##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522037_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29153470
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.07576600658515	40.0	39.0	40.0	38.0	40.0
2	39.06777186386389	40.0	39.0	40.0	38.0	40.0
3	39.05272353514007	40.0	39.0	40.0	38.0	40.0
4	39.050135095410596	40.0	39.0	40.0	38.0	40.0
5	39.0417793490792	40.0	39.0	40.0	38.0	40.0
6	39.09077825041067	40.0	39.0	40.0	38.0	40.0
7	39.06429224377064	40.0	39.0	40.0	38.0	40.0
8	39.04759951388291	40.0	39.0	40.0	38.0	40.0
9	39.02243142926039	40.0	39.0	40.0	38.0	40.0
10	38.99676748599738	40.0	39.0	40.0	38.0	40.0
11	39.03074436765161	40.0	39.0	40.0	38.0	40.0
12	38.99336432335499	40.0	39.0	40.0	38.0	40.0
13	38.96000620166313	40.0	39.0	40.0	38.0	40.0
14	38.92500902979988	40.0	39.0	40.0	38.0	40.0
15	38.89093922610242	40.0	39.0	40.0	37.0	40.0
16	38.83279029906217	40.0	39.0	40.0	37.0	40.0
17	38.77698990891993	40.0	39.0	40.0	37.0	40.0
18	38.721779843016975	40.0	39.0	40.0	36.0	40.0
19	38.6681785735969	40.0	39.0	40.0	36.0	40.0
20	38.610311191086346	40.0	39.0	40.0	36.0	40.0
21	38.48279426085471	40.0	38.0	40.0	36.0	40.0
22	38.40052336823027	40.0	38.0	40.0	35.0	40.0
23	38.313682933798276	40.0	38.0	40.0	35.0	40.0
24	38.23249465672525	40.0	38.0	40.0	35.0	40.0
25	38.14005235054352	40.0	38.0	40.0	35.0	40.0
26	38.31907597963467	40.0	38.0	40.0	36.0	40.0
27	38.242506226531525	40.0	38.0	40.0	35.0	40.0
28	38.170894785423485	40.0	39.0	40.0	35.0	40.0
29	38.23187548514808	40.0	38.0	40.0	35.0	40.0
30	38.17813484981376	40.0	38.0	40.0	35.0	40.0
31	38.15365121201696	40.0	39.0	40.0	35.0	40.0
32	38.077656793513775	40.0	39.0	40.0	35.0	40.0
33	38.01673937956614	40.0	39.0	40.0	35.0	40.0
34	37.951886585027445	40.0	38.0	40.0	35.0	40.0
35	37.86568068912551	40.0	38.0	40.0	35.0	40.0
36	37.64041673255362	40.0	38.0	40.0	35.0	40.0
37	37.61908753915057	40.0	38.0	40.0	34.0	40.0
38	37.53342236790338	40.0	38.0	40.0	34.0	40.0
39	37.45553891183451	40.0	38.0	40.0	33.0	40.0
40	37.36087841344444	40.0	38.0	40.0	33.0	40.0
41	37.10374411690958	40.0	38.0	40.0	33.0	40.0
42	36.989397385628536	39.0	38.0	40.0	33.0	40.0
43	36.78276167468229	39.0	38.0	40.0	32.0	40.0
44	36.79051759533256	39.0	37.0	40.0	32.0	40.0
45	36.66013260171088	39.0	37.0	40.0	32.0	40.0
46	36.350653970179195	39.0	37.0	40.0	31.0	40.0
47	36.21894096997716	39.0	36.0	40.0	31.0	40.0
48	36.09131722570246	39.0	36.0	40.0	31.0	40.0
49	35.87257787837949	39.0	36.0	40.0	30.0	40.0
50	35.84306091178855	39.0	36.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.17469980504162663
1	2	-0.17704418286076162
1	3	-0.18504783392328505
1	4	-0.1793558663989856
1	5	-0.17263403903971408
1	6	-0.18748547508130997
1	7	-0.183722005620524
1	8	-0.20990617421637126
1	9	-0.21296646478384673
1	10	-0.2022653665739753
1	11	-0.17589807594985984
1	12	-0.19846826584370802
1	13	-0.22813740570224184
1	14	-0.20382240134962615
1	15	-0.22070103002950958
1	16	-0.19225845738309033
1	17	-0.22381417835769213
1	18	-0.24185009027027604
1	19	-0.2195135481816095
1	20	-0.23045532367371635
1	21	-0.19463788255651338
1	22	-0.2125669581964047
1	23	-0.24219072579546008
1	24	-0.25279833985406697
1	25	-0.24610694845015502
1	26	-0.23778417847018574
1	27	-0.2747904972069861
1	28	-0.08975618857584067
1	29	-0.23135722907027656
1	30	-0.20994019210508696
1	31	-0.12371061770908653
1	32	-0.11271777428778762
1	33	-0.11968805454833387
1	34	-0.13641462624611478
1	35	-0.1413301779984053
1	36	-0.028985850870149932
1	37	-0.09876730942882261
1	38	-0.13074932997797362
1	39	-0.09716528349426312
1	40	-0.09902684274230467
1	41	-0.0799265311406856
1	42	-0.06682426445296841
1	43	0.059535828793578105
1	44	-0.07224009916788532
1	45	-0.0702663518576685
1	46	0.04047296896475672
1	47	-0.0038539121433629475
1	48	0.054041047238378326
1	49	0.16257009505071807
1	50	0.006805510369339629
2	1	-0.1680542416590427
2	2	-0.16833340885408177
2	3	-0.17368922500983075
2	4	-0.17008731567949553
2	5	-0.1557567997742879
