##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522033_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28651201
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.94977690464005	40.0	39.0	40.0	38.0	40.0
2	38.88984070859717	40.0	39.0	40.0	38.0	40.0
3	38.87871513658363	40.0	39.0	40.0	38.0	40.0
4	38.872581885834386	40.0	39.0	40.0	38.0	40.0
5	38.87096764285727	40.0	39.0	40.0	38.0	40.0
6	38.94734807102851	40.0	39.0	40.0	38.0	40.0
7	38.925656589404404	40.0	39.0	40.0	38.0	40.0
8	38.908267754639674	40.0	39.0	40.0	38.0	40.0
9	38.88486946847359	40.0	39.0	40.0	38.0	40.0
10	38.870323411573565	40.0	39.0	40.0	38.0	40.0
11	38.9215850672368	40.0	39.0	40.0	38.0	40.0
12	38.88085089347563	40.0	39.0	40.0	37.0	40.0
13	38.85158088137387	40.0	39.0	40.0	37.0	40.0
14	38.82578716333741	40.0	39.0	40.0	37.0	40.0
15	38.797667888337386	40.0	39.0	40.0	37.0	40.0
16	38.75888731505531	40.0	39.0	40.0	36.0	40.0
17	38.70104816897553	40.0	39.0	40.0	36.0	40.0
18	38.64738951082714	40.0	39.0	40.0	36.0	40.0
19	38.60222972851993	40.0	39.0	40.0	36.0	40.0
20	38.54635821374469	40.0	38.0	40.0	36.0	40.0
21	38.460700513043065	40.0	38.0	40.0	35.0	40.0
22	38.38178095919958	40.0	38.0	40.0	35.0	40.0
23	38.5365327966531	40.0	38.0	40.0	36.0	40.0
24	38.49915425883892	40.0	38.0	40.0	36.0	40.0
25	38.45592193500021	40.0	38.0	40.0	36.0	40.0
26	38.417256854258916	40.0	39.0	40.0	36.0	40.0
27	38.35240072484221	40.0	38.0	40.0	35.0	40.0
28	38.280240119777176	40.0	38.0	40.0	35.0	40.0
29	38.36809462193225	40.0	39.0	40.0	36.0	40.0
30	38.32256581495484	40.0	39.0	40.0	35.0	40.0
31	38.26647141248983	40.0	39.0	40.0	35.0	40.0
32	38.19757402141711	40.0	39.0	40.0	35.0	40.0
33	38.14377208829745	40.0	39.0	40.0	35.0	40.0
34	38.08203303589263	40.0	38.0	40.0	35.0	40.0
35	38.02709062702119	40.0	38.0	40.0	35.0	40.0
36	37.90335951362039	40.0	38.0	40.0	35.0	40.0
37	37.82099923141093	40.0	38.0	40.0	35.0	40.0
38	37.74843717720594	40.0	38.0	40.0	35.0	40.0
39	37.67820448434256	40.0	38.0	40.0	34.0	40.0
40	37.59862621465676	40.0	38.0	40.0	34.0	40.0
41	37.42019477647726	40.0	38.0	40.0	34.0	40.0
42	37.31681394437881	40.0	38.0	40.0	33.0	40.0
43	37.24747482662245	40.0	38.0	40.0	33.0	40.0
44	37.15772864111351	39.0	38.0	40.0	33.0	40.0
45	37.03237539675911	39.0	38.0	40.0	33.0	40.0
46	36.84385349151681	39.0	38.0	40.0	33.0	40.0
47	36.73302731707477	39.0	37.0	40.0	33.0	40.0
48	36.62757906727889	39.0	37.0	40.0	32.0	40.0
49	36.53613480286568	39.0	37.0	40.0	32.0	40.0
50	36.426553916535646	39.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.02963192858231878
1	2	0.024092404783829124
1	3	0.02238074073862606
1	4	0.03164862016075176
1	5	-0.006355304107927395
1	6	-0.0013071251841978437
1	7	0.009993498048949334
1	8	-0.045789433311696826
1	9	-0.0055116363400173896
1	10	-0.027098453107569753
1	11	-0.013337609570172049
1	12	-0.005645346792249484
1	13	0.010568309737905679
1	14	-0.025797234720975837
1	15	-0.019260031763629115
1	16	-0.01885093666858495
1	17	-0.029062251248056725
1	18	-0.043642847194796275
1	19	-0.07156914706526862
1	20	-0.05983766219898001
1	21	-0.03989559173600554
1	22	-0.0695786424442062
1	23	-0.10820281306759227
1	24	-0.09182014662020777
1	25	-0.11171632665855213
1	26	-0.15198001538413308
1	27	-0.20320780094103696
1	28	-0.15517173317932986
1	29	-0.11615898678374492
1	30	-0.14408413967619538
1	31	-0.10353851263493397
1	32	-0.08773487192032547
1	33	-0.10033363362038017
1	34	-0.10321471899414547
1	35	-0.07350057992852044
1	36	-0.03262435077336079
1	37	-0.12276241060862247
1	38	-0.10939707314279445
1	39	-0.143792563489626
1	40	-0.08385841506108704
1	41	-0.022094747652687374
1	42	-0.09411154156314439
1	43	0.01351779400008013
1	44	-0.049024271336470804
1	45	-0.02445193043077154
1	46	-0.0406357416508456
1	47	-1.966754599393994E-4
1	48	0.04565884128523834
1	49	0.032786284677321476
1	50	0.04104855447982203
2	1	0.00892205656582945
2	2	0.06802545741912525
2	3	0.056631869065192575
2	4	0.07499537427564462
2	5	0.06356846527441462
2	6	0.04223966937541235
2	7	0.04271263759665089
2	8	0.054105967818578904
2	9	0.049324338785027066
2	10	0.01642499540380271
2	11	0.042967615111614066
2	12	0.03700811045784036
2	13	0.026623336153591026
2	14	0.012407700958675605
2	15	0.027741650037597765
2	16	0.010987903517793995
2	17	0.00966146294977932
2	18	-0.023229600309477405
2	19	0.019335877832148185
2	20	0.00841772706314714
2	21	-0.003932452992835067
2	22	-0.02501777316574305
2	23	-0.017778390001573996
2	24	-0.03586393166170865
