242
3	14	-0.17087289520381432
3	15	-0.21315311985445362
3	16	-0.1972544644439438
3	17	-0.18269670943765703
3	18	-0.17207125228438258
3	19	-0.18996136886154602
3	20	-0.20291350087593685
3	21	-0.15474868505507544
3	22	-0.17292472826869698
3	23	-0.16722116743812876
3	24	-0.15697409478857338
3	25	-0.21423165350317674
3	26	-0.20153154355197245
3	27	-0.23073130437197165
3	28	-0.18513971579690747
3	29	-0.21161224161190972
3	30	-0.17951212223726287
3	31	-0.10880262104330995
3	32	-0.14436456914732076
3	33	-0.09905658493279645
3	34	-0.15273062247990055
3	35	-0.1426474834269129
3	36	-0.11037663711068291
3	37	-0.08983457117632554
3	38	-0.0895633737740198
3	39	-0.1362480787293876
3	40	-0.09442123676569736
3	41	-0.052314465310075775
3	42	-0.014728455395569995
3	43	-0.025717300672070564
3	44	-0.006125546181777963
3	45	-0.04546857363352075
3	46	-0.006980489237705001
3	47	4.668890621388755E-4
3	48	0.04380990071966551
3	49	0.04226424963228226
3	50	0.03180870071898312
4	1	-0.19102122297475432
4	2	-0.1980395613382484
4	3	-0.17586453811828306
4	4	-0.1936519245743753
4	5	-0.19642430654029397
4	6	-0.18576449725006938
4	7	-0.171289086001579
4	8	-0.1817535431214239
4	9	-0.1821369937110191
4	10	-0.1916368911825117
4	11	-0.157349696722747
4	12	-0.1684160101090626
4	13	-0.17582936259540816
4	14	-0.17941509445086723
4	15	-0.201098533533262
4	16	-0.18083399345071882
4	17	-0.177128647332502
4	18	-0.15546689243200973
4	19	-0.16760995914732746
4	20	-0.15881009624042974
4	21	-0.16154808145252986
4	22	-0.1327151983231758
4	23	-0.13448925919628607
4	24	-0.14951820602964006
4	25	-0.17677477099766747
4	26	-0.183411098581594
4	27	-0.1929796457395554
4	28	-0.1530650576020065
4	29	-0.1425767519727259
4	30	-0.1221322891034049
4	31	-0.0847817987354702
4	32	-0.07970346569170061
4	33	-0.07875397355244473
4	34	-0.09648031737118856
4	35	-0.09955612066966069
4	36	-0.03814796205864468
4	37	-0.07354369947559292
4	38	-0.05178287117846736
4	39	-0.07982415701205525
4	40	-0.05043056029300175
4	41	-0.01655221930855788
4	42	0.014010978021154585
4	43	0.09090706319906161
4	44	0.05370653876465781
4	45	0.0018279167932249152
4	46	0.11859658646211102
4	47	0.13947198367185365
4	48	0.1202078536937563
4	49	0.04973215331831682
4	50	0.07378133527519282
5	1	-0.15658898189658288
5	2	-0.14768840968695685
5	3	-0.17220699549012863
5	4	-0.15397656405030347
5	5	-0.15498744014629295
5	6	-0.14338227828395844
5	7	-0.1455730959612751
5	8	-0.1480993780678972
5	9	-0.15968470031320692
5	10	-0.16677197423816637
5	11	-0.15343397011789506
5	12	-0.15167128339824387
5	13	-0.14914435881433263
5	14	-0.14622613403456342
5	15	-0.14531099711233253
5	16	-0.14682594535948112
5	17	-0.13138074044891823
5	18	-0.11314673873908276
5	19	-0.13466413709761582
5	20	-0.13870413220250555
5	21	-0.12875176058791027
5	22	-0.12459450789559412
5	23	-0.12746693241038543
5	24	-0.15201054090019284
5	25	-0.17488688512908368
5	26	-0.150608080516335
5	27	-0.14962668082652186
5	28	-0.11835291815651061
5	29	-0.08201085454726353
5	30	-0.12606120264268839
5	31	-0.09924220878237122
5	32	-0.10222877179188572
5	33	-0.0732111696535398
5	34	-0.09286926294991815
5	35	-0.05049315346558103
5	36	-0.025203935790884202
5	37	-0.033486527464525295
5	38	-0.03047603213571648
5	39	-0.02322142220924661
5	40	-0.03235330238391754
5	41	0.03490655383763652
5	42	0.05887033704210154
5	43	0.11975063741402181
5	44	0.028985905071870377
5	45	0.056452321546125006
5	46	0.09698576726562891
5	47	0.096551082697232
5	48	0.09172920373208626
5	49	0.09462176271212286
5	50	0.06489032183096555
6	1	-0.10135372032952006