2	6	-0.18901877139776957
2	7	-0.18853677188401008
2	8	-0.17146317615622308
2	9	-0.1884930952536692
2	10	-0.16536841874237496
2	11	-0.16504386463505227
2	12	-0.18168719750675422
2	13	-0.19654022492888856
2	14	-0.1770119517088844
2	15	-0.17980486203648383
2	16	-0.19151569086715625
2	17	-0.17918472473722602
2	18	-0.18380182281794788
2	19	-0.20022240455061535
2	20	-0.17021029237490382
2	21	-0.15070961899464663
2	22	-0.14888090283271538
2	23	-0.15758269013953452
2	24	-0.1608067097901369
2	25	-0.16667242089136636
2	26	-0.14152341995814055
2	27	-0.19514258279703256
2	28	-0.007134377567545869
2	29	-0.1329945290097001
2	30	-0.11006568700526742
2	31	-0.09819849333174346
2	32	-0.09886059806806458
2	33	-0.11422708395919301
2	34	-0.12860613130165888
2	35	-0.10898169508835309
2	36	-0.04667985526268126
2	37	-0.13307758024152605
2	38	-0.10141490546764231
2	39	-0.1349690111215125
2	40	-0.09853707588969485
2	41	-0.06091514581225965
2	42	-0.01720520067352993
2	43	0.10719450954478305
2	44	-0.0648447595696311
2	45	-0.038971870778162554
2	46	0.020775403358648248
2	47	-0.03969441153545006
2	48	-0.02414900979879775
2	49	0.08924110155254539
2	50	-0.05063487073745421
3	1	-0.19867435447822146
3	2	-0.19964325381290138
3	3	-0.20309132139848884
3	4	-0.1996469522667823
3	5	-0.20015143192592433
3	6	-0.19891694803818893
3	7	-0.19699461868624013
3	8	-0.19703516016774358
3	9	-0.20902146457500237
3	10	-0.2142417856806702
3	11	-0.19690001034025784
3	12	-0.2171982253019067
3	13	-0.2011660386657752
3	14	-0.19037512615133068
3	15	-0.18406940507556158
3	16	-0.19338964357783794
3	17	-0.18838647558958144
3	18	-0.21660791265001222
3	19	-0.19538202518217673
3	20	-0.2038353181419481
3	21	-0.2260671442879314
3	22	-0.23724945639006023
3	23	-0.22179487941960474
3	24	-0.24247625425878994
3	25	-0.209392181665649
3	26	-0.2473890829485157
3	27	-0.23988075179220658
3	28	-0.08569946842165876
3	29	-0.2206882920311628
3	30	-0.22109238926051944
3	31	-0.1413376581337289
3	32	-0.1255923013715048
3	33	-0.16303956283052656
3	34	-0.15151209754568384
3	35	-0.1129786434949338
3	36	-0.021088166690773846
3	37	-0.11184933139460895
3	38	-0.11631919961253345
3	39	-0.07539790354903175
3	40	-0.06067338760689012
3	41	-0.08055555321573848
3	42	-0.06114301914310971
3	43	0.07269248578839438
3	44	-0.018330760522346168
3	45	-0.024757732674807187
3	46	0.03343512449497865
3	47	-0.0314601723546275
3	48	0.03556787250658999
3	49	0.15240603433752398
3	50	0.0022032480709910374
4	1	-0.13244171683709993
4	2	-0.14578919062836349
4	3	-0.14046083375178142
4	4	-0.15424651189959349
4	5	-0.144013644998239
4	6	-0.1443534893897649
4	7	-0.14857307941932163
4	8	-0.16071421078747505
4	9	-0.14727978430771316
4	10	-0.14643023803000688
4	11	-0.18789393633851859
4	12	-0.16967459078620806
4	13	-0.14636253929142384
4	14	-0.17670714349225847
4	15	-0.16166383145468188
4	16	-0.15907493030741193
4	17	-0.1671705136038355
4	18	-0.16478758739545896
4	19	-0.1811822504304601
4	20	-0.18848639713942816
4	21	-0.1378636369193842
4	22	-0.17973982188822646
4	23	-0.14365629091153664
4	24	-0.1508832400185085
4	25	-0.17482362147343622
4	26	-0.18546922103335106
4	27	-0.20061337999661788
4	28	-0.05860768073154077
4	29	-0.13887193782134233
4	30	-0.14198267855325497
4	31	-0.11204861494453411
4	32	-0.093949194394078
4	33	-0.12701140444398362
4	34	-0.10288237106354359
4	35	-0.07537095361033153
4	36	-0.025624950264003132
4	37	-0.08964229603019902
4	38	-0.056583913051362345
4	39	-0.06936697388290725
4	40	-0.05187555419974643
4	41	-0.03777935764944118
4	42	-0.0481479484112981
4	43	0.10439835293868072
4	44	-0.023689445503478623
4	45	0.0060412124215147855
4	46	0.024626735950519674
4	47	0.0623405444923435
4	48	0.11037623957219722
4	49	0.21357472633868468
4	50	0.12546633096033588
5	1	-0.12621262055718319
5	2	-0.13643539797313053
5	3	-0.13999921888402156
5	4	-0.1277371676378607
5	5	-0.12981492665186778
5	6	-0.11851092717103029
5	7	-0.1205918486067219
