FastQCFastQC Report
Wed 25 May 2016
SRR491084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29116428
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500110.17176214060323608No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359770.12356254688933684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA212150.041.4146921
ACGTCGA25600.038.8711241
TCTCGAG259750.033.7407952
CGTCGAG30500.031.8935262
TATCTCG123800.029.7216642
CTCGAGG313350.027.8194923
GATCCGG314100.025.2697411
TCGAGGG357250.024.4798744
ATATCTC168150.022.2693861
ATCCGGC367100.021.6210772
TCGTTGC41000.020.9767894
GTCGAGG52850.018.7617133
TCCGGCG442000.018.1062033
CGAGGGC507500.017.4182955
TATGGAT203200.016.188492
GCGCCGT496850.015.8914849
CGGCGCG499750.015.835885
AGGGCGC554650.015.6250057
GGATCCG222300.014.8821342
GGGCGCG596900.014.6372118
Produced by FastQC (version 0.11.5)