##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491088_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31428586 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21620294976045 33.0 31.0 34.0 28.0 34.0 2 31.530534304025004 34.0 31.0 34.0 28.0 34.0 3 31.606414777934965 34.0 31.0 34.0 28.0 34.0 4 35.01635638332568 37.0 35.0 37.0 32.0 37.0 5 34.74891966186452 37.0 35.0 37.0 32.0 37.0 6 34.71433694153469 37.0 35.0 37.0 32.0 37.0 7 34.65768132234775 37.0 35.0 37.0 32.0 37.0 8 34.603284920295174 37.0 35.0 37.0 32.0 37.0 9 36.173443278676295 39.0 37.0 39.0 32.0 39.0 10-11 36.11336906789252 39.0 37.0 39.0 31.5 39.0 12-13 36.06918302019696 39.0 37.0 39.0 31.0 39.0 14-15 37.333888517924414 40.0 38.0 41.0 31.0 41.0 16-17 37.23585211565039 40.0 38.0 41.0 31.0 41.0 18-19 37.20461766876817 40.0 37.5 41.0 31.0 41.0 20-21 37.150376857552544 40.0 37.5 41.0 31.0 41.0 22-23 37.03629924680671 40.0 37.0 41.0 31.0 41.0 24-25 36.95967923914871 40.0 37.0 41.0 30.0 41.0 26-27 36.78279258252344 40.0 37.0 41.0 30.0 41.0 28-29 36.551890339578115 40.0 36.0 41.0 30.0 41.0 30-31 36.369236799262936 40.0 36.0 41.0 30.0 41.0 32-33 36.15121377080089 40.0 35.0 41.0 29.0 41.0 34-35 35.92559331177037 40.0 35.0 41.0 28.0 41.0 36-37 35.75724222846042 39.5 35.0 41.0 27.0 41.0 38-39 35.554047722668784 39.0 35.0 41.0 26.5 41.0 40-41 35.287217710017245 39.0 35.0 41.0 25.5 41.0 42-43 34.90280189506458 39.0 35.0 41.0 24.0 41.0 44-45 34.49430882763863 39.0 34.0 41.0 22.5 41.0 46-47 34.3008875264067 39.0 34.0 41.0 19.5 41.0 48-49 34.05894175449065 39.0 34.0 41.0 16.5 41.0 50-51 33.79475532243163 38.5 33.5 41.0 13.5 41.0 52-53 33.56768155271128 38.0 33.0 40.5 10.5 41.0 54-55 33.29356821843655 38.0 33.0 40.0 8.0 41.0 56-57 32.98659909484951 37.5 33.0 40.0 7.0 41.0 58-59 32.63463475576025 37.0 32.0 40.0 3.5 41.0 60-61 32.32032174466901 36.5 32.0 40.0 2.0 41.0 62-63 32.083047277405356 36.0 32.0 40.0 2.0 41.0 64-65 31.990983526907637 36.0 32.0 39.0 2.0 41.0 66-67 31.722863335308816 35.0 32.0 39.0 2.0 41.0 68-69 31.400480139322845 35.0 31.5 39.0 2.0 41.0 70-71 31.02523285648295 35.0 31.0 37.5 2.0 40.0 72-73 30.64557188478031 35.0 31.0 37.0 2.0 40.0 74-75 30.293043998225055 35.0 31.0 36.5 2.0 39.0 76-77 29.523252382401168 34.0 29.5 36.0 2.0 39.0 78-79 29.645160030425806 35.0 30.0 36.0 2.0 38.5 80-81 29.43100819107802 35.0 30.0 35.0 2.0 37.0 82-83 29.168699858148244 35.0 30.0 35.0 2.0 37.0 84-85 28.89261586569628 35.0 30.0 35.0 2.0 36.5 86-87 28.53056287673903 34.0 29.0 35.0 2.0 36.0 88-89 28.373815322140167 34.0 29.0 35.0 2.0 36.0 90-91 28.11066509641891 34.0 29.0 35.0 2.0 35.5 92-93 27.85265756149513 34.0 29.0 35.0 2.0 35.0 94-95 27.612243834323312 34.0 29.0 35.0 2.0 35.0 96-97 27.313169418439635 34.0 27.0 35.0 2.0 35.0 98-99 26.972209599248277 34.0 27.0 35.0 2.0 35.0 100 26.20640549975745 33.0 24.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5720223550247496 1101 2 0.5743130822904128 1101 3 0.5786792460672423 1101 4 0.5624883788507447 1101 5 0.6797956524409372 1101 6 0.6224633103180963 1101 7 0.6923437398102479 1101 8 0.7056198977560513 1101 9 0.8514516821634217 1101 10-11 0.8423066120403249 1101 12-13 0.7993834407319724 1101 14-15 0.8784706359491565 1101 16-17 0.8870358426220548 1101 18-19 0.9134818230512991 1101 20-21 0.9508202296356814 1101 22-23 0.9094178954212495 1101 24-25 0.9859933038188871 1101 26-27 0.9362286170051988 1101 28-29 0.9428877702006346 1101 30-31 0.9082968047034541 1101 32-33 0.9492404328582325 1101 34-35 0.982085947514598 1101 36-37 1.0333872263817057 1101 38-39 1.0122519745683078 1101 40-41 0.9410675419200913 1101 42-43 1.0436644559122144 1101 44-45 0.979734319081885 1101 46-47 0.9112059942970987 1101 48-49 0.8590794073906594 1101 50-51 0.8527403770399147 1101 52-53 0.829849380539784 1101 54-55 0.8476229845770433 1101 56-57 0.792007883208278 1101 58-59 0.7526358000686173 1101 60-61 0.8577665616053238 1101 62-63 0.8358755780428524 1101 64-65 0.7868084848427799 1101 66-67 0.8468878113657858 1101 68-69 0.8676671087832375 1101 70-71 0.925644031393329 1101 72-73 0.9278682445109041 1101 74-75 0.9198107514411724 1101 76-77 0.9093212592408513 1101 78-79 0.9436750622503389 1101 80-81 0.900502625373754 1101 82-83 0.8996036060610528 1101 84-85 0.8468049421921862 1101 86-87 0.8851073922543904 1101 88-89 0.9158372573953635 1101 90-91 0.9063849469226071 