FastQCFastQC Report
Fri 27 May 2016
SRR522037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29153470
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380430.13049218497832332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC117350.019.80820512
CAACGCA122250.018.83453813
GAGTACT104600.018.13204420
ATCAACG135650.017.37890611
GTATCAA140800.016.805669
AACGCAG139950.016.73507314
GGTATCA142450.016.503128
ACGCAGA146200.015.88440415
AGTGGTA150600.015.8581955
GTACTTT120300.015.84389622
AGAGTAC148200.015.40651719
GTGGTAT155150.015.1665546
TATCAAC160550.014.73831810
CGCAGAG157250.014.7123916
TGGTATC166850.014.1688247
AAGCAGT180200.013.7843261
AGTACTT144600.013.48306521
CAGAGTA189600.012.28330618
ACTTTTT158550.012.26238424
TACTTTT160700.012.08298223
Produced by FastQC (version 0.11.5)