##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522035_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26428318
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.14927344222209	40.0	39.0	40.0	38.0	40.0
2	39.14938722169152	40.0	39.0	40.0	38.0	40.0
3	39.137607054675215	40.0	39.0	40.0	38.0	40.0
4	39.130359677070636	40.0	39.0	40.0	38.0	40.0
5	39.124621097717984	40.0	39.0	40.0	38.0	40.0
6	39.18217935776314	40.0	39.0	40.0	38.0	40.0
7	39.16216090634296	40.0	39.0	40.0	38.0	40.0
8	39.141285495353884	40.0	39.0	40.0	38.0	40.0
9	39.11836050254882	40.0	39.0	40.0	38.0	40.0
10	39.09778855392916	40.0	39.0	40.0	38.0	40.0
11	39.1308672765327	40.0	39.0	40.0	38.0	40.0
12	39.09611186758083	40.0	39.0	40.0	38.0	40.0
13	39.065564142220474	40.0	39.0	40.0	38.0	40.0
14	39.03708976106614	40.0	39.0	40.0	38.0	40.0
15	39.004303225048226	40.0	39.0	40.0	38.0	40.0
16	38.95589166892876	40.0	39.0	40.0	38.0	40.0
17	38.902111931603066	40.0	39.0	40.0	37.0	40.0
18	38.852398817056766	40.0	39.0	40.0	37.0	40.0
19	38.801567091783895	40.0	39.0	40.0	37.0	40.0
20	38.75278786186847	40.0	39.0	40.0	36.0	40.0
21	38.65802038555764	40.0	39.0	40.0	36.0	40.0
22	38.57722390808223	40.0	39.0	40.0	36.0	40.0
23	38.725468643142555	40.0	39.0	40.0	36.0	40.0
24	38.68037254584268	40.0	39.0	40.0	36.0	40.0
25	38.63424433594298	40.0	39.0	40.0	36.0	40.0
26	38.58019821768453	40.0	39.0	40.0	36.0	40.0
27	38.511344308782725	40.0	39.0	40.0	36.0	40.0
28	38.44349602574027	40.0	39.0	40.0	36.0	40.0
29	38.51974681097753	40.0	39.0	40.0	36.0	40.0
30	38.464269878998735	40.0	39.0	40.0	36.0	40.0
31	38.40891584549573	40.0	39.0	40.0	36.0	40.0
32	38.338086669004056	40.0	39.0	40.0	36.0	40.0
33	38.28067488063372	40.0	39.0	40.0	36.0	40.0
34	38.23396191918078	40.0	39.0	40.0	35.0	40.0
35	38.17603916374852	40.0	39.0	40.0	35.0	40.0
36	38.04130092577212	40.0	39.0	40.0	35.0	40.0
37	37.95324594626113	40.0	39.0	40.0	35.0	40.0
38	37.88564047095241	40.0	38.0	40.0	35.0	40.0
39	37.82266586167156	40.0	38.0	40.0	35.0	40.0
40	37.74069798917964	40.0	38.0	40.0	35.0	40.0
41	37.551892557067006	40.0	38.0	40.0	34.0	40.0
42	37.45079331193154	40.0	38.0	40.0	34.0	40.0
43	37.38065388799998	40.0	38.0	40.0	33.0	40.0
44	37.29067411705883	40.0	38.0	40.0	33.0	40.0
45	37.17072978310614	40.0	38.0	40.0	33.0	40.0
46	36.97794267497462	40.0	38.0	40.0	33.0	40.0
47	36.857988389575155	39.0	38.0	40.0	33.0	40.0
48	36.761644119765776	39.0	37.0	40.0	33.0	40.0
49	36.66913550835888	39.0	37.0	40.0	32.0	40.0
50	36.56287910566234	39.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1512301020283644
1	2	-0.15695857666764113
1	3	-0.14946932487818998
1	4	-0.14509463567965497
1	5	-0.1613016882678835
1	6	-0.15389030636919188
1	7	-0.14367183458971056
1	8	-0.1745146936387485
1	9	-0.16223997777055388
1	10	-0.1814066464665629
1	11	-0.1980234573412929
1	12	-0.18393207780129472
1	13	-0.19497712342057127
1	14	-0.1840698594771979
1	15	-0.19052517777988953
1	16	-0.15175927588574467
1	17	-0.1838921775540001
1	18	-0.16659569127745755
1	19	-0.20300012045868243
1	20	-0.20850488030539793
1	21	-0.1703658002911297
1	22	-0.18171597467338074
1	23	-0.24756669561563882
1	24	-0.2691185666967044
1	25	-0.21223486160339178
1	26	-0.3216679477685318
1	27	-0.34890479322126566
1	28	-0.2538785803419259
1	29	-0.2260681502844264
1	30	-0.25901320257489857
1	31	-0.12911783096196672
1	32	-0.1675759632203082
1	33	-0.16550969373155056
1	34	-0.16340301155765502
1	35	-0.12256188179808447
1	36	-0.09923185564309023
1	37	-0.10473205807931407
1	38	-0.16037806044833758
1	39	-0.17749334344140522
1	40	-0.13885207892901974
1	41	-0.11638946571740405
1	42	-0.15849010946489273
1	43	-0.09055672793550684
1	44	-0.11074666043015213
1	45	-0.10782067799707562
1	46	-0.09669441037252824
1	47	-0.07568670827470925
1	48	-0.14921696228371673
1	49	-0.13696740661227125
1	50	-0.09214839347801984
2	1	-0.15522367069593912
2	2	-0.14109154938351764
2	3	-0.14400918817369757
2	4	-0.14537382800053678
2	5	-0.14184030415263038
2	6	-0.15033034570311088
2	7	-0.1510872082777155
2	8	-0.13597390000887088
2	9	-0.1410930429001951
2	10	-0.1700635468932461
2	11	-0.14103469724584272
2	12	-0.1404827928486938
2	13	-0.16868955084700588
2	14	-0.15469786251821205
2	15	-0.16428919292835076
2	16	-0.1542434819707097
2	17	-0.14354420184302796
2	18	-0.16448145278427262
2	19	-0.16356114383641085
2	20	-0.16236970904094505
2	21	-0.1643841156649799
2	22	-0.14040291278615769
2	23	-0.18133238861307177
2	24	-0.23916204538088692