1101 92-93 0.9929973162010448 1101 94-95 0.9164897138835109 1101 96-97 0.9377118932719881 1101 98-99 0.9815861099597534 1101 100 0.9475620152924158 1102 1 0.3444494432660825 1102 2 0.38489181786249915 1102 3 0.38141144681403816 1102 4 0.40050751715926936 1102 5 0.44366910610251153 1102 6 0.4190243765165178 1102 7 0.46941528049421066 1102 8 0.47812863500786307 1102 9 0.5812613358214165 1102 10-11 0.5718440798634461 1102 12-13 0.5493068770783083 1102 14-15 0.5197217070403966 1102 16-17 0.5630344744166607 1102 18-19 0.592978829069331 1102 20-21 0.6194776187593973 1102 22-23 0.6340138731185192 1102 24-25 0.617873317552224 1102 26-27 0.49379135344337044 1102 28-29 0.6026032909711958 1102 30-31 0.6295162497266915 1102 32-33 0.6009076408297531 1102 34-35 0.5787327236023572 1102 36-37 0.65487246362202 1102 38-39 0.6628466940478646 1102 40-41 0.644026493142178 1102 42-43 0.5948644909077032 1102 44-45 0.5847862365530716 1102 46-47 0.6224137349561332 1102 48-49 0.624130125781214 1102 50-51 0.6700679552844804 1102 52-53 0.5682404334480537 1102 54-55 0.5591678475073607 1102 56-57 0.5853123125739046 1102 58-59 0.6729308372127747 1102 60-61 0.6036250847245057 1102 62-63 0.6262339478547432 1102 64-65 0.7123740989870413 1102 66-67 0.6670945338750691 1102 68-69 0.5210843409024193 1102 70-71 0.6294111380973675 1102 72-73 0.5956652339995259 1102 74-75 0.6549138221357396 1102 76-77 0.6235442430073128 1102 78-79 0.5931349889143682 1102 80-81 0.5760312462506754 1102 82-83 0.6620468488574289 1102 84-85 0.6148379525630432 1102 86-87 0.7453668498915036 1102 88-89 0.7120690119298807 1102 90-91 0.6330456309575823 1102 92-93 0.5853441773986887 1102 94-95 0.6951688407670638 1102 96-97 0.5725089590646633 1102 98-99 0.640012075975573 1102 100 0.5964338011110151 1103 1 0.25582851909311444 1103 2 0.25834508727547956 1103 3 0.21786484266910477 1103 4 0.1892493157694588 1103 5 0.3151877289861602 1103 6 0.30070361597672246 1103 7 0.29587327212212244 1103 8 0.3227359712389912 1103 9 0.3629966409857346 1103 10-11 0.4058784133349249 1103 12-13 0.41986425810237904 1103 14-15 0.4568036279850247 1103 16-17 0.4407769223900502 1103 18-19 0.45194896726011535 1103 20-21 0.4323825000712631 1103 22-23 0.4317932571878984 1103 24-25 0.4765652165125758 1103 26-27 0.4579332070249649 1103 28-29 0.41179434301692197 1103 30-31 0.47350824710970585 1103 32-33 0.45207369391052765 1103 34-35 0.4749203471648471 1103 36-37 0.45558823655595404 1103 38-39 0.43950136832535236 1103 40-41 0.39396505711789587 1103 42-43 0.4452612334083881 1103 44-45 0.4983376039248455 1103 46-47 0.43208272994136365 1103 48-49 0.49498017666309835 1103 50-51 0.49956617619953647 1103 52-53 0.4676751920618045 1103 54-55 0.37767268089330486 1103 56-57 0.42897861816743443 1103 58-59 0.46374763029108124 1103 60-61 0.5479348338175072 1103 62-63 0.5897087897306363 1103 64-65 0.5379356935992661 1103 66-67 0.40109700889783184 1103 68-69 0.5034227595779868 1103 70-71 0.5975290587802924 1103 72-73 0.6165436595528213 1103 74-75 0.5829150751747498 1103 76-77 0.5418504756672426 1103 78-79 0.42665995361749864 1103 80-81 0.494438302952215 1103 82-83 0.5677135093478789 1103 84-85 0.634614091775326 1103 86-87 0.6714731540962298 1103 88-89 0.6574263262032751 1103 90-91 0.6746827924250489 1103 92-93 0.6895493309249936 1103 94-95 0.7047993166060564 1103 96-97 0.7249626685503827 1103 98-99 0.7045287428718687 1103 100 0.7240583773979488 1104 1 -0.012617564218782462 1104 2 -0.009957052371323272 1104 3 -0.002020388813381402 1104 4 -0.036568786133962305 1104 5 -0.0037691018836056855 1104 6 0.041526514418322336 1104 7 0.012541975502152525 1104 8 -0.0158753076909548 1104 9 -0.02124655423377675 1104 10-11 0.0074874473494901395 1104 12-13 0.08224900335726204 1104 14-15 0.03796906191153937 1104 16-17 -0.030946520221455387 1104 18-19 -0.011173142751026433 1104 20-21 0.04234142409205077 1104 22-23 -0.012202399441058276 1104 24-25 -0.049762828478854715 1104 26-27 -5.526011018588406E-4 1104 28-29 0.005154197527126314 1104 30-31 -0.018272471532895906 1104 32-33 -0.05111313796662387 1104 34-35 0.03100182343293767 1104 36-37 0.02142327771113628 1104 38-39 -0.028775095105977755 1104 40-41 0.0018458050743674903 1104 42-43 -0.12360582918240937 1104 44-45 -0.09123151962384668 1104 46-47 0.013121278999186359 1104 48-49 -0.020872606108220282 1104 