FastQCFastQC Report
Wed 25 May 2016
SRR491089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31442433
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332200.10565340156723876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA230100.043.8870661
TCTCGAG273150.036.6823732
TATCTCG109450.033.0633162
CTCGAGG342150.029.1203293
TCGAGGG390500.025.4678964
GATCCGG349000.025.3723561
ATCCGGC398150.022.1440752
ATATCTC185250.019.959051
CGAGGGC524450.019.0710625
TCCGGCG475100.018.6962183
TATGGAT221550.017.8612482
AGGGCGC584300.016.9728167
GCGCCGT526650.016.5906989
CGGCGCG538550.016.3113785
GGATCCG244200.015.9159032
GGGCGCG626350.015.8408538
GAGGGCG634850.015.8138476
CCGGCGC591500.014.9462574
ATATGGA274150.014.7581961
CGTCGAC506450.014.12439712-13
Produced by FastQC (version 0.11.5)