2	25	-0.04737439059341142
2	26	-0.06532904445766263
2	27	-0.08111921227933294
2	28	-0.09530652216444224
2	29	-0.02449910821918877
2	30	-0.004300475710849128
2	31	0.03602346962276215
2	32	0.006768659509077679
2	33	-0.016965884726666047
2	34	0.011697762580261895
2	35	-0.033657461965958646
2	36	0.014366825519076087
2	37	-0.034174644593129244
2	38	0.037486385700972846
2	39	-0.019929734779481123
2	40	0.0018251760819651963
2	41	0.0836835439308814
2	42	0.0030618838017559824
2	43	0.07097048397925221
2	44	-0.011253258166981084
2	45	0.04908423501247228
2	46	0.014645943275944262
2	47	0.0644473596333981
2	48	0.028450975452962268
2	49	0.03266601285465498
2	50	0.01789295389585277
3	1	0.01209042367945301
3	2	0.04862534580090738
3	3	0.04573225647801138
3	4	0.03759080297233908
3	5	0.03409456232309083
3	6	0.0391301700554294
3	7	0.03504501853954167
3	8	0.02317132897340457
3	9	0.005881358865480024
3	10	0.036560766488385354
3	11	0.031783290261728325
3	12	0.02301265510681816
3	13	0.03632492944147003
3	14	0.005980761735877138
3	15	-0.007545220334009173
3	16	-0.00960339912165864
3	17	0.0022784078084754356
3	18	-0.02338041149386072
3	19	-0.018035241965890236
3	20	-0.004847344498074335
3	21	-0.03908315531508322
3	22	-0.0323027479635698
3	23	-0.014884755581206832
3	24	-0.07040227095312446
3	25	-0.06566798126239348
3	26	-0.06868309723894583
3	27	-0.09353183935927945
3	28	-0.13545168861975299
3	29	-0.03577223669054064
3	30	-0.026953150668759918
3	31	-0.026549696813439994
3	32	-0.019685926073627513
3	33	-0.00947731749440095
3	34	-0.035867601910929636
3	35	-0.0284884064968125
3	36	0.004551343581432832
3	37	0.005223755101894767
3	38	-0.03971531295281494
3	39	-0.02827827761852575
3	40	0.0028557434196514464
3	41	0.005732912364059928
3	42	-0.05629067580895253
3	43	0.023935423640352838
3	44	0.017077169614729826
3	45	-0.023635484749689795
3	46	-0.08729580800939374
3	47	-0.07157677451145616
3	48	0.017713546352140952
3	49	-0.04343854815122228
3	50	-0.03587209821392179
4	1	0.03280660163786564
4	2	0.07403642065373361
4	3	0.08550311884852135
4	4	0.08433431774755462
4	5	0.08065601006543943
4	6	0.07200962736907002
4	7	0.07348436309653295
4	8	0.05180204017742085
4	9	0.06865273224564561
4	10	0.037865622966783974
4	11	0.05435459996738956
4	12	0.04160307353938464
4	13	0.02067221361335214
4	14	-5.174815208164318E-4
4	15	0.01665561758438372
4	16	0.039191501172417986
4	17	0.04104695726991281
4	18	0.030240490628003158
4	19	0.02998234569665925
4	20	0.008560644867301903
4	21	0.01762656673880514
4	22	0.013113750111109823
4	23	-0.012207336501205646
4	24	-0.037683140107233726
4	25	-0.01736066414107995
4	26	-0.0472542848302453
4	27	-0.045447019144816636
4	28	-0.09704678799561606
4	29	0.014610685114028854
4	30	0.027354938367928128
4	31	0.04462743438403294
4	32	0.0440821400172311
4	33	0.05464317160845411
4	34	0.01645859564387564
4	35	0.031163370645977295
4	36	-0.006806109779127212
4	37	-0.02806069892276497
4	38	-0.01822838096097712
4	39	-0.0178820544932492
4	40	-4.974691784838114E-4
4	41	0.09953315030929843
4	42	0.05609958859754016
4	43	0.07610878443951918
4	44	0.06895661021349753
4	45	0.12522738409894174
4	46	0.10054174641227576
4	47	0.07920350580920399
4	48	0.1115905188751043
4	49	0.12731752877132152
4	50	0.09778683719061831
5	1	-0.0036932345982094716
5	2	0.06209970600698966
5	3	-0.032036371635278726
5	4	0.0533796879745978
5	5	0.04796414410653682
5	6	0.054096705370660914
5	7	0.04799957010414602
5	8	0.0568288271546038
5	9	0.04836193911188502
5	10	0.05517689621045463
5	11	0.025517335380030204
5	12	0.06174626673118411
5	13	0.053409053462935674
5	14	0.009773482818111745
5	15	0.03548246756560047
5	16	0.02842780146838919
5	17	0.042321971849744955
5	18	0.019548438882893038
5	19	0.03987946756513594
5	20	0.07379804731695572
5	21	0.032404325511919296
5	22	0.045097934217892544
5	23	0.010739186566574688
5	24	-0.006315526940660732
5	25	0.0166802156153949
5	26	0.010931645344868457
5	27	-0.017459523612743055
5	28	-0.02088991310662891
5	29	0.003683867747966474
5	30	0.031020809678288686
5	31	0.0548026870855054
5	32	0.05843750595006014
5	33	0.04640678105149476
5	34	0.08596753985524686
5	35	0.05808354318696729
5	36	0.0775234025851006
5	37	0.09915232915447092
5	38	0.09713709796839254
5	39	0.07045585568523194
5	40	0.11015671200678412
5	41	0.10459586493307427
5	42	0.16490977262386508
5	43	0.13325192617859472
5	44	0.10181045864255367
5	45	0.08107025662093292
5	46	0.11022942390869872
5	47	0.15270271270343017
5	48	0.125965383051863
