FastQCFastQC Report
Wed 25 May 2016
SRR491086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26970469
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA552320.20478694678983891No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT281360.10432150809094198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA225450.041.622041
TATCTCG110750.037.512662
TCTCGAG263450.035.4996642
CTCGAGG327100.028.3621543
ATATCTC158050.027.0927851
GATCCGG317400.024.4439241
TCGAGGG384550.024.0895634
ATCCGGC354950.021.7937532
TATGGAT174000.021.0405712
TCCGGCG416600.018.546213
CGAGGGC508750.018.2089355
ATATGGA213800.017.6044851
GGATCCG217950.016.431132
GCGCCGT467550.016.2146919
AGGGCGC574400.016.0132247
CGGCGCG486700.015.8181215
GAGGGCG612100.015.241956
CCGGCGC528900.014.7246194
GGGCGCG632450.014.5954568
CGTCGAC446400.013.94001812-13
Produced by FastQC (version 0.11.5)