6	2	-0.12314221880792786
6	3	-0.10947174435915485
6	4	-0.10648467714566578
6	5	-0.10626069090240264
6	6	-0.1078447879514286
6	7	-0.1268068735223693
6	8	-0.13277822761102698
6	9	-0.11819279930978865
6	10	-0.1441355846007042
6	11	-0.15322119035699444
6	12	-0.16185905869462403
6	13	-0.1755554096322669
6	14	-0.1620031877299084
6	15	-0.1560302697242406
6	16	-0.12123621754749081
6	17	-0.1339289542845563
6	18	-0.08829365499695996
6	19	-0.1282846182729216
6	20	-0.11908639541361765
6	21	-0.12369546585640734
6	22	-0.11097000517519007
6	23	-0.10366825005764468
6	24	-0.1276046190946758
6	25	-0.13953672832729325
6	26	-0.14834761377836259
6	27	-0.15691036880917864
6	28	-0.10529302217538117
6	29	-0.10540482942965212
6	30	-0.11606557930227979
6	31	-0.10420179001452823
6	32	-0.10085899929595854
6	33	-0.06409654620559735
6	34	-0.08694710065365285
6	35	-0.08482950899801978
6	36	-0.06888280242274902
6	37	-0.05563013393575034
6	38	-0.06664533560221741
6	39	-0.06368303838658562
6	40	-0.07019274218575333
6	41	-0.03418010911665448
6	42	-0.0355073429841255
6	43	0.07223176186565183
6	44	-0.001085523137312805
6	45	-0.046574729334643905
6	46	-0.010762380110236336
6	47	0.020733355685493393
6	48	-0.05412620566495008
6	49	-0.06382796520266965
6	50	-0.010247312920931506
7	1	-0.1059829017089271
7	2	-0.11275280852905922
7	3	-0.11931397899899565
7	4	-0.11728366450724792
7	5	-0.12029694534804491
7	6	-0.11684281576454936
7	7	-0.11495591705154595
7	8	-0.12451407378010515
7	9	-0.11288504550748257
7	10	-0.13038054449813785
7	11	-0.10915131846864057
7	12	-0.129236058860684
7	13	-0.12050179626866253
7	14	-0.1072371081817991
7	15	-0.12198877428684085
7	16	-0.160402186103056
7	17	-0.12385197209608378
7	18	-0.0951774393033773
7	19	-0.13958102614471812
7	20	-0.14890357710154944
7	21	-0.10105626614428331
7	22	-0.10839201788689934
7	23	-0.10979240152062175
7	24	-0.14982523840122752
7	25	-0.135171841553813
7	26	-0.16860118286351877
7	27	-0.1731117930778936
7	28	-0.14976183164839085
7	29	-0.13037004690232834
7	30	-0.09946555165876703
7	31	-0.10932203465149115
7	32	-0.09508035979278873
7	33	-0.16621306233930255
7	34	-0.12816129173880597
7	35	-0.12724128733214002
7	36	-0.09339103005906679
7	37	-0.08736083850418197
7	38	-0.09025594095856349
7	39	-0.07745708776442939
7	40	-0.0546941626938775
7	41	-0.01747937387241194
7	42	-0.05202771824773578
7	43	0.07391910023522996
7	44	0.005182927063003717
7	45	0.005984030715410427
7	46	0.010391563400389714
7	47	0.04721794201419982
7	48	0.02130491632867404
7	49	0.036158451346373965
7	50	0.0707647227605932
8	1	-0.11334474638177028
8	2	-0.11194173048239975
8	3	-0.10550835789953084
8	4	-0.10037593878377749
8	5	-0.12362702996549757
8	6	-0.11972401640446151
8	7	-0.11613059469611642
8	8	-0.14534970578444018
8	9	-0.10250876222141159
8	10	-0.11662775457295993
8	11	-0.09330001837246726
8	12	-0.10000569733134768
8	13	-0.10214460249643054
8	14	-0.13624236819374147
8	15	-0.10802872822331011
8	16	-0.09134184138077472
8	17	-0.1044499911783987
8	18	-0.08718277398129004
8	19	-0.12264741758666986
8	20	-0.08982135823296744
8	21	-0.06392099234533077
8	22	-0.06632831675749173
8	23	-0.0991805390056939
8	24	-0.06143616294187382
8	25	-0.09890131091312782
8	26	-0.09390380445977087
8	27	-0.1378131628756165
8	28	-0.08071343318798796
8	29	-0.08126980846587628
8	30	-0.0364187904268789
8	31	-0.04472589118065429
8	32	-0.025747382057751622
8	33	-0.04566945861903804
8	34	-0.018273014935637377
8	35	-0.020293866266051452
8	36	-5.308854568326637E-4
8	37	0.035987788833907075
8	38	-0.002127137479867258
8	39	0.037259310858686945
