FastQCFastQC Report
Fri 27 May 2016
SRR522030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31952187
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT455400.1425254552998203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC195200.020.29329312
CAACGCA199000.019.8174413
GAGTACT136100.019.16944920
AAGCAGT226250.018.7775861
AACGCAG214550.018.43235614
GGTATCA221800.018.371718
ATCAACG222250.017.91034311
GTATCAA226400.017.8819689
AGTGGTA232650.017.7607365
ACGCAGA221100.017.71733915
GTGGTAT236850.017.259986
CGCAGAG233950.016.78185716
TGGTATC245400.016.712367
AGAGTAC228600.016.63315819
TATGCCG23200.016.61576543
AATGCCG61400.016.45028524
GTACTTT157650.016.32907322
GATCGGA66500.015.8143851
GCAGTGG273600.015.3356243
TATCAAC266800.015.25667710
Produced by FastQC (version 0.11.5)