##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522032_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28026279
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.12835913750805	40.0	39.0	40.0	38.0	40.0
2	39.12451010710341	40.0	39.0	40.0	38.0	40.0
3	39.11351902976489	40.0	39.0	40.0	38.0	40.0
4	39.10548053846178	40.0	39.0	40.0	38.0	40.0
5	39.10009191730376	40.0	39.0	40.0	38.0	40.0
6	39.15658421868989	40.0	39.0	40.0	38.0	40.0
7	39.13457369777843	40.0	39.0	40.0	38.0	40.0
8	39.11654658115692	40.0	39.0	40.0	38.0	40.0
9	39.0937710996169	40.0	39.0	40.0	38.0	40.0
10	39.07405132161854	40.0	39.0	40.0	38.0	40.0
11	39.1064308965168	40.0	39.0	40.0	38.0	40.0
12	39.07049562305435	40.0	39.0	40.0	38.0	40.0
13	39.04046655640587	40.0	39.0	40.0	38.0	40.0
14	39.00144853335686	40.0	39.0	40.0	38.0	40.0
15	38.98016293921858	40.0	39.0	40.0	38.0	40.0
16	38.93234414029775	40.0	39.0	40.0	38.0	40.0
17	38.88054932301216	40.0	39.0	40.0	37.0	40.0
18	38.82647050648429	40.0	39.0	40.0	37.0	40.0
19	38.781929880880725	40.0	39.0	40.0	36.0	40.0
20	38.73137311592452	40.0	39.0	40.0	36.0	40.0
21	38.634092131888075	40.0	39.0	40.0	36.0	40.0
22	38.55405307283211	40.0	39.0	40.0	36.0	40.0
23	38.48228935421645	40.0	38.0	40.0	35.0	40.0
24	38.65665006760263	40.0	39.0	40.0	36.0	40.0
25	38.618924866907946	40.0	39.0	40.0	36.0	40.0
26	38.577379002043045	40.0	39.0	40.0	36.0	40.0
27	38.50925226284945	40.0	39.0	40.0	36.0	40.0
28	38.44870055707359	40.0	39.0	40.0	36.0	40.0
29	38.52468146056778	40.0	39.0	40.0	36.0	40.0
30	38.47866393537294	40.0	39.0	40.0	36.0	40.0
31	38.4318463753251	40.0	39.0	40.0	36.0	40.0
32	38.3621501091886	40.0	39.0	40.0	36.0	40.0
33	38.30111103939271	40.0	39.0	40.0	36.0	40.0
34	38.25839402369469	40.0	39.0	40.0	35.0	40.0
35	38.19506299783856	40.0	39.0	40.0	35.0	40.0
36	38.06337080994591	40.0	39.0	40.0	35.0	40.0
37	37.9846365976732	40.0	39.0	40.0	35.0	40.0
38	37.91698109477894	40.0	38.0	40.0	35.0	40.0
39	37.854740973641206	40.0	38.0	40.0	35.0	40.0
40	37.76626361994041	40.0	38.0	40.0	35.0	40.0
41	37.58803032682291	40.0	38.0	40.0	34.0	40.0
42	37.4942701098494	40.0	38.0	40.0	34.0	40.0
43	37.41608984196582	40.0	38.0	40.0	33.0	40.0
44	37.33716652146366	40.0	38.0	40.0	33.0	40.0
45	37.21891917938874	40.0	38.0	40.0	33.0	40.0
46	37.03558742136264	40.0	38.0	40.0	33.0	40.0
47	36.934093926632215	39.0	38.0	40.0	33.0	40.0
48	36.83250280923843	39.0	38.0	40.0	33.0	40.0
49	36.744722515607585	39.0	37.0	40.0	32.0	40.0
50	36.6349598888957	39.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1984816377832317
1	2	-0.1932614866446798
1	3	-0.18789538748917067
1	4	-0.18420300010746615
1	5	-0.19641709264411844
1	6	-0.16477305809366527
1	7	-0.17309711843280695
1	8	-0.16762462550506996
1	9	-0.18346857184146614
1	10	-0.18438190003732302
1	11	-0.16949192622501386
1	12	-0.1748410199359114
1	13	-0.15929684835655422
1	14	-0.16567419149638596
1	15	-0.1771555530812492
1	16	-0.1786348562352913
1	17	-0.1854840030861027
1	18	-0.16854407113640946
1	19	-0.20769588771128156
1	20	-0.18862108230943164
1	21	-0.18847294559145666
1	22	-0.2050604128216733
1	23	-0.1732133856706426
1	24	-0.21988850020564144
1	25	-0.24306582557154144
1	26	-0.25368551944267637
1	27	-0.29850640037012255
1	28	-0.26510661630136667
1	29	-0.20631905351324065
1	30	-0.16302802992264986
1	31	-0.1270144888998317
1	32	-0.13543606014442133
1	33	-0.2168505281353461
1	34	-0.1571448184469233
1	35	-0.1292934381684816
1	36	-0.08030423380783702
1	37	-0.077860530209513
1	38	-0.09565215261101656
1	39	-0.10949813493497373
1	40	-0.09632429156451394
1	41	-0.08286402735743081
1	42	-0.1055074362306172
1	43	-0.13074733325197485
1	44	-0.054219057239379254
1	45	-0.08236568494746166
1	46	-0.1197566263913501
1	47	-0.10381184751051364
1	48	-0.14064587039603538
1	49	-0.14150090134297244
1	50	-0.07594892087966088
2	1	-0.15288211783051509
2	2	-0.15893404625062857
2	3	-0.1663115893474867
2	4	-0.15344409787198998
2	5	-0.1412612101863715
2	6	-0.14847171532547065
2	7	-0.15542573159701334
2	8	-0.14376391109158249
2	9	-0.1802934157602536
2	10	-0.16133594825982556
2	11	-0.14180786727720118
2	12	-0.16287315866401286
2	13	-0.15742094120145822
2	14	-0.15921479900919877
2	15	-0.17362056587670338
2	16	-0.19466017918205125
2	17	-0.16884047711817374
2	18	-0.203337196039989
2	19	-0.18789112619695203
2	20	-0.1808597648982868
2	21	-0.2189734388036939
2	22	-0.2199684814256031
2	23	-0.2055116727403714
2	24	-0.21836744788710405