2	25	-0.21013337403157095
2	26	-0.22871971496923038
2	27	-0.27352359418204486
2	28	-0.23694344968204462
2	29	-0.1548440566785132
2	30	-0.18427408159705294
2	31	-0.17678659743261704
2	32	-0.17263238382864898
2	33	-0.18280815386921034
2	34	-0.19793146251751637
2	35	-0.1974711858125957
2	36	-0.18606207206133263
2	37	-0.21993975995165016
2	38	-0.24542121305086795
2	39	-0.21180485907386526
2	40	-0.21257037575987425
2	41	-0.15923617664660128
2	42	-0.21571707001823626
2	43	-0.18945892646320317
2	44	-0.2014425398752877
2	45	-0.18045087390943593
2	46	-0.20988814858951343
2	47	-0.17313068820452315
2	48	-0.18855599034558423
2	49	-0.201129961335198
2	50	-0.21164268397564
3	1	-0.1499251895552689
3	2	-0.14479730640411503
3	3	-0.14998350120910686
3	4	-0.14030750937357084
3	5	-0.12764776981425285
3	6	-0.13684080841362345
3	7	-0.1284793302419942
3	8	-0.14978889779166593
3	9	-0.14409407991295353
3	10	-0.12772363773558482
3	11	-0.16091599520455446
3	12	-0.16617648045734512
3	13	-0.17067417613441904
3	14	-0.16465782154773478
3	15	-0.17036360143852392
3	16	-0.15621716870307978
3	17	-0.16844863521803433
3	18	-0.15467603373969752
3	19	-0.18803056234759197
3	20	-0.14966440606720255
3	21	-0.17056398713879872
3	22	-0.1923517392564591
3	23	-0.22184776367323877
3	24	-0.20129364174913889
3	25	-0.25107057115450715
3	26	-0.2757608988920879
3	27	-0.2747651921751455
3	28	-0.3025411250797134
3	29	-0.17762531801118797
3	30	-0.22965389116748725
3	31	-0.2227174112656769
3	32	-0.23383481573706177
3	33	-0.22955909120357632
3	34	-0.2138154410627351
3	35	-0.1793141477258331
3	36	-0.18423537309320892
3	37	-0.1782174446896434
3	38	-0.2011791869893642
3	39	-0.22245258161307646
3	40	-0.2270973279920412
3	41	-0.1959999649707953
3	42	-0.21577515905833167
3	43	-0.23138627880715745
3	44	-0.19077668902721
3	45	-0.20485368292639095
3	46	-0.23153400975706973
3	47	-0.2632677431848265
3	48	-0.1999319137944866
3	49	-0.2285766330740131
3	50	-0.22605038957246393
4	1	-0.17152649610434878
4	2	-0.1752774631900067
4	3	-0.17135426717349844
4	4	-0.15276591211732438
4	5	-0.1607933520451894
4	6	-0.15715673198049984
4	7	-0.14971264724346867
4	8	-0.16302090824405724
4	9	-0.15843933550661404
4	10	-0.1485640754295261
4	11	-0.1438469767495505
4	12	-0.15094878161690417
4	13	-0.15446375640552645
4	14	-0.17021045914368216
4	15	-0.1908792963593129
4	16	-0.15838865326946916
4	17	-0.15272144266536003
4	18	-0.17252656452526338
4	19	-0.16410065870558554
4	20	-0.15889471800579713
4	21	-0.17112972288924766
4	22	-0.16247429355990306
4	23	-0.2117891649471204
4	24	-0.2009940500457219
4	25	-0.1462078567987959
4	26	-0.19793310206046755
4	27	-0.23665204502505333
4	28	-0.20928840582445218
4	29	-0.1367092964531338
4	30	-0.141471366261392
4	31	-0.11815252477212823
4	32	-0.10218235779161233
4	33	-0.1258974874145835
4	34	-0.14513492497584934
4	35	-0.14131277584930046
4	36	-0.11991311276330663
4	37	-0.10953797384202346
4	38	-0.16369854024756592
4	39	-0.18148331818012053
4	40	-0.19447168696640205
4	41	-0.1891744872637915
4	42	-0.2020252325517191
4	43	-0.19133199169299786
4	44	-0.24260631502753682
4	45	-0.21947043941830913
4	46	-0.1637209116025744
4	47	-0.15400189311133516
4	48	-0.19236836651374034
4	49	-0.18639476144246458
4	50	-0.16988386989256554
5	1	-0.11687416019621111
5	2	-0.11643882403165406
5	3	-0.11521159677437964
5	4	-0.1199278665080925
5	5	-0.1189243662579571
5	6	-0.11474045238115593
5	7	-0.1308034905513722
5	8	-0.12960147088710983
5	9	-0.12771370793220882
5	10	-0.13376361236800705
5	11	-0.1096589318287684
5	12	-0.12317291779851303
5	13	-0.12824010870510705
5	14	-0.13973729796502
5	15	-0.11848730482405756
5	16	-0.1225298213665269
5	17	-0.11682054460985825
5	18	-0.12406411985274701
5	19	-0.14949701331411802
5	20	-0.1110109299252855
5	21	-0.09479133210217583
5	22	-0.1254558878176013
5	23	-0.16863859432864814
5	24	-0.1836892468945308
5	25	-0.17895212418822837
5	26	-0.21028095235136846
5	27	-0.2309903252365899
5	28	-0.28448154421015204
5	29	-0.14121135767003068
5	30	-0.15619386213334252
5	31	-0.10822878333669905
5	32	-0.12527622307375452
5	33	-0.1440573368454494
5	34	-0.11579200165108716
5	35	-0.10172867203539937
5	36	-0.08007866085889503
5	37	-0.058062026456489946
5	38	-0.12697065244148575
5	39	-0.10954370004700564
5	40	-0.050951510215007545
5	41	-0.009389631663360376
5	42	-0.07069430095285156
5	43	-0.053250761889714227
5	44	-0.0768617322224543
5	45	-0.078445305938601
5	46	-0.05627347491077472