5	49	0.145203600246802
5	50	0.17997053399040652
6	1	-1.4425270387619094E-4
6	2	0.06304929612904431
6	3	0.07677498578956943
6	4	0.08236508640895579
6	5	0.06969847540943874
6	6	0.06281387414534834
6	7	0.05007472450861883
6	8	0.043848573547329295
6	9	0.05866485791185028
6	10	0.042869622522324846
6	11	0.02370556655606748
6	12	0.01644794761047308
6	13	0.029401794083184996
6	14	0.019280554954988816
6	15	0.012012332336965414
6	16	6.6835887017902E-4
6	17	0.02175811964765728
6	18	0.034774482308264965
6	19	0.012586051769901019
6	20	0.028132185934218512
6	21	0.03641933865448266
6	22	0.03196696436774005
6	23	-0.002270276786518366
6	24	0.028704220021054994
6	25	0.02032204102687274
6	26	9.703174302657658E-4
6	27	-0.0485380574927845
6	28	-0.03930049450441686
6	29	0.00224937184096774
6	30	0.03643745054026937
6	31	0.08453587457191958
6	32	0.0866882332687311
6	33	0.10301765999305701
6	34	0.039360627678064475
6	35	0.0688015062035845
6	36	0.09761656491566129
6	37	0.06936271809421868
6	38	0.04189345383749554
6	39	0.07841141512178496
6	40	0.09002025292926419
6	41	0.09164040249189753
6	42	0.1150162194988198
6	43	0.04660239986723269
6	44	0.10928975830780274
6	45	0.05548155021909196
6	46	0.08087567862184386
6	47	0.10779778053538536
6	48	0.1554630789092286
6	49	0.15449389081704368
6	50	0.10910594809605101
7	1	0.008903425713626234
7	2	0.07748967512056026
7	3	0.06667137703256998
7	4	0.08600457835079567
7	5	0.0851432488081727
7	6	0.03299874028147798
7	7	0.03965303569047052
7	8	0.03423410754034251
7	9	-0.003212857369639721
7	10	-0.07203400923204839
7	11	-0.011351715489112735
7	12	-0.00642960997680575
7	13	-0.014691735881974921
7	14	-0.03269280470042446
7	15	-0.02661142643123071
7	16	0.009131861704332778
7	17	-0.007155835227678153
7	18	0.008731789453882755
7	19	-0.0023582739911631734
7	20	0.021331291132618446
7	21	0.00267764047947594
7	22	-0.02397069639045668
7	23	0.009267922213524571
7	24	-0.021922619849405578
7	25	-0.04735165426527743
7	26	-0.04685540455548676
7	27	-0.02732416664976256
7	28	-0.08937655307077108
7	29	0.011579726282569425
7	30	-0.01513584170213278
7	31	0.04392358606573765
7	32	0.04837923487244922
7	33	0.028579263484068917
7	34	0.019739182787091636
7	35	0.012702867434079224
7	36	0.08861284995013818
7	37	0.06797241429994472
7	38	0.0430768602434739
7	39	0.015352751798786812
7	40	0.06877736415219715
7	41	0.07551211405260716
7	42	0.049468058416444194
7	43	0.07871242592445782
7	44	0.07388276046032871
7	45	0.06620481037355574
7	46	0.08049127655313981
7	47	0.06083684656648103
7	48	0.0037303662178160835
7	49	0.019531996901683613
7	50	0.004674518268934946
8	1	0.04558961399135342
8	2	0.09620746576293016
8	3	0.10864480980855973
8	4	0.12027814324587638
8	5	0.10697829277231818
8	6	0.07744432999569995
8	7	0.07010147179147452
8	8	0.09539237462264794
8	9	0.07275817363954928
8	10	0.04970282092422451
8	11	0.048232431876932935
8	12	-0.03177433377073413
8	13	0.036358465663823836
8	14	0.03520982482452695
8	15	0.02148653216573848
8	16	0.010056597802140743
8	17	0.0021680817963058985
8	18	0.0058992338201449
8	19	0.01842102633305842
8	20	0.020763419170073405
8	21	0.03160531246611953
8	22	0.03565488421074292
8	23	-0.009153579550911672
8	24	0.027257576535816952
8	25	-0.0038158675834409905
8	26	-0.024185000094377074
8	27	-0.040145732751625474
8	28	-0.03242332280545668
8	29	0.028368604434106715
8	30	0.031360220472599565
8	31	0.06388026114839107
8	32	0.04614876478099461
8	33	0.03227148197446894
8	34	0.03674406652571349
8	35	0.0278119443244762
8	36	0.06580417614171097
8	37	0.023881484889557214
8	38	0.04058607912324419
8	39	0.09164543983293072
8	40	0.061603796506545905
8	41	0.11958756245041258
8	42	0.1227324652960391
8	43	0.1402786246129395
8	44	0.15354392655643068
8	45	0.10700295670805815
8	46	0.14816686929106737
8	47	0.18990278910472114
8	48	0.20028102921942548
8	49	0.14478413689425906
8	50	0.15951504613641987
9	1	0.018847201807545844
9	2	0.09459416047141644
9	3	0.08819421849120346
9	4	0.08680505705980579
9	5	0.09775961340353234
9	6	0.06579087101795267
9	7	0.06357868038011816
9	8	0.07790304167601647
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112	49	0.2501700997401315
112	50	0.35563592730319726
113	1	-0.1645839862440397
113	2	-0.24410763700865346
113	3	-0.21899241633541067
113	4	-0.1997118303740919
113	5	-0.23866485135528848
113	6	-0.21412016539950685
113	7	-0.2136297303767094
113	8	-0.1975816549894489
113	9	-0.18607687106110404
113	10	-0.18155507704413054
