FastQCFastQC Report
Fri 27 May 2016
SRR522031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33147811
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT392610.11844221025635751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC191600.019.610512
CAACGCA197200.019.05358113
AAGCAGT227950.017.844871
GGTATCA218450.017.5706758
GAGTACT129650.017.48241420
AACGCAG214750.017.45551514
ATCAACG217350.017.41743511
AGTGGTA224650.017.3207115
GTATCAA221950.017.2737629
ACGCAGA219300.016.9029215
GTGGTAT230700.016.8092086
CGCAGAG231150.015.95085416
TGGTATC243300.015.8935177
AGAGTAC226500.015.79133619
TATCAAC258200.014.91672510
GTACTTT152150.014.89872622
GCAGTGG276300.014.6321533
TATGCCG22350.014.58778543
AGCAGTG289550.014.1068342
AATGCCG60150.013.96587524
Produced by FastQC (version 0.11.5)