50-51 -0.05580194731914645 1104 52-53 -0.01688160354930801 1104 54-55 -0.07538210490442765 1104 56-57 -0.00906286304117998 1104 58-59 -0.0502521946380341 1104 60-61 -0.16904801474777997 1104 62-63 -0.1601243892186659 1104 64-65 -0.09843103051325386 1104 66-67 0.007582855549149059 1104 68-69 0.003821313418995942 1104 70-71 0.027074898088095267 1104 72-73 0.11104889709751475 1104 74-75 0.07183321300384193 1104 76-77 0.04266565233872299 1104 78-79 0.17627308169640443 1104 80-81 0.09723983368610334 1104 82-83 0.06975740596041291 1104 84-85 0.12984969320004325 1104 86-87 0.20141670344359852 1104 88-89 0.13957477140796115 1104 90-91 0.11944440889735475 1104 92-93 0.26296947970500995 1104 94-95 0.219614774394568 1104 96-97 0.12617209744471936 1104 98-99 0.019353246632913823 1104 100 0.09530953830482503 1105 1 -0.3100045765641646 1105 2 -0.25647692942043676 1105 3 -0.2104037728499364 1105 4 -0.18918680432763324 1105 5 -0.18008579001960356 1105 6 -0.22889011113276325 1105 7 -0.2548247949653941 1105 8 -0.2401390661780809 1105 9 -0.3975657756099409 1105 10-11 -0.3290879054283735 1105 12-13 -0.2862886619456475 1105 14-15 -0.38163987397167887 1105 16-17 -0.29604879830583286 1105 18-19 -0.2599409953087175 1105 20-21 -0.30672648853218476 1105 22-23 -0.2834877429438407 1105 24-25 -0.29614620056803886 1105 26-27 -0.32412272026007827 1105 28-29 -0.387990619112756 1105 30-31 -0.36718244332544003 1105 32-33 -0.32196302194694937 1105 34-35 -0.24701206744820325 1105 36-37 -0.2961791026819398 1105 38-39 -0.45119002700507593 1105 40-41 -0.4156335285069872 1105 42-43 -0.3605197970993501 1105 44-45 -0.26926988403410945 1105 46-47 -0.3000303508669546 1105 48-49 -0.2669665183280756 1105 50-51 -0.2345377980140313 1105 52-53 -0.22852789882561808 1105 54-55 -0.24880967229314166 1105 56-57 -0.2168105339288644 1105 58-59 -0.1999585854105561 1105 60-61 -0.1742170008288184 1105 62-63 -0.10206119979840622 1105 64-65 -0.18232258512928468 1105 66-67 -0.2706397996043144 1105 68-69 -0.2678826319092167 1105 70-71 -0.1849449348523997 1105 72-73 -0.16384785663452917 1105 74-75 -0.19021772772926937 1105 76-77 -0.24017456051393182 1105 78-79 -0.13167751734424726 1105 80-81 -0.1162977311051705 1105 82-83 -0.07316708075295608 1105 84-85 -0.09875037383910623 1105 86-87 -0.060244926363306917 1105 88-89 -0.12052751027055209 1105 90-91 -0.15706442005189913 1105 92-93 -0.16082244049326633 1105 94-95 -0.19797983896519256 1105 96-97 -0.1958253715955287 1105 98-99 -0.13425725514966302 1105 100 -0.15672824643465333 1106 1 -0.5207844805808968 1106 2 -0.5445163340367785 1106 3 -0.44541318689832465 1106 4 -0.3888097710835581 1106 5 -0.4621801139909536 1106 6 -0.5090913842697518 1106 7 -0.5619728513156232 1106 8 -0.4726911355905443 1106 9 -0.7064474563156296 1106 10-11 -0.6308517790211212 1106 12-13 -0.6113898121971957 1106 14-15 -0.7702108298755093 1106 16-17 -0.7272409049625566 1106 18-19 -0.7823168069338493 1106 20-21 -0.7840142677573283 1106 22-23 -0.7531280784733596 1106 24-25 -0.793735158049337 1106 26-27 -0.7566352489379256 1106 28-29 -0.9917525402656722 1106 30-31 -0.9571686290422718 1106 32-33 -0.8836710325316588 1106 34-35 -0.7680985297252505 1106 36-37 -0.8067467455483666 1106 38-39 -0.8630999517281595 1106 40-41 -0.8472691936736254 1106 42-43 -0.8504035158800249 1106 44-45 -0.8533502361123553 1106 46-47 -0.7845521927894978 1106 48-49 -0.9367498951569146 1106 50-51 -0.8304130188467695 1106 52-53 -0.8413617278856975 1106 54-55 -0.8286814156526958 1106 56-57 -0.756694338628563 1106 58-59 -0.6932038235352422 1106 60-61 -0.7358458797797454 1106 62-63 -0.7136739381084851 1106 64-65 -0.766730266244565 1106 66-67 -0.8996826838065992 1106 68-69 -0.7781339081604131 1106 70-71 -0.7088250367175597 1106 72-73 -0.6445725979536512 1106 74-75 -0.7159279022207876 1106 76-77 -0.7458828380478444 1106 78-79 -0.627530719071661 1106 80-81 -0.6059020845727048 1106 82-83 -0.6050028587922718 1106 84-85 -0.5548967658891719 1106 86-87 -0.5316968482782727 1106 88-89 -0.6497988325601689 1106 90-91 -0.6024114078265228 1106 92-93 -0.5796020055881748 1106 94-95 -0.6325224302767971 1106 96-97 -0.6054811187435618 1106 98-99 -0.5611983025911833 1106 100 -0.6039561089208938 1107 1 -0.8210736278146094 1107 2 -0.7769241156652207 1107 3 -0.7726794306217855 1107 4 -0.6743202635102463 1107 5 -0.770205530526276 1107 