5	8	-0.12864007660654408
5	9	-0.11622497912123464
5	10	-0.12070308951599884
5	11	-0.12547185387940374
5	12	-0.15341662156848912
5	13	-0.1414146131031373
5	14	-0.14803451170972437
5	15	-0.14127095346087515
5	16	-0.1225900955709065
5	17	-0.13371705838770254
5	18	-0.12657972418545427
5	19	-0.14370867252335273
5	20	-0.13721934468723163
5	21	-0.09388949598658058
5	22	-0.10827749238597306
5	23	-0.0992401537009755
5	24	-0.10749290904296771
5	25	-0.15932088098013963
5	26	-0.14250334133890874
5	27	-0.1791821472418107
5	28	0.020947080658814343
5	29	-0.10402934647564877
5	30	-0.10835351046618058
5	31	-0.03643524542458465
5	32	-0.02709640487666576
5	33	-0.048621615882574076
5	34	-0.04185118000690835
5	35	-0.030829104320694967
5	36	0.08019991504060897
5	37	0.018904712751428576
5	38	-0.016013607148366305
5	39	-0.008479447145944619
5	40	0.004207282804713941
5	41	-0.01773126190861518
5	42	-0.03689610260232001
5	43	0.13208077109069194
5	44	0.03251428366764486
5	45	0.03784917660532727
5	46	0.14345153134965472
5	47	0.0934626428730212
5	48	0.11733034862297842
5	49	0.24073834406858907
5	50	0.1569181397021211
6	1	-0.14094047940913157
6	2	-0.15786517357840069
6	3	-0.14833290614112116
6	4	-0.1511399770189712
6	5	-0.1994525019443003
6	6	-0.1642787050031984
6	7	-0.1557813186379775
6	8	-0.14948493002928842
6	9	-0.15397742020557814
6	10	-0.17118794520389002
6	11	-0.17511307798506692
6	12	-0.1749214388142093
6	13	-0.189424595020661
6	14	-0.16256687555905813
6	15	-0.2261832268188968
6	16	-0.1724254014999218
6	17	-0.17453181077991786
6	18	-0.1744672188557459
6	19	-0.16593411749986586
6	20	-0.16894312010956014
6	21	-0.15001858312969318
6	22	-0.14752795683722297
6	23	-0.19163791917984696
6	24	-0.15664712893378407
6	25	-0.14906567983047836
6	26	-0.1630391626471166
6	27	-0.15044811780378353
6	28	-0.014922289953638312
6	29	-0.12742552328337808
6	30	-0.10915518259609769
6	31	-0.09339130008584817
6	32	-0.08796655358354144
6	33	-0.0765626747834176
6	34	-0.09221304485605941
6	35	-0.05585959171131094
6	36	0.055184030502346104
6	37	-0.05170761789969447
6	38	-0.03173720694088189
6	39	0.020322189776806
6	40	-0.00606974714979458
6	41	0.023913276552647744
6	42	0.026432058017789473
6	43	0.14001588193824688
6	44	-0.009901634658355363
6	45	0.029609562062958616
6	46	0.07437348265373345
6	47	0.07805506431640907
6	48	0.1553468004811549
6	49	0.24136154441050195
6	50	0.07278569015010561
7	1	-0.13159490295667808
7	2	-0.11248173045207466
7	3	-0.1095942526785052
7	4	-0.12928436774276975
7	5	-0.11696047695585321
7	6	-0.10880469895137423
7	7	-0.10316630690256545
7	8	-0.1087715299883314
7	9	-0.12219375535534738
7	10	-0.09998172390382365
7	11	-0.12026151474068314
7	12	-0.11250700789994283
7	13	-0.09785883537014683
7	14	-0.12529256804022992
7	15	-0.12400801027506247
7	16	-0.10954312193138094
7	17	-0.13741775438634107
7	18	-0.129278018563042
7	19	-0.11573954692936894
7	20	-0.1424169571767422
7	21	-0.12269815769063541
7	22	-0.13804521390570557
7	23	-0.14585740255139967
7	24	-0.17844922929165108
7	25	-0.10141878767105794
7	26	-0.12002155974863626
7	27	-0.10959254007275376
7	28	0.002249830756440474
7	29	-0.07499282880192482
7	30	-0.08544547512462941
7	31	-0.04184360410534538
7	32	-0.051983656665562705
7	33	-0.04187905047621854
7	34	-0.018686609236183926
7	35	-0.0024472081038027227
7	36	0.0887471727046858
7	37	-4.767057933747765E-4
7	38	0.0036330018370662742
7	39	0.033351291133875804
7	40	0.020408084766060597
7	41	0.053609016007136745
7	42	0.06298764657687173
7	43	0.15638404322567823
7	44	0.06795186091056138
7	45	0.07347131251130179
7	46	0.14460821021840076
7	47	0.14617390975554656
7	48	0.15712693671957112
7	49	0.29076455495864195
7	50	0.16233677759956322
8	1	-0.0968354730360943
8	2	-0.11392753772717867
8	3	-0.09810189493759935
8	4	-0.1053716952561814
8	5	-0.10402080899823574
8	6	-0.10394822768608236
8	7	-0.11662413951459172
8	8	-0.12368590005608837
8	9	-0.1244823586924042
8	10	-0.11660364389991429