8	40	0.0026602963575541594
8	41	0.09524930366416129
8	42	0.07095211973494742
8	43	0.1687407181502607
8	44	0.07460633643219694
8	45	0.07019029750220085
8	46	0.11641820629041177
8	47	0.11050486253484593
8	48	0.14276463022794417
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114	4	-0.022869291184747453
114	5	-0.02279087545318248
114	6	-0.028376900131135585
114	7	-0.020739203381467064
114	8	-0.033166327941920315
114	9	-0.04234694056918897
114	10	-0.030260279649880317
114	11	-0.018844837159129213
114	12	-0.01670655313021996
114	13	-0.021743879390527354
114	14	-0.026190429491521172
114	15	-0.04249935670819838
114	16	9.9057229943611E-4
114	17	0.015891178373102832
114	18	0.05378710493277339
114	19	0.03550529179845796
114	20	0.008493952132404559
114	21	0.013395140055706634
114	22	0.018540908660668265
114	23	0.022405051644952323
114	24	0.07661241578597355
114	25	0.046640213284433685
114	26	0.046773078195876394
114	27	0.04936758746877956
114	28	0.08856953667573464
114	29	0.10372565773287334
114	30	0.11200595969603455
114	31	0.1403021813201093
114	32	0.2049555004544672
114	33	0.18005502002834817
114	34	0.20038582483716993
114	35	0.21530614897516642
114	36	0.2642399420272241
114	37	0.24101702169309647
114	38	0.2956140429615104
114	39	0.2856168223549247
114	40	0.2884758009793629
114	41	0.3880415496610681
114	42	0.36885189976184307
114	43	0.45354968666183737
114	44	0.4289811812428894
114	45	0.493305932399565
114	46	0.5447283733210142
114	47	0.5845527498824623
114	48	0.6209868724801595
114	49	0.6288155592486362
114	50	0.6648244079173864
115	1	-0.03685931463230929
115	2	-0.02343608476227388
115	3	-0.027155521396295512
115	4	-0.03286285833553393
115	5	-0.02868130605586572
115	6	-0.02733705706984324
115	7	-0.017064252310163397
115	8	-0.026075736400557048
115	9	-0.02613243575260782
115	10	-0.01661407673672244
115	11	-0.005973210364160764
115	12	-0.0165118394759034
115	13	-0.04066572334868823
115	14	-0.014087142832416077
115	15	0.009198922194237014
115	16	-0.032790946396602294
115	17	-0.0028949840324585807
115	18	0.02723482351961337
115	19	0.017981118837923304
115	20	-0.01125126520472719
115	21	0.010267752974804978
115	22	0.013579191999319562
115	23	0.05815070902431074
115	24	0.03034176252012344
115	25	-0.014330523907112536
115	26	0.013074109364488606
115	27	-0.006827142292827659
115	28	0.02929993631215666
115	29	0.09442109671862653
115	30	0.08456573592102501
115	31	0.14353901967818672
115	32	0.17228988702563441
115	33	0.1671652166032942
115	34	0.19201260638460838
115	35	0.1818752994196089
115	36	0.22025993564918167
115	37	0.24217931931310233
115	38	0.24772645261107584
115	39	0.2805476118996424
115	40	0.309134687600924
115	41	0.3945824799085571
115	42	0.42370659242522635
115	43	0.5039594382393204
115	44	0.4266052440138992
115	45	0.4777137115050749
115	46	0.4785988847979681
115	47	0.5060244786231607
115	48	0.5550598716162938
115	49	0.5263487500548152
115	50	0.5552592331773312
116	1	-0.05023261165732862
116	2	-0.04322900537911778
116	3	-0.035816970107646284
116	4	-0.04992802768793325
116	5	-0.04778537235365121
116	6	-0.04627960885549243
116	7	-0.08718003508473515
116	8	-0.04770414748916352
116	9	-0.0524929652107744
116	10	-0.051850771394370554
116	11	-0.061152221179582966
116	12	-0.034357870438242344
116	13	-0.053603332593411324
116	14	-0.05682209258121418
116	15	-0.0624383343592001
116	16	-0.03639846114917589
116	17	0.0025192678561438697
116	18	-0.011524160917367965
116	19	0.010345204229849969
116	20	-0.014380315899721552
116	21	0.049896468913978254
116	22	0.041199165546437655
116	23	0.07033822162100023