6 -0.835986198744024 1107 7 -0.9745167988972341 1107 8 -0.9028551612199607 1107 9 -1.068534243772632 1107 10-11 -1.1097020824105854 1107 12-13 -1.1079581300124346 1107 14-15 -1.3252518296356328 1107 16-17 -1.2180888109197454 1107 18-19 -1.2701028706412743 1107 20-21 -1.3678184885716647 1107 22-23 -1.5060098357652407 1107 24-25 -1.4391409597138036 1107 26-27 -1.378136133748228 1107 28-29 -1.4428591382509879 1107 30-31 -1.3652461505594715 1107 32-33 -1.3770719207906765 1107 34-35 -1.405191712970712 1107 36-37 -1.4652280847388326 1107 38-39 -1.5692179109019477 1107 40-41 -1.5269408353808487 1107 42-43 -1.5582982800845073 1107 44-45 -1.4713406725833167 1107 46-47 -1.546812435356209 1107 48-49 -1.383588131382588 1107 50-51 -1.3438111440155183 1107 52-53 -1.3569575957409938 1107 54-55 -1.349798500597018 1107 56-57 -1.4714048179829504 1107 58-59 -1.3559205127767484 1107 60-61 -1.3396499741210306 1107 62-63 -1.318152600278026 1107 64-65 -1.4162969164384975 1107 66-67 -1.4290964770370707 1107 68-69 -1.4449387227010675 1107 70-71 -1.4184094206901143 1107 72-73 -1.4243871308531588 1107 74-75 -1.5252355836191889 1107 76-77 -1.5731566022471029 1107 78-79 -1.5693885500074138 1107 80-81 -1.7199696848923978 1107 82-83 -1.7663975186938679 1107 84-85 -1.8628089749620145 1107 86-87 -1.822447887097713 1107 88-89 -1.7817703875322373 1107 90-91 -1.7747454426636509 1107 92-93 -1.6138051147758468 1107 94-95 -1.6493044288845873 1107 96-97 -1.5742563759496875 1107 98-99 -1.7240592469448224 1107 100 -1.8679241366534143 1108 1 -1.3436263362952054 1108 2 -1.3471464723987552 1108 3 -1.2821630930592036 1108 4 -1.2492959234828618 1108 5 -1.4188856232862577 1108 6 -1.49681691461506 1108 7 -1.4940470276651112 1108 8 -1.539611170789989 1108 9 -1.8195215179359963 1108 10-11 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84-85 0.5295168565431894 2305 86-87 0.5682112093384184 2305 88-89 0.5407570019809178 2305 90-91 0.5566857564691006 2305 92-93 0.5949828913568602 2305 94-95 0.6125554440322425 2305 96-97 0.6211795559226729 2305 98-99 0.6887219520450252 2305 100 0.7463337294114609 2306 1 -0.35977997049626964 2306 2 -0.25288776190171447 2306 3 -0.13432016885097298 2306 4 -0.0926831660979417 2306 5 -0.12725355895498325 2306 6 -0.10484641974820619 2306 7 -0.10488916177890673 2306 8 -0.10701896859207949 2306 9 -0.1053620578130392 2306 10-11 -0.0686378908320151 2306 12-13 -0.07663822314431457 2306 14-15 -0.07903649880461217 2306 16-17 -0.014994472179665763 2306 18-19 -0.07373104997879665 2306 20-21 -0.06283024983267893 2306 22-23 -0.08827266244889387 2306 24-25 -0.10654620768730183 2306 26-27 -0.09119047677599212 2306 28-29 -0.1366980380369469 2306 30-31 -0.10184973973670708 2306 32-33 -0.022622241652058506 2306 34-35 -0.022366828267117 2306 36-37 -0.028437332237757573 2306 38-39 0.004653172651067905 2306 40-41 -0.012806440144053965 2306 42-43 0.039660753256221426 2306 44-45 2.5661070218063742E-5 2306 46-47 -0.013017574479398775 2306 48-49 0.03144678450198768 2306 50-51 0.06125519022307202 2306 52-53 0.0594048422690463 2306 54-55 0.09300836873527629 2306 56-57 0.09548462350997511 2306 58-59 0.10489565084910168 2306 60-61 0.10392458387182302 2306 62-63 0.04878042509761826 2306 64-65 0.11084054267445254 2306 66-67 0.07181222972670653 2306 68-69 0.08180376467620931 2306 70-71 0.129596973769452 2306 72-73 0.23295050428217934 2306 74-75 0.19141017066074184 2306 76-77 0.19052533210819078 2306 78-79 0.12715213545426352 2306 80-81 0.19067175067805664 2306 82-83 0.20339159453385847 2306 84-85 0.2455902087177435 2306 86-87 0.3140331159247438 2306 88-89 0.29221748582714824 2306 90-91 0.29486841241088513 2306 92-93 0.22351565330544787 2306 94-95 0.2506301996723117 2306 96-97 0.32231599066386707 2306 98-99 0.3550024516903214 2306 100 0.34241112181950584 2307 1 -0.4205394192885059 2307 2 -0.392492647918786 2307 3 -0.41699227134870753 2307 4 -0.37385593053603117 2307 5 -0.5909430036270962 2307 6 -0.5380586219118229 2307 7 -0.5502940922056752 2307 8 -0.7207921359656808 2307 9 -0.6466995402900011 2307 10-11 -0.5202475136449394 2307 12-13 -0.5198369220063626 2307 14-15 -0.5817371318445623 2307 16-17 -0.6110181471070106 2307 18-19 -0.6182521583291347 2307 20-21 -0.5923739328337092 2307 22-23 -0.6167104727039501 2307 24-25 -0.6917889354300755 2307 26-27 -0.6361182314872522 2307 28-29 -0.5853222013380659 2307 30-31 -0.6224446386121514 2307 32-33 -0.6065739559572378 2307 34-35 -0.5343012051926195 2307 36-37 -0.5678237072086034 2307 38-39 -0.5174453528476093 2307 40-41 -0.5440793816792464 2307 42-43 -0.5766487380661189 2307 44-45 -0.5245625101800826 2307 46-47 -0.470547393289209 2307 48-49 -0.424039788575449 2307 50-51 -0.45503633769488516 2307 52-53 -0.5203402050709656 2307 54-55 -0.5610516606814286 2307 56-57 -0.4981815080668923 2307 58-59 -0.5816489750746499 2307 60-61 -0.43676851437633957 2307 62-63 -0.5238638803658588 2307 64-65 -0.49757969233326804 2307 66-67 -0.5554803040032077 2307 68-69 -0.49438005409995256 2307 70-71 -0.4972326365671229 2307 72-73 -0.6976093091947426 2307 74-75 -0.7804740934314971 2307 76-77 -0.618368134592405 2307 78-79 -0.6223215239607178 2307 80-81 -0.5881829914621655 2307 82-83 -0.5754142751339621 2307 84-85 -0.7196815532640741 2307 86-87 -0.6996509371305741 2307 88-89 -0.6802565977074018 2307 90-91 -0.5149537731714773 2307 92-93 -0.4757109713085015 2307 94-95 -0.4978149979332791 2307 96-97 -0.8644771128188964 2307 98-99 -0.6781051183572657 2307 100 -0.5645474799989678 2308 1 -1.0702007051611346 2308 2 -1.0716179643771504 2308 3 -1.0142470424235377 2308 4 -1.0115948993368988 2308 5 -1.1655305698207457 2308 6 -1.181959972845597 2308 7 -1.231792838944223 2308 8 -1.3023456670930216 2308 9 -1.3760190501000764 2308 10-11 -1.3734584628306479 2308 12-13 -1.3927226747722798 2308 14-15 -1.53336495480292 2308 16-17 -1.6118203604984558 2308 18-19 -1.729955047876743 2308 20-21 -1.8501401569452867 2308 22-23 -1.8010970876367125 2308 24-25 -1.7585498405885787 2308 26-27 -1.6584066461215912 2308 28-29 -1.6030525011823826 2308 30-31 -1.6734874927777028 2308 32-33 -1.6234996416500493 2308 34-35 -2.1612144498129595 2308 36-37 -2.152012499740387 2308 38-39 -1.7369146439804197 2308 40-41 -1.716028264948818 2308 42-43 -1.6526340684516967 2308 44-45 -1.988030253788736 2308 46-47 -1.8830272208361478 2308 48-49 -2.04538034094022 2308 50-51 -1.6161912376544976 2308 52-53 -1.494766492685585 2308 54-55 -1.5365725987098848 2308 56-57 -1.570692791209865 2308 58-59 -2.635817937734707 2308 60-61 -1.8905081036593359 2308 62-63 -1.8129604078551935 2308 64-65 -1.9836849205755627 2308 66-67 -1.9368606658190224 2308 68-69 -1.9902520145665257 2308 70-71 -1.923946701113291 2308 72-73 -1.8245436242274096 2308 74-75 -1.788491626771691 2308 76-77 -1.5482077669110303 2308 78-79 -1.5125138462824914 2308 80-81 -1.779622492754882 2308 82-83 -1.4904693831544868 2308 84-85 -1.385306973332682 2308 86-87 -1.535116406180066 2308 88-89 -1.560360238923348 2308 90-91 -1.9076751741450195 2308 92-93 -1.816058381814969 2308 94-95 -2.0502454398961767 2308 96-97 -1.8401224143410744 2308 98-99 -1.6474682058413173 2308 100 -1.5189826018496042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 531649.0 3 164122.0 4 94205.0 5 81103.0 6 80595.0 7 88443.0 8 98445.0 9 106268.0 10 115129.0 11 115834.0 12 120631.0 13 147877.0 14 208674.0 15 306766.0 16 390221.0 17 355276.0 18 274202.0 19 195017.0 20 164549.0 21 161741.0 22 173404.0 23 194284.0 24 224193.0 25 265451.0 26 316124.0 27 373994.0 28 444468.0 29 524605.0 30 628635.0 31 760689.0 32 939989.0 33 1196944.0 34 1618000.0 35 2389949.0 36 3817620.0 37 6010098.0 38 6015726.0 39 1726089.0 40 7577.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.038136148192713 27.67240353047356 27.569700804844317 22.719759516489408 2 24.32231599601713 27.07053699456921 26.93018705964054 21.676959949773114 3 24.21118786572199 25.939200700916036 26.290673719778546 23.558937713583425 4 24.85544943184924 25.501810449768808 26.21992890311989 23.42281121526206 5 25.320262985154507 25.568656237242827 25.69079739687588 23.42028338072679 6 25.321661990898264 25.463378040592495 25.22490955650398 23.990050412005257 7 25.193695159823704 25.888220854277012 25.29902062684451 23.619063359054778 8 24.90772750816447 25.493303120696183 25.547352142070327 24.05161722906902 9 24.75537127410458 25.393969377990917 25.600095943565126 24.250563404339378 10-11 24.678666282081764 25.529683687507998 25.584610977938855 24.207039052471384 12-13 24.24810313473988 25.605568168620145 25.82955180045089 24.316776896189086 14-15 23.89113795594838 25.860381906457206 26.38039093822233 23.86808919937208 16-17 23.67133158965517 25.785502384264625 