FastQCFastQC Report
Fri 27 May 2016
SRR522033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28651201
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC90450.013.26258859
CAACGCA93950.012.7451210
ATCAACG100550.012.0396478
AACGCAG106000.011.48280811
GTATCAA109700.011.1957696
ACGCAGA114300.010.68741812
GGTATCA118050.010.3294335
AGTGGTA119350.010.2907372
AGAGTAC122250.09.92084916
TATCAAC125200.09.9149017
CGCAGAG127400.09.76094813
GTGGTAT125250.09.7358163
TGGTATC134850.09.2219474
GAGTACA121650.07.657947517
CAGAGTA166400.07.59225715
CAGTGGT180400.07.3936221
GTACATG125950.07.24028319
AGTACAT129300.07.17089418
GCAGAGT192450.06.89565314
ACATGGG134800.06.733546321
Produced by FastQC (version 0.11.5)