FastQCFastQC Report
Wed 25 May 2016
SRR491090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26962446
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316920.11754126461671911No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT291430.10808737456534917No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286470.10624777885507866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA230450.049.4053461
TCTCGAG285900.039.5999222
CTCGAGG339450.033.1176453
TATCTCG118600.030.8691332
TCGAGGG411950.027.1994424
ATATCTC144700.026.0107121
GATCCGG321750.025.6647661
TATGGAT164550.021.6492582
ATCCGGC393550.020.8982432
CGAGGGC611000.018.4618995
TCCGGCG461550.017.9517843
AGGGCGC629350.017.7891777
GGATCCG226000.017.5927452
GGGCGCG690400.016.2705948
ATGGATC227450.015.7656783
GAGGGCG718400.015.7214936
GCGCCGT523750.015.5697839
TGGATCC262750.015.4539161
CGGCGCG538150.015.3190765
CCGGCGC594000.014.0129764
Produced by FastQC (version 0.11.5)