2	25	-0.25012503510363615
2	26	-0.29062287597273695
2	27	-0.3227249782614763
2	28	-0.31576810247381815
2	29	-0.21503474431774805
2	30	-0.2400416127114937
2	31	-0.1629960939421835
2	32	-0.1762393900622783
2	33	-0.23956891075013687
2	34	-0.19360852929963812
2	35	-0.19031578357016343
2	36	-0.19398959593393528
2	37	-0.20186984744591996
2	38	-0.23704708922036843
2	39	-0.27727203209018114
2	40	-0.2577277174725836
2	41	-0.243113885839918
2	42	-0.2942759546182927
2	43	-0.22404772941786177
2	44	-0.24283847116591062
2	45	-0.24012924740495833
2	46	-0.22073825308522999
2	47	-0.25934503556537436
2	48	-0.23699527058077052
2	49	-0.25907406064389704
2	50	-0.26718399052691666
3	1	-0.12414848737204665
3	2	-0.11777969734668403
3	3	-0.11432951975209704
3	4	-0.13204200961862966
3	5	-0.12930781048021345
3	6	-0.13767752706760206
3	7	-0.1236215956114961
3	8	-0.12310854210050337
3	9	-0.12933401481843276
3	10	-0.1340059813733845
3	11	-0.10455641591242681
3	12	-0.14044215967066975
3	13	-0.14016865209497098
3	14	-0.11774755812521676
3	15	-0.14027874585549682
3	16	-0.11718541046023034
3	17	-0.13461634660529853
3	18	-0.14385104131546456
3	19	-0.11886653073742082
3	20	-0.12252929309034499
3	21	-0.1404419863826476
3	22	-0.11811091208767976
3	23	-0.1497111262960189
3	24	-0.21406733328949912
3	25	-0.19786039158322666
3	26	-0.21635092588757487
3	27	-0.18941242001224623
3	28	-0.2510461257311718
3	29	-0.19195083037021732
3	30	-0.1464096107956152
3	31	-0.18411300853942691
3	32	-0.19965232251798426
3	33	-0.17670490400449523
3	34	-0.18015315885161698
3	35	-0.13883882670339176
3	36	-0.166405952658927
3	37	-0.13567917743010582
3	38	-0.12940844638016813
3	39	-0.15764868805287335
3	40	-0.14787635288693224
3	41	-0.13599301746598513
3	42	-0.16211347005846477
3	43	-0.15463793211948484
3	44	-0.14986739283295947
3	45	-0.19038574661889385
3	46	-0.12727497872618443
3	47	-0.11328199823260121
3	48	-0.16011145124341652
3	49	-0.1787351349292834
3	50	-0.2116132994673947
4	1	-0.13820179311812808
4	2	-0.12887750194086323
4	3	-0.1441070565176048
4	4	-0.1420804998120886
4	5	-0.15315504649946377
4	6	-0.12320676886459836
4	7	-0.14480088898052657
4	8	-0.14820275392740712
4	9	-0.15074176347586388
4	10	-0.15123571276090075
4	11	-0.1379787715156624
4	12	-0.1611117082391047
4	13	-0.15089396254687415
4	14	-0.1365993416269191
4	15	-0.13996714056395376
4	16	-0.12479934855461039
4	17	-0.15865340559260943
4	18	-0.14793456308246533
4	19	-0.13915920349582223
4	20	-0.1478620839534912
4	21	-0.1536915503631775
4	22	-0.12995909269220363
4	23	-0.13016876946178968
4	24	-0.1676946859275148
4	25	-0.178691297686008
4	26	-0.19065719745383092
4	27	-0.20813216494494924
4	28	-0.20250655416013785
4	29	-0.12996818964736434
4	30	-0.16186949505965487
4	31	-0.07336611405992954
4	32	-0.11261609129456929
4	33	-0.151114204849236
4	34	-0.12151529903132285
4	35	-0.0998855584456777
4	36	-0.09706221178620211
4	37	-0.09563671038014121
4	38	-0.1107058213814085
4	39	-0.14823973751815345
4	40	-0.09612184660779377
4	41	-0.10336887724213994
4	42	-0.12206875671046902
4	43	-0.1101119497271199
4	44	-0.11615682702430519
4	45	-0.13625735011863327
4	46	-0.11122838082966524
4	47	-0.10292682469663816
4	48	-0.11131436733266753
4	49	-0.10250287772753808
4	50	-0.10705579572077539
5	1	-0.0852653038534612
5	2	-0.10865541058397099
5	3	-0.10403892678287008
5	4	-0.10479682960701808
5	5	-0.08623950624543397
5	6	-0.09543342007253841
5	7	-0.09582028744690518
5	8	-0.08916797373435514
5	9	-0.10663919297027746
5	10	-0.09514278937960796
5	11	-0.09186724174343652
5	12	-0.108265550921395
5	13	-0.08802628329398487
5	14	-0.10241895719082805
5	15	-0.09775998371210903
5	16	-0.1051822529520976
5	17	-0.10028992033065265
5	18	-0.1091880768745952
5	19	-0.08067353309466085
5	20	-0.09303663887855151
5	21	-0.08731585592446578
5	22	-0.09177480637320201
5	23	-0.09694441214619332
5	24	-0.14903219880662988
5	25	-0.13765095806990502
5	26	-0.17293144178465525
5	27	-0.2107811950723999
5	28	-0.1776965644471673
5	29	-0.15150962605584795
5	30	-0.12504022843320683
5	31	-0.07779675372761119
5	32	-0.05188837194110363
5	33	-0.11091959979957267
5	34	-0.12519268661770866
5	35	-0.05061547360607932
5	36	-0.02660847250741938
5	37	-0.0806932247716361
5	38	-0.054719822260125284
5	39	-0.04789667560116584
5	40	-0.06010787999441902
5	41	-0.04313089195986919
5	42	-0.10718568861124567
5	43	-0.034848398664763636
5	44	-0.10473702547574959
5	45	-0.05462442196751027