113	11	-0.17440927257446504
113	12	-0.16787352401068034
113	13	-0.1607908236242821
113	14	-0.14925325030915815
113	15	-0.14870922300130474
113	16	-0.1341310578667816
113	17	-0.12014114153638644
113	18	-0.1502294028329274
113	19	-0.14660558674414403
113	20	-0.10133950967382077
113	21	-0.14496892941490103
113	22	-0.10467037362104747
113	23	-0.17499174353025637
113	24	-0.1589008777460137
113	25	-0.15130551298000938
113	26	-0.15658317048756487
113	27	-0.13274376421766476
113	28	-0.1438685293027504
113	29	-0.09197921571773549
113	30	-0.08326663606512597
113	31	-0.018366211917665964
113	32	-0.02080762402385261
113	33	-0.008589719907888593
113	34	0.028590866664757186
113	35	0.047960007770804225
113	36	0.040206056437831705
113	37	0.07002075633677407
113	38	0.08341747535536825
113	39	0.09010399657265111
113	40	0.08805101266801785
113	41	0.15740951936876257
113	42	0.171818886429854
113	43	0.13864628985756156
113	44	0.2401222488444219
113	45	0.21109001116183634
113	46	0.1664888750130018
113	47	0.18753158106350298
113	48	0.19211753790913377
113	49	0.28472574989378074
113	50	0.3098079655125332
114	1	-0.1389120008877498
114	2	-0.18821892143212438
114	3	-0.20639356432311473
114	4	-0.19253457909624672
114	5	-0.17192878943358636
114	6	-0.1584882443392175
114	7	-0.14450054634335174
114	8	-0.19009610957726153
114	9	-0.16227979281891436
114	10	-0.1679225616585711
114	11	-0.15833220913940949
114	12	-0.18077045893896582
114	13	-0.14670679266522058
114	14	-0.16566163801889644
114	15	-0.17986504724809294
114	16	-0.1575113918432578
114	17	-0.16039970351223332
114	18	-0.15858084164748476
114	19	-0.13381281982339033
114	20	-0.10562552410859638
114	21	-0.13166008133495666
114	22	-0.10300465490160349
114	23	-0.1682915748790137
114	24	-0.1426125440875481
114	25	-0.1237250858745611
114	26	-0.11381920922888611
114	27	-0.1367403307749555
114	28	-0.09713345585240774
114	29	-0.10131435546009016
114	30	-0.06991119027198067
114	31	0.0037697215605518863
114	32	0.014107533652861548
114	33	-0.0381138556061984
114	34	0.031250559652953314
114	35	-0.015748248763493677
114	36	0.007916379544738561
114	37	0.04834011346227385
114	38	0.06320224488977289
114	39	0.08615608355252391
114	40	0.06041822489662252
114	41	0.11852004657578874
114	42	0.18039777189848394
114	43	0.09979614210190135
114	44	0.16874456839779128
114	45	0.1835572913077499
114	46	0.27207352440234445
114	47	0.27059501562818156
114	48	0.32757608756448775
114	49	0.3413576017668518
114	50	0.38665874631173125
115	1	-0.14302256521352064
115	2	-0.22570750577329335
115	3	-0.22074883749972685
115	4	-0.1911791997306338
115	5	-0.20670160206662302
115	6	-0.19456527924965883
115	7	-0.2015867542896359
115	8	-0.21761803477591002
115	9	-0.1719613687395949
115	10	-0.18247014388255423
115	11	-0.196422363496346
115	12	-0.1880832583431129
115	13	-0.1719564624662695
115	14	-0.1828957922131238
115	15	-0.18320694659094983
115	16	-0.16871956842461344
115	17	-0.17580233079040397
115	18	-0.13880748251044395
115	19	-0.15017391890612686
115	20	-0.15033776126731624
115	21	-0.11532960574373874
115	22	-0.13024581076670216
115	23	-0.13623464478522607
115	24	-0.13078973646898362
115	25	-0.1250739219412509
115	26	-0.11114581337392337
115	27	-0.114854475039742
115	28	-0.11813657154116441
115	29	-0.06524292604677129
115	30	-0.04321044269432406
115	31	0.0033229826220235736
115	32	-0.013604611852784387
115	33	-0.010237339021365699
115	34	0.024838246062955704
115	35	0.011038388670868926
115	36	0.04429814888746364
115	37	0.06985167971076578
115	38	0.07093314191292421
115	39	0.08128974049312632
115	40	0.06964589195772675
115	41	0.13659979960244328
115	42	0.1102499713082068
115	43	0.16642804275073075
115	44	0.14638141843629882
115	45	0.19308071974300844
115	46	0.20917878097879594
115	47	0.15660641315120927
115	48	0.15306690627812003
115	49	0.19942896368401364
115	50	0.3267821091085352
116	1	-0.17580701088002826
116	2	-0.29554845033730714
116	3	-0.21707130428914923
116	4	-0.19915918189886384
116	5	-0.20584415100506703
116	6	-0.18132534902651543
116	7	-0.18606143536140252
116	8	-0.16974628633812472
116	9	-0.17006595038176897
116	10	-0.16591871005326198
116	11	-0.16334692109210636
116	12	-0.1606129413038886
116	13	-0.14856765444585562
116	14	-0.16712372183765467
116	15	-0.16859596010905875
116	16	-0.11928142041239198
116	17	-0.12192276627732213
116	18	-0.13440245745554336
116	19	-0.1359273651364532
116	20	-0.131269869694151
116	21	-0.11069123218123167
