FastQCFastQC Report
Wed 25 May 2016
SRR491083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36795118
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1161570.3156859015916188No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416230.11312098523505211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA232650.041.4106331
TCTCGAG285750.033.763752
TATCTCG132450.028.5622122
CTCGAGG341500.027.7837313
GATCCGG358550.025.4902231
TCGAGGG386350.024.474474
ATCCGGC427750.021.3906442
ATATCTC210350.018.6338421
TCCGGCG512700.017.8921073
GCGCCGT540900.016.6390329
CGGCGCG551150.016.431585
CGAGGGC578050.016.3579925
GGATCCG246700.015.887825
AGGGCGC605350.015.5116767
CCGGCGC614100.014.9538034
ATGGATC283950.014.48142053
GGGCGCG649950.014.4761788
CGTCGAC517350.014.40753512-13
TATGGAT295900.014.2619632
GAGGGCG691700.013.7110536
Produced by FastQC (version 0.11.5)