FastQCFastQC Report
Wed 25 May 2016
SRR491087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34622909
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353730.10216645863003597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA259950.042.8030551
TCTCGAG319150.034.9735642
TATCTCG149200.029.4834422
CTCGAGG383900.028.6954573
GATCCGG399050.026.0177021
TCGAGGG448750.024.5603544
ATCCGGC470250.022.026932
ATATCTC214200.021.2223031
TCCGGCG584650.017.8455813
GCGCCGT619900.016.543679
CGGCGCG627850.016.4913925
GGATCCG274900.016.207082
CGAGGGC687250.016.1670745
AGGGCGC681100.016.0715357
TATGGAT268500.015.6657052
GGGCGCG735050.014.9111258
CCGGCGC702000.014.9033634
CGTCGAC595050.014.5290412-13
ATGGATC297800.014.2839291
GAGGGCG791500.014.0911236
Produced by FastQC (version 0.11.5)