5	47	-0.07786820008977458
5	48	-0.07441339871727592
5	49	-0.08303641643197324
5	50	-0.06647927804232268
6	1	-0.125003111860309
6	2	-0.13746699699051135
6	3	-0.15171518747172996
6	4	-0.13708912669457618
6	5	-0.1540199673209628
6	6	-0.15661068580339332
6	7	-0.13411577210007408
6	8	-0.127986007893945
6	9	-0.15335574313799327
6	10	-0.1541007271889896
6	11	-0.16368951658656528
6	12	-0.14053933235100402
6	13	-0.15319432961247514
6	14	-0.1451301303529391
6	15	-0.13804703790238193
6	16	-0.14505315161601828
6	17	-0.1573490851187671
6	18	-0.160665732261684
6	19	-0.21607683023336222
6	20	-0.15586483381671457
6	21	-0.1699232661612129
6	22	-0.19913035466555584
6	23	-0.21035396922741967
6	24	-0.22739526481718997
6	25	-0.18730635508772053
6	26	-0.23927708833346628
6	27	-0.2536725572053129
6	28	-0.25458190353181465
6	29	-0.20153023781565338
6	30	-0.192922310899867
6	31	-0.1936214801262892
6	32	-0.19814458292342607
6	33	-0.1785057566960404
6	34	-0.1751845739576936
6	35	-0.19241646658197453
6	36	-0.1543900582490494
6	37	-0.19497221601307757
6	38	-0.17733459326470324
6	39	-0.192924606111049
6	40	-0.20814240066597733
6	41	-0.1607609280068658
6	42	-0.15241421509524855
6	43	-0.19224210223250537
6	44	-0.18323762313707448
6	45	-0.16939269703223658
6	46	-0.1676069117455654
6	47	-0.15050819329775322
6	48	-0.18696028077118854
6	49	-0.18855882516461264
6	50	-0.23351598450621225
7	1	-0.09036293254078487
7	2	-0.0728247540706306
7	3	-0.08441893697150249
7	4	-0.0725718821899548
7	5	-0.08183791455641654
7	6	-0.06341001603378515
7	7	-0.07277478117227076
7	8	-0.0760626114857601
7	9	-0.08185500758895614
7	10	-0.10633423019967836
7	11	-0.06936519240110073
7	12	-0.07364684891496864
7	13	-0.0731330616081678
7	14	-0.09222153175475967
7	15	-0.06530453698455574
7	16	-0.04352643207391793
7	17	-0.05083823124881803
7	18	-0.08217976644512959
7	19	-0.08906394506887239
7	20	-0.05547949765723814
7	21	-0.05581125057077685
7	22	-0.054938480824091585
7	23	-0.08858985281447929
7	24	-0.08040989666157117
7	25	-0.08367033979461524
7	26	-0.08753096145832728
7	27	-0.08697230374531273
7	28	-0.14466955107226198
7	29	-0.04099464847181622
7	30	-0.007179027769055324
7	31	-0.010129517149124467
7	32	-0.006480399422983396
7	33	-0.027174790358550638
7	34	-0.012689032929962707
7	35	-0.016241083644274568
7	36	-0.0077094064929283945
7	37	0.016193506356124487
7	38	0.016080392531364396
7	39	0.0039015898047836117
7	40	0.011779369217990165
7	41	0.006162470206497517
7	42	0.043331775738622014
7	43	0.006100779539771395
7	44	0.027941273586719717
7	45	-0.005959010040214707
7	46	-0.033334299349291996
7	47	0.0369684721257002
7	48	-0.008663538608914223
7	49	0.020143771962736423
7	50	0.08033305985769346
8	1	-0.10094775482210139
8	2	-0.09017291476808964
8	3	-0.08529906043586521
8	4	-0.08152977294349739
8	5	-0.09620290133968723
8	6	-0.08855909377297877
8	7	-0.10507069989579776
8	8	-0.10584835260878833
8	9	-0.10757278906255152
8	10	-0.10413771177553599
8	11	-0.07862686984191924
8	12	-0.112279601909961
8	13	-0.08030397926980015
8	14	-0.09347060664260454
8	15	-0.07778585495490375
8	16	-0.0649739046704525
8	17	-0.07979030953498523
8	18	-0.08312908144368691
8	19	-0.08365555583769435
8	20	-0.12173466552151524
8	21	-0.07185827187996097
8	22	-0.08904386172806511
8	23	-0.07820435177693241
8	24	-0.10835856627900853
8	25	-0.08568305539232313
8	26	-0.12978083043494593
8	27	-0.11762818019145982
8	28	-0.0944299155876891
8	29	-0.07995151139304824
8	30	-0.05265704296600404
8	31	-0.055110406785921384
8	32	-0.031249391589675213
8	33	-0.02634425013005881
8	34	-0.04864817079229766
8	35	-0.023202302909083983
8	36	-0.05824222020741132
8	37	-0.05965028761009705
8	38	-0.0753333996323633
8	39	-0.0709269253753817
8	40	-0.03356640298896707
8	41	0.022430649990347717
8	42	-0.039435177417374234
8	43	0.005159612761701737
8	44	8.384943937755907E-4
8	45	-0.035084703248756455
8	46	-0.03517022484709997
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112	49	0.4879954511063289
112	50	0.4717076778102225
113	1	-0.020281558976620317
113	2	-0.035481225344462075
113	3	-0.050896154868866006
113	4	-0.0412762058606404
113	5	-0.04147940305572462
113	6	-0.029227338982146023
113	7	-0.037397839593580784
113	8	-0.026006309319932086
113	9	-0.03519266578542357
113	10	-0.03354196407518373
113	11	-0.026276176294764753
113	12	-0.024884858930285247
113	13	-0.023464625247690662
113	14	-0.024508302310884744
113	15	-0.038145925692916194