5	46	-0.01969092251193416
5	47	-0.04095882566090836
5	48	-0.018222459218740994
5	49	-0.024466507448984487
5	50	-0.09066255929072753
6	1	-0.11321421310317703
6	2	-0.10986722410011396
6	3	-0.10611296462811737
6	4	-0.12573689404943877
6	5	-0.12578622978315934
6	6	-0.1155128271948982
6	7	-0.11847288530624667
6	8	-0.12620656963075305
6	9	-0.13672464282215202
6	10	-0.1262403164625212
6	11	-0.10786927561743909
6	12	-0.14588166430721827
6	13	-0.12685058894335555
6	14	-0.1255116991279266
6	15	-0.14446293892378037
6	16	-0.1245623543907115
6	17	-0.11384776430443821
6	18	-0.11272815619967247
6	19	-0.127369728320474
6	20	-0.1341719669504826
6	21	-0.1321606496119685
6	22	-0.1531020125806748
6	23	-0.11485225593570192
6	24	-0.19041932652832827
6	25	-0.1592849969075374
6	26	-0.16751643378147207
6	27	-0.14514833839141517
6	28	-0.16314353456248654
6	29	-0.13286440942207633
6	30	-0.11217536395320593
6	31	-0.11998382777806427
6	32	-0.131854573897904
6	33	-0.12005418589193084
6	34	-0.13914748054435222
6	35	-0.1614655817489563
6	36	-0.17131905587824292
6	37	-0.1467942525165995
6	38	-0.17657869860446596
6	39	-0.17368717606385786
6	40	-0.29994955801371503
6	41	-0.20185910364804727
6	42	-0.23032341250954858
6	43	-0.21224164485735741
6	44	-0.2463566544314304
6	45	-0.2869388879592307
6	46	-0.2005114233784795
6	47	-0.23807012543306172
6	48	-0.269726071587165
6	49	-0.24227551178943685
6	50	-0.24961068977467704
7	1	-0.0730831372891032
7	2	-0.060288188739001214
7	3	-0.07642677203448756
7	4	-0.05939157597001099
7	5	-0.06277123703483056
7	6	-0.07034141118307957
7	7	-0.07227536101854781
7	8	-0.08460787240550616
7	9	-0.0859796295811961
7	10	-0.08846157706291535
7	11	-0.0905465316645433
7	12	-0.102962417783246
7	13	-0.07514636917568396
7	14	-0.079247504815676
7	15	-0.11693880510418353
7	16	-0.08872077551704649
7	17	-0.0900475384634234
7	18	-0.11573663034452153
7	19	-0.09094814594507028
7	20	-0.11902891188715614
7	21	-0.07288927889199215
7	22	-0.10344638747749713
7	23	-0.09875951727445909
7	24	-0.13865361244789653
7	25	-0.13532234957999378
7	26	-0.1208144577348591
7	27	-0.1287001897428155
7	28	-0.13559856298751782
7	29	-0.11413075944765438
7	30	-0.1041522059679636
7	31	-0.09464020340707435
7	32	-0.08836028849241018
7	33	-0.11987375019841551
7	34	-0.09876073631205173
7	35	-0.1080945954957997
7	36	-0.09127619825459021
7	37	-0.11074802749008938
7	38	-0.08561060535994613
7	39	-0.11166432965297446
7	40	-0.19817118994485128
7	41	-0.20192891782626532
7	42	-0.23345963833145333
7	43	-0.20003180105822338
7	44	-0.24220544311448577
7	45	-0.23971952484208003
7	46	-0.24005177763444863
7	47	-0.23420416392551147
7	48	-0.18914929032710148
7	49	-0.19415471299315357
7	50	-0.20278228891241667
8	1	-0.09033062414971482
8	2	-0.10359153132228727
8	3	-0.08994952244405852
8	4	-0.0884106531584905
8	5	-0.09980162623458
8	6	-0.10184602237753637
8	7	-0.0978677795351004
8	8	-0.10733561496280686
8	9	-0.11282662888176986
8	10	-0.08688388521903079
8	11	-0.09439235937120571
8	12	-0.09042561707347829
8	13	-0.12901977481705984
8	14	-0.09553646226647317
8	15	-0.10970580170935307
8	16	-0.09379986367518
8	17	-0.08982693955782395
8	18	-0.11230289840255381
8	19	-0.10941522220422684
8	20	-0.09801911232706573
8	21	-0.06892116642509905
8	22	-0.07393074535168154
8	23	-0.08243901651770358
8	24	-0.14563653354356632
8	25	-0.11386249825530825
8	26	-0.16996611337372514
8	27	-0.14544511063550658
8	28	-0.158022879333231
8	29	-0.12578762175042613
8	30	-0.10772076404224862
8	31	-0.08140794372518201
8	32	-0.10218961800813275
8	33	-0.10072513812398398
8	34	-0.11247751100226822
8	35	-0.09639675354171118
8	36	-0.1101351030382034
8	37	-0.1393319414054517
8	38	-0.07761368401468616
8	39	-0.13537231287327245
8	40	-0.11952222875117258
8	41	-0.06931257207586583
8	42	-0.09891136601268613
8	43	-0.08331930328224502
8	44	-0.0556639832945649
8	45	-0.16892810024809535
8	46	-0.03306472203453836
8	47	-0.02331383124209907
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113	4	-0.05401473183883354
113	5	-0.04338157648491858
113	6	-0.04237324198503245
113	7	-0.037187537094929723
113	8	-0.04304261711084223
113	9	-0.030787796966158965
113	10	-0.030238243780566165
113	11	-0.04120722767754614
113	12	-0.01729402845705863
113	13	-0.022765882894645983
113	14	-0.001280248994717681
113	15	-0.030383241223255197
113	16	-0.004819414025391211
113	17	0.011977212972510642
113	18	0.010987384418498891
