FastQCFastQC Report
Fri 27 May 2016
SRR522034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28244175
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC81300.010.5668189
CAACGCA86000.010.16816610
GTATCAA92200.09.4368126
AACGCAG98350.08.9136711
ATCAACG98050.08.82898
GGTATCA102050.08.8273825
AGTGGTA112100.08.17320252
ACGCAGA110600.08.10517312
TATCAAC111400.07.9089767
AGAGTAC110550.07.791093316
GTGGTAT118150.07.5315293
TGGTATC123150.07.52904464
GATCGGA37000.07.48535121
CGCAGAG122350.07.41658713
AATGCCG40900.07.091018713
CGAGATC42050.07.00187718
AGCGGTT43600.06.56355430
GAGTACA128400.06.502650317
GAGATCG44750.06.28562919
AGTACAT137050.06.23657618
Produced by FastQC (version 0.11.5)