113	16	-0.018267566877369745
113	17	0.01757137072024051
113	18	0.033079671793018406
113	19	0.02764815087883221
113	20	0.025997026296671777
113	21	0.032634077616449986
113	22	0.07498975979243028
113	23	0.04932331956814551
113	24	0.008131614737322934
113	25	0.03729871551703923
113	26	0.033941810121987714
113	27	0.06142133310795117
113	28	0.06708201960275773
113	29	0.11278335741847911
113	30	0.12487687823622196
113	31	0.15028867735246365
113	32	0.15673797006054713
113	33	0.17644414404021802
113	34	0.15070467182707858
113	35	0.18385861344968646
113	36	0.24411067688185994
113	37	0.2384041904573877
113	38	0.24678421018091257
113	39	0.26166593091625145
113	40	0.2546625016334403
113	41	0.32344230243228367
113	42	0.37487835651308643
113	43	0.3589961964656325
113	44	0.38722512450138424
113	45	0.4586030591986727
113	46	0.47160641789563584
113	47	0.4523434903161814
113	48	0.5177976926421906
113	49	0.5396040965052364
113	50	0.5350677277927858
114	1	-0.049566150567528666
114	2	-0.06143130934385255
114	3	-0.041321194363959535
114	4	-0.0473847521008679
114	5	-0.04915665932614388
114	6	-0.05687284332014286
114	7	-0.05719576804423809
114	8	-0.06541311995391652
114	9	-0.04348307607976665
114	10	-0.05021003731078366
114	11	-0.05789573583360408
114	12	-0.04874217253970414
114	13	-0.047986641363706894
114	14	-0.03645195098517462
114	15	-0.03275210590270916
114	16	-0.023953441422492006
114	17	-0.028698626266077554
114	18	-0.021134709321643186
114	19	-0.004260373500592607
114	20	-0.017765525629542367
114	21	-0.0053236074360683006
114	22	0.03259308611923473
114	23	-0.018348989436354657
114	24	-0.006864117233440936
114	25	-0.02490666387407714
114	26	-0.004182053109424544
114	27	-0.015136728968563773
114	28	-0.046082921113082875
114	29	0.008706746327618475
114	30	0.04524115259230399
114	31	0.04568501807717951
114	32	0.09021747766704635
114	33	0.08775670153372772
114	34	0.0970544621076499
114	35	0.10196455858097409
114	36	0.13799128871757205
114	37	0.1825022839394137
114	38	0.19400007486281368
114	39	0.20396892369564767
114	40	0.18648418853065607
114	41	0.24463349299124815
114	42	0.25368945276296984
114	43	0.25493176807664497
114	44	0.27350019806721093
114	45	0.30669635065436296
114	46	0.2734717937313107
114	47	0.3824471950553985
114	48	0.3910231596072009
114	49	0.3999738660085157
114	50	0.4330282924548925
115	1	-0.0538692035851156
115	2	-0.035184410065362215
115	3	-0.05223578120702399
115	4	-0.03735786094439675
115	5	-0.04101179897720897
115	6	-0.04726903874941257
115	7	-0.059764150909771274
115	8	-0.04327612144358284
115	9	-0.05235549115758431
115	10	-0.03783286338844505
115	11	-0.05350923713880462
115	12	-0.05239948024986063
115	13	-0.05722196854314632
115	14	-0.03390263420276085
115	15	-0.04493912737785877
115	16	6.835101212345762E-4
115	17	-0.01980196362854514
115	18	-0.02510504016650117
115	19	-0.005160984160674786
115	20	0.00396091072189364
115	21	-0.009760964730830324
115	22	0.015565441005740865
115	23	-0.05110276950701831
115	24	-0.03432899008794976
115	25	-0.011237857932052009
115	26	-0.06409549046597363
115	27	-0.04635606137755843
115	28	-0.052002840601218736
115	29	9.760202926543116E-4
115	30	0.03185118348655891
115	31	0.0765303953415355
115	32	0.06538589585684917
115	33	0.06315464667929405
115	34	0.09900168053682279
115	35	0.12481324754619294
115	36	0.1364306070951642
115	37	0.15715277331118926
115	38	0.1537927693376986
115	39	0.18814347205371007
115	40	0.21865316203738416
115	41	0.26791896124219505
115	42	0.2827318950154236
115	43	0.24544351481064552
115	44	0.29662300027439414
115	45	0.3145632091288135
115	46	0.3972978660730746
115	47	0.3993905056613869
115	48	0.4104300721077152
115	49	0.4307382822803305
115	50	0.4885598671746436
116	1	-0.07763022983767343
116	2	-0.0805643148229862
116	3	-0.07399676867815685
116	4	-0.0861863162074954
116	5	-0.08597729197115456
116	6	-0.09028769133344383
116	7	-0.07669507928960684
116	8	-0.06401466030354186
116	9	-0.07513607868991556
116	10	-0.0665704474847928
116	11	-0.06125241887974653
116	12	-0.08628416190802568
116	13	-0.08855005087347934
116	14	-0.04954625522960043
116	15	-0.051276416148752446
116	16	-0.03644999339689292
116	17	-0.031146022790579764
116	18	-0.0347380597961191
116	19	-0.043267444185616455
116	20	-0.04844468957582393
116	21	-0.026467453252557505
116	22	-0.015504156565917526
116	23	-0.03193876043075505
116	24	-0.05920961095809929
116	25	-0.05106045899855616
116	26	-0.016950534881026158