116	24	0.0867639798486195
116	25	0.03724662254916211
116	26	0.033496124546083195
116	27	0.08049025130218013
116	28	0.1481366547217533
116	29	0.14545166065634163
116	30	0.15877095479602588
116	31	0.17919889865980565
116	32	0.20668373645704463
116	33	0.22333974092329356
116	34	0.23338236940689683
116	35	0.24915512451148203
116	36	0.2749325795523916
116	37	0.32359936007129164
116	38	0.3232801992410046
116	39	0.3359124935910245
116	40	0.3687072053484144
116	41	0.42691166881518683
116	42	0.45764386269248547
116	43	0.5381284469202399
116	44	0.49733591847262915
116	45	0.5151934508690701
116	46	0.5507460516785514
116	47	0.5799590968658066
116	48	0.6384725388417607
116	49	0.6478202773701511
116	50	0.6829816950798886
117	1	-0.03149871578824559
117	2	-0.024819159027792637
117	3	-0.031746143849041175
117	4	-0.04112454813579092
117	5	-0.03542929852135046
117	6	-0.05434668646397256
117	7	-0.04372836380116496
117	8	-0.060595374564591964
117	9	-0.04138240320612141
117	10	-0.042103259229321566
117	11	-0.04902947924009737
117	12	-0.0532624417738603
117	13	-0.045050939488952224
117	14	-0.039980480089305104
117	15	-0.033625574874086794
117	16	-0.03762843241921843
117	17	-0.030899113789111254
117	18	0.01543567294525161
117	19	-0.006323129524027138
117	20	0.002573614375727118
117	21	0.02106895198154035
117	22	0.047640513794121375
117	23	0.014553851206855484
117	24	0.023740820737913282
117	25	-0.018964498876023583
117	26	-0.03300524705258567
117	27	-0.020983863344497422
117	28	0.041282272818236265
117	29	0.018399460835667014
117	30	0.07102931167005977
117	31	0.05724451874219483
117	32	0.08652741274109133
117	33	0.05813776522270331
117	34	0.10188624373481758
117	35	0.1408415100980207
117	36	0.1919837842907839
117	37	0.23067640379983345
117	38	0.250809130756501
117	39	0.2561461642740852
117	40	0.31402919034414367
117	41	0.35468026401234454
117	42	0.39769732646836786
117	43	0.4805230135885168
117	44	0.45381232269050287
117	45	0.4772119570273432
117	46	0.4613290269581469
117	47	0.4939678877121878
117	48	0.5122466146552895
117	49	0.5691755642298375
117	50	0.5477491449288223
118	1	-0.04176130079207496
118	2	-0.05424679084203632
118	3	-0.04485145478883368
118	4	-0.05514167796408742
118	5	-0.04106396072565843
118	6	-0.03496883276662999
118	7	-0.033271052725176276
118	8	-0.050368496837982946
118	9	-0.029629223677247296
118	10	-0.04741714118060969
118	11	-0.03832469208968092
118	12	-0.04000691173003901
118	13	-0.03663895100066128
118	14	-0.04327350828058485
118	15	-0.06339316752941926
118	16	-0.023230150650874748
118	17	-0.021808279320303825
118	18	0.004480373684117467
118	19	-0.016793500972205777
118	20	0.007446831756787731
118	21	0.009212714215692586
118	22	0.021967119890874187
118	23	0.03209457948329941
118	24	0.02986132547771092
118	25	-0.0020626170275903633
118	26	-0.039469409135541866
118	27	-0.03650799553324191
118	28	0.030287432870338193
118	29	0.07836675926447612
118	30	0.08234436167388282
118	31	0.1221712802490984
118	32	0.14908421091711688
118	33	0.15399387116018204
118	34	0.15217823760961835
118	35	0.16628685524928244
118	36	0.25218545780093393
118	37	0.24319023378766502
118	38	0.24955021528848675
118	39	0.29758685761594705
118	40	0.3275967566157405
118	41	0.33519883453764976
118	42	0.3943650524679754
118	43	0.5118783915422682
118	44	0.4520765419063082
118	45	0.44934475459083956
118	46	0.5206996148699972
118	47	0.5277007182875408
118	48	0.5859029930224153
118	49	0.5675120239584075
118	50	0.4841308864797611
119	1	-0.04789982784330249
119	2	-0.05427165041201931
119	3	-0.09574083484093876