116	22	-0.13554769936266808
116	23	-0.10981069663348109
116	24	-0.13052060325019
116	25	-0.14237232211564788
116	26	-0.0733207332050938
116	27	-0.08763236729657109
116	28	-0.07155394910084567
116	29	-0.031149787186429023
116	30	-0.0445769273751111
116	31	0.030659257624620295
116	32	0.03753668279635036
116	33	0.021795556081762868
116	34	0.006787267246821216
116	35	0.06562817415448308
116	36	0.08490632566489609
116	37	0.0678976018981956
116	38	0.07649858513853047
116	39	0.0901532169702719
116	40	0.10592103865966607
116	41	0.1318425987203895
116	42	0.1719240931019712
116	43	0.15096032570482976
116	44	0.22918770360516305
116	45	0.2134006337970078
116	46	0.2543483729118279
116	47	0.2591307062004944
116	48	0.2768275272650911
116	49	0.3159765868818667
116	50	0.3707877941459259
117	1	-0.17660063621249122
117	2	-0.2658561182806025
117	3	-0.2601549499039919
117	4	-0.2547610768225681
117	5	-0.25429577957924465
117	6	-0.23849845406462578
117	7	-0.22643040876107534
117	8	-0.21137915327335577
117	9	-0.22992444726506278
117	10	-0.22073996485205782
117	11	-0.20209758117348997
117	12	-0.19512685915488248
117	13	-0.2281629029801806
117	14	-0.193226025922705
117	15	-0.17200546823449514
117	16	-0.16701504339224016
117	17	-0.18127076627528282
117	18	-0.1953089369722747
117	19	-0.15707084760617107
117	20	-0.16009932197810173
117	21	-0.176327891815518
117	22	-0.14832998122518148
117	23	-0.171262300255691
117	24	-0.17250788996815913
117	25	-0.17044965080893348
117	26	-0.19010638001864777
117	27	-0.2286761641101549
117	28	-0.16414433505686077
117	29	-0.12397899911672994
117	30	-0.09697249447986422
117	31	-0.05872072672183748
117	32	-0.030966143141270663
117	33	-0.02025181904764395
117	34	-0.02206000787101914
117	35	0.009401970820391625
117	36	0.029261401327921988
117	37	0.022881451864563473
117	38	0.03953555529750474
117	39	0.0566961931696639
117	40	0.047740688894187144
117	41	0.051058057940984725
117	42	0.15286511836303873
117	43	0.1113284537929573
117	44	0.11890408121092833
117	45	0.15716679210552797
117	46	0.27781798981764894
117	47	0.279208128590966
117	48	0.27340096014149395
117	49	0.2954884754759206
117	50	0.2935790230710609
118	1	-0.18373539997256216
118	2	-0.27405111938111304
118	3	-0.24328312080871228
118	4	-0.26810949430819875
118	5	-0.25417318308107184
118	6	-0.22565879945948808
118	7	-0.20813927975473945
118	8	-0.23082794229938486
118	9	-0.23137504662432207
118	10	-0.20319230263134358
118	11	-0.17627612379901336
118	12	-0.18698917044878982
118	13	-0.18773131631549234
118	14	-0.1986880733278582
118	15	-0.16651866657406345
118	16	-0.19731878313925932
118	17	-0.1560246145760047
118	18	-0.17608578927655572
118	19	-0.1281180188954636
118	20	-0.1467222063388931
118	21	-0.13429407548890282
118	22	-0.11102884285669745
118	23	-0.17504584132757373
118	24	-0.1761545693778075
118	25	-0.23485577566621174
118	26	-0.21579508745465148
118	27	-0.20742039836160586
118	28	-0.183296660408665
118	29	-0.11451079016765675
118	30	-0.1249833188863505
118	31	-0.0252444144882773
118	32	-0.02494062175581746
118	33	-0.010036367240104482
118	34	-0.024027257907377475
118	35	0.028601622951775596
118	36	0.04886886932591494
118	37	0.06915705668024685
118	38	0.05708657402741579
118	39	0.08658666272263815
118	40	0.105272850755469
118	41	0.15526801833165393
118	42	0.1016194464767537
118	43	0.1581309466387637
118	44	0.17526205224475433
118	45	0.21643838094188084
118	46	0.24070296988376327
118	47	0.21757985073946884
118	48	0.21689228184502696
118	49	0.27412639229876845
118	50	0.2974802937027903
119	1	-0.2026648683279717
119	2	-0.23334179440666247
119	3	-0.2238587731709103
119	4	-0.2345744300061554
119	5	-0.24399524224000402
119	6	-0.2146932681885474
119	7	-0.2264944307223402
119	8	-0.21347574349672982
119	9	-0.20848304026448972
119	10	-0.21833445413093955
119	11	-0.20326933515092094
119	12	-0.18514266009788827
119	13	-0.18590133523866825
119	14	-0.19707428892115786
119	15	-0.17567564721939277
119	16	-0.18172485316797804
119	17	-0.17125740938879375
119	18	-0.17620887849077604
119	19	-0.15404869814794608
119	20	-0.12311157708123233
119	21	-0.11792323303947683
119	22	-0.12673291648280838
119	23	-0.19106697882502033
119	24	-0.17270070334894427
119	25	-0.22720407446830393
119	26	-0.17580867119718135
119	27	-0.22257013882435217
119	28	-0.1390851814251448
119	29	-0.14321087440636404
119	30	-0.051804524944977004
119	31	-0.056720397501827335
119	32	-0.027790703695877994
119	33	-0.015238701101502272