116	27	-0.039993726270523666
116	28	-0.03532094218310533
116	29	0.03892636252444959
116	30	0.07520457304504191
116	31	0.1332820766313887
116	32	0.10747172380603587
116	33	0.14528978344350207
116	34	0.16222856720148826
116	35	0.19499451480061936
116	36	0.1421376794817064
116	37	0.1977217342908162
116	38	0.25623168522755435
116	39	0.2676702080196307
116	40	0.2979109099423738
116	41	0.31231543605325385
116	42	0.3231586425102577
116	43	0.320508353084044
116	44	0.3376648006434593
116	45	0.3800939223938471
116	46	0.4409211279540628
116	47	0.4271591215527124
116	48	0.5333041040246016
116	49	0.46380346082688106
116	50	0.5242262662490447
117	1	-0.08359318967225704
117	2	-0.07468969166848893
117	3	-0.05631626067995654
117	4	-0.05195025310923285
117	5	-0.07471376326176937
117	6	-0.06248742650858219
117	7	-0.06532865307069358
117	8	-0.07375329506741224
117	9	-0.06909581280920207
117	10	-0.060844436071761265
117	11	-0.0498154460065976
117	12	-0.03989954944053409
117	13	-0.05880169233503807
117	14	-0.03845504030164193
117	15	-0.05417977126976581
117	16	-0.020964368894333063
117	17	-0.01757423863597296
117	18	-0.012636788447089486
117	19	0.005731691904557579
117	20	-0.004519160891348406
117	21	0.031889283875379704
117	22	0.03506373590316514
117	23	-0.005226077180772393
117	24	-0.006133982461520304
117	25	0.014299071927389662
117	26	-0.019056880485258887
117	27	-0.026012930200195683
117	28	0.0024292734671789162
117	29	0.0736877982123545
117	30	0.052484915489834805
117	31	0.10699941470420526
117	32	0.10206161581206175
117	33	0.0794048308519848
117	34	0.14494598486565735
117	35	0.09587655884151047
117	36	0.18193749096272427
117	37	0.15390946795299243
117	38	0.22060774491804835
117	39	0.23224934883005233
117	40	0.23570394009128393
117	41	0.3023429888199658
117	42	0.34399292703060524
117	43	0.34820942236062535
117	44	0.369129511430458
117	45	0.41011265170034505
117	46	0.4747841141708875
117	47	0.4506202739288483
117	48	0.5331595066944814
117	49	0.5138014835575291
117	50	0.562451988433061
118	1	-0.07097995321062456
118	2	-0.05466729870660458
118	3	-0.07356307737737922
118	4	-0.07797242250833847
118	5	-0.0604393286038416
118	6	-0.06036768112525692
118	7	-0.08022250311529433
118	8	-0.05438772757496224
118	9	-0.0781773275615052
118	10	-0.08163851976453174
118	11	-0.07950692290668115
118	12	-0.05952477229289599
118	13	-0.04792566257447817
118	14	-0.056250902034129524
118	15	-0.053106239134848465
118	16	-0.029336919791681737
118	17	0.0144891666783451
118	18	0.0104179395621955
118	19	-0.006529986654634001
118	20	0.007207135305215218
118	21	0.018637037345826002
118	22	0.029181931201250677
118	23	-0.012136612335410746
118	24	-0.002054252284800384
118	25	-0.007209742940823105
118	26	-0.02656398455825837
118	27	-0.04139820051275933
118	28	0.006765881906602544
118	29	0.08660555629975164
118	30	0.09943927427178068
118	31	0.09878425417662129
118	32	0.12746461847156354
118	33	0.11700863228730896
118	34	0.12629654355561826
118	35	0.14499211925559763
118	36	0.16688773920163413
118	37	0.21167208886522104
118	38	0.2435178572047434
118	39	0.22814976563746825
118	40	0.22331859997670733
118	41	0.30233847442241313
118	42	0.3330896300504662
118	43	0.29227099264660694
118	44	0.3572576791942055
118	45	0.37663554567640745
118	46	0.43914928111550466
118	47	0.43627147764936325
118	48	0.4315882128224189
118	49	0.5056800177689382
118	50	0.5181219477639516
119	1	-0.08143963932563025
119	2	-0.08591201423717365
119	3	-0.08845714091279433
119	4	-0.0714232373951944
119	5	-0.0808659639004361
119	6	-0.07520839638004162
119	7	-0.07604047982722051
119	8	-0.07527910932819282
119	9	-0.07961127375900645
119	10	-0.07317053041577992
119	11	-0.09689285282132687
119	12	-0.07671505572302095
119	13	-0.09916308770457505
119	14	-0.08952126497531054
119	15	-0.08304469629313616
119	16	-0.09501256719209294
119	17	-0.0777058734117162
119	18	-0.07550464567623294
119	19	-0.07323094223968951
119	20	-0.064945219020899
119	21	-0.06024891668997867
119	22	-0.05535581707064807
119	23	-0.11401645301140206
119	24	-0.10859133602669857
119	25	-0.08646462816189171
119	26	-0.12297354519229486
119	27	-0.07357277230372716
119	28	-0.08761714525983422
119	29	-0.05142352299446884
119	30	0.019210293685581803
119	31	0.037732906370465
119	32	0.06131691305066056
119	33	0.04512300661531299
119	34	0.04558431130801921
119	35	0.07473924802295784
119	36	0.0507601338880832
119	37	0.1276542648151846