119	4	-0.06077273059061383
119	5	-0.051367963869502375
119	6	-0.05426837916829186
119	7	-0.05396035471614624
119	8	-0.05117631087059493
119	9	-0.05379370758886637
119	10	-0.04703490632383733
119	11	-0.05164703680873117
119	12	-0.049368954376539875
119	13	-0.03150183467275269
119	14	-0.050419902699886165
119	15	-0.04243614835127829
119	16	-0.039897849636844285
119	17	-0.04628533029679005
119	18	-0.0032119627781383997
119	19	-0.01113416407611112
119	20	-0.033190897531348185
119	21	0.00522047406723658
119	22	0.027394325531226116
119	23	0.04704241575943513
119	24	0.0194012976623128
119	25	-0.011809140967180554
119	26	-0.010016946564839202
119	27	-0.00937711479479475
119	28	0.021783493187875536
119	29	0.05216842035565605
119	30	0.030271050180132875
119	31	0.08727395147818129
119	32	0.06637354286512931
119	33	0.11733649673596602
119	34	0.10583512096881265
119	35	0.12228790828340408
119	36	0.20723804608637408
119	37	0.21677753576091874
119	38	0.2510168971880731
119	39	0.2739586337555764
119	40	0.3115492481119162
119	41	0.40112168174493235
119	42	0.3879518174983261
119	43	0.513348337469175
119	44	0.438050995676619
119	45	0.4638821491013516
119	46	0.4724675844926338
119	47	0.48856128614266225
119	48	0.5585711945912379
119	49	0.5128311801184964
119	50	0.5880346994566708
120	1	-0.06940730872549494
120	2	-0.0759742190687902
120	3	-0.07381177913548242
120	4	-0.07346086511296335
120	5	-0.06706406545625754
120	6	-0.07783624030547287
120	7	-0.0959406174607409
120	8	-0.07888688235165375
120	9	-0.07320439645147303
120	10	-0.06285029461328406
120	11	-0.08288936446085415
120	12	-0.05524740472966272
120	13	-0.07815111926350937
120	14	-0.039158328258821484
120	15	-0.050730087630093124
120	16	-0.028200103365918494
120	17	-0.03398543034163737
120	18	0.007729455349803516
120	19	-0.025333906577984067
120	20	-0.021679048000876833
120	21	-0.03768492939575907
120	22	0.0016737626971945474
120	23	0.0036564122409004085
120	24	5.644787648506622E-4
120	25	-0.0219873861276767
120	26	-0.036228034596696546
120	27	-0.06032076179256052
120	28	-0.019468663467733904
120	29	0.012731351991796203
120	30	0.04929857275826066
120	31	0.09486223369545144
120	32	0.12872651384812173
120	33	0.16211602840514416
120	34	0.13389251157126125
120	35	0.19308413320796802
120	36	0.25809205554170234
120	37	0.25779126289633325
120	38	0.284125967918321
120	39	0.3040194202014135
120	40	0.31328228953957193
120	41	0.33604969454111
120	42	0.39522234246793886
120	43	0.4762152939848434
120	44	0.4612184448604921
120	45	0.4487895842585914
120	46	0.49459054165389205
120	47	0.5320418897747814
120	48	0.5974949862772547
120	49	0.5997343047453327
120	50	0.5572753914805801
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14330.0
3	611.0
4	989.0
5	1111.0
6	2143.0
7	3738.0
8	4648.0
9	5043.0
10	7089.0
11	11143.0
12	10295.0
13	13485.0
14	19304.0
15	24455.0
16	25537.0
17	28156.0
18	36645.0
19	48613.0
20	70494.0
21	49846.0
22	69695.0
23	96494.0
24	110834.0
25	83939.0
26	112095.0
27	140939.0
28	159507.0
29	149831.0
30	165964.0
31	201740.0
32	234269.0
33	296370.0
34	418959.0
35	624079.0
36	1005658.0
37	1909887.0
38	4135474.0
39	1.7288583E7
40	314738.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.229363231975917	26.884517681916094	23.46227909538857	28.423839990719422
2	25.632595648307166	28.00127470137181	24.41613407736319	21.949995572957835
3	24.240314187361744	25.73416310800585	25.148101587533734	24.87742111709867
4	28.123048830454323	24.370755999000597	22.844412230394028	24.66178294015105
5	27.05653673387526	26.806511014014905	24.1761453761785	21.96080687593134