26.679820780253817 23.863345245826387 18-19 23.175359846845986 25.775596984705583 27.53915688513167 23.509886283316757 20-21 22.799138716856245 25.5341993627416 28.475603195465272 23.19105872493688 22-23 22.70608975920836 25.390525101008315 29.000465966433143 22.90291917335019 24-25 22.603786553161846 25.24886148011666 29.521709090043043 22.625642876678455 26-27 22.69403743229963 25.218121795971076 29.683992841840606 22.40384792988869 28-29 22.693122647105845 25.370969892548285 29.593644628520842 22.342262831825025 30-31 22.704907362951985 25.236574299709876 29.60410693092481 22.454411406413328 32-33 22.757044411567104 25.31648983133116 29.44646454670028 22.480001210401454 34-35 22.812693714641334 25.39030740832088 29.420734971784984 22.376263905252802 36-37 22.788688764651415 25.36034915610542 29.374629638780736 22.47633244046243 38-39 22.857188541176196 25.39788059950589 29.222019663157983 22.52291119615993 40-41 22.959122331355665 25.57750554477267 28.950770150846612 22.512601973025053 42-43 23.12915761820806 25.710904219450853 28.43459532904565 22.725342833295432 44-45 23.27009520910085 25.933010942313768 27.927842762817768 22.869051085767612 46-47 23.522646901339204 26.22185134232685 27.319211642217738 22.936290114116208 48-49 23.677575147411975 26.247598022479234 26.849289612861206 23.22553721724759 50-51 23.705163512430826 26.399929245550936 26.55990952238233 23.334997719635908 52-53 23.809505172592722 26.447113890874537 26.410633234666918 23.332747701865824 54-55 23.84280197104868 26.3914770209378 26.313924669680233 23.45179633833329 56-57 23.77814223802811 26.473430259113417 26.29450675604622 23.45392074681225 58-59 23.81305676574742 26.541497379938527 26.232910187905066 23.41253566640899 60-61 23.735357434499534 26.564041667294415 26.15210550145124 23.548495396754817 62-63 23.712516037986912 26.601337549972147 26.18130965682494 23.504836755215997 64-65 23.771261894818764 26.783008312482032 26.0513082866782 23.39442150602101 66-67 23.78491029337918 26.79906586215281 25.926296198036308 23.489727646431703 68-69 23.771951649388782 26.903309388964797 25.83391135214306 23.490827609503363 70-71 23.915973125644683 27.043502094257267 25.69209381371442 23.34843096638364 72-73 23.909262249503087 27.130199784066072 25.579460147360972 23.38107781906987 74-75 23.93488380957781 27.248852616110675 25.555448062351743 23.26081551195977 76-77 23.826165859703046 27.447664573403408 25.430779513969824 23.295390052923725 78-79 23.84475712160997 27.55429231853734 25.34431289104842 23.256637668804274 80-81 23.79994189574518 27.678814571376115 25.27059526949905 23.25064826337965 82-83 23.740500161191644 27.871063102487977 25.227233764284996 23.16120297203538 84-85 23.73540401036836 27.92013697419067 25.09314074099364 23.25131827444733 86-87 23.6579307535127 28.039697705205413 25.046215462813993 23.256156078467896 88-89 23.725968559369313 28.160030811307692 24.903079072057547 23.210921557265447 90-91 23.665151505485298 28.23514520485127 24.80011678836019 23.29958650130325 92-93 23.606577094144697 28.369418004519193 24.7202533141849 23.303751587151204 94-95 23.649100815972577 28.48151563478767 24.600625207909303 23.26875834133045 96-97 23.5919805159632 28.596319818059722 24.431604884042937 23.380094781934137 98-99 23.573404312475446 28.714282993733963 24.315171497297975 23.397141196492612 100 23.643708913214553 28.887840354272758 23.93220335962964 23.53624737288305 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 71061.0 1 39515.0 2 5865.0 3 3338.5 4 2911.5 5 2880.5 6 2919.5 7 3061.0 8 3310.5 9 3683.0 10 4291.5 11 5005.5 12 5945.0 13 8666.0 14 15624.5 15 24295.5 16 41804.5 17 41399.5 18 28626.5 19 33177.5 20 45792.5 21 57892.0 22 69500.0 23 88474.5 24 109053.5 25 132006.0 26 160224.0 27 195231.5 28 236395.5 29 276677.0 30 315224.5 31 358547.5 32 407478.5 33 462397.0 34 519916.5 35 574776.5 36 631172.5 37 696052.5 38 767821.5 39 824618.5 40 863919.5 41 908793.0 42 972163.5 43 1040617.5 44 1098934.0 45 1140708.0 46 1157961.0 47 1162322.5 48 1179930.0 49 1216444.5 50 1202428.0 51 1149080.5 52 1086569.0 53 1001386.5 54 930515.0 55 880023.5 56 830667.0 57 765700.0 58 716187.0 59 676294.5 60 614470.5 61 560028.5 62 507664.0 63 445327.5 64 387722.0 65 329995.0 66 275983.0 67 229729.5 68 187724.0 69 150190.0 70 117372.5 71 92320.0 72 73555.5 73 60786.0 74 50415.5 75 37562.0 76 26525.5 77 20343.0 78 16380.0 79 10899.0 80 5460.5 81 2575.5 82 1344.5 83 793.0 84 504.5 85 318.0 86 227.0 87 170.0 88 130.5 89 96.0 90 62.0 91 45.5 92 35.0 93 23.0 94 18.0 95 15.5 96 11.5 97 6.5 98 2.