119	38	0.07546403537446622
119	39	0.13740973950299207
119	40	0.13510003070045684
119	41	0.21066119122053806
119	42	0.25261331844642854
119	43	0.22273427220082453
119	44	0.2939259202800386
119	45	0.24240683496149984
119	46	0.3274315432284567
119	47	0.29616006242025605
119	48	0.3440436970639098
119	49	0.41101186511031784
119	50	0.40688020734504704
120	1	-0.05514055866613887
120	2	-0.06843825423192129
120	3	-0.04759097621523978
120	4	-0.0671245774090039
120	5	-0.06977294691150604
120	6	-0.06345946339311581
120	7	-0.0875553356421861
120	8	-0.06304064717954105
120	9	-0.08397969525363891
120	10	-0.07076602170170787
120	11	-0.08684650888981338
120	12	-0.06742341406714303
120	13	-0.08352813062030862
120	14	-0.046989608052669496
120	15	-0.05708094086499926
120	16	-0.047433763100912074
120	17	-0.04149562946246732
120	18	-0.06239361813444333
120	19	-0.04243048339507283
120	20	-0.012657708414366198
120	21	-0.047525900919303865
120	22	-0.022919007322833806
120	23	-0.07110268755375415
120	24	-0.05665681625882968
120	25	-0.05764960339404013
120	26	-0.06391182387253025
120	27	-0.052884799465616084
120	28	-0.030256981346681755
120	29	0.06016391112388675
120	30	0.05517575494104676
120	31	0.1275448460264883
120	32	0.09998361986185245
120	33	0.14841847305157074
120	34	0.15075193205922943
120	35	0.1708210575889808
120	36	0.21444764299140218
120	37	0.20621090046554258
120	38	0.23490223667946708
120	39	0.2650667619765983
120	40	0.28579086934864506
120	41	0.32496670173268427
120	42	0.35267043776838847
120	43	0.391269062664513
120	44	0.3755465260492059
120	45	0.38734390057396695
120	46	0.45617191505508714
120	47	0.5242331817675208
120	48	0.4902912086404143
120	49	0.5575194687335809
120	50	0.593754037089127
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13193.0
3	528.0
4	796.0
5	843.0
6	1662.0
7	2885.0
8	3671.0
9	3779.0
10	5377.0
11	8200.0
12	7700.0
13	10150.0
14	14221.0
15	17377.0
16	17843.0
17	19646.0
18	25534.0
19	34138.0
20	45264.0
21	34490.0
22	46331.0
23	63729.0
24	72107.0
25	63791.0
26	87482.0
27	114711.0
28	134205.0
29	128902.0
30	138955.0
31	170999.0
32	197137.0
33	248148.0
34	351693.0
35	521886.0
36	838720.0
37	1606075.0
38	3598064.0
39	1.7427797E7
40	350289.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.44442574512997	28.37470490104722	22.555792637508134	29.62507671631468
2	25.3220238987589	28.31346663832333	24.770656989975677	21.59385247294209
3	23.5646021816447	26.275996830369607	25.359506420348055	24.799894567637637
4	26.365851798920207	25.170754059629076	23.886429711579083	24.576964429871637
5	25.96590351190687	26.95776316503921	25.17870376133707	21.89762956171685
6	25.955651002274422	25.74457755247502	22.85388573533895	25.445885709911604
7	25.030151370208277	25.42978331046266	25.401310064454346	24.138755254874713
8	24.66096394358928	25.060773844411543	25.51161992575386	24.766642286245318
9	24.214654901609705	24.82656293147373	25.7030205251806	25.25576164173596
10	24.571102103433144	26.204255601888853	24.012269717656647	25.21237257702136
11	24.791764762328118	25.792851325496578	24.592493405928284	24.82289050624702
12	24.72698792257608	25.429272494753548	25.106698050174813	24.73704153249556
13	24.662246761220295	25.61744565053289	25.044783402409493	24.67552418583733
14	24.499633309997254	25.849477064715202	25.15200929548373	24.49888032980381
15	24.2356324000642	25.59272973785165	25.276076971678634	24.89556089040551
16	24.38284948743238	25.595234626736367	25.17032298461067	24.851592901220577
17	24.47815256347377	25.569114916810065	25.341525707387053	24.611206812329108
18	24.307752010551713	25.419601050660884	25.248984819994973	25.023662118792426
19	24.530233062883532	25.466876098584855	25.163103455921785	24.83978738260982
20	24.536970697504323	25.46020467835704	25.34636835255547	24.656456271583167
21	24.43339330212484	25.35961314737395	25.285607281922886	24.921386268578328
22	24.575529967697605	25.370502798293316	25.12424950544067	24.929717728568406
23	24.46397458968066	25.50541052215279	25.399486263181785	24.63112862498476
24	24.528436248228367	25.34242752643918	25.250333042697836	24.87880318263461
25	24.65857337240138	25.326667878650085	25.07900093411528	24.935757814833252
26	24.627890958221847	25.51207096874302	25.280109299130675	24.579928773904456
27	24.60473295154492	25.228230360335	25.289199782858173	24.877836905261905
28	24.734222426619027	25.314115911537794	25.084294437246683	24.867367224596496