6	27.222017737456284	25.195338712804165	21.604569892781473	25.978073656958077
7	25.43464842320452	24.805246541576842	25.018806347122887	24.74129868809575
8	25.644557623778848	24.446194948296807	24.833229557729524	25.07601787019482
9	25.051198676890905	24.281179753158767	24.800670989349747	25.86695058060058
10	25.17434846636798	25.887228024457933	23.291743494700704	25.64668001447339
11	25.676253094896783	25.30905952059614	23.865940560058473	25.148746824448597
12	25.55496647814995	24.77203242100418	24.57186200676567	25.101139094080203
13	25.28891379025427	25.058506857255313	24.47187896215793	25.18070039033249
14	24.981953081956203	25.470487042746587	24.585759692981938	24.961800182315276
15	24.692754312064533	25.088592820735624	24.786854946798424	25.431797920401422
16	24.973181444563576	24.980379420813836	24.597427727192397	25.449011407430188
17	25.02977230664669	25.078545048111373	24.751721796784068	25.139960848457864
18	24.767960259141482	24.698475412709662	24.673576437094958	25.859987891053898
19	25.05482183754153	24.975023954420465	24.58758786424072	25.382566343797286
20	25.06968819849958	24.907011305615683	24.79024714369975	25.233053352184985
21	24.91805132128757	24.855757277340913	24.7026604697153	25.523530931656214
22	25.236551548019996	24.803944327688168	24.540052596962475	25.419451527329358
23	25.047240303791877	24.9050157491577	24.94244307486935	25.105300872181076
24	25.096469817312624	24.781847335254977	24.6616987420191	25.4599841054133
25	25.301858188684157	24.675653675174512	24.494855872468875	25.527632263672455
26	25.23283028461351	24.88761954053141	24.786022435328288	25.093527739526795
27	25.15138339516998	24.56050916128491	24.849034367526105	25.439073076019003
28	25.30644844172503	24.655606172516364	24.636690863963352	25.401254521795245
29	25.22837047543669	24.802884157827272	24.836798798042768	25.13194656869327
30	25.230909238525733	24.566119668939113	24.762293905385253	25.440677187149895
31	25.36991105021405	24.640459994131632	24.566285222562897	25.423343733091418
32	25.25695672119077	24.793586403323257	24.68882722825167	25.2606296472343
33	25.21101503756024	24.576624416445185	24.650809593950868	25.56155095204371
34	25.340166681889542	24.646154270361748	24.51222885326927	25.501450194479435
35	25.218907753017177	24.78377072037937	24.76207724013548	25.235244286467974
36	25.217288872975246	24.59002116378934	24.660066734351528	25.53262322888389
37	25.382581046258018	24.685660136249645	24.41997508818848	25.51178372930386
38	25.263483962671202	24.90689012533663	24.595250404017662	25.234375507974505
39	25.323915344280252	24.611125575198937	24.55192099849334	25.51303808202747
40	25.457092904433274	24.65340470234413	24.363455820466378	25.52604657275622
41	25.344246700055002	24.873252084051902	24.545365228267713	25.23713598762538
42	25.37468413530304	24.588318743488845	24.47978403040568	25.55721309080244
43	25.499598242065026	24.701909848402572	24.29663241972813	25.501859489804275
44	25.35201374054462	24.92189555267852	24.452057389737973	25.274033317038878
45	25.344706529449134	24.659164224268416	24.45963972469862	25.536489521583828
46	25.481324903385822	24.646703422987027	24.318042062972005	25.553929610655146
47	25.417569391595947	24.780826910060224	24.455061973626226	25.346541724717607
48	25.33088879339268	24.510379027047065	24.366829018615324	25.791903160944933
49	25.406478970403967	24.60714971991144	24.231502087388836	25.754869222295753
50	25.390915455628825	24.738721323293568	24.44722559525775	25.42313762581986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3381.0