8	11	-0.1194720034637271
8	12	-0.1275219941379433
8	13	-0.1280449413263156
8	14	-0.1289097717578116
8	15	-0.11621695863551196
8	16	-0.11670681019359819
8	17	-0.13038423572425728
8	18	-0.09169478687061172
8	19	-0.14421648460877634
8	20	-0.150413142437678
8	21	-0.1098922687807331
8	22	-0.13514081838251712
8	23	-0.1224242984358952
8	24	-0.11780939693769454
8	25	-0.12639786952284027
8	26	-0.13172482410464426
8	27	-0.16591336926354927
8	28	-0.02620734848056827
8	29	-0.10386170261196526
8	30	-0.12247267546265306
8	31	-0.02523136634295753
8	32	-0.061096047341983706
8	33	-0.049664817279612805
8	34	-0.0570546682807489
8	35	-0.06381751650675938
8	36	0.018914180822974913
8	37	-0.054397097138675576
8	38	-0.01267954167511931
8	39	-0.006007213062233063
8	40	-0.003463595750943682
8	41	0.04750982958140071
8	42	0.03931604300569802
8	43	0.15417733362475872
8	44	0.056519896749072984
8	45	0.060882149638239014
8	46	0.09446013200118131
8	47	0.1327469743332088
8	48	0.15504058979265523
8	49	0.2391117138292671
8	50	0.13604241225233693
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116	19	-0.01968334099889546
116	20	0.004625771443805604
116	21	0.027032378898461218
116	22	0.0400813237040083
116	23	0.03951009468908495
116	24	0.061838027322217215
116	25	0.075602973975613
116	26	0.006852303132831139
116	27	0.006333338665100996
116	28	0.1675560026310734
116	29	0.1050356366217926
116	30	0.10478433549141641
116	31	0.16778657912868056
116	32	0.14650940128179712
116	33	0.1803978601473375
116	34	0.18496059476387927
116	35	0.1942311970876247
116	36	0.3145049266924005
116	37	0.22516018733842458
116	38	0.2180947450722286
116	39	0.28822443718023294
116	40	0.2808804706245027
116	41	0.39788546396376745
116	42	0.41454244080458835
116	43	0.5159232583261328
116	44	0.4274772214142004
116	45	0.47195888796594687
116	46	0.5294866641992684
116	47	0.5385254917194615
116	48	0.5734723375586981
116	49	0.6999921431932421
116	50	0.6650655275741997
117	1	-0.005657450447067447
117	2	-0.0026043685392878047
117	3	0.005533014203251696
117	4	-8.197918257053516E-4
117	5	0.0013520952610832637
117	6	-0.0041934217938788265
117	7	0.003593025151261031
117	8	-9.007157333087434E-4
117	9	-0.006870053065824777
117	10	0.0031937101749193175
117	11	-0.017856035167731932
117	12	-0.02478463331410552
117	13	-0.0073833528812770055
117	14	-0.0030752884014688675
117	15	-0.0019816445844043074
117	16	0.02294772496165365
117	17	0.014792213809769805
117	18	0.04548426286466167
117	19	0.025993465008035344
117	20	0.03688938469692715
117	21	0.052687657362405105
117	22	0.042113972949870515
117	23	0.03629308407322185
117	24	0.05746000531829765
117	25	0.08737672321064593
117	26	1.9058419842110652E-4
117	27	-0.03303078495418532
117	28	0.21765607698527134
117	29	0.10075926638391763
117	30	0.1233927474076637
117	31	0.15767810059457332
117	32	0.16822909146848986
117	33	0.1859486282963445
117	34	0.19276715939717803
117	35	0.23420271700679507
117	36	0.38012839054147207
117	37	0.3106078421870109
117	38	0.2900909898620512
117	39	0.3077953588463487
117	40	0.3661669879278051
117	41	0.45030630394589366
117	42	0.4320665287462546
117	43	0.5733054535591862
117	44	0.536271916614858
117	45	0.5001070789237971
117	46	0.6669813120110675
117	47	0.6684652587133257
117	48	0.6932867871023802
117	49	0.7945668793767098
117	50	0.7485176678231085
118	1	-0.03808408783972084
118	2	-0.04860203869461088
118	3	-0.048374430815997016
118	4	-0.04783598573808945
118	5	-0.05970606504043019
118	6	-0.051984425340137363
118	7	-0.051544035357473206
118	8	-0.05113454123674899
118	9	-0.047523823711472346
118	10	-0.05652524459987518
118	11	-0.04717798156114128
118	12	-0.04306763686101789
118	13	-0.04451337814929701
118	14	-0.04858490802575233
118	15	-0.030987373896877557
118	16	-0.03392108177732922
118	17	-0.03245286265488545
118	18	-0.05185661962413235
118	19	-0.024164872473413368
118	20	-0.016204783169612824
118	21	3.0985328381660793E-4
118	22	-0.021097822208794526
