FastQCFastQC Report
Wed 25 May 2016
SRR491088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31428586
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270320.4041925398743679No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT372920.1186563086229842No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT354770.11288131130048294No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316900.10083177143254234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG134400.044.8291972
ATCTCGA289600.041.5090521
ATATCTC172500.035.526211
TCTCGAG349550.034.1639062
CTCGAGG421000.028.0532133
GATCCGG421050.025.3393341
TATGGAT195000.024.3903542
TCGAGGG497200.023.7169234
ATCCGGC495850.021.4868812
ATATGGA248750.019.769611
TCCGGCG578300.018.4803183
CGAGGGC662250.017.8486545
GGATCCG299200.016.0217822
GCGCCGT663300.015.8647629
ATGGATC302000.015.842113
AGGGCGC749150.015.6464987
CGGCGCG700000.015.187425
GAGGGCG805050.014.7410356
CCGGCGC730000.014.6727084
TGGATCC346150.014.3292921
Produced by FastQC (version 0.11.5)