113	19	0.01900276591375416
113	20	0.04061279179789068
113	21	0.080175933038376
113	22	0.042053725009616016
113	23	0.07139343880491111
113	24	0.015515015114964115
113	25	0.013041133067865474
113	26	0.020966927516596456
113	27	0.01304604594982095
113	28	0.05570243356896043
113	29	0.045732408155231496
113	30	0.09183172694552155
113	31	0.08617615864189077
113	32	0.12985953274768747
113	33	0.1727156300346877
113	34	0.17114487613439167
113	35	0.16221294161712052
113	36	0.2137683134620616
113	37	0.21291401218608286
113	38	0.24090427975678352
113	39	0.2803911667518051
113	40	0.2825812595745134
113	41	0.3938856760433609
113	42	0.41573453083345413
113	43	0.4206786627470649
113	44	0.40296488886566806
113	45	0.45866304697634774
113	46	0.4928809646905705
113	47	0.4697749119698642
113	48	0.5798086687811548
113	49	0.542335549036224
113	50	0.5983961750576583
114	1	-0.102154220576395
114	2	-0.1013760174144025
114	3	-0.09256739998519947
114	4	-0.09923994678959502
114	5	-0.09207844797520437
114	6	-0.0967699793685668
114	7	-0.09045245304425009
114	8	-0.09116404580746718
114	9	-0.07753119010715892
114	10	-0.08820867897992457
114	11	-0.07315341261840302
114	12	-0.07855827742750421
114	13	-0.054172554098755654
114	14	-0.07307840370596352
114	15	-0.06183540350296113
114	16	-0.03606077324177903
114	17	-0.04032107825312892
114	18	-0.04730439569072331
114	19	-0.04077617234073472
114	20	-0.028583763242274074
114	21	0.00807342557511248
114	22	0.035271114327628084
114	23	0.031111181187775117
114	24	-0.02170717195603089
114	25	-0.016211146608640092
114	26	-0.03604358251029538
114	27	-0.05200680746845876
114	28	-0.009950341830020193
114	29	-0.00500534125986718
114	30	0.02495195306895681
114	31	0.031181167054214143
114	32	0.023202382427982116
114	33	0.0638371226173291
114	34	0.09879683273892681
114	35	0.12224219890911314
114	36	0.14193629869497926
114	37	0.1311826851464133
114	38	0.11410517647142626
114	39	0.14643690384091457
114	40	0.14666518691712582
114	41	0.19838717831404296
114	42	0.26640931614453933
114	43	0.27721964696569756
114	44	0.266758284742707
114	45	0.31834947573167227
114	46	0.40676804581929815
114	47	0.4169595501297749
114	48	0.44436456940160696
114	49	0.4308870009510599
114	50	0.41355485032023154
115	1	-0.057540427304310526
115	2	-0.024056711078799253
115	3	-0.025647401289582206
115	4	-0.06011517517202947
115	5	-0.02780445972717871
115	6	-0.02237574920483354
115	7	-0.031757427229010204
115	8	-0.04081010469339219
115	9	-0.026669230821930512
115	10	-0.020216487195476418
115	11	-8.909025901999712E-4
115	12	-0.008875393234227147
115	13	-0.006131617890076768
115	14	-0.01319797295533931
115	15	-0.0052963088217339305
115	16	-0.022060471213841026
115	17	0.015676450321407742
115	18	0.005091419813048503
115	19	0.03237440394257618
115	20	-0.012130957599083558
115	21	0.07234142232488949
115	22	0.07763422021560729
115	23	0.05979451164299121
115	24	8.920496567341729E-4
115	25	0.0214015831895793
115	26	0.01841733538987711
115	27	0.0046892139808463185
115	28	0.04052349309890957
115	29	0.08325668898519467
115	30	0.06758846579650424
115	31	0.12342777972949648
115	32	0.13612726516429774
115	33	0.1341815959168713
115	34	0.14542357341746737
115	35	0.17310531788706385
115	36	0.16428768313355135
115	37	0.21208096804196686
115	38	0.19142533809897344
115	39	0.2422731738312578
115	40	0.242312881367738
115	41	0.31830112222426976
115	42	0.37814390274468224
115	43	0.29006994809609665
115	44	0.36893336238870234
115	45	0.4098386828449634
115	46	0.48028513548853624
115	47	0.5166379875325404
115	48	0.5321728939987693
115	49	0.5420906947752115
115	50	0.5694787930506067
116	1	-0.04826623231380722
116	2	-0.02740999379269482
116	3	-0.02922772124244233
116	4	-0.03401826079960557
116	5	-0.015291582851723717
116	6	-0.02856300869086681
116	7	-0.022181223650527215
116	8	-0.033174663421810635
116	9	-0.03702188361776848
116	10	-0.025416586127974483
116	11	-0.05288419276783429
116	12	-0.027424061497669072
116	13	-0.029453365844709367
116	14	0.01952299094987353
116	15	-0.012076567560697526
116	16	-0.025287290021708486
116	17	-0.005670729163988142
116	18	0.02812146593650766
116	19	-0.0019019629392573734
116	20	-0.0010340951211773586
116	21	0.04567966675912771
116	22	0.05497773218588975
116	23	0.04763699112875486
116	24	0.011633153809903263
116	25	0.003149058969498242
116	26	0.0040400383551997265
116	27	0.03374401295575069
116	28	-0.02232422402300216