118	23	0.0010803328244293198
118	24	-0.03788891404497008
118	25	0.021833421681222376
118	26	-0.045153589225201074
118	27	-0.006688966647075745
118	28	0.14458664045072567
118	29	0.03250655777439704
118	30	0.023228873223082758
118	31	0.07015848300235916
118	32	0.07658680260003337
118	33	0.11293299085319575
118	34	0.07092645927311736
118	35	0.14475698976823992
118	36	0.22324990578127313
118	37	0.17704669967452702
118	38	0.19711683244772615
118	39	0.17032800365566203
118	40	0.2425343728957685
118	41	0.3368529047253901
118	42	0.38310943845372947
118	43	0.5000024442394277
118	44	0.4278385313193951
118	45	0.4523548486039033
118	46	0.6287951894918606
118	47	0.6144058485163626
118	48	0.6087021724468613
118	49	0.7809737555081995
118	50	0.7011655801341163
119	1	-0.07285620339219179
119	2	-0.0798369338324747
119	3	-0.08131506835501057
119	4	-0.0722434299381689
119	5	-0.07374315301206025
119	6	-0.08741644450054054
119	7	-0.08678247583434029
119	8	-0.07051528489203207
119	9	-0.07962234338572216
119	10	-0.09867287574530081
119	11	-0.0735014526596558
119	12	-0.08451499512175076
119	13	-0.08143667742875493
119	14	-0.07306950411517477
119	15	-0.06548421896374634
119	16	-0.07143162742211473
119	17	-0.08852540998101688
119	18	-0.05962720348193784
119	19	-0.04554234430278825
119	20	-0.05556528334071231
119	21	-0.06781656174440798
119	22	-0.045662262182737834
119	23	-0.009306862353049894
119	24	-0.02043947056638018
119	25	0.01125137770973339
119	26	-0.030862388625173764
119	27	-0.011204387811979188
119	28	0.167789218439502
119	29	0.05479753193795034
119	30	0.0806922687455014
119	31	0.13568757002351362
119	32	0.1432978326015899
119	33	0.14602430290342028
119	34	0.15613072658872085
119	35	0.1907012820301972
119	36	0.2998185721077533
119	37	0.24194405983642753
119	38	0.21414645515195474
119	39	0.265819793097009
119	40	0.2712519143836474
119	41	0.3633199435743677
119	42	0.3989365780680032
119	43	0.4852392551405842
119	44	0.4155739797141962
119	45	0.4284700215833652
119	46	0.5422619738927068
119	47	0.5600240365836768
119	48	0.5839566125880182
119	49	0.6961637070544171
119	50	0.602559242810031
120	1	-0.06757040978490636
120	2	-0.09143013834273006
120	3	-0.07337220199299566
120	4	-0.07644996916130964
120	5	-0.0687186194068019
120	6	-0.07831039420202757
120	7	-0.09273967349976431
120	8	-0.08960908524787214
120	9	-0.08549763728186832
120	10	-0.07923343278454809
120	11	-0.07213890836785453
120	12	-0.06008591509643679
120	13	-0.07733184157749662
120	14	-0.0636210550535381
120	15	-0.06073683060652968
120	16	-0.09663999189781691
120	17	-0.07442462256339866
120	18	-0.0695618391895394
120	19	-0.07179904958331207
120	20	-0.05239812487734241
120	21	-0.06987607379412708
120	22	-0.038892843293382384
120	23	-0.039522222200766066
120	24	2.1244952299781517E-4
120	25	0.026266971230043623
120	26	-0.05345298312685287
120	27	-0.028971512533701116
120	28	0.15261514784373986
120	29	0.01380876510320661
120	30	0.07225750341755344
120	31	0.1039340156045796
120	32	0.14344512838996337
120	33	0.18880515641535567
120	34	0.1715215274971129
120	35	0.194740476037353
120	36	0.27539847542723805
120	37	0.18433898005369542
120	38	0.22019954937588437
120	39	0.23921490036202187
120	40	0.28662899433312816
120	41	0.37520699795292245
120	42	0.41153875976415577
120	43	0.5399240682880091
120	44	0.4550390832498721
120	45	0.40241571080225924
120	46	0.5283010315238954
120	47	0.5721237554699599
120	48	0.5927541380643362
120	49	0.6982470236734173
120	50	0.6463657056400436
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16360.0
3	696.0
4	1141.0
5	1323.0
6	2328.0
7	4117.0
8	5112.0
9	5657.0
10	7585.0
11	12213.0
12	11407.0
13	14482.0
14	20588.0
15	25095.0
16	25458.0
17	28040.0
18	36274.0
19	46599.0
20	60335.0
21	45823.0
22	60804.0
23	81024.0
24	89936.0
25	85008.0
26	114227.0
27	144744.0
28	163751.0
29	160490.0
30	182685.0
31	222308.0
32	262173.0
33	334494.0
34	475497.0
35	713453.0
36	1139118.0
37	2164976.0