29	24.61699585813453	25.4966079396615	25.262403399666866	24.623992802537103
30	24.61304802898325	25.236787435128683	25.235999756273987	24.914164779614083
31	24.737321081563582	25.34122704356026	25.042863892224034	24.878587982652125
32	24.712746063840694	25.474623323770174	25.166654255588256	24.645976356800883
33	24.64581298755953	25.283242733053036	25.1302912782083	24.940653001179133
34	24.7591536031197	25.358705948282616	24.96790230537997	24.914238143217716
35	24.706115218250904	25.449212809364717	25.162117257407346	24.68255471497703
36	24.66679146656792	25.291863827385512	25.100205753032824	24.94113895301375
37	24.798506961099402	25.365735733767426	24.942765919691045	24.89299138544213
38	24.753078323662283	25.48774147719571	25.107914184633785	24.651266014508224
39	24.720411904731748	25.234974284774065	25.10627491253759	24.938338897956594
40	24.844533213202997	25.30088489433689	24.93722870035527	24.917353192104848
41	24.741162364064127	25.43891204188152	25.102942336245636	24.716983257808714
42	24.727667088928822	25.224358462439696	25.09363563008864	24.954338818542844
43	24.852848640097925	25.28567153501665	24.90125082166071	24.96022900322471
44	24.789443538051344	25.437265205628623	25.051041058481623	24.722250197838413
45	24.745138203892168	25.222086964627778	25.069683049350584	24.963091782129467
46	24.86944613668983	25.2729859226105	24.89043132922214	24.96713661147753
47	24.806299077724205	25.419975276043242	25.053546237910552	24.720179408321997
48	24.79483748702377	25.160504733322238	25.007677068466467	25.036980711187528
49	24.895038075568028	25.24937366407135	24.85329057222779	25.002297688132824
50	24.851682703505	25.360700262260856	25.0391409606447	24.748476073589448
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3697.0
1	2697.0
2	1697.0
3	1683.0
4	1669.0
5	1565.0
6	1461.0
7	1531.0
8	1601.0
9	1980.5
10	2360.0
11	3737.0
12	5114.0
13	6765.5
14	8417.0
15	12910.5
16	17404.0
17	25436.0
18	33468.0
19	45929.0
20	58390.0
21	77473.0
22	96556.0
23	126613.0
24	156670.0
25	192236.5
26	227803.0
27	270786.0
28	313769.0
29	358386.5
30	403004.0
31	462930.0
32	522856.0
33	594652.0
34	666448.0
35	749053.5
36	831659.0
37	918055.0
38	1004451.0
39	1099394.5
40	1194338.0
41	1277960.0
42	1361582.0
43	1443954.0
44	1526326.0
45	1620297.5
46	1714269.0
47	1794725.0
48	1875181.0
49	1942268.5
50	2009356.0
51	2039455.5
52	2069555.0
53	2051272.0
54	2032989.0
55	1963983.0
56	1894977.0
57	1794888.0
58	1694799.0
59	1568097.0
60	1441395.0
61	1274974.5
62	1108554.0
63	948184.0
64	787814.0
65	656344.0
66	524874.0
67	432590.5
68	340307.0
69	272615.0
70	204923.0
71	161574.5
72	118226.0
73	94910.0
74	71594.0
75	56994.0
76	42394.0
77	33965.5
78	25537.0
79	20428.5
80	15320.0
81	11582.5
82	7845.0
83	5922.5
84	4000.0
85	2919.0
86	1838.0
87	1382.5
88	927.0
89	672.5
90	418.0
91	286.5
92	155.0
93	106.5
94	58.0
95	52.5
96	47.0
97	50.5
98	54.0
99	113.0
100	172.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5108876017005698
2	0.0
3	0.0
4	3.8973346695767773E-4
5	3.1405706560667235E-4
6	2.9135414520137075E-4
7	0.0
8	3.0270560540402157E-5
9	0.0
10	0.0
11	1.2864988229670916E-4
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	8.70278615536562E-5
21	0.002584349106136834
22	9.270359165498161E-4
23	0.0
24	9.459550168875673E-5
25	0.012539579703861593
26	0.020500737125987357
27	0.026021330604543202
28	0.050956704849699476
29	0.06352655511410148
30	0.08179483840023417
31	0.12981529887751464
32	0.15363444620274358
33	0.16203074293263764
34	0.1518446993107923
35	0.15489445828523785
36	0.1895391148237281
37	0.18679584527475415
38	0.20916578951411133
39	0.21779668308819353
40	0.22393025541769246
41	0.2524375558066162
42	0.262956575594406
43	0.2883270891473305
44	0.28758546041409067
45	0.3098267547711512
46	0.32515879368486483
47	0.3294382941812642
48	0.3287496389289701
49	0.327765845711407
50	0.32914315621599527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.6428318E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.152399132327176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.50751140661765	41.972176934445606
2	11.404037430011286	12.351119732599395
3	4.169275265721652	6.7732877484569505
4	2.036678672539017	4.411641455185246
5	1.1677359617876626	3.161785194194873
6	0.7815769584446632	2.539456044379542
7	0.5264244158278548	1.9955001555238518
8	0.3924586663646522	1.700206267513558
9	0.30467030306322174	1.4848765069722
>10	1.5513369667145558	15.137355536539179