116	29	0.07840183548548652
116	30	0.0787285238921882
116	31	0.15236912192546015
116	32	0.1593045536155131
116	33	0.16130801099979664
116	34	0.18265805165042792
116	35	0.23649703594004023
116	36	0.2260373389986725
116	37	0.24073654509867737
116	38	0.26032944981572825
116	39	0.2831319818686495
116	40	0.30596383496794743
116	41	0.34452499522704727
116	42	0.4104010522867867
116	43	0.3995853098584661
116	44	0.39048520314403135
116	45	0.42828860207142583
116	46	0.5258783007317902
116	47	0.5842908692795845
116	48	0.5937154252790862
116	49	0.5845242618784994
116	50	0.5735439737639751
117	1	-0.0659523120471519
117	2	-0.06741967123792136
117	3	-0.050013730419301794
117	4	-0.08048876572075159
117	5	-0.06685185563427609
117	6	-0.07703396745980484
117	7	-0.06786742844848703
117	8	-0.07784123734850112
117	9	-0.06976939585156572
117	10	-0.08287865118185067
117	11	-0.06556476652296794
117	12	-0.056931954186843825
117	13	-0.07139380984285282
117	14	-0.030949785490072657
117	15	-0.044480070656639725
117	16	-0.027061023527636507
117	17	-0.026597110353556275
117	18	-0.028373648462000745
117	19	-0.011059323344518646
117	20	-0.029862732607647047
117	21	0.02037163710952683
117	22	0.014778951492687042
117	23	0.013469525200619614
117	24	-0.007079009510491119
117	25	-0.003534010622814776
117	26	-3.546682031796422E-4
117	27	-0.05241793042474541
117	28	-0.015660858622723595
117	29	0.050861023686614715
117	30	0.052339939149440795
117	31	0.10953925146138488
117	32	0.11143052194525893
117	33	0.14123044691343978
117	34	0.12511699680604949
117	35	0.1831184204192695
117	36	0.1600407838125406
117	37	0.19025773323214423
117	38	0.228554699752614
117	39	0.24653954667437006
117	40	0.27213472950691653
117	41	0.3284774167684361
117	42	0.36700022843172064
117	43	0.3617419129143826
117	44	0.3713803401616502
117	45	0.39501570239072237
117	46	0.49872986290578325
117	47	0.5197519052372499
117	48	0.5259989363592936
117	49	0.5450980259666451
117	50	0.5952838042533699
118	1	-0.07745201867841445
118	2	-0.08776132722442043
118	3	-0.07578091136409881
118	4	-0.05682340634393057
118	5	-0.0662329157927104
118	6	-0.07007113245931862
118	7	-0.07222578645423283
118	8	-0.0742817735926593
118	9	-0.06712094913536504
118	10	-0.11425675891698717
118	11	-0.0828490803443529
118	12	-0.06738410229486647
118	13	-0.07006629162572153
118	14	-0.05725260489290207
118	15	-0.05623978414212871
118	16	-0.03890506014736417
118	17	-0.08586284172110226
118	18	-0.045870342926257024
118	19	-0.04642979568586725
118	20	-0.031124623432411624
118	21	-0.05541801316655892
118	22	-0.005008301411827176
118	23	-0.04678036448474643
118	24	-0.06872502969505234
118	25	-0.06761579839174203
118	26	-0.04444895140923677
118	27	-0.08181311852537476
118	28	-0.04897514376758494
118	29	-0.0126329531962881
118	30	0.013928547276542247
118	31	0.03789764501027548
118	32	0.05848138547614923
118	33	0.09309491005186743
118	34	0.08557412508774576
118	35	0.10714372994200261
118	36	0.14203311067137037
118	37	0.19011340807460897
118	38	0.2027848668481198
118	39	0.2244115317807811
118	40	0.22454669603996535
118	41	0.3202320492828221
118	42	0.30540070364452987
118	43	0.333123611242236
118	44	0.3724796233794194
118	45	0.3753155164229085
118	46	0.4460485514058661
118	47	0.4548979552018224
118	48	0.481237392539434
118	49	0.4609996711414155
118	50	0.5075943171405441
119	1	-0.06896541022105396
119	2	-0.07563444354882876
119	3	-0.09176840142963272
119	4	-0.09746516641056502
119	5	-0.09133453858451901
119	6	-0.08630755252388411
119	7	-0.08696422670503523
119	8	-0.07604984619748478
119	9	-0.10504354982245445
119	10	-0.09162900556262343
119	11	-0.06387413262226005
119	12	-0.04602391088275937
119	13	-0.06374822440387362
119	14	-0.0418060998612404
119	15	-0.047805508590450074
119	16	-0.08837718442131148
119	17	-0.05432234377447287
119	18	-0.041126892324200526
119	19	-0.04604905820488625
119	20	-0.031225323169287833
119	21	-0.021191530253290125
119	22	-0.034706621544827954
119	23	6.867574664823906E-4
119	24	-0.06981857196871033
119	25	-0.059156416653024735
119	26	-0.07228499970719326
119	27	-0.10600192900398753
119	28	-0.055386344283988365
119	29	-0.02646876989136615
119	30	0.05597237562579238
119	31	0.0344029663992913
119	32	0.07924194838271603
119	33	0.09165205272012145
119	34	0.06887359209495969
119	35	0.08859903184593776
119	36	0.10565572265511491
119	37	0.1528360755164897
119	38	0.16244905866918202