1	2619.0
2	1857.0
3	1799.5
4	1742.0
5	1555.5
6	1369.0
7	1379.5
8	1390.0
9	1621.5
10	1853.0
11	3148.5
12	4444.0
13	5243.5
14	6043.0
15	8967.0
16	11891.0
17	17456.5
18	23022.0
19	32065.5
20	41109.0
21	58210.0
22	75311.0
23	108118.0
24	140925.0
25	164343.5
26	187762.0
27	228343.0
28	268924.0
29	307552.0
30	346180.0
31	405881.5
32	465583.0
33	537623.5
34	609664.0
35	692079.0
36	774494.0
37	862551.5
38	950609.0
39	1050566.0
40	1150523.0
41	1239344.5
42	1328166.0
43	1414006.0
44	1499846.0
45	1608143.5
46	1716441.0
47	1820137.5
48	1923834.0
49	2012592.5
50	2101351.0
51	2161225.0
52	2221099.0
53	2217397.5
54	2213696.0
55	2154868.0
56	2096040.0
57	2004549.0
58	1913058.0
59	1793911.5
60	1674765.0
61	1490438.5
62	1306112.0
63	1128483.5
64	950855.0
65	801929.5
66	653004.0
67	548985.0
68	444966.0
69	366297.5
70	287629.0
71	235102.0
72	182575.0
73	153434.5
74	124294.0
75	102511.5
76	80729.0
77	66039.5
78	51350.0
79	41312.0
80	31274.0
81	22944.0
82	14614.0
83	10799.0
84	6984.0
85	5063.5
86	3143.0
87	2308.5
88	1474.0
89	1051.5
90	629.0
91	434.0
92	239.0
93	174.0
94	109.0
95	83.5
96	58.0
97	56.0
98	54.0
99	160.0
100	266.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5012773898589549
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0790967256735827E-4
7	0.0020181576837141843
8	0.0
9	4.660044385130444E-5
10	4.301579432428102E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0011757650448636812
21	7.169299054046836E-6
22	2.2224827067545192E-4
23	0.0
24	0.0015772457918903039
25	0.015216837242214409
26	0.015066281962079427
27	0.036151190480031174
28	0.05216381991724478
29	0.07478654308228957
30	0.08441491171187447
31	0.12727298145696644
32	0.1587175271080159
33	0.16061022205828424
34	0.15741629932970638
35	0.16146695329524285
36	0.18640177540521774
37	0.18678533290460925
38	0.21313250692823138
39	0.20671239962533244
40	0.22779730814328417
41	0.24894674035272232
42	0.26632153661020486
43	0.28759284690356185
44	0.28495096020214555
45	0.3068782613589478
46	0.32259336488541845
47	0.3199514781840022
48	0.3265221407670361
49	0.3276979058118998
50	0.33298526386425936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.789673E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.588958851124644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.03694781744424	38.20182035335479
2	11.130320303534996	11.03881991063668
3	4.054826981468411	6.032239449974009
4	2.1076668687137796	4.180680224981053
5	1.2734130888125346	3.157361463080415
6	0.8283136407497356	2.4645126628178327
7	0.5960266758895478	2.0689439613401572
8	0.44577951126112186	1.7684593472481782
9	0.34443357402344693	1.5372092096275052
>10	1.966839461396001	17.878632044908322
>50	0.14124574593907976	4.800109836032779
>100	0.07291624645220439	6.298061577630958
>500	0.0011384545005145672	0.3552248540310795
>1k	1.1714927997291596E-4	0.08955490367957436
>5k	0.0	0.0
>10k+	1.448053440383545E-5	0.12837020065656263
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2546273344581963E-4	6.452369148642153E-5	0.0	0.0	1.0753948581070255E-5
2	1.362166820268899E-4	1.003701867566557E-4	0.0	7.169299054046836E-6	1.4338598108093672E-5
3	1.5055528013498357E-4	1.4338598108093673E-4	0.0	7.169299054046836E-6	2.150789716214051E-5
4	1.5055528013498357E-4	1.756478268241475E-4	0.0	1.792324763511709E-5	3.584649527023418E-5
5	1.6489387824307724E-4	2.2224827067545192E-4	0.0	1.792324763511709E-5	5.018509337832785E-5
6	1.756478268241475E-4	2.545101164186627E-4	0.0	2.150789716214051E-5	5.018509337832785E-5
