FastQCFastQC Report
Wed 25 May 2016
SRR491085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30718135
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930630.3029578455853521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA200400.040.6703761
TCTCGAG242950.033.2718932
TATCTCG103250.031.0427552
CTCGAGG299300.026.7557323
GATCCGG306700.023.993691
TCGAGGG337550.023.5434074
ATCCGGC340500.021.6004682
ATATCTC166850.019.5675661
TCCGGCG406200.018.19883
CGAGGGC467250.016.8574035
GCGCCGT439600.016.6350569
TATGGAT196950.016.4649012
CGGCGCG460000.015.937965
AGGGCGC510800.015.3282377
GGATCCG212650.014.7630832
CCGGCGC506850.014.5944974
CGTCGAC419200.014.11470612-13
GGGCGCG561500.014.05299958
GAGGGCG569200.013.9288796
TCGACTT447650.013.34887914-15
Produced by FastQC (version 0.11.5)