119	39	0.14258896854431669
119	40	0.21966472482916544
119	41	0.24063955104779922
119	42	0.31328593444126085
119	43	0.2967903291494096
119	44	0.32872586978333374
119	45	0.409778801481977
119	46	0.4260888237029903
119	47	0.46599734492643563
119	48	0.4221116983800144
119	49	0.5252956097427983
119	50	0.5127687402771315
120	1	-0.09228142969853792
120	2	-0.09054944150686595
120	3	-0.09382390745560798
120	4	-0.09418782820493732
120	5	-0.10835321446444368
120	6	-0.07839954142698957
120	7	-0.0887579423941176
120	8	-0.08583884850155243
120	9	-0.07983861589033125
120	10	-0.10188746585109243
120	11	-0.09749334920531538
120	12	-0.09481368358613196
120	13	-0.07929967309473085
120	14	-0.06321626072406161
120	15	-0.07990459275609396
120	16	-0.07466071972641686
120	17	-0.06783372618529881
120	18	-0.08890851257983456
120	19	-0.09069448772667243
120	20	-0.036786885701665994
120	21	-0.009167506320622465
120	22	-0.024938700539493652
120	23	-0.010014493673907054
120	24	-0.11171050583812558
120	25	-0.0845540242390399
120	26	-0.12895415506477548
120	27	-0.1414614981124913
120	28	-0.12867918603386386
120	29	-0.06499630176941196
120	30	0.010465031062345531
120	31	0.05210838223146652
120	32	0.05912139912559411
120	33	0.08803302028849203
120	34	0.09215479274443084
120	35	0.09804323080157928
120	36	0.17536166792960017
120	37	0.13936514791455323
120	38	0.21180434898167988
120	39	0.22057357911266706
120	40	0.2600819846598341
120	41	0.31576887284985133
120	42	0.3217392956407963
120	43	0.34325130508009494
120	44	0.3793934019895673
120	45	0.40796393484536253
120	46	0.4889174236063738
120	47	0.537546868858783
120	48	0.5687718197310687
120	49	0.5648863974130549
120	50	0.5603818466545576
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12895.0
3	492.0
4	838.0
5	972.0
6	1729.0
7	3112.0
8	3665.0
9	4139.0
10	5605.0
11	8512.0
12	8219.0
13	10475.0
14	14731.0
15	17610.0
16	18125.0
17	20140.0
18	26038.0
19	34772.0
20	44642.0
21	34945.0
22	47153.0
23	64766.0
24	72290.0
25	66827.0
26	91197.0
27	120472.0
28	141616.0
29	136679.0
30	147986.0
31	181255.0
32	212792.0
33	268312.0
34	378319.0
35	563525.0
36	906050.0
37	1748049.0
38	3890351.0
39	1.8365239E7
40	351745.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.075325754334102	29.532301882537798	20.8740832567214	31.518289106406698
2	24.691640299448956	28.362887559921884	25.882911534563686	21.06256060606547
3	22.949168528579907	26.88425388186566	25.41160030555608	24.75497728399835
4	25.31367444495202	25.92180743560212	24.356468971558865	24.408049147886995
5	25.679056431287222	27.362512162246013	25.299737435711677	21.658693970755092
6	25.447332404647106	26.153042119397043	23.26172392028808	25.137901555667774
7	24.72856278923078	25.784878542028363	25.569687649223788	23.916871019517075
8	23.788709161141227	25.2933541409475	26.189423861797707	24.728512836113563
9	23.670982901902484	24.9030160125448	26.453596617688348	24.97240446786437
10	24.353650372209596	26.327961696235164	24.319714365221298	24.99867356633394
11	24.533571311996177	25.952077000384143	24.84411105927837	24.67024062834131
12	24.368732645528862	25.843073923584363	25.21597319430096	24.572220236585814
13	24.510581658021742	25.868514332566235	25.223719495549158	24.397184513862864
14	24.28936427843311	25.955764587942625	25.551554667674576	24.20331646594969
15	23.96472967388928	25.80802467569812	25.473274564918164	24.753971085494438
16	24.268344615900613	25.89266322406385	25.486251740554177	24.35274041948136
17	24.305666835044352	25.825212116100037	25.630744630780278	24.238376418075337
18	24.076785220042947	25.823806292658404	25.379384113031918	24.72002437426674
19	24.371675083057806	25.784860229675992	25.434549535392943	24.408915151873256
20	24.29609192042537	25.758805186614303	25.608595026908343	24.33650786605199
21	24.201268519219912	25.708860284802576	25.483478489661522	24.606392706315987
22	24.24789836718432	25.68832528974991	25.373033089981305	24.69074325308447
23	24.188690906844965	25.79910447619536	25.706230927052427	24.305973689907248
24	24.29206287810654	25.56555702188752	25.54842609374696	24.59395400625898
25	24.388540009045318	25.60972572218199	25.34288741762313	24.658846851149566
26	24.307762563726357	25.74093377340975	25.641235651443147	24.310068011420746
27	24.36841965887206	25.45853025774712	25.572532460631	24.60051762274982
28	24.453189184465433	25.549905859957917	25.375530271783454	24.6213746837932