38	4623972.0
39	1.748166E7
40	282517.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.06435874946764	28.859074932478375	22.25646863516643	29.820097682887553
2	24.85418373867673	28.257847179083655	25.26711228543292	21.62085679680669
3	23.376459131623097	26.557843714659008	25.371614425315407	24.694082728402485
4	25.283559727195424	25.694389038423214	24.564990033776425	24.457061200604937
5	25.604063598604217	27.0824090579955	25.43794958198801	21.875577761412277
6	25.501174441888846	25.902983190103722	23.521657251762758	25.074185116244674
7	24.764052399207507	25.731040950614357	25.694149617731355	23.810757032446777
8	24.130837643920536	25.373227300240032	25.93424564272301	24.56168941311642
9	23.872797416345442	25.07461814188401	26.191491753303975	24.86109268846657
10	24.210629075072646	26.29366427349227	24.67453957793289	24.8211670735022
11	24.33137100492559	26.114973926595752	24.998952097009955	24.554702971468707
12	24.277593713544217	25.69150773475679	25.509995894142275	24.520902657556718
13	24.294665437767783	25.876768700261067	25.284681377551284	24.543884484419863
14	24.22928042528042	26.125706476793333	25.373631337881907	24.271381760044346
15	23.977893540631698	25.960696959915918	25.430513074429907	24.630896425022474
16	24.13790193757381	25.81948906939723	25.37456776157349	24.66804123145547
17	24.377767037680247	25.764977548127206	25.516729226400837	24.34052618779171
18	24.084038709628732	25.62208546701302	25.433270893653482	24.860604929704767
19	24.27937394759526	25.813568676387405	25.35882349511053	24.5482338809068
20	24.412798516976178	25.67757684873148	25.523814384794584	24.38581024949775
21	24.208418414686143	25.698021539116954	25.432608879834888	24.660951166362015
22	24.426009411144374	25.57392027093734	25.345256691101515	24.65481362681677
23	24.19842646518579	25.698999124289497	25.71373493446921	24.38883947605551
24	24.256743346728978	25.650076660396127	25.45370750037029	24.63947249250461
25	24.36503479277858	25.538280290198838	25.304681681155223	24.79200323586735
26	24.337984705269168	25.676904918021986	25.64502602870829	24.340084348000552
27	24.335638257334846	25.43751973674887	25.6066239780426	24.620218027873687
28	24.3983935286453	25.56459060694211	25.41011243899161	24.62690342542098
29	24.355275599547074	25.65946539155835	25.5892791868191	24.395979822075482
30	24.36272650441947	25.44987424546593	25.51648693166353	24.670912318451073
31	24.454571256407643	25.533769193390288	25.35603704855137	24.655622501650694
32	24.433516072060655	25.662145454649142	25.475106547966142	24.429231925324057
33	24.394423941224773	25.470128597694874	25.441758553781263	24.693688907299087
34	24.49584317295303	25.566331267562898	25.295779517759687	24.642046041724385
35	24.47249379923896	25.63352419963214	25.462158899732728	24.43182310139617
36	24.432190650557338	25.501957579314844	25.389578439728716	24.676273330399106
37	24.527233571906628	25.544649786518526	25.2812218163862	24.64689482518865
38	24.497546379573983	25.636190431208618	25.43086985421964	24.43539333499776
39	24.455208208359274	25.420722415095277	25.418917632110972	24.705151744434477
40	24.565898869872083	25.481801490723942	25.28205752001995	24.670242119384024
41	24.51187135749947	25.58296256887474	25.418717876952517	24.48644819667327
42	24.472318882989956	25.394845077014704	25.406398280151453	24.726437759843886
43	24.587509001334432	25.486987114000435	25.234032975199504	24.691470909465625
44	24.54374082195766	25.581486154301952	25.381462196700166	24.493310827040222
45	24.517822397292733	25.37871417998524	25.382763743477042	24.72069967924498
46	24.58355066864719	25.448252149202222	25.23425242628109	24.7339447558695
47	24.54532406249676	25.55171524665645	25.354697868087722	24.548262822759067
48	24.535966735895183	25.348306516962367	25.31697730017041	24.79874944697204
49	24.62010040845769	25.46983407785116	25.209335729383504	24.700729784307644