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22620489512318498 2 0.0 3 0.0 4 0.0015399992860003313 5 6.236360744960018E-4 6 0.0030322713214014784 7 0.003942270899492583 8 0.004963634062315116 9 0.0056572701043565874 10-11 0.007940223591350881 12-13 0.005523633802678873 14-15 0.005498179269025976 16-17 0.006093178993162467 18-19 0.0027856805266390284 20-21 0.0011502267394403298 22-23 0.0011343176559072687 24-25 0.0019424990993867812 26-27 0.0021461353686099654 28-29 0.0029734077123291514 30-31 0.0029686349872692333 32-33 0.0031706803481391112 34-35 0.005991360858550875 36-37 0.004763179609798544 38-39 0.004895225003122954 40-41 0.004656588750127034 42-43 0.002582044257415844 44-45 0.0015845447198929027 46-47 0.001979089991512822 48-49 0.004314543454166217 50-51 0.005049543113393648 52-53 0.003091134930473805 54-55 4.4068161386579723E-4 56-57 2.2590898616947004E-4 58-59 9.38635928450615E-5 60-61 1.6386356039053108E-4 62-63 2.481817031157558E-4 64-65 0.0034665893018540513 66-67 7.89090543239839E-4 68-69 3.515907460806541E-4 70-71 8.27272343719186E-4 72-73 0.0014445447848019635 74-75 7.715905513534716E-4 76-77 0.010089540776667459 78-79 0.003856361848414052 80-81 0.007369087492513981 82-83 5.027270396447362E-4 84-85 0.001512953843994127 86-87 0.0016624992292049027 88-89 0.0037004528297900517 90-91 0.0025343170068166604 92-93 0.001339544833483759 94-95 0.0035652256197590305 96-97 0.007793860022846716 98-99 0.0036193165037714395 100 0.0019027263905541279 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.1428586E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.51687717497338 #Duplication Level Percentage of deduplicated Percentage of total 1 66.620925115089 26.32650915056071 2 17.739249521625435 14.019994890445552 3 7.263576110112709 8.611015349831847 4 3.2207067671424214 5.090890949350907 5 1.481969249928727 2.928139841326047 6 0.8168240650887757 1.9367001752205366 7 0.47484599600526295 1.3135101630817516 8 0.30710263904902624 0.9708589813928444 9 0.22320262452010042 0.793824362846326 >10 1.392946583557529 11.778006541665485 >50 0.2659848880278034 7.312037237570836 >100 0.17955526505235586 13.818101286350368 >500 0.011990371780344592 2.9769843913655296 >1k 9.589668860948296E-4 0.5783257953547617 >5k 8.09180671796419E-6 0.03135275550195658 >10k+ 1.5374432764131947E-4 1.5137481281345972 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 127032 0.4041925398743679 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37292 0.1186563086229842 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 35477 0.11288131130048294 No Hit GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31690 0.10083177143254234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.545450119836763E-6 0.0 0.0 0.0 3.181816706612254E-6 2 9.545450119836763E-6 3.181816706612254E-6 0.0 0.0 6.363633413224508E-6 3 9.545450119836763E-6 3.181816706612254E-6 0.0 3.181816706612254E-6 1.590908353306127E-5 4 1.2727266826449016E-5 3.181816706612254E-6 0.0 3.181816706612254E-6 2.227271694628578E-5 5 2.5454533652898032E-5 3.181816706612254E-6 0.0 3.181816706612254E-6 2.863635035951029E-5 6 3.49999837727348E-5 3.181816706612254E-6 0.0 6.363633413224508E-6 3.181816706612254E-5 7 3.49999837727348E-5 3.181816706612254E-6 0.0 6.363633413224508E-6 3.49999837727348E-5 8 3.818180047934705E-5 3.181816706612254E-6 0.0 9.545450119836763E-6 4.1363617185959305E-5 9 5.0909067305796065E-5 3.181816706612254E-6 0.0 9.545450119836763E-6 5.0909067305796065E-5 10-11 1.0818176802481665E-4 3.181816706612254E-6 0.0 9.545450119836763E-6 5.88636090723267E-5 12-13 4.184088969195114E-4 3.181816706612254E-6 0.0 9.545450119836763E-6 6.681815083885734E-5 14-15 0.0011454540143804115 3.181816706612254E-6 0.0 1.113635847314289E-5 8.27272343719186E-5 16-17 0.00258363516576915 3.181816706612254E-6 3.181816706612254E-6 1.2727266826449016E-5 8.749995943183698E-5 18-19 0.0048061341353378105 4.7727250599183815E-6 3.181816706612254E-6 1.2727266826449016E-5 9.227268449175538E-5 20-21 0.007906814515931452 6.363633413224508E-6 3.181816706612254E-6 1.2727266826449016E-5 