29	24.32168018964383	25.71828949116388	25.573744587688225	24.386285731504067
30	24.37402240380339	25.467701732182086	25.524587198277594	24.63368866573693
31	24.48276537212446	25.5722349204911	25.325491045713694	24.619508661670743
32	24.430763310635527	25.70438430323611	25.49146373678399	24.373388649344367
33	24.382053640204944	25.528626547693662	25.447876691556708	24.641443120544686
34	24.525066075735776	25.615615447133177	25.2575962930048	24.601722184126253
35	24.47639263546988	25.694196295435034	25.447868087756852	24.38154298133823
36	24.409035138273456	25.534793487574	25.4163518775789	24.639819496573644
37	24.5400778148915	25.632692835653575	25.23774150928288	24.589487840172044
38	24.4859390574618	25.746296273386665	25.443373473470675	24.324391195680864
39	24.431684145079114	25.486310645994358	25.440363412613916	24.641641796312612
40	24.566446401679922	25.56566216520149	25.25877318292472	24.60911825019386
41	24.49029788504153	25.65399650900429	25.45785157101246	24.39785403494172
42	24.473594977862938	25.418368914507944	25.42307702490068	24.68495908272844
43	24.570496064257522	25.499434561198342	25.22267854209219	24.70739083245195
44	24.52181499092805	25.622650941358945	25.438191857986293	24.417342209726712
45	24.48132845427891	25.425965216694152	25.410274360912588	24.68243196811435
46	24.601434140097847	25.47607867390423	25.232365713994415	24.690121472003508
47	24.557275561745612	25.611436514731228	25.38959682548149	24.441691098041666
48	24.539409461372305	25.379612720510664	25.369173900960735	24.711803917156296
49	24.657780077777492	25.468485825474147	25.191725743076532	24.68200835367183
50	24.617482840143442	25.552647097538404	25.391994776875414	24.437875285442733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1169.0
1	791.0
2	413.0
3	381.5
4	350.0
5	376.0
6	402.0
7	482.0
8	562.0
9	860.0
10	1158.0
11	2099.0
12	3040.0
13	4950.0
14	6860.0
15	11169.5
16	15479.0
17	23746.5
18	32014.0
19	45442.0
20	58870.0
21	80523.5
22	102177.0
23	132023.0
24	161869.0
25	203113.0
26	244357.0
27	292357.0
28	340357.0
29	393364.0
30	446371.0
31	516372.0
32	586373.0
33	669869.0
34	753365.0
35	848084.5
36	942804.0
37	1040283.5
38	1137763.0
39	1243913.5
40	1350064.0
41	1445388.0
42	1540712.0
43	1633942.5
44	1727173.0
45	1819450.0
46	1911727.0
47	1991240.5
48	2070754.0
49	2126475.5
50	2182197.0
51	2195828.5
52	2209460.0
53	2168803.5
54	2128147.0
55	2042109.0
56	1956071.0
57	1837770.0
58	1719469.0
59	1573236.5
60	1427004.0
61	1249656.5
62	1072309.0
63	905910.0
64	739511.0
65	608911.0
66	478311.0
67	387054.0
68	295797.0
69	232714.0
70	169631.0
71	131079.5
72	92528.0
73	73156.0
74	53784.0
75	41793.0
76	29802.0
77	23265.0
78	16728.0
79	13284.5
80	9841.0
81	7228.5
82	4616.0
83	3557.5
84	2499.0
85	1833.5
86	1168.0
87	868.0
88	568.0
89	423.5
90	279.0
91	191.5
92	104.0
93	71.5
94	39.0
95	35.5
96	32.0
97	33.5
98	35.0
99	100.5
100	166.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5036523043248089
2	0.0
3	0.0
4	2.854463840883051E-5
5	0.0
6	0.0010989685787399746
7	0.0
8	0.0
9	1.0704239403311443E-5
10	0.0
11	8.456349128616039E-4
12	0.0
13	0.0
14	0.0
15	0.0
16	1.4272319204415255E-5
17	0.0
18	0.0
19	5.352119701655721E-5
20	7.564329178340086E-4
21	2.069486284640212E-4
22	7.600009976351124E-4
23	0.0
24	0.0033682673322420003
25	0.010493722695046318
26	0.020305942148081804
27	0.023709890278334846
28	0.049086073823785166
29	0.05815256459838996
30	0.08078489477679145
31	0.13322139553381313
32	0.15536489877946338
33	0.15986781548845638
34	0.15292790027530948
35	0.1573523192286782
36	0.19212682497023598
37	0.186250197537818
38	0.20729473220472827
39	0.2197651711095861
40	0.2285355112606993
41	0.2530874683720946
42	0.2658683302196485
43	0.28803681002390646
44	0.28631699555977447
45	0.3084212499276126
46	0.3289341407041584
47	0.3303827811034066
48	0.3285701965644458
49	0.32817770778632444
50	0.3284132010531972
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.8026279E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.408958684690724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1220467854896	42.73252304015978
2	12.012178229709999	13.311645744862854
3	4.214348612382514	7.0053800443915915
4	2.0072748499894844	4.448840369275444
5	1.1561215608749538	3.202974590050023
6	0.7192143024337347	2.3910549341393685
7	0.5017779760039952	1.9462096598905174
8	0.36516697095877393	1.618681728549469
9	0.28416218757506795	1.4170617819988451
