FastQCFastQC Report
Fri 27 May 2016
SRR522036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20428401
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC56750.011.65249312
CAACGCA57900.011.15544713
GTATCAA68550.09.9992759
AACGCAG69400.09.59182714
ATCAACG70200.09.48251911
GGTATCA71450.09.3474418
GAGTACT50650.09.28498520
AGAGTAC78100.08.72187119
ACGCAGA76350.08.66115215
AGTGGTA83050.08.5180015
GTGGTAT82150.08.2101716
GTACTTT57400.08.19682122
TATCAAC85300.08.06147610
CGCAGAG84400.07.835154516
TGGTATC89100.07.74235967
AAGCAGT105950.07.56707571
TTCGCGA3857.408846E-46.8671736
CAGAGTA105600.06.511970518
ACTTTTT76100.06.503000724
TACTTTT79150.06.3352823
Produced by FastQC (version 0.11.5)