FastQCFastQC Report
Fri 27 May 2016
SRR522032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28026279
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC73800.013.1589899
CAACGCA78650.012.31959810
ATCAACG88050.011.07924658
AACGCAG90150.010.82116111
GTATCAA93600.010.65726956
GGTATCA98250.010.2423325
ACGCAGA98750.010.05672112
AGTGGTA101500.09.8061062
TATCAAC108200.09.4017867
GTGGTAT107050.09.4003323
AGAGTAC109550.09.04536716
CGCAGAG112500.08.94474713
TGGTATC119750.08.458434
GAGTACA115700.07.349193617
GTACATG124050.07.245255519
CAGAGTA146050.07.05552715
AGTACAT124000.06.94610218
CAGTGGT163450.06.6606061
GCAGAGT171700.06.359789414
TACATGG146450.06.137705320
Produced by FastQC (version 0.11.5)