>10	1.480293195424332	14.706094697665472
>50	0.09836393322176151	3.7031229628216527
>100	0.038810178390174005	3.4145709682521943
>500	2.1526933485145286E-4	0.07677101025277694
>1k	2.5948210675284343E-5	0.02506846768986434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.784039900551907E-5	5.7089276817661026E-5	0.0	3.568079801103814E-6	0.0
2	2.1408478806622886E-5	1.4629127184525637E-4	0.0	7.136159602207628E-6	0.0
3	2.1408478806622886E-5	2.069486284640212E-4	0.0	7.136159602207628E-6	1.0704239403311443E-5
4	2.8544638408830513E-5	2.7474214468499366E-4	0.0	7.136159602207628E-6	2.4976558607726698E-5
5	4.638503741434958E-5	2.8901446388940896E-4	0.0	1.0704239403311443E-5	3.211271820993433E-5
6	6.065735661876484E-5	3.318314215026547E-4	0.0	1.4272319204415257E-5	3.568079801103814E-5
7	6.065735661876484E-5	3.568079801103814E-4	0.0	1.4272319204415257E-5	3.568079801103814E-5
8	6.422543641986866E-5	3.782164589170043E-4	0.0	1.4272319204415257E-5	3.568079801103814E-5
9	8.563391522649154E-5	4.317376559335615E-4	0.0	1.4272319204415257E-5	3.924887781214195E-5
10	3.6394413971258904E-4	4.7098653374570346E-4	0.0	1.784039900551907E-5	3.924887781214195E-5
11	4.2816957613245766E-4	5.209396509611568E-4	0.0	2.4976558607726698E-5	4.638503741434958E-5
12	4.5314613474018437E-4	5.459162095688835E-4	0.0	3.211271820993433E-5	5.352119701655721E-5
13	4.923950125523263E-4	5.815970075799217E-4	0.0	3.211271820993433E-5	7.136159602207628E-5
14	5.38780049966676E-4	6.065735661876484E-4	0.0	3.211271820993433E-5	9.990623443090679E-5
15	7.421605986295933E-4	6.386862843975827E-4	0.0	3.211271820993433E-5	1.1061047383421824E-4
16	8.206583542538773E-4	6.779351622097247E-4	0.0	3.211271820993433E-5	1.1774663343642586E-4
17	9.455411472925107E-4	7.385925188284895E-4	0.0	3.211271820993433E-5	1.3201895264084113E-4
18	0.0011560578555576359	7.742733168395276E-4	0.0	3.211271820993433E-5	1.3915511224304875E-4
19	0.0013487341648172418	8.420668330605001E-4	0.0	3.568079801103814E-5	1.569955112485678E-4
20	0.004927518205324367	9.134284290825764E-4	0.0	3.568079801103814E-5	1.6413167085077543E-4
21	0.005202260350009361	9.59813466496926E-4	0.0	3.568079801103814E-5	1.7126783045298309E-4
22	0.005362823941059033	9.633815462980298E-4	0.0	3.568079801103814E-5	1.7126783045298309E-4
23	0.005537659851313119	9.99062344309068E-4	0.0	3.568079801103814E-5	1.8910822945850215E-4
24	0.005787425437390387	0.001016902743314587	0.0	4.281695761324577E-5	1.9267630925960596E-4
25	0.006022918704263238	0.0010311750625190022	0.0	4.638503741434958E-5	2.033805486629174E-4
26	0.006290524689346024	0.001073992020132248	0.0	4.9953117215453395E-5	2.1408478806622883E-4
27	0.0065474264350254986	0.0011239451373477015	0.0	4.9953117215453395E-5	2.2122094766843648E-4
28	0.009062922694803688	0.0011560578555576359	0.0	5.352119701655721E-5	2.283571072706441E-4
29	0.00940189027590855	0.0011631940151598434	0.0	6.065735661876484E-5	2.3549326687285173E-4
30	0.009876444889455356	0.0011953067333697776	0.0	6.065735661876484E-5	2.4262942647505935E-4
31	0.016356077808259883	0.0012167152121764005	0.0	6.422543641986866E-5	2.4619750627616316E-4
32	0.01680208778339786	0.0012773725687951654	0.0	7.136159602207628E-5	2.6760598508278604E-4
33	0.017426501748591026	0.0013166214466073074	0.0	7.136159602207628E-5	2.9258254369051275E-4
34	0.01784753516512128	0.0013701426436238647	0.0	7.492967582318009E-5	2.961506234916166E-4
35	0.018268568581651528	0.0013951192022315913	0.0	7.492967582318009E-5	3.2826334170155087E-4
36	0.018796644392214894	0.0014629127184525637	0.0	8.563391522649154E-5	3.353995013037585E-4
37	0.01933542444218157	0.0014736169578558752	0.0	8.920199502759535E-5	3.353995013037585E-4
38	0.019941998008369217	0.0015128658356680172	0.0	8.920199502759535E-5	3.353995013037585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	7380	0.0	13.158989	9
CAACGCA	7865	0.0	12.319598	10
ATCAACG	8805	0.0	11.0792465	8
AACGCAG	9015	0.0	10.821161	11
GTATCAA	9360	0.0	10.6572695	6
GGTATCA	9825	0.0	10.242332	5
ACGCAGA	9875	0.0	10.056721	12
AGTGGTA	10150	0.0	9.806106	2
TATCAAC	10820	0.0	9.401786	7
GTGGTAT	10705	0.0	9.400332	3
AGAGTAC	10955	0.0	9.045367	16
CGCAGAG	11250	0.0	8.944747	13
TGGTATC	11975	0.0	8.45843	4
GAGTACA	11570	0.0	7.3491936	17
GTACATG	12405	0.0	7.2452555	19
CAGAGTA	14605	0.0	7.055527	15
AGTACAT	12400	0.0	6.946102	18
CAGTGGT	16345	0.0	6.660606	1
GCAGAGT	17170	0.0	6.3597894	14
TACATGG	14645	0.0	6.1377053	20
>>END_MODULE