FastQCFastQC Report
Fri 27 May 2016
SRR522035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26428318
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC79300.09.7805912
CAACGCA83000.09.397531513
ATCAACG91600.08.70715611
GTATCAA94300.08.5044529
AACGCAG95650.08.36141814
GGTATCA100300.08.0395238
ACGCAGA102150.07.872532415
AGAGTAC106700.07.55836119
AGTGGTA108000.07.46637735
TATCAAC112350.07.39237310
GTGGTAT110550.07.25439076
CGCAGAG116200.06.92071216
AAGCAGT132600.06.8119651
TGGTATC121600.06.703567
GAGTACT61800.06.365426520
CAGAGTA141500.05.885164718
GAGTACA111950.05.632920717
GTACTTT73400.05.510600622
GTACATG114600.05.426599519
CAGTGGT162550.05.33922964
Produced by FastQC (version 0.11.5)