119	34	-0.013105989036965582
119	35	0.020511080709098906
119	36	0.02248891234062711
119	37	0.06244312849746336
119	38	-0.012168231239940042
119	39	0.03298292219413668
119	40	0.07362191767611392
119	41	0.1313768508999189
119	42	0.11917770209716849
119	43	0.14666731968793556
119	44	0.19818054746095726
119	45	0.26048119355903765
119	46	0.24863030265696295
119	47	0.27850297340964403
119	48	0.27862466937081365
119	49	0.28371029041166906
119	50	0.3197835041421513
120	1	-0.12849069819269232
120	2	-0.1853399467719825
120	3	-0.17568167508009225
120	4	-0.18571105014031275
120	5	-0.18723606554635097
120	6	-0.17827081807696743
120	7	-0.1691017496649323
120	8	-0.17470242067068398
120	9	-0.18341534079506516
120	10	-0.17005427050722233
120	11	-0.13571765097843524
120	12	-0.14277751760047153
120	13	-0.1686120443219039
120	14	-0.13125839575437226
120	15	-0.15558560772245755
120	16	-0.12742028593370947
120	17	-0.1230153256549471
120	18	-0.1173687551336755
120	19	-0.1110973771231798
120	20	-0.11817744199338875
120	21	-0.07345077112102416
120	22	-0.06900156264971002
120	23	-0.10904838052884713
120	24	-0.15552694807558964
120	25	-0.10729545466459456
120	26	-0.13445508423103547
120	27	-0.16449963909009568
120	28	-0.11177175207250656
120	29	-0.07077902068697739
120	30	-0.055677245583318324
120	31	-0.01242310364126098
120	32	0.00978263473799501
120	33	0.02656447732346834
120	34	0.0633652052242013
120	35	0.05433981715896863
120	36	0.10995148093983431
120	37	0.14564497105002516
120	38	0.11743691419616908
120	39	0.12428677782644115
120	40	0.21501232603016973
120	41	0.24624897837713888
120	42	0.2281235796274217
120	43	0.25618859220618617
120	44	0.23028062965720153
120	45	0.3466269742527359
120	46	0.3682605705235886
120	47	0.3797140151160292
120	48	0.3875532671896096
120	49	0.45490033066347735
120	50	0.44526073091245877
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17702.0
3	662.0
4	1001.0
5	1149.0
6	2108.0
7	3552.0
8	4365.0
9	4647.0
10	6324.0
11	9724.0
12	9215.0
13	11816.0
14	16554.0
15	19795.0
16	20612.0
17	22061.0
18	28706.0
19	37708.0
20	48040.0
21	38752.0
22	51314.0
23	69246.0
24	78349.0
25	74274.0
26	100276.0
27	132161.0
28	154028.0
29	152713.0
30	172032.0
31	215107.0
32	261555.0
33	338158.0
34	469916.0
35	682777.0
36	1110768.0
37	2016934.0
38	4254562.0
39	1.774377E7
40	268768.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.253925100203794	29.737203630650598	21.47384696618718	30.53502430295843
2	24.547798188285373	28.244596099130366	26.152334766001605	21.055270946582656
3	22.98201042252993	26.96452061468558	25.531254344276878	24.522214618507615
4	24.68011918292258	26.174083616452748	24.98477788486437	24.161019315760296
5	25.28745156175968	27.345796463844852	25.642487611764615	21.72426436263085
6	24.91485287877232	26.26862771062984	24.06987358695149	24.74664582364635
7	24.441596706539457	26.065315726206386	25.80020293041119	23.692884636842972
8	23.40171597114967	25.63830789383516	26.463348927920283	24.496627207094885
9	23.351886714975752	25.255342699246707	26.78055624963156	24.61221433614598
10	23.978251382900144	26.42752043797396	24.951505523276317	24.642722655849575
11	24.12164502283866	26.177300560629202	25.325004700501037	24.3760497160311
12	23.956461720400483	26.10878685329805	25.568907216140786	24.365844210160684
13	24.141898973100638	26.122758344405877	25.54158200907529	24.193760673418193
14	23.997643240155973	26.212143777149166	25.80636672089243	23.983846261802427
15	23.737409820970505	26.056621500788047	25.701215805927298	24.504752872314146
16	24.04525031952413	26.14338226170694	25.73039433844326	24.080973080325673
17	24.08450870872743	26.090658468383225	25.82642172661453	23.998411096274815
18	23.87261183222302	26.089314720175256	25.573332859589375	24.464740588012347
19	24.106884110641026	26.062712737740203	25.695684237239647	24.134718914379132
20	24.056459681249738	25.990873004460113	25.84479821427923	24.107869100010916
21	23.98894844048199	25.985569170672324	25.698167194626677	24.32731519421901
22	23.997392634835617	25.9485083074862	25.61916720538857	24.43493185228961
23	23.930404161836734	26.028183954345224	25.94287163439879	24.098540249419255
24	24.054073250501247	25.826903541517638	25.794084597346668	24.324938610634447
25	24.133591056025644	25.86785756946613	25.623207413220527	24.3753439612877
26	24.076519632831666	25.9680822697034	25.870597648038622	24.084800449426307
27	24.122122890680757	25.716481295450944	25.81952827553134	24.341867538336963