7	1.8281712587819434E-4	2.8677196216187346E-4	0.0	2.150789716214051E-5	5.735439243237469E-5
8	1.9715572398628799E-4	3.369570555402013E-4	0.0	2.150789716214051E-5	7.169299054046836E-5
9	2.6526406499973295E-4	3.7638820033745893E-4	0.0	2.5092546689163926E-5	8.244693912153862E-5
10	4.910969852022083E-4	4.1223469560769307E-4	0.0	3.2261845743210763E-5	9.320088770260888E-5
11	5.412820785805361E-4	4.229886441887633E-4	0.0	3.2261845743210763E-5	1.182934343917728E-4
12	5.842978729048171E-4	4.516658404049507E-4	0.0	3.2261845743210763E-5	1.2904738297284305E-4
13	6.34482966283145E-4	5.090202328373254E-4	0.0	3.2261845743210763E-5	1.4338598108093673E-4
14	7.097606063506368E-4	5.305281299994659E-4	0.0	3.584649527023418E-5	1.6130922871605382E-4
15	0.0033731552049290365	5.448667281075596E-4	0.0	3.584649527023418E-5	1.7923247635117092E-4
16	0.00365992716709091	5.771285738507703E-4	0.0	3.584649527023418E-5	1.8998642493224118E-4
17	0.004140270203712048	6.093904195939811E-4	0.0	3.94311447972576E-5	2.1507897162140508E-4
18	0.004735322025197935	6.810834101344495E-4	0.0	3.94311447972576E-5	2.2224827067545192E-4
19	0.005581299313575462	7.133452558776602E-4	0.0	3.94311447972576E-5	2.2583292020247534E-4
20	0.06423691952425965	7.635303492559881E-4	0.0	3.94311447972576E-5	2.4375616783759243E-4
21	0.06614753772216314	7.957921949991988E-4	0.0	3.94311447972576E-5	2.616794154727095E-4
22	0.06701143825817578	8.352233397964564E-4	0.0	4.301579432428102E-5	2.8318731263485004E-4
23	0.06795778573330996	8.603158864856203E-4	0.0	4.301579432428102E-5	3.0827985932401397E-4
24	0.0691729819229709	8.782391341207375E-4	0.0	4.301579432428102E-5	3.1903380790508423E-4
25	0.07025913072965899	9.069163303369248E-4	0.0	4.301579432428102E-5	3.2620310695913107E-4
26	0.07181845327391419	9.463474751341824E-4	0.0	4.660044385130444E-5	3.4412635459424816E-4
27	0.07332400607526401	9.78609320877393E-4	0.0	4.660044385130444E-5	3.477110041212716E-4
28	0.07822422197870503	0.0010072865170935804	0.0	5.376974290535127E-5	3.6204960222936526E-4
29	0.08036067309681098	0.0010431330123638147	0.0	5.735439243237469E-5	3.728035508104355E-4
30	0.08295954400390297	0.0010574716104719084	0.0	6.810834101344495E-5	3.8714214891852913E-4
31	0.10038094070523677	0.0010753948581070255	0.0	6.810834101344495E-5	3.978960974995994E-4
32	0.10307659714955839	0.0011076567038502362	0.0	7.527764006749178E-5	4.2657329371578674E-4
33	0.10629919707435244	0.0011614264467555875	0.0	7.527764006749178E-5	4.4808119087792726E-4
34	0.10896617632245786	0.001208026890606892	0.0	8.244693912153862E-5	4.516658404049507E-4
35	0.1118338959440766	0.0012223654887149856	0.0	8.244693912153862E-5	4.946816347292317E-4
36	0.11489518664015459	0.0012582119839852199	0.0	8.603158864856204E-5	5.341127795264893E-4
37	0.1181858949059621	0.0012653812830392666	0.0	8.603158864856204E-5	5.520360271616064E-4
38	0.12159131195663435	0.0013048124278365243	0.0	8.961623817558546E-5	5.592053262156532E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	10905	0.0	17.44805	12
CAACGCA	11700	0.0	16.37515	13
GTATCAA	12105	0.0	15.972545	9
ATCAACG	12785	0.0	15.054214	11
AACGCAG	12915	0.0	15.005022	14
GGTATCA	13040	0.0	14.827274	8
AGTGGTA	13800	0.0	14.170242	5
AGAGTAC	13735	0.0	14.031504	19
TATCAAC	13990	0.0	13.930329	10
ACGCAGA	14020	0.0	13.806715	15
GAGTACT	7750	0.0	13.383931	20
GTGGTAT	14695	0.0	13.202539	6
CGAGATC	5945	0.0	13.157301	18
AAGCAGT	15450	0.0	13.063953	1
TGGTATC	15510	0.0	12.622095	7
GTACTTT	8245	0.0	12.58465	22
GAGATCG	6145	0.0	12.516401	19
CGCAGAG	15815	0.0	12.267442	16
CAGAGTA	17220	0.0	11.4070425	18
GATCGGA	6695	0.0	11.359094	21
>>END_MODULE