50	24.60881814615435	25.50858339126542	25.329090861906263	24.553507600673967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5776.0
1	3993.5
2	2211.0
3	2136.0
4	2061.0
5	1989.0
6	1917.0
7	2093.5
8	2270.0
9	2684.0
10	3098.0
11	4717.0
12	6336.0
13	8778.5
14	11221.0
15	16740.0
16	22259.0
17	32502.0
18	42745.0
19	58865.5
20	74986.0
21	99657.0
22	124328.0
23	176280.5
24	228233.0
25	253648.0
26	279063.0
27	329553.5
28	380044.0
29	433964.0
30	487884.0
31	558924.5
32	629965.0
33	711934.5
34	793904.0
35	886568.5
36	979233.0
37	1074274.0
38	1169315.0
39	1274291.0
40	1379267.0
41	1470932.0
42	1562597.0
43	1646362.5
44	1730128.0
45	1822527.5
46	1914927.0
47	1992338.5
48	2069750.0
49	2135409.0
50	2201068.0
51	2219225.5
52	2237383.0
53	2199733.5
54	2162084.0
55	2076951.0
56	1991818.0
57	1879387.0
58	1766956.0
59	1627660.0
60	1488364.0
61	1314289.0
62	1140214.0
63	974963.0
64	809712.0
65	674865.5
66	540019.0
67	446956.0
68	353893.0
69	286403.0
70	218913.0
71	174373.0
72	129833.0
73	106706.0
74	83579.0
75	66414.0
76	49249.0
77	39830.5
78	30412.0
79	24950.0
80	19488.0
81	15176.0
82	10864.0
83	8982.0
84	7100.0
85	5771.5
86	4443.0
87	3370.0
88	2297.0
89	1720.5
90	1144.0
91	815.0
92	486.0
93	355.0
94	224.0
95	159.5
96	95.0
97	83.0
98	71.0
99	157.0
100	243.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4998650246437216
2	0.0
3	0.0
4	0.0
5	0.0
6	1.6464592379569225E-4
7	9.638646788872816E-4
8	1.920869110949743E-4
9	1.2691456625917944E-4
10	6.860246824820509E-6
11	2.7440987299282038E-5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	2.0923752815702558E-4
21	0.0
22	5.52249869398051E-4
23	0.0
24	7.409066570806151E-4
25	0.01776803927628512
26	0.01939391777376758
27	0.03601286570689527
28	0.05938915676247116
29	0.08168495894313782
30	0.09222229806606212
31	0.13719464612617296
32	0.15832420634662014
33	0.16748263585775552
34	0.16002897768258806
35	0.1680005844930295
36	0.1905193446955028
37	0.1932257120678945
38	0.21396080809591447
39	0.21987091073549736
40	0.23234283946302103
41	0.25275550389027446
42	0.2719470443827099
43	0.28871005749915873
44	0.2931109058372811
45	0.3039398054502603
46	0.3195228561128401
47	0.32104926103136266
48	0.33452278579531014
49	0.341444774841554
50	0.3407347392951851
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.915347E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.258845430271485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07890966797673	41.05133735940632
2	11.538967849704868	12.291042102646072
3	4.3075892668905285	6.882516928272572
4	2.080439503537626	4.432072237837646
5	1.1972810248971095	3.1882902520796086
6	0.7856447747437036	2.510552017270522
7	0.5688027947445874	2.120564608792589
8	0.41047166225010817	1.7488997450628085
9	0.2934340565124194	1.4065163153795242
>10	1.5612644553023265	14.900845908670519
>50	0.12061915688239273	4.383003084474191
>100	0.05635158761604877	4.85104413451713
>500	1.8538696215866465E-4	0.05797891743581683
>1k	2.5922480464875754E-5	0.02610253847836552
>5k	6.444749455120587E-6	0.01865508938389654
>10k+	6.444749425678907E-6	0.13057876029244886
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38043	0.13049218497832332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.08711107116923E-5	7.203259166061536E-5	0.0	3.430123412410255E-6	3.430123412410255E-6
2	4.116148094892306E-5	1.2691456625917944E-4	0.0	1.0290370237230766E-5	3.430123412410255E-6
3	4.802172777374357E-5	1.9551703450738453E-4	0.0	1.372049364964102E-5	6.86024682482051E-6
4	7.546271507302562E-5	2.4353876228112812E-4	0.0	2.4010863886871786E-5	6.86024682482051E-6
5	1.9551703450738453E-4	3.121412305293332E-4	0.0	2.4010863886871786E-5	1.7150617062051277E-5
6	1.9551703450738453E-4	3.7045332854030756E-4	0.0	2.4010863886871786E-5	1.7150617062051277E-5
7	1.989471579197948E-4	4.047545626644101E-4	0.0	2.4010863886871786E-5	2.058074047446153E-5
8	2.1266765156943582E-4	4.1504493290164086E-4	0.0	2.4010863886871786E-5	2.744098729928204E-5