28	24.19647963145403	25.80270849636978	25.63253310439353	24.368278767782662
29	24.08855864638321	25.938985491465015	25.810519520785785	24.161936341365987
30	24.130069522757672	25.72409103298844	25.756857269400886	24.388982174853002
31	24.220469354376135	25.83632367567566	25.57520854575383	24.367998424194376
32	24.19324025128343	25.946386319347255	25.691033804336826	24.16933962503249
33	24.13965244901064	25.77499938472691	25.669752727337812	24.415595438924637
34	24.258103246710412	25.882337682396226	25.51350479065343	24.346054280239933
35	24.251727025823712	25.940049361325144	25.65744275192796	24.15078086092318
36	24.208508833839222	25.802384361302423	25.608370086355936	24.380736718502423
37	24.27191779152462	25.884829759818345	25.498614647981476	24.344637800675557
38	24.23271707166041	25.98746299261048	25.66924945985056	24.11057047587855
39	24.209354802736254	25.73943034787479	25.64666574225047	24.404549107138486
40	24.302247995592424	25.802788097248246	25.514964783188965	24.379999123970368
41	24.241516611931743	25.898258483912983	25.66726668263816	24.192958221517106
42	24.22349557484647	25.697798941367843	25.660329139277415	24.418376344508268
43	24.319014101960743	25.76072258563905	25.48075502640783	24.439508285992375
44	24.273853822979717	25.848242264400756	25.655923424470405	24.22198048814912
45	24.25480612762211	25.6813441964792	25.606221781606763	24.457627894291925
46	24.327818941699757	25.74989008395292	25.46894624348011	24.45334473086721
47	24.29644770637601	25.836737871388987	25.630411676387986	24.23640274584701
48	24.30465373280814	25.63042903714169	25.59673686342287	24.4681803666273
49	24.401608343422453	25.711261517101335	25.43512317878002	24.45200696069619
50	24.361915154554477	25.80317338691885	25.59469836785037	24.240213090676303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2956.0
1	1884.5
2	813.0
3	824.5
4	836.0
5	843.5
6	851.0
7	1069.5
8	1288.0
9	1877.0
10	2466.0
11	4024.0
12	5582.0
13	8634.0
14	11686.0
15	18107.5
16	24529.0
17	35850.0
18	47171.0
19	64546.5
20	81922.0
21	108610.5
22	135299.0
23	170926.0
24	206553.0
25	252141.5
26	297730.0
27	349420.5
28	401111.0
29	457818.0
30	514525.0
31	585331.5
32	656138.0
33	739976.0
34	823814.0
35	917939.0
36	1012064.0
37	1109649.5
38	1207235.0
39	1311312.5
40	1415390.0
41	1508474.5
42	1601559.0
43	1687866.0
44	1774173.0
45	1861807.5
46	1949442.0
47	2018387.5
48	2087333.0
49	2137312.0
50	2187291.0
51	2189635.5
52	2191980.0
53	2147617.5
54	2103255.0
55	2010697.0
56	1918139.0
57	1798695.5
58	1679252.0
59	1534194.5
60	1389137.0
61	1216298.5
62	1043460.0
63	883371.0
64	723282.0
65	596412.0
66	469542.0
67	381391.0
68	293240.0
69	231804.5
70	170369.0
71	132391.5
72	94414.0
73	74531.0
74	54648.0
75	42741.0
76	30834.0
77	24331.5
78	17829.0
79	14227.5
80	10626.0
81	8040.0
82	5454.0
83	4152.5
84	2851.0
85	2178.0
86	1505.0
87	1115.0
88	725.0
89	556.0
90	387.0
91	273.5
92	160.0
93	106.0
94	52.0
95	48.0
96	44.0
97	44.5
98	45.0
99	129.5
100	214.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5053714851255275
2	0.0
3	0.0
4	0.0015775952987101658
5	9.074663222669095E-5
6	7.085217823853178E-4
7	0.0
8	6.980510171283919E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.980510171283919E-5
20	0.0028236163642843455
21	1.4310045851132034E-4
22	1.9196402971030776E-4
23	1.7451275428209797E-5
24	2.3035683565236933E-4
25	0.011709805812328774
26	0.023325374737345216
27	0.02776497920628179
28	0.04777461161226715
29	0.05988928701453038
30	0.08436644593013745
31	0.13449697972521293
32	0.15795498415581252
33	0.16061106827598606
34	0.16281341923502615
35	0.16695984227676877
36	0.19324495332673838
37	0.19273188582914902
38	0.21278340129616208
39	0.22263988165801496
40	0.23626583751236116
41	0.26279875667341135
42	0.2752380257986393
43	0.2955443298869042
44	0.29156543908927235
45	0.3181367510562646
46	0.332677153743049
47	0.33482715087580445
48	0.33369630822805646
49	0.3310925779341676
50	0.335835834595555
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.8651201E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.91466088377537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.84284182538492	45.08241786546533
2	11.848022471233408	13.72348407129666
3	4.181622978641414	7.265318302554603
4	1.904261463927459	4.4113862766960175
5	1.0932566041456802	3.165779274402246
6	0.7021824849385045	2.4399996296244115
7	0.47804146289021743	1.937992644816958
8	0.3235021704360833	1.4988414796776852