>50	0.10758642240730346	3.982082832664939
>100	0.05047301776058811	4.362115423183691
>500	1.9244416183469924E-4	0.06632638116556673
>1k	3.507591602554168E-5	0.03636949311407545
>5k	6.99266207620275E-6	0.02570029406125805
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.810876121590485E-5	6.810876121590485E-5	0.0	0.0	0.0
2	6.810876121590485E-5	1.2108224216160861E-4	0.0	7.567640135100538E-6	3.783820067550269E-6
3	7.946022141855565E-5	1.7783954317486265E-4	0.0	7.567640135100538E-6	7.567640135100538E-6
4	8.70278615536562E-5	2.2324538398546588E-4	0.0	7.567640135100538E-6	1.891910033775135E-5
5	1.2108224216160861E-4	2.572997645934183E-4	0.0	1.1351460202650808E-5	2.2702920405301617E-5
6	1.3243370236425942E-4	3.1405706560667235E-4	0.0	1.5135280270201077E-5	3.78382006755027E-5
7	1.475689826344605E-4	3.443276261470745E-4	0.0	1.5135280270201077E-5	4.5405840810603233E-5
8	1.627042629046616E-4	3.708143666199264E-4	0.0	1.5135280270201077E-5	4.5405840810603233E-5
9	1.9675864351261402E-4	3.9351728702522804E-4	0.0	1.891910033775135E-5	4.9189660878153505E-5
10	5.070318890517361E-4	4.275716676331804E-4	0.0	1.891910033775135E-5	5.297348094570377E-5
11	6.243303111457944E-4	4.6162604824113285E-4	0.0	1.891910033775135E-5	5.297348094570377E-5
12	7.15141992767001E-4	5.183833492543869E-4	0.0	1.891910033775135E-5	5.675730101325404E-5
13	8.324404148610593E-4	5.486539097947891E-4	0.0	1.891910033775135E-5	6.432494114835458E-5
14	9.573064770902181E-4	5.600053699974398E-4	0.0	1.891910033775135E-5	7.56764013510054E-5
15	0.0014567707260068536	5.864921104702917E-4	0.0	2.2702920405301617E-5	8.324404148610592E-5
16	0.001714070490600272	6.167626710106939E-4	0.0	2.2702920405301617E-5	1.0216314182385727E-4
17	0.0020583981167473465	6.810876121590485E-4	0.0	2.6486740472851885E-5	1.1729842209405836E-4
18	0.0025238079850560297	7.30277273037202E-4	0.0	2.6486740472851885E-5	1.1729842209405836E-4
19	0.0030875971751210198	7.681154737127047E-4	0.0	2.6486740472851885E-5	1.1729842209405836E-4
20	0.010409289005830791	8.173051345908581E-4	0.0	2.6486740472851885E-5	1.248660622291589E-4
21	0.011090376617989839	8.589271553339111E-4	0.0	2.6486740472851885E-5	1.248660622291589E-4
22	0.01165794962812238	8.778462556716625E-4	0.0	2.6486740472851885E-5	1.5513662276956105E-4
23	0.012236874098457572	8.967653560094139E-4	0.0	3.0270560540402153E-5	1.627042629046616E-4
24	0.012868772049738466	9.194682764147154E-4	0.0	3.4054380607952425E-5	1.7027190303976213E-4
25	0.01349688618095181	9.497388369551176E-4	0.0	3.4054380607952425E-5	1.7027190303976213E-4
26	0.014181757613178409	9.762255774279695E-4	0.0	3.4054380607952425E-5	1.740557231073124E-4
27	0.014942305446756014	0.0010216314182385727	0.0	3.4054380607952425E-5	1.740557231073124E-4
28	0.01842720372896981	0.0010556857988465252	0.0	3.78382006755027E-5	1.7783954317486265E-4
29	0.01934667200538453	0.0010746048991842766	0.0	4.162202074305296E-5	1.7783954317486265E-4
30	0.020285059382136993	0.0010935239995220278	0.0	4.162202074305296E-5	1.7783954317486265E-4
31	0.02305859949165134	0.001108659279792229	0.0	4.162202074305296E-5	1.7783954317486265E-4
32	0.024068879449687264	0.001154065120602832	0.0	4.162202074305296E-5	2.1567774385036537E-4
33	0.025158619629141742	0.001191903321278335	0.0	4.162202074305296E-5	2.459483043907675E-4
34	0.02614241284670481	0.0012297415219538375	0.0	4.162202074305296E-5	2.459483043907675E-4
35	0.02723215302615929	0.0012713635426968904	0.0	4.162202074305296E-5	2.6865122479606914E-4
36	0.02835594758622172	0.0013092017433723932	0.0	4.162202074305296E-5	2.8378650506627023E-4
37	0.02963109494898616	0.0013167693835074937	0.0	4.162202074305296E-5	2.9135414520137075E-4
38	0.03104245983418241	0.0013546075841829965	0.0	4.162202074305296E-5	2.95137965268921E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	7930	0.0	9.78059	12
CAACGCA	8300	0.0	9.3975315	13
ATCAACG	9160	0.0	8.707156	11
GTATCAA	9430	0.0	8.504452	9
AACGCAG	9565	0.0	8.361418	14
GGTATCA	10030	0.0	8.039523	8
ACGCAGA	10215	0.0	7.8725324	15
AGAGTAC	10670	0.0	7.558361	19
AGTGGTA	10800	0.0	7.4663773	5
TATCAAC	11235	0.0	7.392373	10
GTGGTAT	11055	0.0	7.2543907	6
CGCAGAG	11620	0.0	6.920712	16
AAGCAGT	13260	0.0	6.811965	1
TGGTATC	12160	0.0	6.70356	7
GAGTACT	6180	0.0	6.3654265	20
CAGAGTA	14150	0.0	5.8851647	18
GAGTACA	11195	0.0	5.6329207	17
GTACTTT	7340	0.0	5.5106006	22
GTACATG	11460	0.0	5.4265995	19
CAGTGGT	16255	0.0	5.3392296	4
>>END_MODULE