9	2.538291325183589E-4	4.596365372629742E-4	0.0	2.744098729928204E-5	3.4301234124102554E-5
10	5.042281416243075E-4	5.11088388449128E-4	3.430123412410255E-6	2.744098729928204E-5	3.4301234124102554E-5
11	6.551535717703587E-4	5.556799928104613E-4	3.430123412410255E-6	2.744098729928204E-5	4.459160436133332E-5
12	8.197994955660509E-4	5.831209801097434E-4	3.430123412410255E-6	3.4301234124102554E-5	6.17422214233846E-5
13	9.913056661865636E-4	6.242824610586664E-4	3.430123412410255E-6	3.4301234124102554E-5	7.203259166061536E-5
14	0.0012485649221173328	6.448632015331279E-4	3.430123412410255E-6	3.4301234124102554E-5	8.918320872266664E-5
15	0.0017253520764423584	6.551535717703587E-4	3.430123412410255E-6	3.4301234124102554E-5	8.918320872266664E-5
16	0.0020923752815702554	6.86024682482051E-4	3.430123412410255E-6	3.773135753651281E-5	1.0290370237230765E-4
17	0.0024971298442346655	7.477669039054356E-4	3.430123412410255E-6	3.773135753651281E-5	1.0633382578471791E-4
18	0.003042519466807896	7.957886316791792E-4	3.430123412410255E-6	3.773135753651281E-5	1.0633382578471791E-4
19	0.003567328348906665	8.403802360405125E-4	3.430123412410255E-6	4.116148094892306E-5	1.1662419602194867E-4
20	0.008074510512813741	8.986923340514869E-4	3.430123412410255E-6	4.116148094892306E-5	1.234844428467692E-4
21	0.008544437420313945	9.81015295949333E-4	3.430123412410255E-6	4.459160436133332E-5	1.2691456625917944E-4
22	0.009034945068288612	9.913056661865636E-4	3.430123412410255E-6	4.459160436133332E-5	1.4406518332123072E-4
23	0.009498011728963996	0.0010290370237230765	3.430123412410255E-6	4.459160436133332E-5	1.680760472081025E-4
24	0.009995379623763484	0.0010461876407851277	3.430123412410255E-6	4.802172777374357E-5	1.74936294032923E-4
25	0.010468736654676099	0.0010736286280844099	3.430123412410255E-6	5.488197459856408E-5	1.7836641744533327E-4
26	0.01101069615383692	0.0011216503558581534	3.430123412410255E-6	5.488197459856408E-5	1.7836641744533327E-4
27	0.01156637614664738	0.0011559515899822559	3.430123412410255E-6	5.8312098010974337E-5	1.8522666427015378E-4
28	0.013912580560735995	0.0011868227006939483	3.430123412410255E-6	5.8312098010974337E-5	1.8865678768256402E-4
29	0.0145162822813202	0.001238274551880102	3.430123412410255E-6	5.8312098010974337E-5	1.8865678768256402E-4
30	0.015236608197926353	0.0012725757860042046	3.430123412410255E-6	5.8312098010974337E-5	1.989471579197948E-4
31	0.017630834339788713	0.0013034468967158968	3.430123412410255E-6	5.8312098010974337E-5	2.0237728133220504E-4
32	0.01832371926909558	0.0013617589947268712	3.430123412410255E-6	6.517234483579485E-5	2.0923752815702555E-4
33	0.019020034321814865	0.001409780722500615	3.430123412410255E-6	7.203259166061536E-5	2.2638814521907684E-4
34	0.019675187893585223	0.0014372217097998968	3.430123412410255E-6	7.889283848543587E-5	2.3667851545630761E-4
35	0.020361212576067274	0.001464662697099179	3.430123412410255E-6	8.232296189784613E-5	2.675496261679999E-4
36	0.021067817999023786	0.0014749530673364097	3.430123412410255E-6	8.232296189784613E-5	2.7440987299282043E-4
37	0.0217744234219803	0.001505824178048102	3.430123412410255E-6	8.232296189784613E-5	2.7440987299282043E-4
38	0.022498179461998863	0.0015126844248729225	3.430123412410255E-6	8.918320872266664E-5	2.7783999640523067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	11735	0.0	19.808205	12
CAACGCA	12225	0.0	18.834538	13
GAGTACT	10460	0.0	18.132044	20
ATCAACG	13565	0.0	17.378906	11
GTATCAA	14080	0.0	16.80566	9
AACGCAG	13995	0.0	16.735073	14
GGTATCA	14245	0.0	16.50312	8
ACGCAGA	14620	0.0	15.884404	15
AGTGGTA	15060	0.0	15.858195	5
GTACTTT	12030	0.0	15.843896	22
AGAGTAC	14820	0.0	15.406517	19
GTGGTAT	15515	0.0	15.166554	6
TATCAAC	16055	0.0	14.738318	10
CGCAGAG	15725	0.0	14.71239	16
TGGTATC	16685	0.0	14.168824	7
AAGCAGT	18020	0.0	13.784326	1
AGTACTT	14460	0.0	13.483065	21
CAGAGTA	18960	0.0	12.283306	18
ACTTTTT	15855	0.0	12.262384	24
TACTTTT	16070	0.0	12.082982	23
>>END_MODULE