9	0.24773428026104313	1.291270214754403
>10	1.262948462247477	13.021258338200859
>50	0.08432322117650436	3.3421614589744997
>100	0.03110875027004057	2.727187511699316
>500	1.235897808804876E-4	0.04352397180993478
>1k	3.0234666417624096E-5	0.04937896002706572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.396102034256784E-5	5.933433645591331E-5	0.0	0.0	6.98051017128392E-6
2	2.094153051385176E-5	1.2564918308311055E-4	0.0	0.0	6.98051017128392E-6
3	2.792204068513568E-5	1.6753224411081408E-4	0.0	1.047076525692588E-5	1.047076525692588E-5
4	3.8392805942061556E-5	2.338470907380113E-4	0.0	1.047076525692588E-5	1.396102034256784E-5
5	5.584408137027136E-5	2.7922040685135675E-4	0.0	1.396102034256784E-5	2.094153051385176E-5
6	5.584408137027136E-5	3.176132127934183E-4	0.0	1.396102034256784E-5	2.792204068513568E-5
7	6.980510171283919E-5	3.5949627382112187E-4	0.0	1.7451275428209797E-5	3.141229577077764E-5
8	7.678561188412311E-5	4.048695899344673E-4	0.0	1.7451275428209797E-5	3.141229577077764E-5
9	9.423688731233291E-5	4.2232086536267714E-4	0.0	2.094153051385176E-5	3.8392805942061556E-5
10	3.42044998392912E-4	4.572234162190967E-4	0.0	2.094153051385176E-5	4.188306102770352E-5
11	4.886357119898744E-4	4.956162221611582E-4	0.0	2.443178559949372E-5	4.5373316113345475E-5
12	6.771094866145402E-4	5.409895382745037E-4	0.0	2.792204068513568E-5	4.886357119898744E-5
13	8.58602751067922E-4	5.863628543878492E-4	0.0	2.792204068513568E-5	5.2353826284629394E-5
14	0.0010470765256925879	6.282459154155528E-4	0.0	3.141229577077764E-5	6.631484662719724E-5
15	0.0013542189732290804	6.491874459294045E-4	0.0	3.141229577077764E-5	6.980510171283919E-5
16	0.0015706147885388819	6.561679561006885E-4	0.0	3.141229577077764E-5	8.725637714104899E-5
17	0.0018463449403045968	6.9456076204275E-4	0.0	3.8392805942061556E-5	9.423688731233291E-5
18	0.0021918801937831508	7.294633128991695E-4	0.0	4.188306102770352E-5	9.423688731233291E-5
19	0.00261769131423147	7.78326884098157E-4	0.0	4.5373316113345475E-5	1.0470765256925879E-4
20	0.005800803952336937	8.481319858109962E-4	0.0	4.5373316113345475E-5	1.1517841782618466E-4
21	0.006226615072785256	8.969955570099836E-4	0.0	5.2353826284629394E-5	1.1517841782618466E-4
22	0.006579130836435094	9.039760671812675E-4	0.0	5.933433645591331E-5	1.2564918308311055E-4
23	0.006963058895855709	9.423688731233291E-4	0.0	5.933433645591331E-5	1.3262969325439448E-4
24	0.007395850526475313	9.737811688941068E-4	0.0	6.282459154155528E-5	1.5008096868260426E-4
25	0.007779778585895928	9.842519341510326E-4	0.0	6.282459154155528E-5	1.570614788538882E-4
26	0.008188138430916037	0.00103311550535002	0.0	6.631484662719724E-5	1.6404198902517211E-4
27	0.008579047000507937	0.0010924498418059334	0.0	6.631484662719724E-5	1.71022499196456E-4
28	0.010854693316346494	0.001113391372319785	0.0	6.631484662719724E-5	1.71022499196456E-4
29	0.01130842647747995	0.0011482939231762046	0.0	6.980510171283919E-5	1.71022499196456E-4
30	0.011912240607296008	0.0011831964740326244	0.0	6.980510171283919E-5	1.7451275428209798E-4
31	0.015622381763333412	0.00121111851471776	0.0	6.980510171283919E-5	1.814932644533819E-4
32	0.016177332321950483	0.0012530015757454636	0.0	6.980510171283919E-5	2.059250500528756E-4
33	0.016952168950963	0.0013018651469444509	0.0	7.329535679848115E-5	2.1988607039544346E-4
34	0.017493158489237503	0.0013332774427152285	0.0	7.329535679848115E-5	2.1988607039544346E-4
35	0.01809348236396792	0.0013472384630577965	0.0	7.329535679848115E-5	2.443178559949372E-4
36	0.018746160064982965	0.001382141013914216	0.0	7.678561188412311E-5	2.6525938650878895E-4
37	0.019388367000741084	0.0014030825444280677	0.0	8.725637714104899E-5	2.6525938650878895E-4
38	0.02010735954838333	0.0014554363707126971	0.0	9.074663222669095E-5	2.687496415944309E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	9045	0.0	13.2625885	9
CAACGCA	9395	0.0	12.74512	10
ATCAACG	10055	0.0	12.039647	8
AACGCAG	10600	0.0	11.482808	11
GTATCAA	10970	0.0	11.195769	6
ACGCAGA	11430	0.0	10.687418	12
GGTATCA	11805	0.0	10.329433	5
AGTGGTA	11935	0.0	10.290737	2
AGAGTAC	12225	0.0	9.920849	16
TATCAAC	12520	0.0	9.914901	7
CGCAGAG	12740	0.0	9.760948	13
GTGGTAT	12525	0.0	9.735816	3
TGGTATC	13485	0.0	9.221947	4
GAGTACA	12165	0.0	7.6579475	17
CAGAGTA	16640	0.0	7.592257	15
CAGTGGT	18040	0.0	7.393622	1
GTACATG	12595	0.0	7.240283	19
AGTACAT	12930	0.0	7.170894	18
GCAGAGT	19245	0.0	6.895653	14
ACATGGG	13480	0.0	6.7335463	21
>>END_MODULE