FastQCFastQC Report
Wed 25 May 2016
SRR491082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37418616
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA261350.045.0508351
TCTCGAG323300.036.2819332
TATCTCG136650.029.5760612
CTCGAGG393500.029.438693
TCGAGGG462200.024.9215684
GATCCGG432650.024.371511
ATCCGGC500350.021.0014572
ATATCTC206250.020.1023941
CGAGGGC636350.018.1530485
TCCGGCG584250.018.0738493
TATGGAT262950.018.0509382
AGGGCGC676250.016.9431727
CGGCGCG650850.016.0734085
GCGCCGT659050.015.6884159
GGGCGCG732550.015.6795028
GGATCCG305950.015.3296272
GAGGGCG764050.015.1251766
ATGGATC319700.014.94942953
CCGGCGC724900.014.5675654
ATATGGA355200.013.8479861
Produced by FastQC (version 0.11.5)