1.0818176802481665E-4 22-23 0.012054312593000525 6.363633413224508E-6 3.181816706612254E-6 1.590908353306127E-5 1.2090903485126566E-4 24-25 0.016737946785133764 6.363633413224508E-6 3.181816706612254E-6 1.590908353306127E-5 1.2727266826449016E-4 26-27 0.022223398787333287 6.363633413224508E-6 3.181816706612254E-6 1.590908353306127E-5 1.2727266826449016E-4 28-29 0.02813839604492547 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.352272100310208E-4 30-31 0.03281566660364549 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.3840902673763306E-4 32-33 0.0359608924181317 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.527272019173882E-4 34-35 0.03907430006555179 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.5749992697730656E-4 36-37 0.042337253098182655 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.654544687438372E-4 38-39 0.04523747902625973 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.7977264392359237E-4 40-41 0.04784338690897516 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.8931809404342914E-4 42-43 0.05039361299932488 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.9249991075004136E-4 44-45 0.05323179350162301 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.940908191033475E-4 46-47 0.05611451943781372 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 1.988635441632659E-4 48-49 0.05926769979406646 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 2.1159081098971492E-4 50-51 0.06288542538948459 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 2.2431807781616393E-4 52-53 0.0668595144560433 6.363633413224508E-6 3.181816706612254E-6 1.9090900239673526E-5 2.3227261958269456E-4 54-55 0.07151133048111041 6.363633413224508E-6 3.181816706612254E-6 2.3863625299591906E-5 2.354544362893068E-4 56-57 0.0770636006341488 6.363633413224508E-6 3.181816706612254E-6 2.863635035951029E-5 2.3704534464261294E-4 58-59 0.08405882466363584 6.363633413224508E-6 3.181816706612254E-6 2.863635035951029E-5 2.3863625299591908E-4 60-61 0.09204200278052599 6.363633413224508E-6 3.181816706612254E-6 3.181816706612254E-5 2.4977261146906195E-4 62-63 0.10181495279488552 6.363633413224508E-6 3.181816706612254E-6 3.181816706612254E-5 2.656816950021232E-4 64-65 0.11455017416310107 6.363633413224508E-6 3.181816706612254E-6 3.181816706612254E-5 2.7363623676865384E-4 66-67 0.12970357622834194 6.363633413224508E-6 3.181816706612254E-6 3.181816706612254E-5 2.7522714512196E-4 68-69 0.14822811309423847 6.363633413224508E-6 3.181816706612254E-6 3.181816706612254E-5 2.8159077853518453E-4 70-71 0.17050083004052424 6.363633413224508E-6 3.181816706612254E-6 3.181816706612254E-5 2.8954532030171516E-4 72-73 0.19846422616658604 6.363633413224508E-6 3.181816706612254E-6 3.181816706612254E-5 3.00681678774858E-4 74-75 0.23195761972873996 6.363633413224508E-6 3.181816706612254E-6 3.49999837727348E-5 3.054544038347764E-4 76-77 0.27289010075095327 6.363633413224508E-6 3.181816706612254E-6 3.49999837727348E-5 3.0863622054138864E-4 78-79 0.32314053199848064 6.363633413224508E-6 3.181816706612254E-6 3.49999837727348E-5 3.1340894560130705E-4 80-81 0.38234936818347476 6.363633413224508E-6 3.181816706612254E-6 3.9772708832653175E-5 3.277271207810622E-4 82-83 0.45402456222497567 6.363633413224508E-6 3.181816706612254E-6 4.1363617185959305E-5 3.3409075419428666E-4 84-85 0.5393417953960766 6.363633413224508E-6 3.181816706612254E-6 4.1363617185959305E-5 3.4840892937404184E-4 86-87 0.6393590217517263 6.363633413224508E-6 3.181816706612254E-6 4.1363617185959305E-5 3.643180129071031E-4 88 0.7257501180613088 6.363633413224508E-6 3.181816706612254E-6 4.1363617185959305E-5 3.6590892126040923E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 13440 0.0 44.829197 2 ATCTCGA 28960 0.0 41.509052 1 ATATCTC 17250 0.0 35.52621 1 TCTCGAG 34955 0.0 34.163906 2 CTCGAGG 42100 0.0 28.053213 3 GATCCGG 42105 0.0 25.339334 1 TATGGAT 19500 0.0 24.390354 2 TCGAGGG 49720 0.0 23.716923 4 ATCCGGC 49585 0.0 21.486881 2 ATATGGA 24875 0.0 19.76961 1 TCCGGCG 57830 0.0 18.480318 3 CGAGGGC 66225 0.0 17.848654 5 GGATCCG 29920 0.0 16.021782 2 GCGCCGT 66330 0.0 15.864762 9 ATGGATC 30200 0.0 15.84211 3 AGGGCGC 74915 0.0 15.646498 7 CGGCGCG 70000 0.0 15.18742 5 GAGGGCG 80505 0.0 14.741035 6 CCGGCGC 73000 0.0 14.672708 4 TGGATCC 34615 0.0 14.329292 1 >>END_MODULE